Restriction map for
pF1KB9714
(Circular) (Six-base) MAPSORT of: pF1KB9714.seq Check: 5809 from: 1 to: 4259
pF1KB9714 4259 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 331 331
Size: 4259
AccI GT'mk_AC
Cuts at: 449 1025 1297 449
Size: 576 272 3411
Fragments arranged by size:
3411 576 272
Acc65I G'GTAC_C
Cuts at: 1275 1275
Size: 4259
AclI AA'CG_TT
Cuts at: 4099 4099
Size: 4259
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 538 2084 2516 3263 3531 478
Size: 60 1546 432 747 268 1206
Fragments arranged by size:
1546 1206 747 432 268 60
AflIII A'CryG_T
Cuts at: 1611 2715 3692 1611
Size: 1104 977 2178
Fragments arranged by size:
2178 1104 977
AgeI A'CCGG_T
Cuts at: 2647 2647
Size: 4259
AlwNI CAG_nnn'CTG
Cuts at: 269 915 1005 1180 1564 3131 269
Size: 646 90 175 384 1567 1397
Fragments arranged by size:
1567 1397 646 384 175 90
ApaI G_GGCC'C
Cuts at: 438 438
Size: 4259
ApaLI G'TGCA_C
Cuts at: 3029 3029
Size: 4259
ApoI r'AATT_y
Cuts at: 1236 1263 3612 3868 1236
Size: 27 2349 256 1627
Fragments arranged by size:
2349 1627 256 27
AseI AT'TA_AT
Cuts at: 20 20
Size: 4259
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4259
AvaI C'yCGr_G
Cuts at: 1279 1279
Size: 4259
BamHI G'GATC_C
Cuts at: 1284 1284
Size: 4259
BanI G'GyrC_C
Cuts at: 1096 1275 1689 1937 1972 1096
Size: 179 414 248 35 3383
Fragments arranged by size:
3383 414 248 179 35
BanII G_rGCy'C
Cuts at: 199 311 438 586 922 1106 1273 2303 199
Size: 112 127 148 336 184 167 1030 2155
Fragments arranged by size:
2155 1030 336 184 167 148 127 112
BbeI G_GCGC'C
Cuts at: 1693 1941 1693
Size: 248 4011
BbvCI CC'TCA_GC
Cuts at: 665 665
Size: 4259
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4225
BciVI GTATCCnnnnn_n'
Cuts at: 765 2157 2918 4259 765
Size: 1392 761 1341 765
Fragments arranged by size:
1392 1341 765 761
BclI T'GATC_A
Cuts at: 1779 1779
Size: 4259
BglI GCCn_nnn'nGGC
Cuts at: 646 646
Size: 4259
BglII A'GATC_T
Cuts at: 1774 3811 1774
Size: 2037 2222
BlpI GC'TnA_GC
Cuts at: 571 916 1371 571
Size: 345 455 3459
Fragments arranged by size:
3459 455 345
Bme1580I G_kGCm'C
Cuts at: 438 1884 1977 3033 438
Size: 1446 93 1056 1664
Fragments arranged by size:
1664 1446 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1471 1552 1887 4031 1471
Size: 81 335 2144 1699
Fragments arranged by size:
2144 1699 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 803 941 2626 803
Size: 138 1685 2436
Fragments arranged by size:
2436 1685 138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 1094 334
Size: 760 3499
Bpu10I CC'TnA_GC
Cuts at: 457 665 457
Size: 208 4051
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1435 2314 2806 3104 1435
Size: 879 492 298 2590
Fragments arranged by size:
2590 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 310 1057 1070 3963 310
Size: 747 13 2893 606
Fragments arranged by size:
2893 747 606 13
BsaAI yAC'GTr
Cuts at: 1612 1612
Size: 4259
BsaBI GATnn'nnATC
Cuts at: 6 1773 3810 6
Size: 1767 2037 455
Fragments arranged by size:
2037 1767 455
BsaHI Gr'CG_yC
Cuts at: 1690 1938 2640 4136 1690
Size: 248 702 1496 1813
Fragments arranged by size:
1813 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1448 1969 2647 2921 3068 3479 1448
Size: 521 678 274 147 411 2228
Fragments arranged by size:
2228 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 986 1016 1120 1150 986
Size: 30 104 30 4095
Fragments arranged by size:
4095 104 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 145 162 145
Size: 17 4242
BseYI C'CCAG_C
Cuts at: 180 585 949 1180 1684 2442 3019 3515 180
Size: 405 364 231 504 758 577 496 924
Fragments arranged by size:
924 758 577 504 496 405 364 231
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 307
Size: 4259
BsiEI CG_ry'CG
Cuts at: 104 152 1801 1847 3055 104
Size: 48 1649 46 1208 1308
Fragments arranged by size:
1649 1308 1208 48 46
BsiHKAI G_wGCw'C
Cuts at: 922 1273 2051 2241 3033 922
Size: 351 778 190 792 2148
Fragments arranged by size:
2148 792 778 351 190
BsmI GAATG_Cn'
Cuts at: 733 733
Size: 4259
Bsp1286I G_dGCh'C
Cuts at: 199 311 438 586 922 1106 1273 1884 1977
Size: 112 127 148 336 184 167 611 93
Cuts at: 1977 2051 2241 2303 3033 199
Size: 74 190 62 730 1425
Fragments arranged by size:
1425 730 611 336 190 184 167 148
127 112 93 74 62
BspEI T'CCGG_A
Cuts at: 3479 3479
Size: 4259
BspHI T'CATG_A
Cuts at: 965 3435 965
Size: 2470 1789
BspMI ACCTGCnnnn'nnnn_
Cuts at: 331 1309 1523 1825 2206 331
Size: 978 214 302 381 2384
Fragments arranged by size:
2384 978 381 302 214
BsrBI CCG'CTC
Cuts at: 50 190 561 2551 2605 4089 4254 50
Size: 140 371 1990 54 1484 165 55
Fragments arranged by size:
1990 1484 371 165 140 55 54
BsrDI GCAATG_nn'
Cuts at: 2171 2171
Size: 4259
BsrFI r'CCGG_y
Cuts at: 2257 2438 2647 2257
Size: 181 209 3869
Fragments arranged by size:
3869 209 181
BssSI C'ACGA_G
Cuts at: 2530 2888 2530
Size: 358 3901
BstAPI GCAn_nnn'nTGC
Cuts at: 285 1636 285
Size: 1351 2908
BstBI TT'CG_AA
Cuts at: 2620 2620
Size: 4259
BstXI CCAn_nnnn'nTGG
Cuts at: 1072 1072
Size: 4259
BstYI r'GATC_y
Cuts at: 1 1035 1284 1751 1774 2109 2355 3367 3453
Size: 1034 249 467 23 335 246 1012 86
Cuts at: 3453 3465 3811 1
Size: 12 346 449
Fragments arranged by size:
1034 1012 467 449 346 335 249 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 450 450
Size: 4259
BtgI C'CryG_G
Cuts at: 642 642
Size: 4259
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 393 2209 2390 2553 393
Size: 1816 181 163 2099
Fragments arranged by size:
2099 1816 181 163
BtsI GCAGTG_nn'
Cuts at: 286 295 1502 286
Size: 9 1207 3043
Fragments arranged by size:
3043 1207 9
DraI TTT'AAA
Cuts at: 1258 1258
Size: 4259
DrdI GACnn_nn'nnGTC
Cuts at: 1965 2823 1965
Size: 858 3401
EaeI y'GGCC_r
Cuts at: 866 1007 1844 2018 2409 2436 3781 866
Size: 141 837 174 391 27 1345 1344
Fragments arranged by size:
1345 1344 837 391 174 141 27
EagI C'GGCC_G
Cuts at: 1844 1844
Size: 4259
EarI CTCTTCn'nnn_
Cuts at: 436 2282 2492 436
Size: 1846 210 2203
Fragments arranged by size:
2203 1846 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2467 2777 2923 4069 2467
Size: 310 146 1146 2657
Fragments arranged by size:
2657 1146 310 146
EcoICRI GAG'CTC
Cuts at: 920 1271 920
Size: 351 3908
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 478 538 1094 2084 2516 3263 3531 334
Size: 144 60 556 990 432 747 268 1062
Fragments arranged by size:
1062 990 747 556 432 268 144 60
EcoNI CCTnn'n_nnAGG
Cuts at: 520 520
Size: 4259
EcoO109I rG'GnC_Cy
Cuts at: 1398 1398
Size: 4259
EcoRI G'AATT_C
Cuts at: 1236 1263 1236
Size: 27 4232
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2278 2278
Size: 4259
FspI TGC'GCA
Cuts at: 2040 2040
Size: 4259
HaeII r_GCGC'y
Cuts at: 690 1693 1941 2963 3942 690
Size: 1003 248 1022 979 1007
Fragments arranged by size:
1022 1007 1003 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1037 1069 1120 1152 2101 2131 2133 2163 1037
Size: 32 51 32 949 30 2 30 3133
Fragments arranged by size:
3133 949 51 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1298 1298
Size: 4259
KasI G'GCGC_C
Cuts at: 1689 1937 1689
Size: 248 4011
KpnI G_GTAC'C
Cuts at: 1279 1279
Size: 4259
MluI A'CGCG_T
Cuts at: 3692 3692
Size: 4259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1068 2930 3114 1068
Size: 1862 184 2213
Fragments arranged by size:
2213 1862 184
MscI TGG'CCA
Cuts at: 868 1009 2020 868
Size: 141 1011 3107
Fragments arranged by size:
3107 1011 141
MslI CAynn'nnrTG
Cuts at: 1091 2248 2375 1091
Size: 1157 127 2975
Fragments arranged by size:
2975 1157 127
MspA1I CmG'CkG
Cuts at: 225 531 1370 1684 2044 3057 3302 225
Size: 306 839 314 360 1013 245 1182
Fragments arranged by size:
1182 1013 839 360 314 306 245
NaeI GCC'GGC
Cuts at: 2440 2440
Size: 4259
NarI GG'CG_CC
Cuts at: 1690 1938 1690
Size: 248 4011
NcoI C'CATG_G
Cuts at: 642 642
Size: 4259
NgoMIV G'CCGG_C
Cuts at: 2438 2438
Size: 4259
NspI r_CATG'y
Cuts at: 1086 1312 1812 2719 1086
Size: 226 500 907 2626
Fragments arranged by size:
2626 907 500 226
PciI A'CATG_T
Cuts at: 2715 2715
Size: 4259
PflMI CCAn_nnn'nTGG
Cuts at: 1119 1119
Size: 4259
PmeI GTTT'AAAC
Cuts at: 1258 1258
Size: 4259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3398 3430 3398
Size: 32 4227
PspOMI G'GGCC_C
Cuts at: 434 434
Size: 4259
PstI C_TGCA'G
Cuts at: 772 798 1193 1306 1991 772
Size: 26 395 113 685 3040
Fragments arranged by size:
3040 685 395 113 26
PvuI CG_AT'CG
Cuts at: 104 152 104
Size: 48 4211
PvuII CAG'CTG
Cuts at: 531 1684 2044 531
Size: 1153 360 2746
Fragments arranged by size:
2746 1153 360
RsrII CG'GwC_CG
Cuts at: 2454 2454
Size: 4259
SacI G_AGCT'C
Cuts at: 922 1273 922
Size: 351 3908
SalI G'TCGA_C
Cuts at: 1296 1296
Size: 4259
SapI GCTCTTCn'nnn_
Cuts at: 2282 2492 2282
Size: 210 4049
SbfI CC_TGCA'GG
Cuts at: 772 1306 772
Size: 534 3725
ScaI AGT'ACT
Cuts at: 3776 3776
Size: 4259
SfcI C'TryA_G
Cuts at: 33 768 794 1026 1189 1302 1987 2980 3171
Size: 735 26 232 163 113 685 993 191
Cuts at: 3171 33
Size: 1121
Fragments arranged by size:
1121 993 735 685 232 191 163 113
26
SfoI GGC'GCC
Cuts at: 1691 1939 1691
Size: 248 4011
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4259
SmaI CCC'GGG
Cuts at: 1281 1281
Size: 4259
SmlI C'TyrA_G
Cuts at: 1414 2329 2821 3083 1414
Size: 915 492 262 2590
Fragments arranged by size:
2590 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4259
SphI G_CATG'C
Cuts at: 1312 1812 1312
Size: 500 3759
StuI AGG'CCT
Cuts at: 742 742
Size: 4259
StyI C'CwwG_G
Cuts at: 642 1393 1747 642
Size: 751 354 3154
Fragments arranged by size:
3154 751 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 922 1141 1842 1975 2434 2643 922
Size: 219 701 133 459 209 2538
Fragments arranged by size:
2538 701 459 219 209 133
TatI w'GTAC_w
Cuts at: 347 1223 3774 347
Size: 876 2551 832
Fragments arranged by size:
2551 876 832
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 986 1018 3776 3808 986
Size: 32 2758 32 1437
Fragments arranged by size:
2758 1437 32 32
Tth111I GACn'n_nGTC
Cuts at: 1049 2056 1049
Size: 1007 3252
XbaI T'CTAG_A
Cuts at: 1290 1290
Size: 4259
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1754 1754
Size: 4259
XmaI C'CCGG_G
Cuts at: 1279 1279
Size: 4259
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BfrBI BmgBI BmtI BsiWI BsmBI
BsrGI BssHII BstEII Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI