Restriction map for
pF1KB9698
(Circular) (Six-base) MAPSORT of: pF1KB9698.seq Check: 9414 from: 1 to: 3986
pF1KB9698 3986 bp
With 182 enzymes: SgfI *
February 25, 2010 13:26 ..
AccI GT'mk_AC
Cuts at: 210 244 1024 210
Size: 34 780 3172
Fragments arranged by size:
3172 780 34
Acc65I G'GTAC_C
Cuts at: 1002 1002
Size: 3986
AclI AA'CG_TT
Cuts at: 3826 3826
Size: 3986
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 872 1811 2243 2990 3258 872
Size: 939 432 747 268 1600
Fragments arranged by size:
1600 939 747 432 268
AflIII A'CryG_T
Cuts at: 1338 2442 3419 1338
Size: 1104 977 1905
Fragments arranged by size:
1905 1104 977
AgeI A'CCGG_T
Cuts at: 2374 2374
Size: 3986
AleI CACnn'nnGTG
Cuts at: 235 235
Size: 3986
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 212 212
Size: 3986
AlwNI CAG_nnn'CTG
Cuts at: 421 1291 2858 421
Size: 870 1567 1549
Fragments arranged by size:
1567 1549 870
ApaLI G'TGCA_C
Cuts at: 2756 2756
Size: 3986
ApoI r'AATT_y
Cuts at: 153 346 520 598 990 3339 3595 153
Size: 193 174 78 392 2349 256 544
Fragments arranged by size:
2349 544 392 256 193 174 78
AseI AT'TA_AT
Cuts at: 20 20
Size: 3986
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3986
AvaI C'yCGr_G
Cuts at: 1006 1006
Size: 3986
BamHI G'GATC_C
Cuts at: 1011 1011
Size: 3986
BanI G'GyrC_C
Cuts at: 1002 1416 1664 1699 1002
Size: 414 248 35 3289
Fragments arranged by size:
3289 414 248 35
BanII G_rGCy'C
Cuts at: 1000 2030 1000
Size: 1030 2956
BbeI G_GCGC'C
Cuts at: 1420 1668 1420
Size: 248 3738
BciVI GTATCCnnnnn_n'
Cuts at: 541 1884 2645 3986 541
Size: 1343 761 1341 541
Fragments arranged by size:
1343 1341 761 541
BclI T'GATC_A
Cuts at: 1506 1506
Size: 3986
BfrBI ATG'CAT
Cuts at: 189 655 189
Size: 466 3520
BglII A'GATC_T
Cuts at: 1501 3538 1501
Size: 2037 1949
BlpI GC'TnA_GC
Cuts at: 640 698 1098 640
Size: 58 400 3528
Fragments arranged by size:
3528 400 58
Bme1580I G_kGCm'C
Cuts at: 1611 1704 2760 1611
Size: 93 1056 2837
Fragments arranged by size:
2837 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1198 1279 1614 3758 1198
Size: 81 335 2144 1426
Fragments arranged by size:
2144 1426 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2353 2353
Size: 3986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 470 709 814 886 470
Size: 239 105 72 3570
Fragments arranged by size:
3570 239 105 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 384 815 991 1162 2041 2533 2831 384
Size: 431 176 171 879 492 298 1539
Fragments arranged by size:
1539 879 492 431 298 176 171
BsaI GGTCTCn'nnnn_
Cuts at: 3690 3690
Size: 3986
BsaAI yAC'GTr
Cuts at: 1339 1339
Size: 3986
BsaBI GATnn'nnATC
Cuts at: 6 1500 3537 6
Size: 1494 2037 455
Fragments arranged by size:
2037 1494 455
BsaHI Gr'CG_yC
Cuts at: 161 1417 1665 2367 3863 161
Size: 1256 248 702 1496 284
Fragments arranged by size:
1496 1256 702 284 248
BsaWI w'CCGG_w
Cuts at: 899 1175 1696 2374 2648 2795 3206 899
Size: 276 521 678 274 147 411 1679
Fragments arranged by size:
1679 678 521 411 276 274 147
BseYI C'CCAG_C
Cuts at: 1411 2169 2746 3242 1411
Size: 758 577 496 2155
Fragments arranged by size:
2155 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 996 996
Size: 3986
BsiEI CG_ry'CG
Cuts at: 104 1528 1574 2782 104
Size: 1424 46 1208 1308
Fragments arranged by size:
1424 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1000 1778 1968 2760 1000
Size: 778 190 792 2226
Fragments arranged by size:
2226 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 525 525
Size: 3986
Bsp1286I G_dGCh'C
Cuts at: 1000 1611 1704 1778 1968 2030 2760 1000
Size: 611 93 74 190 62 730 2226
Fragments arranged by size:
2226 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3206 3206
Size: 3986
BspHI T'CATG_A
Cuts at: 839 3162 839
Size: 2323 1663
BspMI ACCTGCnnnn'nnnn_
Cuts at: 310 1036 1250 1552 1933 310
Size: 726 214 302 381 2363
Fragments arranged by size:
2363 726 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2278 2332 3816 3981 50
Size: 2228 54 1484 165 55
Fragments arranged by size:
2228 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 773 1898 773
Size: 1125 2861
BsrFI r'CCGG_y
Cuts at: 1984 2165 2374 1984
Size: 181 209 3596
Fragments arranged by size:
3596 209 181
BsrGI T'GTAC_A
Cuts at: 716 716
Size: 3986
BssSI C'ACGA_G
Cuts at: 2257 2615 2257
Size: 358 3628
BstAPI GCAn_nnn'nTGC
Cuts at: 1363 1363
Size: 3986
BstBI TT'CG_AA
Cuts at: 2347 2347
Size: 3986
BstXI CCAn_nnnn'nTGG
Cuts at: 687 687
Size: 3986
BstYI r'GATC_y
Cuts at: 1 1011 1478 1501 1836 2082 3094 3180 3192
Size: 1010 467 23 335 246 1012 86 12
Cuts at: 3192 3538 1
Size: 346 449
Fragments arranged by size:
1012 1010 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1936 2117 2280 1936
Size: 181 163 3642
Fragments arranged by size:
3642 181 163
BtsI GCAGTG_nn'
Cuts at: 1229 1229
Size: 3986
DraI TTT'AAA
Cuts at: 525 648 985 525
Size: 123 337 3526
Fragments arranged by size:
3526 337 123
DrdI GACnn_nn'nnGTC
Cuts at: 1692 2550 1692
Size: 858 3128
EaeI y'GGCC_r
Cuts at: 1571 1745 2136 2163 3508 1571
Size: 174 391 27 1345 2049
Fragments arranged by size:
2049 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1571 1571
Size: 3986
EarI CTCTTCn'nnn_
Cuts at: 401 458 2009 2219 401
Size: 57 1551 210 2168
Fragments arranged by size:
2168 1551 210 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 459 2194 2504 2650 3796 459
Size: 1735 310 146 1146 649
Fragments arranged by size:
1735 1146 649 310 146
EcoICRI GAG'CTC
Cuts at: 998 998
Size: 3986
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 470 709 814 872 886 1811 2243 2990 3258
Size: 239 105 58 14 925 432 747 268
Cuts at: 3258 470
Size: 1198
Fragments arranged by size:
1198 925 747 432 268 239 105 58
14
EcoO109I rG'GnC_Cy
Cuts at: 408 573 1125 408
Size: 165 552 3269
Fragments arranged by size:
3269 552 165
EcoRI G'AATT_C
Cuts at: 153 598 990 153
Size: 445 392 3149
Fragments arranged by size:
3149 445 392
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2005 2005
Size: 3986
FspI TGC'GCA
Cuts at: 1767 1767
Size: 3986
HaeII r_GCGC'y
Cuts at: 1420 1668 2690 3669 1420
Size: 248 1022 979 1737
Fragments arranged by size:
1737 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 174 206 1828 1858 1860 1890 174
Size: 32 1622 30 2 30 2270
Fragments arranged by size:
2270 1622 32 30 30 2
HincII GTy'rAC
Cuts at: 1025 1025
Size: 3986
HindIII A'AGCT_T
Cuts at: 119 119
Size: 3986
KasI G'GCGC_C
Cuts at: 1416 1664 1416
Size: 248 3738
KpnI G_GTAC'C
Cuts at: 1006 1006
Size: 3986
MluI A'CGCG_T
Cuts at: 3419 3419
Size: 3986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 245 2657 2841 245
Size: 2412 184 1390
Fragments arranged by size:
2412 1390 184
MscI TGG'CCA
Cuts at: 1747 1747
Size: 3986
MslI CAynn'nnrTG
Cuts at: 194 235 844 1975 2102 194
Size: 41 609 1131 127 2078
Fragments arranged by size:
2078 1131 609 127 41
MspA1I CmG'CkG
Cuts at: 418 583 1097 1411 1771 2784 3029 418
Size: 165 514 314 360 1013 245 1375
Fragments arranged by size:
1375 1013 514 360 314 245 165
NaeI GCC'GGC
Cuts at: 2167 2167
Size: 3986
NarI GG'CG_CC
Cuts at: 1417 1665 1417
Size: 248 3738
NgoMIV G'CCGG_C
Cuts at: 2165 2165
Size: 3986
NsiI A_TGCA'T
Cuts at: 191 657 191
Size: 466 3520
NspI r_CATG'y
Cuts at: 659 1039 1539 2446 659
Size: 380 500 907 2199
Fragments arranged by size:
2199 907 500 380
PciI A'CATG_T
Cuts at: 2442 2442
Size: 3986
PmeI GTTT'AAAC
Cuts at: 985 985
Size: 3986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3125 3157 3125
Size: 32 3954
PpuMI rG'GwC_Cy
Cuts at: 408 408
Size: 3986
PsiI TTA'TAA
Cuts at: 344 344
Size: 3986
PstI C_TGCA'G
Cuts at: 638 1033 1718 638
Size: 395 685 2906
Fragments arranged by size:
2906 685 395
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3986
PvuII CAG'CTG
Cuts at: 418 583 1411 1771 418
Size: 165 828 360 2633
Fragments arranged by size:
2633 828 360 165
RsrII CG'GwC_CG
Cuts at: 2181 2181
Size: 3986
SacI G_AGCT'C
Cuts at: 1000 1000
Size: 3986
SalI G'TCGA_C
Cuts at: 1023 1023
Size: 3986
SapI GCTCTTCn'nnn_
Cuts at: 458 2009 2219 458
Size: 1551 210 2225
Fragments arranged by size:
2225 1551 210
SbfI CC_TGCA'GG
Cuts at: 1033 1033
Size: 3986
ScaI AGT'ACT
Cuts at: 3503 3503
Size: 3986
SfcI C'TryA_G
Cuts at: 33 245 634 1029 1714 2707 2898 33
Size: 212 389 395 685 993 191 1121
Fragments arranged by size:
1121 993 685 395 389 212 191
SfoI GGC'GCC
Cuts at: 1418 1666 1418
Size: 248 3738
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3986
SmaI CCC'GGG
Cuts at: 1008 1008
Size: 3986
SmlI C'TyrA_G
Cuts at: 363 830 970 1141 2056 2548 2810 363
Size: 467 140 171 915 492 262 1539
Fragments arranged by size:
1539 915 492 467 262 171 140
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3986
SphI G_CATG'C
Cuts at: 659 1039 1539 659
Size: 380 500 3106
Fragments arranged by size:
3106 500 380
SspI AAT'ATT
Cuts at: 674 674
Size: 3986
StyI C'CwwG_G
Cuts at: 1120 1474 1120
Size: 354 3632
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 568 1569 1702 2161 2370 568
Size: 1001 133 459 209 2184
Fragments arranged by size:
2184 1001 459 209 133
TatI w'GTAC_w
Cuts at: 716 3501 716
Size: 2785 1201
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3503 3535 3503
Size: 32 3954
Tth111I GACn'n_nGTC
Cuts at: 1783 1783
Size: 3986
XbaI T'CTAG_A
Cuts at: 1017 1017
Size: 3986
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 562 1481 562
Size: 919 3067
XmaI C'CCGG_G
Cuts at: 1006 1006
Size: 3986
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI
NspI PciI PmeI PpiI PpuMI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BglI BmgBI BmtI
Bpu10I BsaXI BseRI BsiWI BsmI BssHII BstEII BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NcoI NdeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI ZraI