Restriction map for
pF1KB9729
(Circular) (Six-base) MAPSORT of: pF1KB9729.seq Check: 2423 from: 1 to: 4451
pF1KB9729 4451 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1432 1432
Size: 4451
AccI GT'mk_AC
Cuts at: 270 1489 270
Size: 1219 3232
Acc65I G'GTAC_C
Cuts at: 1467 1467
Size: 4451
AclI AA'CG_TT
Cuts at: 4291 4291
Size: 4451
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 935 2276 2708 3455 3723 935
Size: 1341 432 747 268 1663
Fragments arranged by size:
1663 1341 747 432 268
AfeI AGC'GCT
Cuts at: 1191 1191
Size: 4451
AflIII A'CryG_T
Cuts at: 1803 2907 3884 1803
Size: 1104 977 2370
Fragments arranged by size:
2370 1104 977
AgeI A'CCGG_T
Cuts at: 2839 2839
Size: 4451
AhdI GACnn_n'nnGTC
Cuts at: 1285 1285
Size: 4451
AleI CACnn'nnGTG
Cuts at: 682 682
Size: 4451
AlwNI CAG_nnn'CTG
Cuts at: 156 981 1756 3323 156
Size: 825 775 1567 1284
Fragments arranged by size:
1567 1284 825 775
ApaLI G'TGCA_C
Cuts at: 1202 3221 1202
Size: 2019 2432
ApoI r'AATT_y
Cuts at: 424 1455 3804 4060 424
Size: 1031 2349 256 815
Fragments arranged by size:
2349 1031 815 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4451
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4451
AvaI C'yCGr_G
Cuts at: 569 851 1004 1471 569
Size: 282 153 467 3549
Fragments arranged by size:
3549 467 282 153
AvrII C'CTAG_G
Cuts at: 1117 1219 1117
Size: 102 4349
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 118 151 118
Size: 33 4418
BamHI G'GATC_C
Cuts at: 1476 1476
Size: 4451
BanI G'GyrC_C
Cuts at: 301 544 713 744 1467 1881 2129 2164 301
Size: 243 169 31 723 414 248 35 2588
Fragments arranged by size:
2588 723 414 248 243 169 35 31
BanII G_rGCy'C
Cuts at: 389 860 1465 2495 389
Size: 471 605 1030 2345
Fragments arranged by size:
2345 1030 605 471
BbeI G_GCGC'C
Cuts at: 305 548 717 748 1885 2133 305
Size: 243 169 31 1137 248 2623
Fragments arranged by size:
2623 1137 248 243 169 31
BbsI GAAGACnn'nnnn_
Cuts at: 884 923 1307 884
Size: 39 384 4028
Fragments arranged by size:
4028 384 39
BciVI GTATCCnnnnn_n'
Cuts at: 2349 3110 4451 2349
Size: 761 1341 2349
Fragments arranged by size:
2349 1341 761
BclI T'GATC_A
Cuts at: 1971 1971
Size: 4451
BglII A'GATC_T
Cuts at: 1966 4003 1966
Size: 2037 2414
BlpI GC'TnA_GC
Cuts at: 1563 1563
Size: 4451
Bme1580I G_kGCm'C
Cuts at: 408 1206 1390 2076 2169 3225 408
Size: 798 184 686 93 1056 1634
Fragments arranged by size:
1634 1056 798 686 184 93
BmrI ACTGGGnnnn_n'
Cuts at: 144 1663 1744 2079 4223 144
Size: 1519 81 335 2144 372
Fragments arranged by size:
2144 1519 372 335 81
BmtI G_CTAG'C
Cuts at: 842 842
Size: 4451
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 408 813 2818 408
Size: 405 2005 2041
Fragments arranged by size:
2041 2005 405
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 904 1451 904
Size: 547 3904
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 703 1627 2506 2998 3296 574
Size: 129 924 879 492 298 1729
Fragments arranged by size:
1729 924 879 492 298 129
BsaI GGTCTCn'nnnn_
Cuts at: 568 1169 4155 568
Size: 601 2986 864
Fragments arranged by size:
2986 864 601
BsaAI yAC'GTr
Cuts at: 1804 1804
Size: 4451
BsaBI GATnn'nnATC
Cuts at: 6 1965 4002 6
Size: 1959 2037 455
Fragments arranged by size:
2037 1959 455
BsaHI Gr'CG_yC
Cuts at: 160 302 545 714 745 1882 2130 2832 4328
Size: 142 243 169 31 1137 248 702 1496
Cuts at: 4328 160
Size: 283
Fragments arranged by size:
1496 1137 702 283 248 243 169 142
31
BsaWI w'CCGG_w
Cuts at: 291 1072 1640 2161 2839 3113 3260 3671 291
Size: 781 568 521 678 274 147 411 1071
Fragments arranged by size:
1071 781 678 568 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 781 800 805 907 910 913 916 919 937
Size: 19 5 102 3 3 3 3 18
Cuts at: 937 1410 781
Size: 473 3822
Fragments arranged by size:
3822 473 102 19 18 5 3 3
3 3
BseYI C'CCAG_C
Cuts at: 234 276 297 940 1209 1876 2634 3211 3707
Size: 42 21 643 269 667 758 577 496
Cuts at: 3707 234
Size: 978
Fragments arranged by size:
978 758 667 643 577 496 269 42
21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1432 1432
Size: 4451
BsiEI CG_ry'CG
Cuts at: 104 292 338 552 1993 2039 3247 104
Size: 188 46 214 1441 46 1208 1308
Fragments arranged by size:
1441 1308 1208 214 188 46 46
BsiHKAI G_wGCw'C
Cuts at: 327 611 1206 1465 2243 2433 3225 327
Size: 284 595 259 778 190 792 1553
Fragments arranged by size:
1553 792 778 595 284 259 190
Bsp1286I G_dGCh'C
Cuts at: 327 389 408 611 860 1206 1390 1465 2076
Size: 62 19 203 249 346 184 75 611
Cuts at: 2076 2169 2243 2433 2495 3225 327
Size: 93 74 190 62 730 1553
Fragments arranged by size:
1553 730 611 346 249 203 190 184
93 75 74 62 62 19
BspEI T'CCGG_A
Cuts at: 1072 3671 1072
Size: 2599 1852
BspHI T'CATG_A
Cuts at: 3627 3627
Size: 4451
BspMI ACCTGCnnnn'nnnn_
Cuts at: 377 1432 1501 1715 2017 2398 377
Size: 1055 69 214 302 381 2430
Fragments arranged by size:
2430 1055 381 302 214 69
BsrBI CCG'CTC
Cuts at: 50 244 492 2743 2797 4281 4446 50
Size: 194 248 2251 54 1484 165 55
Fragments arranged by size:
2251 1484 248 194 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2363 2363
Size: 4451
BsrFI r'CCGG_y
Cuts at: 547 710 2449 2630 2839 547
Size: 163 1739 181 209 2159
Fragments arranged by size:
2159 1739 209 181 163
BssHII G'CGCG_C
Cuts at: 971 971
Size: 4451
BssSI C'ACGA_G
Cuts at: 2722 3080 2722
Size: 358 4093
BstAPI GCAn_nnn'nTGC
Cuts at: 182 331 981 1031 1828 182
Size: 149 650 50 797 2805
Fragments arranged by size:
2805 797 650 149 50
BstBI TT'CG_AA
Cuts at: 2812 2812
Size: 4451
BstYI r'GATC_y
Cuts at: 1 1476 1943 1966 2301 2547 3559 3645 3657
Size: 1475 467 23 335 246 1012 86 12
Cuts at: 3657 4003 1
Size: 346 449
Fragments arranged by size:
1475 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 138 1390 138
Size: 1252 3199
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2401 2582 2745 2401
Size: 181 163 4107
Fragments arranged by size:
4107 181 163
BtsI GCAGTG_nn'
Cuts at: 288 536 979 1420 1694 288
Size: 248 443 441 274 3045
Fragments arranged by size:
3045 443 441 274 248
DraI TTT'AAA
Cuts at: 1450 1450
Size: 4451
DrdI GACnn_nn'nnGTC
Cuts at: 2157 3015 2157
Size: 858 3593
EaeI y'GGCC_r
Cuts at: 335 653 932 2036 2210 2601 2628 3973 335
Size: 318 279 1104 174 391 27 1345 813
Fragments arranged by size:
1345 1104 813 391 318 279 174 27
EagI C'GGCC_G
Cuts at: 335 2036 335
Size: 1701 2750
EarI CTCTTCn'nnn_
Cuts at: 884 2474 2684 884
Size: 1590 210 2651
Fragments arranged by size:
2651 1590 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1117 2659 2969 3115 4261 1117
Size: 1542 310 146 1146 1307
Fragments arranged by size:
1542 1307 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1463 1463
Size: 4451
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 904 935 1451 2276 2708 3455 3723 904
Size: 31 516 825 432 747 268 1632
Fragments arranged by size:
1632 825 747 516 432 268 31
EcoNI CCTnn'n_nnAGG
Cuts at: 679 679
Size: 4451
EcoO109I rG'GnC_Cy
Cuts at: 1590 1590
Size: 4451
EcoRI G'AATT_C
Cuts at: 1455 1455
Size: 4451
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2470 2470
Size: 4451
FspI TGC'GCA
Cuts at: 255 2232 255
Size: 1977 2474
HaeII r_GCGC'y
Cuts at: 146 241 305 548 717 748 1193 1885 2133
Size: 95 64 243 169 31 445 692 248
Cuts at: 2133 3155 4134 146
Size: 1022 979 463
Fragments arranged by size:
1022 979 692 463 445 248 243 169
95 64 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 205 237 1081 1113 1271 1303 2293 2323 2325
Size: 32 844 32 158 32 990 30 2
Cuts at: 2325 2355 205
Size: 30 2301
Fragments arranged by size:
2301 990 844 158 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 271 1124 1490 271
Size: 853 366 3232
Fragments arranged by size:
3232 853 366
KasI G'GCGC_C
Cuts at: 301 544 713 744 1881 2129 301
Size: 243 169 31 1137 248 2623
Fragments arranged by size:
2623 1137 248 243 169 31
KpnI G_GTAC'C
Cuts at: 1471 1471
Size: 4451
MluI A'CGCG_T
Cuts at: 3884 3884
Size: 4451
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3122 3306 3122
Size: 184 4267
MscI TGG'CCA
Cuts at: 655 934 2212 655
Size: 279 1278 2894
Fragments arranged by size:
2894 1278 279
MslI CAynn'nnrTG
Cuts at: 667 682 2440 2567 667
Size: 15 1758 127 2551
Fragments arranged by size:
2551 1758 127 15
MspA1I CmG'CkG
Cuts at: 140 340 346 940 1371 1392 1562 1876 2236
Size: 200 6 594 431 21 170 314 360
Cuts at: 2236 3249 3494 140
Size: 1013 245 1097
Fragments arranged by size:
1097 1013 594 431 360 314 245 200
170 21 6
NaeI GCC'GGC
Cuts at: 712 2632 712
Size: 1920 2531
NarI GG'CG_CC
Cuts at: 302 545 714 745 1882 2130 302
Size: 243 169 31 1137 248 2623
Fragments arranged by size:
2623 1137 248 243 169 31
NgoMIV G'CCGG_C
Cuts at: 710 2630 710
Size: 1920 2531
NheI G'CTAG_C
Cuts at: 838 838
Size: 4451
NotI GC'GGCC_GC
Cuts at: 335 335
Size: 4451
NspI r_CATG'y
Cuts at: 185 1504 2004 2911 185
Size: 1319 500 907 1725
Fragments arranged by size:
1725 1319 907 500
PciI A'CATG_T
Cuts at: 2907 2907
Size: 4451
PflMI CCAn_nnn'nTGG
Cuts at: 124 156 124
Size: 32 4419
PfoI T'CCnGG_A
Cuts at: 1251 1251
Size: 4451
PmeI GTTT'AAAC
Cuts at: 1450 1450
Size: 4451
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3590 3622 3590
Size: 32 4419
PstI C_TGCA'G
Cuts at: 345 441 1002 1361 1498 2183 345
Size: 96 561 359 137 685 2613
Fragments arranged by size:
2613 685 561 359 137 96
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4451
PvuII CAG'CTG
Cuts at: 346 940 1876 2236 346
Size: 594 936 360 2561
Fragments arranged by size:
2561 936 594 360
RsrII CG'GwC_CG
Cuts at: 2646 2646
Size: 4451
SacI G_AGCT'C
Cuts at: 1465 1465
Size: 4451
SacII CC_GC'GG
Cuts at: 141 1393 141
Size: 1252 3199
SalI G'TCGA_C
Cuts at: 269 1488 269
Size: 1219 3232
SapI GCTCTTCn'nnn_
Cuts at: 2474 2684 2474
Size: 210 4241
SbfI CC_TGCA'GG
Cuts at: 1361 1498 1361
Size: 137 4314
ScaI AGT'ACT
Cuts at: 3968 3968
Size: 4451
SexAI A'CCwGG_T
Cuts at: 691 691
Size: 4451
SfcI C'TryA_G
Cuts at: 33 341 375 437 998 1357 1406 1494 2179
Size: 308 34 62 561 359 49 88 685
Cuts at: 2179 3172 3363 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 561 359 308 191 88
62 49 34
SfoI GGC'GCC
Cuts at: 303 546 715 746 1883 2131 303
Size: 243 169 31 1137 248 2623
Fragments arranged by size:
2623 1137 248 243 169 31
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4451
SgrAI Cr'CCGG_yG
Cuts at: 710 710
Size: 4451
SmaI CCC'GGG
Cuts at: 571 853 1473 571
Size: 282 620 3549
Fragments arranged by size:
3549 620 282
SmlI C'TyrA_G
Cuts at: 589 718 1004 1606 2521 3013 3275 589
Size: 129 286 602 915 492 262 1765
Fragments arranged by size:
1765 915 602 492 286 262 129
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4451
SphI G_CATG'C
Cuts at: 1504 2004 1504
Size: 500 3951
StuI AGG'CCT
Cuts at: 595 595
Size: 4451
StyI C'CwwG_G
Cuts at: 308 1117 1219 1585 1939 308
Size: 809 102 366 354 2820
Fragments arranged by size:
2820 809 366 354 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 694 2034 2167 2626 2835 694
Size: 1340 133 459 209 2310
Fragments arranged by size:
2310 1340 459 209 133
TatI w'GTAC_w
Cuts at: 583 3966 583
Size: 3383 1068
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3968 4000 3968
Size: 32 4419
Tth111I GACn'n_nGTC
Cuts at: 2248 2248
Size: 4451
XbaI T'CTAG_A
Cuts at: 1482 1482
Size: 4451
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 358 1426 1946 358
Size: 1068 520 2863
Fragments arranged by size:
2863 1068 520
XhoI C'TCGA_G
Cuts at: 1004 1004
Size: 4451
XmaI C'CCGG_G
Cuts at: 569 851 1471 569
Size: 282 620 3549
Fragments arranged by size:
3549 620 282
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BciVI
BclI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NheI NotI NspI PciI
PflMI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AflII AlfI AloI ApaI AscI BbvCI BcgI
BfrBI BglI BmgBI Bpu10I BsaXI BsiWI BsmI BsmBI
BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NruI NsiI PacI PasI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SanDI SfiI SnaBI SrfI SspI
SwaI XmnI ZraI