Restriction map for
pF1KB9728
(Circular) (Six-base) MAPSORT of: pF1KB9728.seq Check: 4767 from: 1 to: 4445
pF1KB9728 4445 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 1483 1483
Size: 4445
Acc65I G'GTAC_C
Cuts at: 1056 1461 1056
Size: 405 4040
AclI AA'CG_TT
Cuts at: 4285 4285
Size: 4445
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 678 2270 2702 3449 3717 678
Size: 1592 432 747 268 1406
Fragments arranged by size:
1592 1406 747 432 268
AflIII A'CryG_T
Cuts at: 932 1797 2901 3878 932
Size: 865 1104 977 1499
Fragments arranged by size:
1499 1104 977 865
AgeI A'CCGG_T
Cuts at: 2833 2833
Size: 4445
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1181 1181
Size: 4445
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 655 687 655
Size: 32 4413
AlwNI CAG_nnn'CTG
Cuts at: 495 1750 3317 495
Size: 1255 1567 1623
Fragments arranged by size:
1623 1567 1255
ApaLI G'TGCA_C
Cuts at: 3215 3215
Size: 4445
ApoI r'AATT_y
Cuts at: 1449 3798 4054 1449
Size: 2349 256 1840
Fragments arranged by size:
2349 1840 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4445
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4445
AvaI C'yCGr_G
Cuts at: 384 400 601 945 988 1465 384
Size: 16 201 344 43 477 3364
Fragments arranged by size:
3364 477 344 201 43 16
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1048 1081 1048
Size: 33 4412
BamHI G'GATC_C
Cuts at: 1470 1470
Size: 4445
BanI G'GyrC_C
Cuts at: 352 367 523 792 804 880 975 1056 1138
Size: 15 156 269 12 76 95 81 82
Cuts at: 1138 1461 1875 2123 2158 352
Size: 323 414 248 35 2639
Fragments arranged by size:
2639 414 323 269 248 156 95 82
81 76 35 15 12
BanII G_rGCy'C
Cuts at: 196 385 454 670 1114 1132 1459 2489 196
Size: 189 69 216 444 18 327 1030 2152
Fragments arranged by size:
2152 1030 444 327 216 189 69 18
BbeI G_GCGC'C
Cuts at: 356 371 527 796 808 884 1142 1879 2127
Size: 15 156 269 12 76 258 737 248
Cuts at: 2127 356
Size: 2674
Fragments arranged by size:
2674 737 269 258 248 156 76 15
12
BbvCI CC'TCA_GC
Cuts at: 1371 1371
Size: 4445
BciVI GTATCCnnnnn_n'
Cuts at: 2343 3104 4445 2343
Size: 761 1341 2343
Fragments arranged by size:
2343 1341 761
BclI T'GATC_A
Cuts at: 1965 1965
Size: 4445
BglI GCCn_nnn'nGGC
Cuts at: 316 376 830 1198 316
Size: 60 454 368 3563
Fragments arranged by size:
3563 454 368 60
BglII A'GATC_T
Cuts at: 482 1960 3997 482
Size: 1478 2037 930
Fragments arranged by size:
2037 1478 930
BlpI GC'TnA_GC
Cuts at: 475 1557 475
Size: 1082 3363
Bme1580I G_kGCm'C
Cuts at: 980 2070 2163 3219 980
Size: 1090 93 1056 2206
Fragments arranged by size:
2206 1090 1056 93
BmgBI CAC'GTC
Cuts at: 924 924
Size: 4445
BmrI ACTGGGnnnn_n'
Cuts at: 1657 1738 2073 4217 1657
Size: 81 335 2144 1885
Fragments arranged by size:
2144 1885 335 81
BmtI G_CTAG'C
Cuts at: 1379 1379
Size: 4445
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 258 684 1290 2812 258
Size: 426 606 1522 1891
Fragments arranged by size:
1891 1522 606 426
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 1146 1197 1255 1295 160
Size: 986 51 58 40 3310
Fragments arranged by size:
3310 986 58 51 40
Bpu10I CC'TnA_GC
Cuts at: 1371 1371
Size: 4445
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 1138 1621 2500 2992 3290 700
Size: 438 483 879 492 298 1855
Fragments arranged by size:
1855 879 492 483 438 298
BsaI GGTCTCn'nnnn_
Cuts at: 229 4149 229
Size: 3920 525
BsaAI yAC'GTr
Cuts at: 1798 1798
Size: 4445
BsaBI GATnn'nnATC
Cuts at: 6 1959 3996 6
Size: 1953 2037 455
Fragments arranged by size:
2037 1953 455
BsaHI Gr'CG_yC
Cuts at: 353 368 524 760 793 805 869 881 995
Size: 15 156 236 33 12 64 12 114
Cuts at: 995 1139 1174 1876 2124 2826 4322 353
Size: 144 35 702 248 702 1496 476
Fragments arranged by size:
1496 702 702 476 248 236 156 144
114 64 35 33 15 12 12
BsaWI w'CCGG_w
Cuts at: 1634 2155 2833 3107 3254 3665 1634
Size: 521 678 274 147 411 2414
Fragments arranged by size:
2414 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 655 685 655
Size: 30 4415
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 234 237 240 282 676 234
Size: 3 3 42 394 4003
Fragments arranged by size:
4003 394 42 3 3
BseYI C'CCAG_C
Cuts at: 457 886 1177 1870 2628 3205 3701 457
Size: 429 291 693 758 577 496 1201
Fragments arranged by size:
1201 758 693 577 496 429 291
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 1192 174
Size: 1018 3427
BsiEI CG_ry'CG
Cuts at: 104 685 1007 1987 2033 3241 104
Size: 581 322 980 46 1208 1308
Fragments arranged by size:
1308 1208 980 581 322 46
BsiHKAI G_wGCw'C
Cuts at: 385 454 1132 1459 2237 2427 3219 385
Size: 69 678 327 778 190 792 1611
Fragments arranged by size:
1611 792 778 678 327 190 69
BsmI GAATG_Cn'
Cuts at: 1096 1096
Size: 4445
Bsp1286I G_dGCh'C
Cuts at: 196 385 454 670 980 1114 1132 1459 2070
Size: 189 69 216 310 134 18 327 611
Cuts at: 2070 2163 2237 2427 2489 3219 196
Size: 93 74 190 62 730 1422
Fragments arranged by size:
1422 730 611 327 310 216 190 189
134 93 74 69 62 18
BspEI T'CCGG_A
Cuts at: 3665 3665
Size: 4445
BspHI T'CATG_A
Cuts at: 3621 3621
Size: 4445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1495 1709 2011 2392 1495
Size: 214 302 381 3548
Fragments arranged by size:
3548 381 302 214
BsrBI CCG'CTC
Cuts at: 50 141 362 743 2737 2791 4275 4440 50
Size: 91 221 381 1994 54 1484 165 55
Fragments arranged by size:
1994 1484 381 221 165 91 55 54
BsrDI GCAATG_nn'
Cuts at: 2357 2357
Size: 4445
BsrFI r'CCGG_y
Cuts at: 132 680 871 2443 2624 2833 132
Size: 548 191 1572 181 209 1744
Fragments arranged by size:
1744 1572 548 209 191 181
BssHII G'CGCG_C
Cuts at: 170 502 170
Size: 332 4113
BssSI C'ACGA_G
Cuts at: 1107 2716 3074 1107
Size: 1609 358 2478
Fragments arranged by size:
2478 1609 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1822 1822
Size: 4445
BstBI TT'CG_AA
Cuts at: 2806 2806
Size: 4445
BstXI CCAn_nnnn'nTGG
Cuts at: 960 960
Size: 4445
BstYI r'GATC_y
Cuts at: 1 482 1470 1937 1960 2295 2541 3553 3639
Size: 481 988 467 23 335 246 1012 86
Cuts at: 3639 3651 3997 1
Size: 12 346 449
Fragments arranged by size:
1012 988 481 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 593 593
Size: 4445
BtgI C'CryG_G
Cuts at: 519 857 980 1073 519
Size: 338 123 93 3891
Fragments arranged by size:
3891 338 123 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 408 2395 2576 2739 408
Size: 1987 181 163 2114
Fragments arranged by size:
2114 1987 181 163
BtsI GCAGTG_nn'
Cuts at: 1688 1688
Size: 4445
DraI TTT'AAA
Cuts at: 1444 1444
Size: 4445
DraIII CAC_nnn'GTG
Cuts at: 735 735
Size: 4445
DrdI GACnn_nn'nnGTC
Cuts at: 2151 3009 2151
Size: 858 3587
EaeI y'GGCC_r
Cuts at: 302 440 641 682 962 1004 1070 2030 2204
Size: 138 201 41 280 42 66 960 174
Cuts at: 2204 2595 2622 3967 302
Size: 391 27 1345 780
Fragments arranged by size:
1345 960 780 391 280 201 174 138
66 42 41 27
EagI C'GGCC_G
Cuts at: 682 1004 2030 682
Size: 322 1026 3097
Fragments arranged by size:
3097 1026 322
EarI CTCTTCn'nnn_
Cuts at: 2468 2678 2468
Size: 210 4235
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 133 255 664 714 2653 2963 3109 4255 133
Size: 122 409 50 1939 310 146 1146 323
Fragments arranged by size:
1939 1146 409 323 310 146 122 50
EcoICRI GAG'CTC
Cuts at: 383 452 1130 1457 383
Size: 69 678 327 3371
Fragments arranged by size:
3371 678 327 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 678 1146 1197 1255 1295 2270 2702 3449
Size: 518 468 51 58 40 975 432 747
Cuts at: 3449 3717 160
Size: 268 888
Fragments arranged by size:
975 888 747 518 468 432 268 58
51 40
EcoO109I rG'GnC_Cy
Cuts at: 597 985 1269 1584 597
Size: 388 284 315 3458
Fragments arranged by size:
3458 388 315 284
EcoRI G'AATT_C
Cuts at: 1449 1449
Size: 4445
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2464 2464
Size: 4445
FspI TGC'GCA
Cuts at: 2226 2226
Size: 4445
HaeII r_GCGC'y
Cuts at: 311 356 371 468 527 715 796 808 884
Size: 45 15 97 59 188 81 12 76
Cuts at: 884 1085 1142 1879 2127 3149 4128 311
Size: 201 57 737 248 1022 979 628
Fragments arranged by size:
1022 979 737 628 248 201 188 97
81 76 59 57 45 15 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1398 1430 2287 2317 2319 2349 1398
Size: 32 857 30 2 30 3494
Fragments arranged by size:
3494 857 32 30 30 2
HincII GTy'rAC
Cuts at: 1484 1484
Size: 4445
KasI G'GCGC_C
Cuts at: 352 367 523 792 804 880 1138 1875 2123
Size: 15 156 269 12 76 258 737 248
Cuts at: 2123 352
Size: 2674
Fragments arranged by size:
2674 737 269 258 248 156 76 15
12
KpnI G_GTAC'C
Cuts at: 1060 1465 1060
Size: 405 4040
MluI A'CGCG_T
Cuts at: 3878 3878
Size: 4445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3116 3300 3116
Size: 184 4261
MscI TGG'CCA
Cuts at: 442 964 2206 442
Size: 522 1242 2681
Fragments arranged by size:
2681 1242 522
MslI CAynn'nnrTG
Cuts at: 436 958 2434 2561 436
Size: 522 1476 127 2320
Fragments arranged by size:
2320 1476 522 127
MspA1I CmG'CkG
Cuts at: 521 859 968 982 1556 1870 2230 3243 3488
Size: 338 109 14 574 314 360 1013 245
Cuts at: 3488 521
Size: 1478
Fragments arranged by size:
1478 1013 574 360 338 314 245 109
14
NaeI GCC'GGC
Cuts at: 134 682 873 2626 134
Size: 548 191 1753 1953
Fragments arranged by size:
1953 1753 548 191
NarI GG'CG_CC
Cuts at: 353 368 524 793 805 881 1139 1876 2124
Size: 15 156 269 12 76 258 737 248
Cuts at: 2124 353
Size: 2674
Fragments arranged by size:
2674 737 269 258 248 156 76 15
12
NcoI C'CATG_G
Cuts at: 1073 1073
Size: 4445
NgoMIV G'CCGG_C
Cuts at: 132 680 871 2624 132
Size: 548 191 1753 1953
Fragments arranged by size:
1953 1753 548 191
NheI G'CTAG_C
Cuts at: 1375 1375
Size: 4445
NruI TCG'CGA
Cuts at: 1265 1265
Size: 4445
NspI r_CATG'y
Cuts at: 936 1221 1498 1998 2905 936
Size: 285 277 500 907 2476
Fragments arranged by size:
2476 907 500 285 277
PasI CC'CwG_GG
Cuts at: 774 774
Size: 4445
PciI A'CATG_T
Cuts at: 932 2901 932
Size: 1969 2476
PflMI CCAn_nnn'nTGG
Cuts at: 735 735
Size: 4445
PmeI GTTT'AAAC
Cuts at: 1444 1444
Size: 4445
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 640 655 672 687 3584 3616 640
Size: 15 17 15 2897 32 1469
Fragments arranged by size:
2897 1469 32 17 15 15
PpuMI rG'GwC_Cy
Cuts at: 985 1269 985
Size: 284 4161
PstI C_TGCA'G
Cuts at: 500 1492 2177 500
Size: 992 685 2768
Fragments arranged by size:
2768 992 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4445
PvuII CAG'CTG
Cuts at: 968 1870 2230 968
Size: 902 360 3183
Fragments arranged by size:
3183 902 360
RsrII CG'GwC_CG
Cuts at: 2640 2640
Size: 4445
SacI G_AGCT'C
Cuts at: 385 454 1132 1459 385
Size: 69 678 327 3371
Fragments arranged by size:
3371 678 327 69
SacII CC_GC'GG
Cuts at: 522 860 983 522
Size: 338 123 3984
Fragments arranged by size:
3984 338 123
SalI G'TCGA_C
Cuts at: 1482 1482
Size: 4445
SanDI GG'GwC_CC
Cuts at: 985 985
Size: 4445
SapI GCTCTTCn'nnn_
Cuts at: 2468 2678 2468
Size: 210 4235
SbfI CC_TGCA'GG
Cuts at: 500 1492 500
Size: 992 3453
ScaI AGT'ACT
Cuts at: 3962 3962
Size: 4445
SfcI C'TryA_G
Cuts at: 33 496 1488 2173 3166 3357 33
Size: 463 992 685 993 191 1121
Fragments arranged by size:
1121 993 992 685 463 191
SfoI GGC'GCC
Cuts at: 354 369 525 794 806 882 1140 1877 2125
Size: 15 156 269 12 76 258 737 248
Cuts at: 2125 354
Size: 2674
Fragments arranged by size:
2674 737 269 258 248 156 76 15
12
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4445
SgrAI Cr'CCGG_yG
Cuts at: 871 871
Size: 4445
SmaI CCC'GGG
Cuts at: 947 990 1467 947
Size: 43 477 3925
Fragments arranged by size:
3925 477 43
SmlI C'TyrA_G
Cuts at: 715 1153 1600 2515 3007 3269 715
Size: 438 447 915 492 262 1891
Fragments arranged by size:
1891 915 492 447 438 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4445
SphI G_CATG'C
Cuts at: 1498 1998 1498
Size: 500 3945
StuI AGG'CCT
Cuts at: 1205 1205
Size: 4445
StyI C'CwwG_G
Cuts at: 1073 1579 1933 1073
Size: 506 354 3585
Fragments arranged by size:
3585 506 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2028 2161 2620 2829 2028
Size: 133 459 209 3644
Fragments arranged by size:
3644 459 209 133
TatI w'GTAC_w
Cuts at: 3960 3960
Size: 4445
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3962 3994 3962
Size: 32 4413
Tth111I GACn'n_nGTC
Cuts at: 2242 2242
Size: 4445
XbaI T'CTAG_A
Cuts at: 1476 1476
Size: 4445
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1940 1940
Size: 4445
XmaI C'CCGG_G
Cuts at: 945 988 1465 945
Size: 43 477 3925
Fragments arranged by size:
3925 477 43
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NruI NspI PasI PciI PflMI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI ApaI AscI
AvrII BbsI BcgI BfrBI BsiWI BsmBI BsrGI BstEII
BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NotI NsiI PacI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SexAI SfiI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI