Restriction map for
pF1KB9730
(Circular) (Six-base) MAPSORT of: pF1KB9730.seq Check: 2625 from: 1 to: 4508
pF1KB9730 4508 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 248 248
Size: 4508
AccI GT'mk_AC
Cuts at: 1546 1546
Size: 4508
Acc65I G'GTAC_C
Cuts at: 1524 1524
Size: 4508
AclI AA'CG_TT
Cuts at: 4348 4348
Size: 4508
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2333 2765 3512 3780 2333
Size: 432 747 268 3061
Fragments arranged by size:
3061 747 432 268
AfeI AGC'GCT
Cuts at: 1027 1027
Size: 4508
AflIII A'CryG_T
Cuts at: 1860 2964 3941 1860
Size: 1104 977 2427
Fragments arranged by size:
2427 1104 977
AgeI A'CCGG_T
Cuts at: 2896 2896
Size: 4508
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 256 256
Size: 4508
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1165 1197 1165
Size: 32 4476
AlwNI CAG_nnn'CTG
Cuts at: 441 528 1371 1813 3380 441
Size: 87 843 442 1567 1569
Fragments arranged by size:
1569 1567 843 442 87
ApaI G_GGCC'C
Cuts at: 330 330
Size: 4508
ApaLI G'TGCA_C
Cuts at: 3278 3278
Size: 4508
ApoI r'AATT_y
Cuts at: 1512 3861 4117 1512
Size: 2349 256 1903
Fragments arranged by size:
2349 1903 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4508
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4508
AvaI C'yCGr_G
Cuts at: 714 911 1528 714
Size: 197 617 3694
Fragments arranged by size:
3694 617 197
BamHI G'GATC_C
Cuts at: 1533 1533
Size: 4508
BanI G'GyrC_C
Cuts at: 168 831 1524 1938 2186 2221 168
Size: 663 693 414 248 35 2455
Fragments arranged by size:
2455 693 663 414 248 35
BanII G_rGCy'C
Cuts at: 330 698 920 1022 1522 2552 330
Size: 368 222 102 500 1030 2286
Fragments arranged by size:
2286 1030 500 368 222 102
BbeI G_GCGC'C
Cuts at: 1942 2190 1942
Size: 248 4260
BbsI GAAGACnn'nnnn_
Cuts at: 1272 1272
Size: 4508
BciVI GTATCCnnnnn_n'
Cuts at: 2406 3167 4508 2406
Size: 761 1341 2406
Fragments arranged by size:
2406 1341 761
BclI T'GATC_A
Cuts at: 2028 2028
Size: 4508
BglI GCCn_nnn'nGGC
Cuts at: 347 533 347
Size: 186 4322
BglII A'GATC_T
Cuts at: 2023 4060 2023
Size: 2037 2471
BlpI GC'TnA_GC
Cuts at: 1206 1384 1620 1206
Size: 178 236 4094
Fragments arranged by size:
4094 236 178
Bme1580I G_kGCm'C
Cuts at: 173 330 836 907 2133 2226 3282 173
Size: 157 506 71 1226 93 1056 1399
Fragments arranged by size:
1399 1226 1056 506 157 93 71
BmgBI CAC'GTC
Cuts at: 621 621
Size: 4508
BmrI ACTGGGnnnn_n'
Cuts at: 1720 1801 2136 4280 1720
Size: 81 335 2144 1948
Fragments arranged by size:
2144 1948 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1470 2875 1470
Size: 1405 3103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 639 1137 639
Size: 498 4010
Bpu10I CC'TnA_GC
Cuts at: 332 332
Size: 4508
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1006 1684 2563 3055 3353 1006
Size: 678 879 492 298 2161
Fragments arranged by size:
2161 879 678 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1135 4212 1135
Size: 3077 1431
BsaAI yAC'GTr
Cuts at: 1861 1861
Size: 4508
BsaBI GATnn'nnATC
Cuts at: 6 2022 4059 6
Size: 2016 2037 455
Fragments arranged by size:
2037 2016 455
BsaHI Gr'CG_yC
Cuts at: 274 1939 2187 2889 4385 274
Size: 1665 248 702 1496 397
Fragments arranged by size:
1665 1496 702 397 248
BsaWI w'CCGG_w
Cuts at: 661 1148 1697 2218 2896 3170 3317 3728 661
Size: 487 549 521 678 274 147 411 1441
Fragments arranged by size:
1441 678 549 521 487 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 244 274 1165 1195 244
Size: 30 891 30 3557
Fragments arranged by size:
3557 891 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1437 1437
Size: 4508
BseYI C'CCAG_C
Cuts at: 189 686 1364 1371 1933 2691 3268 3764 189
Size: 497 678 7 562 758 577 496 933
Fragments arranged by size:
933 758 678 577 562 497 496 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 130
Size: 4508
BsiEI CG_ry'CG
Cuts at: 104 137 789 824 1137 2050 2096 3304 104
Size: 33 652 35 313 913 46 1208 1308
Fragments arranged by size:
1308 1208 913 652 313 46 35 33
BsiHKAI G_wGCw'C
Cuts at: 338 1022 1067 1390 1522 2300 2490 3282 338
Size: 684 45 323 132 778 190 792 1564
Fragments arranged by size:
1564 792 778 684 323 190 132 45
Bsp1286I G_dGCh'C
Cuts at: 173 330 338 698 836 907 920 1022 1067
Size: 157 8 360 138 71 13 102 45
Cuts at: 1067 1390 1522 2133 2226 2300 2490 2552 3282
Size: 323 132 611 93 74 190 62 730
Cuts at: 3282 173
Size: 1399
Fragments arranged by size:
1399 730 611 360 323 190 157 138
132 102 93 74 71 62 45 13
8
BspEI T'CCGG_A
Cuts at: 1148 3728 1148
Size: 2580 1928
BspHI T'CATG_A
Cuts at: 1406 3684 1406
Size: 2278 2230
BspMI ACCTGCnnnn'nnnn_
Cuts at: 194 248 933 1103 1558 1772 2074 2455 194
Size: 54 685 170 455 214 302 381 2247
Fragments arranged by size:
2247 685 455 381 302 214 170 54
BsrBI CCG'CTC
Cuts at: 50 545 1073 2800 2854 4338 4503 50
Size: 495 528 1727 54 1484 165 55
Fragments arranged by size:
1727 1484 528 495 165 55 54
BsrDI GCAATG_nn'
Cuts at: 957 2420 957
Size: 1463 3045
BsrFI r'CCGG_y
Cuts at: 165 346 625 823 934 2506 2687 2896 165
Size: 181 279 198 111 1572 181 209 1777
Fragments arranged by size:
1777 1572 279 209 198 181 181 111
BssHII G'CGCG_C
Cuts at: 208 938 208
Size: 730 3778
BssSI C'ACGA_G
Cuts at: 2779 3137 2779
Size: 358 4150
BstAPI GCAn_nnn'nTGC
Cuts at: 441 1062 1885 441
Size: 621 823 3064
Fragments arranged by size:
3064 823 621
BstBI TT'CG_AA
Cuts at: 761 2869 761
Size: 2108 2400
BstEII G'GTnAC_C
Cuts at: 930 930
Size: 4508
BstXI CCAn_nnnn'nTGG
Cuts at: 270 372 996 270
Size: 102 624 3782
Fragments arranged by size:
3782 624 102
BstYI r'GATC_y
Cuts at: 1 1533 2000 2023 2358 2604 3616 3702 3714
Size: 1532 467 23 335 246 1012 86 12
Cuts at: 3714 4060 1
Size: 346 449
Fragments arranged by size:
1532 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 125 218 228 263 371 792 888 125
Size: 93 10 35 108 421 96 3745
Fragments arranged by size:
3745 421 108 96 93 35 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2458 2639 2802 2458
Size: 181 163 4164
Fragments arranged by size:
4164 181 163
BtsI GCAGTG_nn'
Cuts at: 1751 1751
Size: 4508
DraI TTT'AAA
Cuts at: 1507 1507
Size: 4508
DraIII CAC_nnn'GTG
Cuts at: 153 153
Size: 4508
DrdI GACnn_nn'nnGTC
Cuts at: 2214 3072 2214
Size: 858 3650
EaeI y'GGCC_r
Cuts at: 344 623 776 786 821 1134 2093 2267 2658
Size: 279 153 10 35 313 959 174 391
Cuts at: 2658 2685 4030 344
Size: 27 1345 822
Fragments arranged by size:
1345 959 822 391 313 279 174 153
35 27 10
EagI C'GGCC_G
Cuts at: 786 821 1134 2093 786
Size: 35 313 959 3201
Fragments arranged by size:
3201 959 313 35
EarI CTCTTCn'nnn_
Cuts at: 971 2531 2741 971
Size: 1560 210 2738
Fragments arranged by size:
2738 1560 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 317 377 2716 3026 3172 4318 317
Size: 60 2339 310 146 1146 507
Fragments arranged by size:
2339 1146 507 310 146 60
EcoICRI GAG'CTC
Cuts at: 1020 1520 1020
Size: 500 4008
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 639 1137 2333 2765 3512 3780 639
Size: 498 1196 432 747 268 1367
Fragments arranged by size:
1367 1196 747 498 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1149 1149
Size: 4508
EcoO109I rG'GnC_Cy
Cuts at: 327 1082 1647 327
Size: 755 565 3188
Fragments arranged by size:
3188 755 565
EcoRI G'AATT_C
Cuts at: 1512 1512
Size: 4508
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2527 2527
Size: 4508
FseI GG_CCGG'CC
Cuts at: 350 350
Size: 4508
FspI TGC'GCA
Cuts at: 1102 2289 1102
Size: 1187 3321
HaeII r_GCGC'y
Cuts at: 528 1029 1942 2190 3212 4191 528
Size: 501 913 248 1022 979 845
Fragments arranged by size:
1022 979 913 845 501 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2350 2380 2382 2412 2350
Size: 30 2 30 4446
Fragments arranged by size:
4446 30 30 2
HincII GTy'rAC
Cuts at: 1547 1547
Size: 4508
KasI G'GCGC_C
Cuts at: 1938 2186 1938
Size: 248 4260
KpnI G_GTAC'C
Cuts at: 1528 1528
Size: 4508
MluI A'CGCG_T
Cuts at: 3941 3941
Size: 4508
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1358 3179 3363 1358
Size: 1821 184 2503
Fragments arranged by size:
2503 1821 184
MscI TGG'CCA
Cuts at: 2269 2269
Size: 4508
MslI CAynn'nnrTG
Cuts at: 268 370 559 2497 2624 268
Size: 102 189 1938 127 2152
Fragments arranged by size:
2152 1938 189 127 102
MspA1I CmG'CkG
Cuts at: 120 230 296 441 1371 1619 1933 2293 3306
Size: 110 66 145 930 248 314 360 1013
Cuts at: 3306 3551 120
Size: 245 1077
Fragments arranged by size:
1077 1013 930 360 314 248 245 145
110 66
NaeI GCC'GGC
Cuts at: 348 627 825 2689 348
Size: 279 198 1864 2167
Fragments arranged by size:
2167 1864 279 198
NarI GG'CG_CC
Cuts at: 1939 2187 1939
Size: 248 4260
NcoI C'CATG_G
Cuts at: 125 263 371 125
Size: 138 108 4262
Fragments arranged by size:
4262 138 108
NgoMIV G'CCGG_C
Cuts at: 346 625 823 2687 346
Size: 279 198 1864 2167
Fragments arranged by size:
2167 1864 279 198
NspI r_CATG'y
Cuts at: 405 1194 1217 1312 1561 2061 2968 405
Size: 789 23 95 249 500 907 1945
Fragments arranged by size:
1945 907 789 500 249 95 23
PasI CC'CwG_GG
Cuts at: 882 1077 882
Size: 195 4313
PciI A'CATG_T
Cuts at: 2964 2964
Size: 4508
PflMI CCAn_nnn'nTGG
Cuts at: 528 1115 1371 528
Size: 587 256 3665
Fragments arranged by size:
3665 587 256
PmeI GTTT'AAAC
Cuts at: 1507 1507
Size: 4508
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1165 1197 3647 3679 1165
Size: 32 2450 32 1994
Fragments arranged by size:
2450 1994 32 32
PpuMI rG'GwC_Cy
Cuts at: 1082 1082
Size: 4508
PspOMI G'GGCC_C
Cuts at: 326 326
Size: 4508
PstI C_TGCA'G
Cuts at: 437 1172 1555 2240 437
Size: 735 383 685 2705
Fragments arranged by size:
2705 735 685 383
PvuI CG_AT'CG
Cuts at: 104 137 104
Size: 33 4475
PvuII CAG'CTG
Cuts at: 120 1371 1933 2293 120
Size: 1251 562 360 2335
Fragments arranged by size:
2335 1251 562 360
RsrII CG'GwC_CG
Cuts at: 2703 2703
Size: 4508
SacI G_AGCT'C
Cuts at: 1022 1522 1022
Size: 500 4008
SacII CC_GC'GG
Cuts at: 231 231
Size: 4508
SalI G'TCGA_C
Cuts at: 1545 1545
Size: 4508
SanDI GG'GwC_CC
Cuts at: 1082 1082
Size: 4508
SapI GCTCTTCn'nnn_
Cuts at: 2531 2741 2531
Size: 210 4298
SbfI CC_TGCA'GG
Cuts at: 1555 1555
Size: 4508
ScaI AGT'ACT
Cuts at: 4025 4025
Size: 4508
SfcI C'TryA_G
Cuts at: 33 433 1029 1168 1551 2236 3229 3420 33
Size: 400 596 139 383 685 993 191 1121
Fragments arranged by size:
1121 993 685 596 400 383 191 139
SfoI GGC'GCC
Cuts at: 1940 2188 1940
Size: 248 4260
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4508
SmaI CCC'GGG
Cuts at: 716 913 1530 716
Size: 197 617 3694
Fragments arranged by size:
3694 617 197
SmlI C'TyrA_G
Cuts at: 1021 1663 2578 3070 3332 1021
Size: 642 915 492 262 2197
Fragments arranged by size:
2197 915 642 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4508
SphI G_CATG'C
Cuts at: 405 1217 1561 2061 405
Size: 812 344 500 2852
Fragments arranged by size:
2852 812 500 344
SspI AAT'ATT
Cuts at: 1316 1316
Size: 4508
StyI C'CwwG_G
Cuts at: 125 263 371 1642 1996 125
Size: 138 108 1271 354 2637
Fragments arranged by size:
2637 1271 354 138 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1275 2091 2224 2683 2892 1275
Size: 816 133 459 209 2891
Fragments arranged by size:
2891 816 459 209 133
TatI w'GTAC_w
Cuts at: 4023 4023
Size: 4508
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 244 276 4025 4057 244
Size: 32 3749 32 695
Fragments arranged by size:
3749 695 32 32
Tth111I GACn'n_nGTC
Cuts at: 2305 2305
Size: 4508
XbaI T'CTAG_A
Cuts at: 1539 1539
Size: 4508
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 369 663 2003 369
Size: 294 1340 2874
Fragments arranged by size:
2874 1340 294
XmaI C'CCGG_G
Cuts at: 714 911 1528 714
Size: 197 617 3694
Fragments arranged by size:
3694 617 197
XmnI GAAnn'nnTTC
Cuts at: 960 960
Size: 4508
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AscI AvrII BaeI BbvCI
BcgI BfrBI BmtI BsiWI BsmI BsmBI BsrGI BstZ17I
Bsu36I ClaI CspCI EcoRV FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PfoI PmlI
PshAI PsiI PspXI PsrI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI