Restriction map for
pF1KB9716
(Circular) (Six-base) MAPSORT of: pF1KB9716.seq Check: 3746 from: 1 to: 4292
pF1KB9716 4292 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AatII G_ACGT'C
Cuts at: 863 863
Size: 4292
AccI GT'mk_AC
Cuts at: 1330 1330
Size: 4292
Acc65I G'GTAC_C
Cuts at: 1308 1308
Size: 4292
AclI AA'CG_TT
Cuts at: 4132 4132
Size: 4292
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2117 2549 3296 3564 2117
Size: 432 747 268 2845
Fragments arranged by size:
2845 747 432 268
AflIII A'CryG_T
Cuts at: 989 1644 2748 3725 989
Size: 655 1104 977 1556
Fragments arranged by size:
1556 1104 977 655
AgeI A'CCGG_T
Cuts at: 2680 2680
Size: 4292
AleI CACnn'nnGTG
Cuts at: 158 245 293 158
Size: 87 48 4157
Fragments arranged by size:
4157 87 48
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1063 1063
Size: 4292
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 892 924 892
Size: 32 4260
AlwNI CAG_nnn'CTG
Cuts at: 816 1597 3164 816
Size: 781 1567 1944
Fragments arranged by size:
1944 1567 781
ApaLI G'TGCA_C
Cuts at: 3062 3062
Size: 4292
ApoI r'AATT_y
Cuts at: 1296 3645 3901 1296
Size: 2349 256 1687
Fragments arranged by size:
2349 1687 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4292
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4292
AvaI C'yCGr_G
Cuts at: 172 1241 1312 172
Size: 1069 71 3152
Fragments arranged by size:
3152 1069 71
BamHI G'GATC_C
Cuts at: 652 671 1317 652
Size: 19 646 3627
Fragments arranged by size:
3627 646 19
BanI G'GyrC_C
Cuts at: 1119 1308 1722 1970 2005 1119
Size: 189 414 248 35 3406
Fragments arranged by size:
3406 414 248 189 35
BanII G_rGCy'C
Cuts at: 736 1249 1306 2336 736
Size: 513 57 1030 2692
Fragments arranged by size:
2692 1030 513 57
BbeI G_GCGC'C
Cuts at: 1726 1974 1726
Size: 248 4044
BbsI GAAGACnn'nnnn_
Cuts at: 855 855
Size: 4292
BciVI GTATCCnnnnn_n'
Cuts at: 2190 2951 4292 2190
Size: 761 1341 2190
Fragments arranged by size:
2190 1341 761
BclI T'GATC_A
Cuts at: 1812 1812
Size: 4292
BglII A'GATC_T
Cuts at: 1807 3844 1807
Size: 2037 2255
BlpI GC'TnA_GC
Cuts at: 1404 1404
Size: 4292
Bme1580I G_kGCm'C
Cuts at: 494 1073 1917 2010 3066 494
Size: 579 844 93 1056 1720
Fragments arranged by size:
1720 1056 844 579 93
BmgBI CAC'GTC
Cuts at: 210 210
Size: 4292
BmrI ACTGGGnnnn_n'
Cuts at: 640 1155 1504 1585 1920 4064 640
Size: 515 349 81 335 2144 868
Fragments arranged by size:
2144 868 515 349 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 472 1061 2659 472
Size: 589 1598 2105
Fragments arranged by size:
2105 1598 589
Bpu10I CC'TnA_GC
Cuts at: 974 974
Size: 4292
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1468 2347 2839 3137 1468
Size: 879 492 298 2623
Fragments arranged by size:
2623 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 319 443 3996 319
Size: 124 3553 615
Fragments arranged by size:
3553 615 124
BsaAI yAC'GTr
Cuts at: 1068 1645 1068
Size: 577 3715
BsaBI GATnn'nnATC
Cuts at: 6 1806 3843 6
Size: 1800 2037 455
Fragments arranged by size:
2037 1800 455
BsaHI Gr'CG_yC
Cuts at: 379 860 1723 1971 2673 4169 379
Size: 481 863 248 702 1496 502
Fragments arranged by size:
1496 863 702 502 481 248
BsaWI w'CCGG_w
Cuts at: 521 1481 2002 2680 2954 3101 3512 521
Size: 960 521 678 274 147 411 1301
Fragments arranged by size:
1301 960 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 121 151 892 922 1084 1114 121
Size: 30 741 30 162 30 3299
Fragments arranged by size:
3299 741 162 30 30 30
BseYI C'CCAG_C
Cuts at: 493 565 604 806 915 978 1717 2475 3052
Size: 72 39 202 109 63 739 758 577
Cuts at: 3052 3548 493
Size: 496 1237
Fragments arranged by size:
1237 758 739 577 496 202 109 72
63 39
BsiEI CG_ry'CG
Cuts at: 104 195 1834 1880 3088 104
Size: 91 1639 46 1208 1308
Fragments arranged by size:
1639 1308 1208 91 46
BsiHKAI G_wGCw'C
Cuts at: 881 1042 1306 2084 2274 3066 881
Size: 161 264 778 190 792 2107
Fragments arranged by size:
2107 792 778 264 190 161
BsmBI CGTCTCn'nnnn_
Cuts at: 504 504
Size: 4292
Bsp1286I G_dGCh'C
Cuts at: 494 736 881 1042 1073 1249 1306 1917 2010
Size: 242 145 161 31 176 57 611 93
Cuts at: 2010 2084 2274 2336 3066 494
Size: 74 190 62 730 1720
Fragments arranged by size:
1720 730 611 242 190 176 161 145
93 74 62 57 31
BspEI T'CCGG_A
Cuts at: 521 3512 521
Size: 2991 1301
BspHI T'CATG_A
Cuts at: 3468 3468
Size: 4292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 305 1342 1556 1858 2239 305
Size: 1037 214 302 381 2358
Fragments arranged by size:
2358 1037 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2584 2638 4122 4287 50
Size: 2534 54 1484 165 55
Fragments arranged by size:
2534 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2204 2204
Size: 4292
BsrFI r'CCGG_y
Cuts at: 149 194 328 452 2290 2471 2680 149
Size: 45 134 124 1838 181 209 1761
Fragments arranged by size:
1838 1761 209 181 134 124 45
BssSI C'ACGA_G
Cuts at: 2563 2921 2563
Size: 358 3934
BstAPI GCAn_nnn'nTGC
Cuts at: 1669 1669
Size: 4292
BstBI TT'CG_AA
Cuts at: 2653 2653
Size: 4292
BstEII G'GTnAC_C
Cuts at: 1054 1054
Size: 4292
BstXI CCAn_nnnn'nTGG
Cuts at: 390 390
Size: 4292
BstYI r'GATC_y
Cuts at: 1 652 671 1317 1784 1807 2142 2388 3400
Size: 651 19 646 467 23 335 246 1012
Cuts at: 3400 3486 3498 3844 1
Size: 86 12 346 449
Fragments arranged by size:
1012 651 646 467 449 346 335 246
86 23 19 12
BtgI C'CryG_G
Cuts at: 107 288 107
Size: 181 4111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 157 2242 2423 2586 157
Size: 2085 181 163 1863
Fragments arranged by size:
2085 1863 181 163
BtsI GCAGTG_nn'
Cuts at: 117 1535 117
Size: 1418 2874
DraI TTT'AAA
Cuts at: 1291 1291
Size: 4292
DrdI GACnn_nn'nnGTC
Cuts at: 1998 2856 1998
Size: 858 3434
EaeI y'GGCC_r
Cuts at: 192 461 588 1080 1877 2051 2442 2469 3814
Size: 269 127 492 797 174 391 27 1345
Cuts at: 3814 192
Size: 670
Fragments arranged by size:
1345 797 670 492 391 269 174 127
27
EagI C'GGCC_G
Cuts at: 192 1877 192
Size: 1685 2607
EarI CTCTTCn'nnn_
Cuts at: 507 697 2315 2525 507
Size: 190 1618 210 2274
Fragments arranged by size:
2274 1618 210 190
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1239 2500 2810 2956 4102 1239
Size: 1261 310 146 1146 1429
Fragments arranged by size:
1429 1261 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1304 1304
Size: 4292
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2117 2549 3296 3564 2117
Size: 432 747 268 2845
Fragments arranged by size:
2845 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 463 463
Size: 4292
EcoO109I rG'GnC_Cy
Cuts at: 1431 1431
Size: 4292
EcoRI G'AATT_C
Cuts at: 1296 1296
Size: 4292
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1235 2311 1235
Size: 1076 3216
FseI GG_CCGG'CC
Cuts at: 198 198
Size: 4292
FspI TGC'GCA
Cuts at: 2073 2073
Size: 4292
HaeII r_GCGC'y
Cuts at: 635 1726 1974 2996 3975 635
Size: 1091 248 1022 979 952
Fragments arranged by size:
1091 1022 979 952 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 121 153 2134 2164 2166 2196 121
Size: 32 1981 30 2 30 2217
Fragments arranged by size:
2217 1981 32 30 30 2
HincII GTy'rAC
Cuts at: 1331 1331
Size: 4292
HindIII A'AGCT_T
Cuts at: 821 821
Size: 4292
KasI G'GCGC_C
Cuts at: 1722 1970 1722
Size: 248 4044
KpnI G_GTAC'C
Cuts at: 1312 1312
Size: 4292
MluI A'CGCG_T
Cuts at: 3725 3725
Size: 4292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2963 3147 2963
Size: 184 4108
MscI TGG'CCA
Cuts at: 590 1082 2053 590
Size: 492 971 2829
Fragments arranged by size:
2829 971 492
MslI CAynn'nnrTG
Cuts at: 112 158 245 293 2281 2408 112
Size: 46 87 48 1988 127 1996
Fragments arranged by size:
1996 1988 127 87 48 46
MspA1I CmG'CkG
Cuts at: 276 816 1261 1270 1403 1717 2077 3090 3335
Size: 540 445 9 133 314 360 1013 245
Cuts at: 3335 276
Size: 1233
Fragments arranged by size:
1233 1013 540 445 360 314 245 133
9
NaeI GCC'GGC
Cuts at: 196 2473 196
Size: 2277 2015
NarI GG'CG_CC
Cuts at: 1723 1971 1723
Size: 248 4044
NcoI C'CATG_G
Cuts at: 107 107
Size: 4292
NgoMIV G'CCGG_C
Cuts at: 194 2471 194
Size: 2277 2015
NspI r_CATG'y
Cuts at: 1345 1845 2752 1345
Size: 500 907 2885
Fragments arranged by size:
2885 907 500
PasI CC'CwG_GG
Cuts at: 928 928
Size: 4292
PciI A'CATG_T
Cuts at: 2748 2748
Size: 4292
PmeI GTTT'AAAC
Cuts at: 1291 1291
Size: 4292
PmlI CAC'GTG
Cuts at: 1068 1068
Size: 4292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 892 924 3431 3463 892
Size: 32 2507 32 1721
Fragments arranged by size:
2507 1721 32 32
PstI C_TGCA'G
Cuts at: 1339 2024 1339
Size: 685 3607
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4292
PvuII CAG'CTG
Cuts at: 276 816 1717 2077 276
Size: 540 901 360 2491
Fragments arranged by size:
2491 901 540 360
RsrII CG'GwC_CG
Cuts at: 2487 2487
Size: 4292
SacI G_AGCT'C
Cuts at: 1306 1306
Size: 4292
SalI G'TCGA_C
Cuts at: 1329 1329
Size: 4292
SapI GCTCTTCn'nnn_
Cuts at: 2315 2525 2315
Size: 210 4082
SbfI CC_TGCA'GG
Cuts at: 1339 1339
Size: 4292
ScaI AGT'ACT
Cuts at: 907 3809 907
Size: 2902 1390
SfcI C'TryA_G
Cuts at: 33 1335 2020 3013 3204 33
Size: 1302 685 993 191 1121
Fragments arranged by size:
1302 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1724 1972 1724
Size: 248 4044
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4292
SmaI CCC'GGG
Cuts at: 1243 1314 1243
Size: 71 4221
SmlI C'TyrA_G
Cuts at: 1447 2362 2854 3116 1447
Size: 915 492 262 2623
Fragments arranged by size:
2623 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4292
SphI G_CATG'C
Cuts at: 1345 1845 1345
Size: 500 3792
StyI C'CwwG_G
Cuts at: 107 1426 1780 107
Size: 1319 354 2619
Fragments arranged by size:
2619 1319 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 563 820 1875 2008 2467 2676 563
Size: 257 1055 133 459 209 2179
Fragments arranged by size:
2179 1055 459 257 209 133
TatI w'GTAC_w
Cuts at: 905 1163 3807 905
Size: 258 2644 1390
Fragments arranged by size:
2644 1390 258
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 585 617 3809 3841 585
Size: 32 3192 32 1036
Fragments arranged by size:
3192 1036 32 32
Tth111I GACn'n_nGTC
Cuts at: 2089 2089
Size: 4292
XbaI T'CTAG_A
Cuts at: 1323 1323
Size: 4292
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 369 645 975 1104 1787 369
Size: 276 330 129 683 2874
Fragments arranged by size:
2874 683 330 276 129
XmaI C'CCGG_G
Cuts at: 1241 1312 1241
Size: 71 4221
ZraI GAC'GTC
Cuts at: 861 861
Size: 4292
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PmeI PmlI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmtI BpmI BseRI BsgI
BsiWI BsmI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PfoI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI