Restriction map for
pF1KB9700
(Circular) (Six-base) MAPSORT of: pF1KB9700.seq Check: 1366 from: 1 to: 4001
pF1KB9700 4001 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1039 1039
Size: 4001
Acc65I G'GTAC_C
Cuts at: 1017 1017
Size: 4001
AclI AA'CG_TT
Cuts at: 3841 3841
Size: 4001
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 600 680 768 936 1826 2258 3005 3273 600
Size: 80 88 168 890 432 747 268 1328
Fragments arranged by size:
1328 890 747 432 268 168 88 80
AflIII A'CryG_T
Cuts at: 844 1353 2457 3434 844
Size: 509 1104 977 1411
Fragments arranged by size:
1411 1104 977 509
AgeI A'CCGG_T
Cuts at: 2389 2389
Size: 4001
AleI CACnn'nnGTG
Cuts at: 911 911
Size: 4001
AlwNI CAG_nnn'CTG
Cuts at: 1306 2873 1306
Size: 1567 2434
ApaLI G'TGCA_C
Cuts at: 2771 2771
Size: 4001
ApoI r'AATT_y
Cuts at: 650 798 865 1005 3354 3610 650
Size: 148 67 140 2349 256 1041
Fragments arranged by size:
2349 1041 256 148 140 67
AseI AT'TA_AT
Cuts at: 20 20
Size: 4001
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4001
AvaI C'yCGr_G
Cuts at: 1021 1021
Size: 4001
BamHI G'GATC_C
Cuts at: 141 1026 141
Size: 885 3116
BanI G'GyrC_C
Cuts at: 482 1017 1431 1679 1714 482
Size: 535 414 248 35 2769
Fragments arranged by size:
2769 535 414 248 35
BanII G_rGCy'C
Cuts at: 1015 2045 1015
Size: 1030 2971
BbeI G_GCGC'C
Cuts at: 1435 1683 1435
Size: 248 3753
BciVI GTATCCnnnnn_n'
Cuts at: 288 1899 2660 4001 288
Size: 1611 761 1341 288
Fragments arranged by size:
1611 1341 761 288
BclI T'GATC_A
Cuts at: 1521 1521
Size: 4001
BglII A'GATC_T
Cuts at: 714 1516 3553 714
Size: 802 2037 1162
Fragments arranged by size:
2037 1162 802
BlpI GC'TnA_GC
Cuts at: 1113 1113
Size: 4001
Bme1580I G_kGCm'C
Cuts at: 1626 1719 2775 1626
Size: 93 1056 2852
Fragments arranged by size:
2852 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1213 1294 1629 3773 1213
Size: 81 335 2144 1441
Fragments arranged by size:
2144 1441 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 4001
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1177 2056 2548 2846 1177
Size: 879 492 298 2332
Fragments arranged by size:
2332 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3705 3705
Size: 4001
BsaAI yAC'GTr
Cuts at: 1354 1354
Size: 4001
BsaBI GATnn'nnATC
Cuts at: 6 1515 3552 6
Size: 1509 2037 455
Fragments arranged by size:
2037 1509 455
BsaHI Gr'CG_yC
Cuts at: 395 1432 1680 2382 3878 395
Size: 1037 248 702 1496 518
Fragments arranged by size:
1496 1037 702 518 248
BsaWI w'CCGG_w
Cuts at: 896 1190 1711 2389 2663 2810 3221 896
Size: 294 521 678 274 147 411 1676
Fragments arranged by size:
1676 678 521 411 294 274 147
BseYI C'CCAG_C
Cuts at: 1426 2184 2761 3257 1426
Size: 758 577 496 2170
Fragments arranged by size:
2170 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1543 1589 2797 104
Size: 1439 46 1208 1308
Fragments arranged by size:
1439 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 221 1015 1793 1983 2775 221
Size: 794 778 190 792 1447
Fragments arranged by size:
1447 794 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 221 1015 1626 1719 1793 1983 2045 2775 221
Size: 794 611 93 74 190 62 730 1447
Fragments arranged by size:
1447 794 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3221 3221
Size: 4001
BspHI T'CATG_A
Cuts at: 849 3177 849
Size: 2328 1673
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1051 1265 1567 1948 1051
Size: 214 302 381 3104
Fragments arranged by size:
3104 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2293 2347 3831 3996 50
Size: 2243 54 1484 165 55
Fragments arranged by size:
2243 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1913 1913
Size: 4001
BsrFI r'CCGG_y
Cuts at: 1999 2180 2389 1999
Size: 181 209 3611
Fragments arranged by size:
3611 209 181
BssSI C'ACGA_G
Cuts at: 2272 2630 2272
Size: 358 3643
BstAPI GCAn_nnn'nTGC
Cuts at: 1378 1378
Size: 4001
BstBI TT'CG_AA
Cuts at: 2362 2362
Size: 4001
BstYI r'GATC_y
Cuts at: 1 141 714 1026 1493 1516 1851 2097 3109
Size: 140 573 312 467 23 335 246 1012
Cuts at: 3109 3195 3207 3553 1
Size: 86 12 346 449
Fragments arranged by size:
1012 573 467 449 346 335 312 246
140 86 23 12
BtgI C'CryG_G
Cuts at: 265 265
Size: 4001
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1951 2132 2295 1951
Size: 181 163 3657
Fragments arranged by size:
3657 181 163
BtsI GCAGTG_nn'
Cuts at: 1244 1244
Size: 4001
DraI TTT'AAA
Cuts at: 591 1000 591
Size: 409 3592
DraIII CAC_nnn'GTG
Cuts at: 471 471
Size: 4001
DrdI GACnn_nn'nnGTC
Cuts at: 1707 2565 1707
Size: 858 3143
EaeI y'GGCC_r
Cuts at: 1586 1760 2151 2178 3523 1586
Size: 174 391 27 1345 2064
Fragments arranged by size:
2064 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1586 1586
Size: 4001
EarI CTCTTCn'nnn_
Cuts at: 254 656 2024 2234 254
Size: 402 1368 210 2021
Fragments arranged by size:
2021 1368 402 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2209 2519 2665 3811 2209
Size: 310 146 1146 2399
Fragments arranged by size:
2399 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1013 1013
Size: 4001
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 600 680 768 936 1826 2258 3005 3273 600
Size: 80 88 168 890 432 747 268 1328
Fragments arranged by size:
1328 890 747 432 268 168 88 80
EcoO109I rG'GnC_Cy
Cuts at: 156 1140 156
Size: 984 3017
EcoRI G'AATT_C
Cuts at: 650 1005 650
Size: 355 3646
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2020 2020
Size: 4001
FspI TGC'GCA
Cuts at: 1782 1782
Size: 4001
HaeII r_GCGC'y
Cuts at: 1435 1683 2705 3684 1435
Size: 248 1022 979 1752
Fragments arranged by size:
1752 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 228 260 699 731 1843 1873 1875 1905 228
Size: 32 439 32 1112 30 2 30 2324
Fragments arranged by size:
2324 1112 439 32 32 30 30 2
HincII GTy'rAC
Cuts at: 682 1040 682
Size: 358 3643
HindIII A'AGCT_T
Cuts at: 780 780
Size: 4001
KasI G'GCGC_C
Cuts at: 1431 1679 1431
Size: 248 3753
KpnI G_GTAC'C
Cuts at: 1021 1021
Size: 4001
MluI A'CGCG_T
Cuts at: 3434 3434
Size: 4001
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2672 2856 2672
Size: 184 3817
MscI TGG'CCA
Cuts at: 1762 1762
Size: 4001
MslI CAynn'nnrTG
Cuts at: 759 854 911 1990 2117 759
Size: 95 57 1079 127 2643
Fragments arranged by size:
2643 1079 127 95 57
MspA1I CmG'CkG
Cuts at: 1112 1426 1786 2799 3044 1112
Size: 314 360 1013 245 2069
Fragments arranged by size:
2069 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2182 2182
Size: 4001
NarI GG'CG_CC
Cuts at: 1432 1680 1432
Size: 248 3753
NcoI C'CATG_G
Cuts at: 265 265
Size: 4001
NdeI CA'TA_TG
Cuts at: 937 937
Size: 4001
NgoMIV G'CCGG_C
Cuts at: 2180 2180
Size: 4001
NspI r_CATG'y
Cuts at: 848 1054 1554 2461 848
Size: 206 500 907 2388
Fragments arranged by size:
2388 907 500 206
PciI A'CATG_T
Cuts at: 844 2457 844
Size: 1613 2388
PmeI GTTT'AAAC
Cuts at: 1000 1000
Size: 4001
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3140 3172 3140
Size: 32 3969
PstI C_TGCA'G
Cuts at: 1048 1733 1048
Size: 685 3316
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4001
PvuII CAG'CTG
Cuts at: 1426 1786 1426
Size: 360 3641
RsrII CG'GwC_CG
Cuts at: 2196 2196
Size: 4001
SacI G_AGCT'C
Cuts at: 1015 1015
Size: 4001
SalI G'TCGA_C
Cuts at: 1038 1038
Size: 4001
SapI GCTCTTCn'nnn_
Cuts at: 254 2024 2234 254
Size: 1770 210 2021
Fragments arranged by size:
2021 1770 210
SbfI CC_TGCA'GG
Cuts at: 1048 1048
Size: 4001
ScaI AGT'ACT
Cuts at: 3518 3518
Size: 4001
SexAI A'CCwGG_T
Cuts at: 992 992
Size: 4001
SfcI C'TryA_G
Cuts at: 33 314 1044 1729 2722 2913 33
Size: 281 730 685 993 191 1121
Fragments arranged by size:
1121 993 730 685 281 191
SfoI GGC'GCC
Cuts at: 1433 1681 1433
Size: 248 3753
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4001
SmaI CCC'GGG
Cuts at: 1023 1023
Size: 4001
SmlI C'TyrA_G
Cuts at: 1156 2071 2563 2825 1156
Size: 915 492 262 2332
Fragments arranged by size:
2332 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4001
SphI G_CATG'C
Cuts at: 1054 1554 1054
Size: 500 3501
StyI C'CwwG_G
Cuts at: 265 741 1135 1489 265
Size: 476 394 354 2777
Fragments arranged by size:
2777 476 394 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1584 1717 2176 2385 1584
Size: 133 459 209 3200
Fragments arranged by size:
3200 459 209 133
TatI w'GTAC_w
Cuts at: 3516 3516
Size: 4001
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3518 3550 3518
Size: 32 3969
Tth111I GACn'n_nGTC
Cuts at: 1798 1798
Size: 4001
XbaI T'CTAG_A
Cuts at: 246 1032 246
Size: 786 3215
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1496 1496
Size: 4001
XmaI C'CCGG_G
Cuts at: 1021 1021
Size: 4001
XmnI GAAnn'nnTTC
Cuts at: 613 781 613
Size: 168 3833
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HpaI MfeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI