Restriction map for
pF1KB9727
(Circular) (Six-base) MAPSORT of: pF1KB9727.seq Check: 4699 from: 1 to: 4430
pF1KB9727 4430 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 790 790
Size: 4430
AccI GT'mk_AC
Cuts at: 1468 1468
Size: 4430
Acc65I G'GTAC_C
Cuts at: 1446 1446
Size: 4430
AclI AA'CG_TT
Cuts at: 4270 4270
Size: 4430
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 152 1004 2255 2687 3434 3702 152
Size: 852 1251 432 747 268 880
Fragments arranged by size:
1251 880 852 747 432 268
AflIII A'CryG_T
Cuts at: 1782 2886 3863 1782
Size: 1104 977 2349
Fragments arranged by size:
2349 1104 977
AgeI A'CCGG_T
Cuts at: 2818 2818
Size: 4430
AlwNI CAG_nnn'CTG
Cuts at: 1735 3302 1735
Size: 1567 2863
ApaLI G'TGCA_C
Cuts at: 3200 3200
Size: 4430
ApoI r'AATT_y
Cuts at: 1434 3783 4039 1434
Size: 2349 256 1825
Fragments arranged by size:
2349 1825 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4430
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4430
AvaI C'yCGr_G
Cuts at: 1450 1450
Size: 4430
BamHI G'GATC_C
Cuts at: 1455 1455
Size: 4430
BanI G'GyrC_C
Cuts at: 806 1446 1860 2108 2143 806
Size: 640 414 248 35 3093
Fragments arranged by size:
3093 640 414 248 35
BanII G_rGCy'C
Cuts at: 665 1444 2474 665
Size: 779 1030 2621
Fragments arranged by size:
2621 1030 779
BbeI G_GCGC'C
Cuts at: 1864 2112 1864
Size: 248 4182
BbsI GAAGACnn'nnnn_
Cuts at: 854 854
Size: 4430
BciVI GTATCCnnnnn_n'
Cuts at: 2328 3089 4430 2328
Size: 761 1341 2328
Fragments arranged by size:
2328 1341 761
BclI T'GATC_A
Cuts at: 218 1950 218
Size: 1732 2698
BfrBI ATG'CAT
Cuts at: 673 673
Size: 4430
BglII A'GATC_T
Cuts at: 894 1945 3982 894
Size: 1051 2037 1342
Fragments arranged by size:
2037 1342 1051
BlpI GC'TnA_GC
Cuts at: 1542 1542
Size: 4430
Bme1580I G_kGCm'C
Cuts at: 809 2055 2148 3204 809
Size: 1246 93 1056 2035
Fragments arranged by size:
2035 1246 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1642 1723 2058 4202 1642
Size: 81 335 2144 1870
Fragments arranged by size:
2144 1870 335 81
BmtI G_CTAG'C
Cuts at: 794 794
Size: 4430
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1099 2797 1099
Size: 1698 2732
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 803 1124 274
Size: 529 321 3580
Fragments arranged by size:
3580 529 321
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 739 1606 2485 2977 3275 247
Size: 492 867 879 492 298 1402
Fragments arranged by size:
1402 879 867 492 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 445 1171 4134 445
Size: 726 2963 741
Fragments arranged by size:
2963 741 726
BsaAI yAC'GTr
Cuts at: 1783 1783
Size: 4430
BsaBI GATnn'nnATC
Cuts at: 6 899 1944 3981 6
Size: 893 1045 2037 455
Fragments arranged by size:
2037 1045 893 455
BsaHI Gr'CG_yC
Cuts at: 1861 2109 2811 4307 1861
Size: 248 702 1496 1984
Fragments arranged by size:
1984 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1619 2140 2818 3092 3239 3650 1619
Size: 521 678 274 147 411 2399
Fragments arranged by size:
2399 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 256 256
Size: 4430
BseYI C'CCAG_C
Cuts at: 741 1045 1855 2613 3190 3686 741
Size: 304 810 758 577 496 1485
Fragments arranged by size:
1485 810 758 577 496 304
BsiEI CG_ry'CG
Cuts at: 104 1972 2018 3226 104
Size: 1868 46 1208 1308
Fragments arranged by size:
1868 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1080 1444 2222 2412 3204 1080
Size: 364 778 190 792 2306
Fragments arranged by size:
2306 792 778 364 190
Bsp1286I G_dGCh'C
Cuts at: 665 809 1080 1444 2055 2148 2222 2412 2474
Size: 144 271 364 611 93 74 190 62
Cuts at: 2474 3204 665
Size: 730 1891
Fragments arranged by size:
1891 730 611 364 271 190 144 93
74 62
BspEI T'CCGG_A
Cuts at: 3650 3650
Size: 4430
BspHI T'CATG_A
Cuts at: 3606 3606
Size: 4430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 790 1480 1694 1996 2377 790
Size: 690 214 302 381 2843
Fragments arranged by size:
2843 690 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2722 2776 4260 4425 50
Size: 2672 54 1484 165 55
Fragments arranged by size:
2672 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 614 673 1397 2342 614
Size: 59 724 945 2702
Fragments arranged by size:
2702 945 724 59
BsrFI r'CCGG_y
Cuts at: 2428 2609 2818 2428
Size: 181 209 4040
Fragments arranged by size:
4040 209 181
BssSI C'ACGA_G
Cuts at: 1090 2701 3059 1090
Size: 1611 358 2461
Fragments arranged by size:
2461 1611 358
BstAPI GCAn_nnn'nTGC
Cuts at: 778 1807 778
Size: 1029 3401
BstBI TT'CG_AA
Cuts at: 2791 2791
Size: 4430
BstXI CCAn_nnnn'nTGG
Cuts at: 1296 1296
Size: 4430
BstYI r'GATC_y
Cuts at: 1 894 1455 1922 1945 2280 2526 3538 3624
Size: 893 561 467 23 335 246 1012 86
Cuts at: 3624 3636 3982 1
Size: 12 346 449
Fragments arranged by size:
1012 893 561 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 1112 107
Size: 1005 3425
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2380 2561 2724 2380
Size: 181 163 4086
Fragments arranged by size:
4086 181 163
BtsI GCAGTG_nn'
Cuts at: 1078 1167 1673 1078
Size: 89 506 3835
Fragments arranged by size:
3835 506 89
DraI TTT'AAA
Cuts at: 305 1429 305
Size: 1124 3306
DrdI GACnn_nn'nnGTC
Cuts at: 121 1185 1291 2136 2994 121
Size: 1064 106 845 858 1557
Fragments arranged by size:
1557 1064 858 845 106
EaeI y'GGCC_r
Cuts at: 1305 1339 2015 2189 2580 2607 3952 1305
Size: 34 676 174 391 27 1345 1783
Fragments arranged by size:
1783 1345 676 391 174 34 27
EagI C'GGCC_G
Cuts at: 2015 2015
Size: 4430
EarI CTCTTCn'nnn_
Cuts at: 587 1058 1235 1244 2453 2663 587
Size: 471 177 9 1209 210 2354
Fragments arranged by size:
2354 1209 471 210 177 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2638 2948 3094 4240 2638
Size: 310 146 1146 2828
Fragments arranged by size:
2828 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1442 1442
Size: 4430
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 152 274 803 1004 1124 2255 2687 3434 3702
Size: 122 529 201 120 1131 432 747 268
Cuts at: 3702 152
Size: 880
Fragments arranged by size:
1131 880 747 529 432 268 201 122
120
EcoNI CCTnn'n_nnAGG
Cuts at: 551 1420 551
Size: 869 3561
EcoO109I rG'GnC_Cy
Cuts at: 835 1413 1569 835
Size: 578 156 3696
Fragments arranged by size:
3696 578 156
EcoRI G'AATT_C
Cuts at: 1434 1434
Size: 4430
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 913 2449 913
Size: 1536 2894
FspI TGC'GCA
Cuts at: 2211 2211
Size: 4430
HaeII r_GCGC'y
Cuts at: 1864 2112 3134 4113 1864
Size: 248 1022 979 2181
Fragments arranged by size:
2181 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 586 618 2272 2302 2304 2334 586
Size: 32 1654 30 2 30 2682
Fragments arranged by size:
2682 1654 32 30 30 2
HincII GTy'rAC
Cuts at: 1469 1469
Size: 4430
KasI G'GCGC_C
Cuts at: 1860 2108 1860
Size: 248 4182
KpnI G_GTAC'C
Cuts at: 1450 1450
Size: 4430
MluI A'CGCG_T
Cuts at: 3863 3863
Size: 4430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3101 3285 3101
Size: 184 4246
MscI TGG'CCA
Cuts at: 2191 2191
Size: 4430
MslI CAynn'nnrTG
Cuts at: 877 1009 1294 2419 2546 877
Size: 132 285 1125 127 2761
Fragments arranged by size:
2761 1125 285 132 127
MspA1I CmG'CkG
Cuts at: 566 1135 1150 1219 1541 1855 2215 3228 3473
Size: 569 15 69 322 314 360 1013 245
Cuts at: 3473 566
Size: 1523
Fragments arranged by size:
1523 1013 569 360 322 314 245 69
15
NaeI GCC'GGC
Cuts at: 2611 2611
Size: 4430
NarI GG'CG_CC
Cuts at: 1861 2109 1861
Size: 248 4182
NcoI C'CATG_G
Cuts at: 107 1112 107
Size: 1005 3425
NdeI CA'TA_TG
Cuts at: 975 975
Size: 4430
NgoMIV G'CCGG_C
Cuts at: 2609 2609
Size: 4430
NheI G'CTAG_C
Cuts at: 790 790
Size: 4430
NsiI A_TGCA'T
Cuts at: 675 675
Size: 4430
NspI r_CATG'y
Cuts at: 199 1407 1483 1983 2890 199
Size: 1208 76 500 907 1739
Fragments arranged by size:
1739 1208 907 500 76
PciI A'CATG_T
Cuts at: 2886 2886
Size: 4430
PmeI GTTT'AAAC
Cuts at: 1429 1429
Size: 4430
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3569 3601 3569
Size: 32 4398
PpuMI rG'GwC_Cy
Cuts at: 835 1413 835
Size: 578 3852
PstI C_TGCA'G
Cuts at: 354 1477 2162 354
Size: 1123 685 2622
Fragments arranged by size:
2622 1123 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4430
PvuII CAG'CTG
Cuts at: 1135 1219 1855 2215 1135
Size: 84 636 360 3350
Fragments arranged by size:
3350 636 360 84
RsrII CG'GwC_CG
Cuts at: 2625 2625
Size: 4430
SacI G_AGCT'C
Cuts at: 1444 1444
Size: 4430
SalI G'TCGA_C
Cuts at: 1467 1467
Size: 4430
SapI GCTCTTCn'nnn_
Cuts at: 2453 2663 2453
Size: 210 4220
SbfI CC_TGCA'GG
Cuts at: 1477 1477
Size: 4430
ScaI AGT'ACT
Cuts at: 510 3947 510
Size: 3437 993
SfcI C'TryA_G
Cuts at: 33 350 501 1320 1473 2158 3151 3342 33
Size: 317 151 819 153 685 993 191 1121
Fragments arranged by size:
1121 993 819 685 317 191 153 151
SfoI GGC'GCC
Cuts at: 1862 2110 1862
Size: 248 4182
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4430
SmaI CCC'GGG
Cuts at: 1452 1452
Size: 4430
SmlI C'TyrA_G
Cuts at: 262 718 1585 2500 2992 3254 262
Size: 456 867 915 492 262 1438
Fragments arranged by size:
1438 915 867 492 456 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4430
SphI G_CATG'C
Cuts at: 199 1483 1983 199
Size: 1284 500 2646
Fragments arranged by size:
2646 1284 500
StuI AGG'CCT
Cuts at: 939 939
Size: 4430
StyI C'CwwG_G
Cuts at: 107 200 206 966 1112 1564 1918 107
Size: 93 6 760 146 452 354 2619
Fragments arranged by size:
2619 760 452 354 146 93 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 469 2013 2146 2605 2814 469
Size: 1544 133 459 209 2085
Fragments arranged by size:
2085 1544 459 209 133
TatI w'GTAC_w
Cuts at: 508 568 3945 508
Size: 60 3377 993
Fragments arranged by size:
3377 993 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 120 152 216 248 819 851 3947 3979 120
Size: 32 64 32 571 32 3096 32 571
Fragments arranged by size:
3096 571 571 64 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2227 2227
Size: 4430
XbaI T'CTAG_A
Cuts at: 1461 1461
Size: 4430
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 973 1296 1925 973
Size: 323 629 3478
Fragments arranged by size:
3478 629 323
XmaI C'CCGG_G
Cuts at: 1450 1450
Size: 4430
XmnI GAAnn'nnTTC
Cuts at: 896 896
Size: 4430
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NsiI NspI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI Bpu10I
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NotI NruI PacI PasI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI