Restriction map for
pF1KB9740
(Circular) (Six-base) MAPSORT of: pF1KB9740.seq Check: 8923 from: 1 to: 4664
pF1KB9740 4664 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 540 1536 540
Size: 996 3668
AccI GT'mk_AC
Cuts at: 1322 1702 1322
Size: 380 4284
Acc65I G'GTAC_C
Cuts at: 1680 1680
Size: 4664
AclI AA'CG_TT
Cuts at: 4504 4504
Size: 4664
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 745 2489 2921 3668 3936 745
Size: 1744 432 747 268 1473
Fragments arranged by size:
1744 1473 747 432 268
AflIII A'CryG_T
Cuts at: 2016 3120 4097 2016
Size: 1104 977 2583
Fragments arranged by size:
2583 1104 977
AgeI A'CCGG_T
Cuts at: 3052 3052
Size: 4664
AhdI GACnn_n'nnGTC
Cuts at: 237 237
Size: 4664
AleI CACnn'nnGTG
Cuts at: 259 338 1105 1357 259
Size: 79 767 252 3566
Fragments arranged by size:
3566 767 252 79
AlwNI CAG_nnn'CTG
Cuts at: 243 1158 1623 1969 3536 243
Size: 915 465 346 1567 1371
Fragments arranged by size:
1567 1371 915 465 346
ApaI G_GGCC'C
Cuts at: 442 442
Size: 4664
ApaLI G'TGCA_C
Cuts at: 1628 3434 1628
Size: 1806 2858
ApoI r'AATT_y
Cuts at: 1668 4017 4273 1668
Size: 2349 256 2059
Fragments arranged by size:
2349 2059 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4664
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4664
AvaI C'yCGr_G
Cuts at: 226 419 561 1374 1512 1684 226
Size: 193 142 813 138 172 3206
Fragments arranged by size:
3206 813 193 172 142 138
BamHI G'GATC_C
Cuts at: 1689 1689
Size: 4664
BanI G'GyrC_C
Cuts at: 340 565 1052 1453 1600 1680 2094 2342 2377
Size: 225 487 401 147 80 414 248 35
Cuts at: 2377 340
Size: 2627
Fragments arranged by size:
2627 487 414 401 248 225 147 80
35
BanII G_rGCy'C
Cuts at: 442 915 1317 1678 2708 442
Size: 473 402 361 1030 2398
Fragments arranged by size:
2398 1030 473 402 361
BbeI G_GCGC'C
Cuts at: 1056 1457 2098 2346 1056
Size: 401 641 248 3374
Fragments arranged by size:
3374 641 401 248
BbvCI CC'TCA_GC
Cuts at: 1276 1276
Size: 4664
BciVI GTATCCnnnnn_n'
Cuts at: 2562 3323 4664 2562
Size: 761 1341 2562
Fragments arranged by size:
2562 1341 761
BclI T'GATC_A
Cuts at: 2184 2184
Size: 4664
BglI GCCn_nnn'nGGC
Cuts at: 407 596 407
Size: 189 4475
BglII A'GATC_T
Cuts at: 761 2179 4216 761
Size: 1418 2037 1209
Fragments arranged by size:
2037 1418 1209
BlpI GC'TnA_GC
Cuts at: 1776 1776
Size: 4664
Bme1580I G_kGCm'C
Cuts at: 442 570 580 1603 1632 2289 2382 3438 442
Size: 128 10 1023 29 657 93 1056 1668
Fragments arranged by size:
1668 1056 1023 657 128 93 29 10
BmrI ACTGGGnnnn_n'
Cuts at: 1459 1611 1876 1957 2292 4436 1459
Size: 152 265 81 335 2144 1687
Fragments arranged by size:
2144 1687 335 265 152 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3031 3031
Size: 4664
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1208 1217 1208
Size: 9 4655
Bpu10I CC'TnA_GC
Cuts at: 1276 1276
Size: 4664
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 641 1840 2719 3211 3509 641
Size: 1199 879 492 298 1796
Fragments arranged by size:
1796 1199 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1561 4368 1561
Size: 2807 1857
BsaAI yAC'GTr
Cuts at: 639 2017 639
Size: 1378 3286
BsaBI GATnn'nnATC
Cuts at: 6 2178 4215 6
Size: 2172 2037 455
Fragments arranged by size:
2172 2037 455
BsaHI Gr'CG_yC
Cuts at: 1053 1454 2095 2343 3045 4541 1053
Size: 401 641 248 702 1496 1176
Fragments arranged by size:
1496 1176 702 641 401 248
BsaWI w'CCGG_w
Cuts at: 989 1578 1853 2374 3052 3326 3473 3884 989
Size: 589 275 521 678 274 147 411 1769
Fragments arranged by size:
1769 678 589 521 411 275 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 879 958 961 1024 1429 1599 1602 879
Size: 79 3 63 405 170 3 3941
Fragments arranged by size:
3941 405 170 79 63 3 3
BseYI C'CCAG_C
Cuts at: 349 402 569 629 1139 1151 1282 2089 2847
Size: 53 167 60 510 12 131 807 758
Cuts at: 2847 3424 3920 349
Size: 577 496 1093
Fragments arranged by size:
1093 807 758 577 510 496 167 131
60 53 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 1174 1243 649
Size: 525 69 4070
Fragments arranged by size:
4070 525 69
BsiEI CG_ry'CG
Cuts at: 104 1035 2206 2252 3460 104
Size: 931 1171 46 1208 1308
Fragments arranged by size:
1308 1208 1171 931 46
BsiHKAI G_wGCw'C
Cuts at: 785 1632 1678 2456 2646 3438 785
Size: 847 46 778 190 792 2011
Fragments arranged by size:
2011 847 792 778 190 46
BsmI GAATG_Cn'
Cuts at: 648 648
Size: 4664
Bsp1286I G_dGCh'C
Cuts at: 442 570 580 785 915 1317 1603 1632 1678
Size: 128 10 205 130 402 286 29 46
Cuts at: 1678 2289 2382 2456 2646 2708 3438 442
Size: 611 93 74 190 62 730 1668
Fragments arranged by size:
1668 730 611 402 286 205 190 130
128 93 74 62 46 29 10
BspEI T'CCGG_A
Cuts at: 1578 3884 1578
Size: 2306 2358
BspHI T'CATG_A
Cuts at: 773 3840 773
Size: 3067 1597
BspMI ACCTGCnnnn'nnnn_
Cuts at: 242 371 540 1536 1714 1928 2230 2611 242
Size: 129 169 996 178 214 302 381 2295
Fragments arranged by size:
2295 996 381 302 214 178 169 129
BsrBI CCG'CTC
Cuts at: 50 555 884 1290 2956 3010 4494 4659 50
Size: 505 329 406 1666 54 1484 165 55
Fragments arranged by size:
1666 1484 505 406 329 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2576 2576
Size: 4664
BsrFI r'CCGG_y
Cuts at: 1034 1049 2662 2843 3052 1034
Size: 15 1613 181 209 2646
Fragments arranged by size:
2646 1613 209 181 15
BssSI C'ACGA_G
Cuts at: 2935 3293 2935
Size: 358 4306
BstAPI GCAn_nnn'nTGC
Cuts at: 902 1219 1266 2041 902
Size: 317 47 775 3525
Fragments arranged by size:
3525 775 317 47
BstBI TT'CG_AA
Cuts at: 150 3025 150
Size: 2875 1789
BstXI CCAn_nnnn'nTGG
Cuts at: 1107 1107
Size: 4664
BstYI r'GATC_y
Cuts at: 1 761 1689 2156 2179 2514 2760 3772 3858
Size: 760 928 467 23 335 246 1012 86
Cuts at: 3858 3870 4216 1
Size: 12 346 449
Fragments arranged by size:
1012 928 760 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1007 1494 1007
Size: 487 4177
BtgI C'CryG_G
Cuts at: 396 479 716 1112 1304 396
Size: 83 237 396 192 3756
Fragments arranged by size:
3756 396 237 192 83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 227 2614 2795 2958 227
Size: 2387 181 163 1933
Fragments arranged by size:
2387 1933 181 163
BtsI GCAGTG_nn'
Cuts at: 580 1255 1907 580
Size: 675 652 3337
Fragments arranged by size:
3337 675 652
DraI TTT'AAA
Cuts at: 1663 1663
Size: 4664
DrdI GACnn_nn'nnGTC
Cuts at: 2370 3228 2370
Size: 858 3806
EaeI y'GGCC_r
Cuts at: 294 356 450 998 1109 2249 2423 2814 2841
Size: 62 94 548 111 1140 174 391 27
Cuts at: 2841 4186 294
Size: 1345 772
Fragments arranged by size:
1345 1140 772 548 391 174 111 94
62 27
EagI C'GGCC_G
Cuts at: 2249 2249
Size: 4664
EarI CTCTTCn'nnn_
Cuts at: 958 2687 2897 958
Size: 1729 210 2725
Fragments arranged by size:
2725 1729 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2872 3182 3328 4474 2872
Size: 310 146 1146 3062
Fragments arranged by size:
3062 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1676 1676
Size: 4664
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 745 1208 1217 2489 2921 3668 3936 745
Size: 463 9 1272 432 747 268 1473
Fragments arranged by size:
1473 1272 747 463 432 268 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1411 1411
Size: 4664
EcoO109I rG'GnC_Cy
Cuts at: 231 439 510 972 1080 1490 1803 231
Size: 208 71 462 108 410 313 3092
Fragments arranged by size:
3092 462 410 313 208 108 71
EcoRI G'AATT_C
Cuts at: 1668 1668
Size: 4664
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 801 2683 801
Size: 1882 2782
FspI TGC'GCA
Cuts at: 2445 2445
Size: 4664
HaeII r_GCGC'y
Cuts at: 1056 1457 2098 2346 3368 4347 1056
Size: 401 641 248 1022 979 1373
Fragments arranged by size:
1373 1022 979 641 401 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 112 144 223 255 2506 2536 2538 2568 112
Size: 32 79 32 2251 30 2 30 2208
Fragments arranged by size:
2251 2208 79 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1703 1703
Size: 4664
KasI G'GCGC_C
Cuts at: 1052 1453 2094 2342 1052
Size: 401 641 248 3374
Fragments arranged by size:
3374 641 401 248
KpnI G_GTAC'C
Cuts at: 1684 1684
Size: 4664
MluI A'CGCG_T
Cuts at: 4097 4097
Size: 4664
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 995 1055 3335 3519 995
Size: 60 2280 184 2140
Fragments arranged by size:
2280 2140 184 60
MscI TGG'CCA
Cuts at: 452 1000 2425 452
Size: 548 1425 2691
Fragments arranged by size:
2691 1425 548
MslI CAynn'nnrTG
Cuts at: 259 338 1105 1345 1357 2653 2780 259
Size: 79 767 240 12 1296 127 2143
Fragments arranged by size:
2143 1296 767 240 127 79 12
MspA1I CmG'CkG
Cuts at: 704 1775 2089 2449 3462 3707 704
Size: 1071 314 360 1013 245 1661
Fragments arranged by size:
1661 1071 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1051 2845 1051
Size: 1794 2870
NarI GG'CG_CC
Cuts at: 1053 1454 2095 2343 1053
Size: 401 641 248 3374
Fragments arranged by size:
3374 641 401 248
NcoI C'CATG_G
Cuts at: 396 716 1304 396
Size: 320 588 3756
Fragments arranged by size:
3756 588 320
NgoMIV G'CCGG_C
Cuts at: 1049 2843 1049
Size: 1794 2870
NspI r_CATG'y
Cuts at: 370 429 1717 2217 3124 370
Size: 59 1288 500 907 1910
Fragments arranged by size:
1910 1288 907 500 59
PciI A'CATG_T
Cuts at: 3120 3120
Size: 4664
PflMI CCAn_nnn'nTGG
Cuts at: 1623 1623
Size: 4664
PmeI GTTT'AAAC
Cuts at: 1663 1663
Size: 4664
PmlI CAC'GTG
Cuts at: 639 639
Size: 4664
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3803 3835 3803
Size: 32 4632
PpuMI rG'GwC_Cy
Cuts at: 231 510 972 231
Size: 279 462 3923
Fragments arranged by size:
3923 462 279
PspOMI G'GGCC_C
Cuts at: 438 438
Size: 4664
PstI C_TGCA'G
Cuts at: 239 281 509 1178 1400 1711 2396 239
Size: 42 228 669 222 311 685 2507
Fragments arranged by size:
2507 685 669 311 228 222 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4664
PvuII CAG'CTG
Cuts at: 704 2089 2449 704
Size: 1385 360 2919
Fragments arranged by size:
2919 1385 360
RsrII CG'GwC_CG
Cuts at: 2859 2859
Size: 4664
SacI G_AGCT'C
Cuts at: 1678 1678
Size: 4664
SalI G'TCGA_C
Cuts at: 1701 1701
Size: 4664
SapI GCTCTTCn'nnn_
Cuts at: 2687 2897 2687
Size: 210 4454
SbfI CC_TGCA'GG
Cuts at: 509 1711 509
Size: 1202 3462
ScaI AGT'ACT
Cuts at: 4181 4181
Size: 4664
SexAI A'CCwGG_T
Cuts at: 1196 1196
Size: 4664
SfcI C'TryA_G
Cuts at: 33 235 277 505 765 1174 1319 1396 1646
Size: 202 42 228 260 409 145 77 250
Cuts at: 1646 1707 2392 3385 3576 33
Size: 61 685 993 191 1121
Fragments arranged by size:
1121 993 685 409 260 250 228 202
191 145 77 61 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 596 596
Size: 4664
SfoI GGC'GCC
Cuts at: 1054 1455 2096 2344 1054
Size: 401 641 248 3374
Fragments arranged by size:
3374 641 401 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4664
SmaI CCC'GGG
Cuts at: 228 563 1686 228
Size: 335 1123 3206
Fragments arranged by size:
3206 1123 335
SmlI C'TyrA_G
Cuts at: 656 1819 2734 3226 3488 656
Size: 1163 915 492 262 1832
Fragments arranged by size:
1832 1163 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4664
SphI G_CATG'C
Cuts at: 429 1717 2217 429
Size: 1288 500 2876
Fragments arranged by size:
2876 1288 500
StuI AGG'CCT
Cuts at: 1499 1499
Size: 4664
StyI C'CwwG_G
Cuts at: 344 396 716 1304 1798 2152 344
Size: 52 320 588 494 354 2856
Fragments arranged by size:
2856 588 494 354 320 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 298 350 553 1615 2247 2380 2839 3048 298
Size: 52 203 1062 632 133 459 209 1914
Fragments arranged by size:
1914 1062 632 459 209 203 133 52
TatI w'GTAC_w
Cuts at: 524 4179 524
Size: 3655 1009
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 369 401 4181 4213 369
Size: 32 3780 32 820
Fragments arranged by size:
3780 820 32 32
Tth111I GACn'n_nGTC
Cuts at: 1167 2461 1167
Size: 1294 3370
XbaI T'CTAG_A
Cuts at: 1695 1695
Size: 4664
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 496 1110 2159 496
Size: 614 1049 3001
Fragments arranged by size:
3001 1049 614
XmaI C'CCGG_G
Cuts at: 226 561 1684 226
Size: 335 1123 3206
Fragments arranged by size:
3206 1123 335
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI AscI AvrII BaeI
BbsI BcgI BfrBI BmgBI BmtI BsaXI BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PshAI PsiI PspXI
PsrI SacII SanDI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI