Restriction map for
pF1KB9720
(Circular) (Six-base) MAPSORT of: pF1KB9720.seq Check: 6576 from: 1 to: 4325
pF1KB9720 4325 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 255 331 388 960 1092 255
Size: 76 57 572 132 3488
Fragments arranged by size:
3488 572 132 76 57
AccI GT'mk_AC
Cuts at: 998 1363 998
Size: 365 3960
Acc65I G'GTAC_C
Cuts at: 1341 1341
Size: 4325
AclI AA'CG_TT
Cuts at: 4165 4165
Size: 4325
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 723 2150 2582 3329 3597 723
Size: 1427 432 747 268 1451
Fragments arranged by size:
1451 1427 747 432 268
AfeI AGC'GCT
Cuts at: 1153 1153
Size: 4325
AflIII A'CryG_T
Cuts at: 710 1677 2781 3758 710
Size: 967 1104 977 1277
Fragments arranged by size:
1277 1104 977 967
AgeI A'CCGG_T
Cuts at: 2713 2713
Size: 4325
AlwNI CAG_nnn'CTG
Cuts at: 627 1630 3197 627
Size: 1003 1567 1755
Fragments arranged by size:
1755 1567 1003
ApaLI G'TGCA_C
Cuts at: 3095 3095
Size: 4325
ApoI r'AATT_y
Cuts at: 1329 3678 3934 1329
Size: 2349 256 1720
Fragments arranged by size:
2349 1720 256
AscI GG'CGCG_CC
Cuts at: 757 757
Size: 4325
AseI AT'TA_AT
Cuts at: 20 20
Size: 4325
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4325
AvaI C'yCGr_G
Cuts at: 534 546 591 778 1311 1345 534
Size: 12 45 187 533 34 3514
Fragments arranged by size:
3514 533 187 45 34 12
BamHI G'GATC_C
Cuts at: 1350 1350
Size: 4325
BanI G'GyrC_C
Cuts at: 217 517 876 1160 1341 1755 2003 2038 217
Size: 300 359 284 181 414 248 35 2504
Fragments arranged by size:
2504 414 359 300 284 248 181 35
BanII G_rGCy'C
Cuts at: 553 641 833 1339 2369 553
Size: 88 192 506 1030 2509
Fragments arranged by size:
2509 1030 506 192 88
BbeI G_GCGC'C
Cuts at: 880 1164 1759 2007 880
Size: 284 595 248 3198
Fragments arranged by size:
3198 595 284 248
BbsI GAAGACnn'nnnn_
Cuts at: 309 1249 309
Size: 940 3385
BbvCI CC'TCA_GC
Cuts at: 370 370
Size: 4325
BciVI GTATCCnnnnn_n'
Cuts at: 2223 2984 4325 2223
Size: 761 1341 2223
Fragments arranged by size:
2223 1341 761
BclI T'GATC_A
Cuts at: 1262 1845 1262
Size: 583 3742
BglI GCCn_nnn'nGGC
Cuts at: 143 548 143
Size: 405 3920
BglII A'GATC_T
Cuts at: 1840 3877 1840
Size: 2037 2288
BlpI GC'TnA_GC
Cuts at: 635 1437 635
Size: 802 3523
Bme1580I G_kGCm'C
Cuts at: 1950 2043 3099 1950
Size: 93 1056 3176
Fragments arranged by size:
3176 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1537 1618 1953 4097 1537
Size: 81 335 2144 1765
Fragments arranged by size:
2144 1765 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 216 2692 216
Size: 2476 1849
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 843 843
Size: 4325
Bpu10I CC'TnA_GC
Cuts at: 370 817 370
Size: 447 3878
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 1006 1501 2380 2872 3170 430
Size: 576 495 879 492 298 1585
Fragments arranged by size:
1585 879 576 495 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 575 4029 575
Size: 3454 871
BsaAI yAC'GTr
Cuts at: 1678 1678
Size: 4325
BsaBI GATnn'nnATC
Cuts at: 6 1839 3876 6
Size: 1833 2037 455
Fragments arranged by size:
2037 1833 455
BsaHI Gr'CG_yC
Cuts at: 230 877 1049 1161 1246 1756 2004 2706 4202
Size: 647 172 112 85 510 248 702 1496
Cuts at: 4202 230
Size: 353
Fragments arranged by size:
1496 702 647 510 353 248 172 112
85
BsaWI w'CCGG_w
Cuts at: 419 1514 2035 2713 2987 3134 3545 419
Size: 1095 521 678 274 147 411 1199
Fragments arranged by size:
1199 1095 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 262 522 262
Size: 260 4065
BseYI C'CCAG_C
Cuts at: 128 696 981 1142 1148 1214 1750 2508 3085
Size: 568 285 161 6 66 536 758 577
Cuts at: 3085 3581 128
Size: 496 872
Fragments arranged by size:
872 758 577 568 536 496 285 161
66 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 853 505
Size: 348 3977
BsiEI CG_ry'CG
Cuts at: 104 191 256 495 656 1007 1867 1913 3121
Size: 87 65 239 161 351 860 46 1208
Cuts at: 3121 104
Size: 1308
Fragments arranged by size:
1308 1208 860 351 239 161 87 65
46
BsiHKAI G_wGCw'C
Cuts at: 833 1339 2117 2307 3099 833
Size: 506 778 190 792 2059
Fragments arranged by size:
2059 792 778 506 190
BsmBI CGTCTCn'nnnn_
Cuts at: 448 448
Size: 4325
Bsp1286I G_dGCh'C
Cuts at: 553 641 833 1339 1950 2043 2117 2307 2369
Size: 88 192 506 611 93 74 190 62
Cuts at: 2369 3099 553
Size: 730 1779
Fragments arranged by size:
1779 730 611 506 192 190 93 88
74 62
BspEI T'CCGG_A
Cuts at: 3545 3545
Size: 4325
BspHI T'CATG_A
Cuts at: 3501 3501
Size: 4325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 255 331 388 960 984 1092 1375 1589 1891
Size: 76 57 572 24 108 283 214 302
Cuts at: 1891 2272 255
Size: 381 2308
Fragments arranged by size:
2308 572 381 302 283 214 108 76
57 24
BsrBI CCG'CTC
Cuts at: 50 252 299 575 2617 2671 4155 4320 50
Size: 202 47 276 2042 54 1484 165 55
Fragments arranged by size:
2042 1484 276 202 165 55 54 47
BsrDI GCAATG_nn'
Cuts at: 2237 2237
Size: 4325
BsrFI r'CCGG_y
Cuts at: 121 383 600 786 895 2323 2504 2713 121
Size: 262 217 186 109 1428 181 209 1733
Fragments arranged by size:
1733 1428 262 217 209 186 181 109
BssHII G'CGCG_C
Cuts at: 183 630 757 927 954 1188 183
Size: 447 127 170 27 234 3320
Fragments arranged by size:
3320 447 234 170 127 27
BssSI C'ACGA_G
Cuts at: 2596 2954 2596
Size: 358 3967
BstAPI GCAn_nnn'nTGC
Cuts at: 510 627 1702 510
Size: 117 1075 3133
Fragments arranged by size:
3133 1075 117
BstBI TT'CG_AA
Cuts at: 2686 2686
Size: 4325
BstEII G'GTnAC_C
Cuts at: 117 117
Size: 4325
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4325
BstYI r'GATC_y
Cuts at: 1 1350 1817 1840 2175 2421 3433 3519 3531
Size: 1349 467 23 335 246 1012 86 12
Cuts at: 3531 3877 1
Size: 346 449
Fragments arranged by size:
1349 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 113 521 746 932 107
Size: 6 408 225 186 3500
Fragments arranged by size:
3500 408 225 186 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1014 2275 2456 2619 1014
Size: 1261 181 163 2720
Fragments arranged by size:
2720 1261 181 163
BtsI GCAGTG_nn'
Cuts at: 572 1568 572
Size: 996 3329
DraI TTT'AAA
Cuts at: 1324 1324
Size: 4325
DrdI GACnn_nn'nnGTC
Cuts at: 2031 2889 2031
Size: 858 3467
EaeI y'GGCC_r
Cuts at: 110 188 253 492 524 653 791 887 917
Size: 78 65 239 32 129 138 96 30
Cuts at: 917 1004 1910 2084 2475 2502 3847 110
Size: 87 906 174 391 27 1345 588
Fragments arranged by size:
1345 906 588 391 239 174 138 129
96 87 78 65 32 30 27
EagI C'GGCC_G
Cuts at: 188 253 492 653 1004 1910 188
Size: 65 239 161 351 906 2603
Fragments arranged by size:
2603 906 351 239 161 65
EarI CTCTTCn'nnn_
Cuts at: 592 742 838 1042 2348 2558 592
Size: 150 96 204 1306 210 2359
Fragments arranged by size:
2359 1306 210 204 150 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 349 593 2533 2843 2989 4135 349
Size: 244 1940 310 146 1146 539
Fragments arranged by size:
1940 1146 539 310 244 146
EcoICRI GAG'CTC
Cuts at: 831 1337 831
Size: 506 3819
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 723 843 2150 2582 3329 3597 723
Size: 120 1307 432 747 268 1451
Fragments arranged by size:
1451 1307 747 432 268 120
EcoO109I rG'GnC_Cy
Cuts at: 1464 1464
Size: 4325
EcoRI G'AATT_C
Cuts at: 1329 1329
Size: 4325
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 471 2344 471
Size: 1873 2452
FspI TGC'GCA
Cuts at: 2106 2106
Size: 4325
HaeII r_GCGC'y
Cuts at: 559 808 862 880 1155 1164 1759 2007 3029
Size: 249 54 18 275 9 595 248 1022
Cuts at: 3029 4008 559
Size: 979 876
Fragments arranged by size:
1022 979 876 595 275 249 248 54
18 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 190 222 2167 2197 2199 2229 190
Size: 32 1945 30 2 30 2286
Fragments arranged by size:
2286 1945 32 30 30 2
HincII GTy'rAC
Cuts at: 999 1364 999
Size: 365 3960
KasI G'GCGC_C
Cuts at: 876 1160 1755 2003 876
Size: 284 595 248 3198
Fragments arranged by size:
3198 595 284 248
KpnI G_GTAC'C
Cuts at: 1345 1345
Size: 4325
MluI A'CGCG_T
Cuts at: 3758 3758
Size: 4325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 413 2996 3180 413
Size: 2583 184 1558
Fragments arranged by size:
2583 1558 184
MscI TGG'CCA
Cuts at: 112 2086 112
Size: 1974 2351
MslI CAynn'nnrTG
Cuts at: 112 311 2314 2441 112
Size: 199 2003 127 1996
Fragments arranged by size:
2003 1996 199 127
MspA1I CmG'CkG
Cuts at: 624 1218 1436 1750 2110 3123 3368 624
Size: 594 218 314 360 1013 245 1581
Fragments arranged by size:
1581 1013 594 360 314 245 218
NaeI GCC'GGC
Cuts at: 897 2506 897
Size: 1609 2716
NarI GG'CG_CC
Cuts at: 877 1161 1756 2004 877
Size: 284 595 248 3198
Fragments arranged by size:
3198 595 284 248
NcoI C'CATG_G
Cuts at: 107 113 107
Size: 6 4319
NgoMIV G'CCGG_C
Cuts at: 895 2504 895
Size: 1609 2716
NotI GC'GGCC_GC
Cuts at: 492 492
Size: 4325
NspI r_CATG'y
Cuts at: 1275 1378 1878 2785 1275
Size: 103 500 907 2815
Fragments arranged by size:
2815 907 500 103
PciI A'CATG_T
Cuts at: 2781 2781
Size: 4325
PfoI T'CCnGG_A
Cuts at: 962 962
Size: 4325
PmeI GTTT'AAAC
Cuts at: 1324 1324
Size: 4325
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3464 3496 3464
Size: 32 4293
PstI C_TGCA'G
Cuts at: 266 995 1091 1372 2057 266
Size: 729 96 281 685 2534
Fragments arranged by size:
2534 729 685 281 96
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4325
PvuII CAG'CTG
Cuts at: 624 1218 1750 2110 624
Size: 594 532 360 2839
Fragments arranged by size:
2839 594 532 360
RsrII CG'GwC_CG
Cuts at: 2520 2520
Size: 4325
SacI G_AGCT'C
Cuts at: 833 1339 833
Size: 506 3819
SalI G'TCGA_C
Cuts at: 997 1362 997
Size: 365 3960
SapI GCTCTTCn'nnn_
Cuts at: 742 838 1042 2348 2558 742
Size: 96 204 1306 210 2509
Fragments arranged by size:
2509 1306 210 204 96
SbfI CC_TGCA'GG
Cuts at: 995 1091 1372 995
Size: 96 281 3948
Fragments arranged by size:
3948 281 96
ScaI AGT'ACT
Cuts at: 436 3842 436
Size: 3406 919
SexAI A'CCwGG_T
Cuts at: 797 947 797
Size: 150 4175
SfcI C'TryA_G
Cuts at: 33 262 991 1087 1368 2053 3046 3237 33
Size: 229 729 96 281 685 993 191 1121
Fragments arranged by size:
1121 993 729 685 281 229 191 96
SfoI GGC'GCC
Cuts at: 878 1162 1757 2005 878
Size: 284 595 248 3198
Fragments arranged by size:
3198 595 284 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4325
SmaI CCC'GGG
Cuts at: 548 593 1347 548
Size: 45 754 3526
Fragments arranged by size:
3526 754 45
SmlI C'TyrA_G
Cuts at: 445 1021 1480 2395 2887 3149 445
Size: 576 459 915 492 262 1621
Fragments arranged by size:
1621 915 576 492 459 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4325
SphI G_CATG'C
Cuts at: 1378 1878 1378
Size: 500 3825
StyI C'CwwG_G
Cuts at: 107 113 1459 1813 107
Size: 6 1346 354 2619
Fragments arranged by size:
2619 1346 354 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1908 2041 2500 2709 1908
Size: 133 459 209 3524
Fragments arranged by size:
3524 459 209 133
TatI w'GTAC_w
Cuts at: 434 3840 434
Size: 3406 919
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3842 3874 3842
Size: 32 4293
Tth111I GACn'n_nGTC
Cuts at: 2122 2122
Size: 4325
XbaI T'CTAG_A
Cuts at: 1356 1356
Size: 4325
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1820 1820
Size: 4325
XmaI C'CCGG_G
Cuts at: 546 591 1345 546
Size: 45 754 3526
Fragments arranged by size:
3526 754 45
XmnI GAAnn'nnTTC
Cuts at: 454 454
Size: 4325
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsaXI BsiWI BsmI
BsrGI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NruI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI