Restriction map for
pF1KB9690
(Circular) (Six-base) MAPSORT of: pF1KB9690.seq Check: 9937 from: 1 to: 3791
pF1KB9690 3791 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AatII G_ACGT'C
Cuts at: 185 185
Size: 3791
AccI GT'mk_AC
Cuts at: 829 829
Size: 3791
Acc65I G'GTAC_C
Cuts at: 807 807
Size: 3791
AclI AA'CG_TT
Cuts at: 3631 3631
Size: 3791
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 1616 2048 2795 3063 532
Size: 1084 432 747 268 1260
Fragments arranged by size:
1260 1084 747 432 268
AfeI AGC'GCT
Cuts at: 418 526 418
Size: 108 3683
AflIII A'CryG_T
Cuts at: 1143 2247 3224 1143
Size: 1104 977 1710
Fragments arranged by size:
1710 1104 977
AgeI A'CCGG_T
Cuts at: 403 2179 403
Size: 1776 2015
AlwNI CAG_nnn'CTG
Cuts at: 1096 2663 1096
Size: 1567 2224
ApaI G_GGCC'C
Cuts at: 218 218
Size: 3791
ApaLI G'TGCA_C
Cuts at: 2561 2561
Size: 3791
ApoI r'AATT_y
Cuts at: 795 3144 3400 795
Size: 2349 256 1186
Fragments arranged by size:
2349 1186 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3791
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
AvaI C'yCGr_G
Cuts at: 211 322 410 811 211
Size: 111 88 401 3191
Fragments arranged by size:
3191 401 111 88
BamHI G'GATC_C
Cuts at: 816 816
Size: 3791
BanI G'GyrC_C
Cuts at: 192 400 807 1221 1469 1504 192
Size: 208 407 414 248 35 2479
Fragments arranged by size:
2479 414 407 248 208 35
BanII G_rGCy'C
Cuts at: 218 705 805 1835 218
Size: 487 100 1030 2174
Fragments arranged by size:
2174 1030 487 100
BbeI G_GCGC'C
Cuts at: 196 1225 1473 196
Size: 1029 248 2514
Fragments arranged by size:
2514 1029 248
BbsI GAAGACnn'nnnn_
Cuts at: 284 284
Size: 3791
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 401 435 401
Size: 34 3757
BciVI GTATCCnnnnn_n'
Cuts at: 1689 2450 3791 1689
Size: 761 1341 1689
Fragments arranged by size:
1689 1341 761
BclI T'GATC_A
Cuts at: 1311 1311
Size: 3791
BglI GCCn_nnn'nGGC
Cuts at: 213 515 604 213
Size: 302 89 3400
Fragments arranged by size:
3400 302 89
BglII A'GATC_T
Cuts at: 1306 3343 1306
Size: 2037 1754
BlpI GC'TnA_GC
Cuts at: 457 903 457
Size: 446 3345
Bme1580I G_kGCm'C
Cuts at: 218 1416 1509 2565 218
Size: 1198 93 1056 1444
Fragments arranged by size:
1444 1198 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 153 701 1003 1084 1419 3563 153
Size: 548 302 81 335 2144 381
Fragments arranged by size:
2144 548 381 335 302 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2158 2158
Size: 3791
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 187
Size: 3791
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 967 1846 2338 2636 967
Size: 879 492 298 2122
Fragments arranged by size:
2122 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3495 3495
Size: 3791
BsaAI yAC'GTr
Cuts at: 1144 1144
Size: 3791
BsaBI GATnn'nnATC
Cuts at: 6 1305 3342 6
Size: 1299 2037 455
Fragments arranged by size:
2037 1299 455
BsaHI Gr'CG_yC
Cuts at: 182 193 1222 1470 2172 3668 182
Size: 11 1029 248 702 1496 305
Fragments arranged by size:
1496 1029 702 305 248 11
BsaWI w'CCGG_w
Cuts at: 403 611 980 1501 2179 2453 2600 3011 403
Size: 208 369 521 678 274 147 411 1183
Fragments arranged by size:
1183 678 521 411 369 274 208 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 319 319
Size: 3791
BseYI C'CCAG_C
Cuts at: 217 1216 1974 2551 3047 217
Size: 999 758 577 496 961
Fragments arranged by size:
999 961 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 405 405
Size: 3791
BsiEI CG_ry'CG
Cuts at: 104 137 1333 1379 2587 104
Size: 33 1196 46 1208 1308
Fragments arranged by size:
1308 1208 1196 46 33
BsiHKAI G_wGCw'C
Cuts at: 805 1583 1773 2565 805
Size: 778 190 792 2031
Fragments arranged by size:
2031 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 218 705 805 1416 1509 1583 1773 1835 2565
Size: 487 100 611 93 74 190 62 730
Cuts at: 2565 218
Size: 1444
Fragments arranged by size:
1444 730 611 487 190 100 93 74
62
BspEI T'CCGG_A
Cuts at: 611 3011 611
Size: 2400 1391
BspHI T'CATG_A
Cuts at: 2967 2967
Size: 3791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 841 1055 1357 1738 841
Size: 214 302 381 2894
Fragments arranged by size:
2894 381 302 214
BsrBI CCG'CTC
Cuts at: 50 375 579 2083 2137 3621 3786 50
Size: 325 204 1504 54 1484 165 55
Fragments arranged by size:
1504 1484 325 204 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1703 1703
Size: 3791
BsrFI r'CCGG_y
Cuts at: 228 397 403 1789 1970 2179 228
Size: 169 6 1386 181 209 1840
Fragments arranged by size:
1840 1386 209 181 169 6
BsrGI T'GTAC_A
Cuts at: 767 767
Size: 3791
BssHII G'CGCG_C
Cuts at: 476 478 476
Size: 2 3789
BssSI C'ACGA_G
Cuts at: 2062 2420 2062
Size: 358 3433
BstAPI GCAn_nnn'nTGC
Cuts at: 1168 1168
Size: 3791
BstBI TT'CG_AA
Cuts at: 2152 2152
Size: 3791
BstXI CCAn_nnnn'nTGG
Cuts at: 714 714
Size: 3791
BstYI r'GATC_y
Cuts at: 1 816 1283 1306 1641 1887 2899 2985 2997
Size: 815 467 23 335 246 1012 86 12
Cuts at: 2997 3343 1
Size: 346 449
Fragments arranged by size:
1012 815 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 383 383
Size: 3791
BtgI C'CryG_G
Cuts at: 107 131 196 428 492 107
Size: 24 65 232 64 3406
Fragments arranged by size:
3406 232 65 64 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1741 1922 2085 1741
Size: 181 163 3447
Fragments arranged by size:
3447 181 163
BtsI GCAGTG_nn'
Cuts at: 1034 1034
Size: 3791
ClaI AT'CG_AT
Cuts at: 152 152
Size: 3791
DraI TTT'AAA
Cuts at: 790 790
Size: 3791
DraIII CAC_nnn'GTG
Cuts at: 498 498
Size: 3791
DrdI GACnn_nn'nnGTC
Cuts at: 1497 2355 1497
Size: 858 2933
EaeI y'GGCC_r
Cuts at: 110 134 333 593 716 1376 1550 1941 1968
Size: 24 199 260 123 660 174 391 27
Cuts at: 1968 3313 110
Size: 1345 588
Fragments arranged by size:
1345 660 588 391 260 199 174 123
27 24
EagI C'GGCC_G
Cuts at: 134 1376 134
Size: 1242 2549
EarI CTCTTCn'nnn_
Cuts at: 170 284 1814 2024 170
Size: 114 1530 210 1937
Fragments arranged by size:
1937 1530 210 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 247 1999 2309 2455 3601 247
Size: 1752 310 146 1146 437
Fragments arranged by size:
1752 1146 437 310 146
EcoICRI GAG'CTC
Cuts at: 803 803
Size: 3791
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 532 1616 2048 2795 3063 187
Size: 345 1084 432 747 268 915
Fragments arranged by size:
1084 915 747 432 345 268
EcoO109I rG'GnC_Cy
Cuts at: 930 930
Size: 3791
EcoRI G'AATT_C
Cuts at: 795 795
Size: 3791
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 757 1810 757
Size: 1053 2738
FspI TGC'GCA
Cuts at: 1572 1572
Size: 3791
HaeII r_GCGC'y
Cuts at: 196 420 528 610 1225 1473 2495 3474 196
Size: 224 108 82 615 248 1022 979 513
Fragments arranged by size:
1022 979 615 513 248 224 108 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1633 1663 1665 1695 1633
Size: 30 2 30 3729
Fragments arranged by size:
3729 30 30 2
HincII GTy'rAC
Cuts at: 830 830
Size: 3791
KasI G'GCGC_C
Cuts at: 192 1221 1469 192
Size: 1029 248 2514
Fragments arranged by size:
2514 1029 248
KpnI G_GTAC'C
Cuts at: 811 811
Size: 3791
MluI A'CGCG_T
Cuts at: 3224 3224
Size: 3791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2462 2646 2462
Size: 184 3607
MscI TGG'CCA
Cuts at: 718 1552 718
Size: 834 2957
MslI CAynn'nnrTG
Cuts at: 1780 1907 1780
Size: 127 3664
MspA1I CmG'CkG
Cuts at: 133 198 348 430 902 1216 1576 2589 2834
Size: 65 150 82 472 314 360 1013 245
Cuts at: 2834 133
Size: 1090
Fragments arranged by size:
1090 1013 472 360 314 245 150 82
65
NaeI GCC'GGC
Cuts at: 230 399 1972 230
Size: 169 1573 2049
Fragments arranged by size:
2049 1573 169
NarI GG'CG_CC
Cuts at: 193 1222 1470 193
Size: 1029 248 2514
Fragments arranged by size:
2514 1029 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3791
NgoMIV G'CCGG_C
Cuts at: 228 397 1970 228
Size: 169 1573 2049
Fragments arranged by size:
2049 1573 169
NspI r_CATG'y
Cuts at: 844 1344 2251 844
Size: 500 907 2384
Fragments arranged by size:
2384 907 500
PciI A'CATG_T
Cuts at: 2247 2247
Size: 3791
PflMI CCAn_nnn'nTGG
Cuts at: 498 498
Size: 3791
PfoI T'CCnGG_A
Cuts at: 649 649
Size: 3791
PmeI GTTT'AAAC
Cuts at: 790 790
Size: 3791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2930 2962 2930
Size: 32 3759
PspOMI G'GGCC_C
Cuts at: 214 214
Size: 3791
PstI C_TGCA'G
Cuts at: 838 1523 838
Size: 685 3106
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3791
PvuII CAG'CTG
Cuts at: 1216 1576 1216
Size: 360 3431
RsrII CG'GwC_CG
Cuts at: 1986 1986
Size: 3791
SacI G_AGCT'C
Cuts at: 805 805
Size: 3791
SacII CC_GC'GG
Cuts at: 134 199 431 134
Size: 65 232 3494
Fragments arranged by size:
3494 232 65
SalI G'TCGA_C
Cuts at: 828 828
Size: 3791
SapI GCTCTTCn'nnn_
Cuts at: 1814 2024 1814
Size: 210 3581
SbfI CC_TGCA'GG
Cuts at: 838 838
Size: 3791
ScaI AGT'ACT
Cuts at: 3308 3308
Size: 3791
SfcI C'TryA_G
Cuts at: 33 753 834 1519 2512 2703 33
Size: 720 81 685 993 191 1121
Fragments arranged by size:
1121 993 720 685 191 81
SfoI GGC'GCC
Cuts at: 194 1223 1471 194
Size: 1029 248 2514
Fragments arranged by size:
2514 1029 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
SgrAI Cr'CCGG_yG
Cuts at: 228 228
Size: 3791
SmaI CCC'GGG
Cuts at: 213 324 813 213
Size: 111 489 3191
Fragments arranged by size:
3191 489 111
SmlI C'TyrA_G
Cuts at: 946 1861 2353 2615 946
Size: 915 492 262 2122
Fragments arranged by size:
2122 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3791
SphI G_CATG'C
Cuts at: 844 1344 844
Size: 500 3291
StuI AGG'CCT
Cuts at: 388 388
Size: 3791
StyI C'CwwG_G
Cuts at: 107 741 925 1279 107
Size: 634 184 354 2619
Fragments arranged by size:
2619 634 354 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1374 1507 1966 2175 1374
Size: 133 459 209 2990
Fragments arranged by size:
2990 459 209 133
TatI w'GTAC_w
Cuts at: 767 3306 767
Size: 2539 1252
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3308 3340 3308
Size: 32 3759
Tth111I GACn'n_nGTC
Cuts at: 1588 1588
Size: 3791
XbaI T'CTAG_A
Cuts at: 822 822
Size: 3791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 564 1286 564
Size: 722 3069
XmaI C'CCGG_G
Cuts at: 211 322 811 211
Size: 111 489 3191
Fragments arranged by size:
3191 489 111
XmnI GAAnn'nnTTC
Cuts at: 664 664
Size: 3791
ZraI GAC'GTC
Cuts at: 183 183
Size: 3791
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI BsiWI
BsmI BsmBI BstEII BstZ17I CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PpuMI PshAI PsiI PspXI
PsrI SanDI SexAI SfiI SnaBI SrfI SspI SwaI
XhoI