Restriction map for
pF1KB9688
(Circular) (Six-base) MAPSORT of: pF1KB9688.seq Check: 7903 from: 1 to: 3779
pF1KB9688 3779 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 290 817 290
Size: 527 3252
Acc65I G'GTAC_C
Cuts at: 795 795
Size: 3779
AclI AA'CG_TT
Cuts at: 3619 3619
Size: 3779
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1604 2036 2783 3051 1604
Size: 432 747 268 2332
Fragments arranged by size:
2332 747 432 268
AflII C'TTAA_G
Cuts at: 240 240
Size: 3779
AflIII A'CryG_T
Cuts at: 1131 2235 3212 1131
Size: 1104 977 1698
Fragments arranged by size:
1698 1104 977
AgeI A'CCGG_T
Cuts at: 2167 2167
Size: 3779
AlwNI CAG_nnn'CTG
Cuts at: 221 609 1084 2651 221
Size: 388 475 1567 1349
Fragments arranged by size:
1567 1349 475 388
ApaLI G'TGCA_C
Cuts at: 2549 2549
Size: 3779
ApoI r'AATT_y
Cuts at: 625 783 3132 3388 625
Size: 158 2349 256 1016
Fragments arranged by size:
2349 1016 256 158
AseI AT'TA_AT
Cuts at: 20 20
Size: 3779
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3779
AvaI C'yCGr_G
Cuts at: 308 394 616 799 308
Size: 86 222 183 3288
Fragments arranged by size:
3288 222 183 86
BamHI G'GATC_C
Cuts at: 804 804
Size: 3779
BanI G'GyrC_C
Cuts at: 795 1209 1457 1492 795
Size: 414 248 35 3082
Fragments arranged by size:
3082 414 248 35
BanII G_rGCy'C
Cuts at: 115 148 315 793 1823 115
Size: 33 167 478 1030 2071
Fragments arranged by size:
2071 1030 478 167 33
BbeI G_GCGC'C
Cuts at: 1213 1461 1213
Size: 248 3531
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 651 685 651
Size: 34 3745
BciVI GTATCCnnnnn_n'
Cuts at: 1677 2438 3779 1677
Size: 761 1341 1677
Fragments arranged by size:
1677 1341 761
BclI T'GATC_A
Cuts at: 1299 1299
Size: 3779
BglII A'GATC_T
Cuts at: 707 1294 3331 707
Size: 587 2037 1155
Fragments arranged by size:
2037 1155 587
BlpI GC'TnA_GC
Cuts at: 891 891
Size: 3779
Bme1580I G_kGCm'C
Cuts at: 1404 1497 2553 1404
Size: 93 1056 2630
Fragments arranged by size:
2630 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 991 1072 1407 3551 991
Size: 81 335 2144 1219
Fragments arranged by size:
2144 1219 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2146 2146
Size: 3779
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 451
Size: 3779
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 955 1834 2326 2624 955
Size: 879 492 298 2110
Fragments arranged by size:
2110 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3483 3483
Size: 3779
BsaAI yAC'GTr
Cuts at: 120 1132 120
Size: 1012 2767
BsaBI GATnn'nnATC
Cuts at: 6 706 1293 3330 6
Size: 700 587 2037 455
Fragments arranged by size:
2037 700 587 455
BsaHI Gr'CG_yC
Cuts at: 1210 1458 2160 3656 1210
Size: 248 702 1496 1333
Fragments arranged by size:
1496 1333 702 248
BsaWI w'CCGG_w
Cuts at: 968 1489 2167 2441 2588 2999 968
Size: 521 678 274 147 411 1748
Fragments arranged by size:
1748 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 374 448 481 374
Size: 74 33 3672
Fragments arranged by size:
3672 74 33
BseYI C'CCAG_C
Cuts at: 1204 1962 2539 3035 1204
Size: 758 577 496 1948
Fragments arranged by size:
1948 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 532 226
Size: 306 3473
BsiEI CG_ry'CG
Cuts at: 104 324 347 1321 1367 2575 104
Size: 220 23 974 46 1208 1308
Fragments arranged by size:
1308 1208 974 220 46 23
BsiHKAI G_wGCw'C
Cuts at: 115 793 1571 1761 2553 115
Size: 678 778 190 792 1341
Fragments arranged by size:
1341 792 778 678 190
BsmBI CGTCTCn'nnnn_
Cuts at: 365 556 365
Size: 191 3588
Bsp1286I G_dGCh'C
Cuts at: 115 148 315 793 1404 1497 1571 1761 1823
Size: 33 167 478 611 93 74 190 62
Cuts at: 1823 2553 115
Size: 730 1341
Fragments arranged by size:
1341 730 611 478 190 167 93 74
62 33
BspEI T'CCGG_A
Cuts at: 2999 2999
Size: 3779
BspHI T'CATG_A
Cuts at: 2955 2955
Size: 3779
BspMI ACCTGCnnnn'nnnn_
Cuts at: 829 1043 1345 1726 829
Size: 214 302 381 2882
Fragments arranged by size:
2882 381 302 214
BsrBI CCG'CTC
Cuts at: 50 349 2071 2125 3609 3774 50
Size: 299 1722 54 1484 165 55
Fragments arranged by size:
1722 1484 299 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1691 1691
Size: 3779
BsrFI r'CCGG_y
Cuts at: 510 1777 1958 2167 510
Size: 1267 181 209 2122
Fragments arranged by size:
2122 1267 209 181
BsrGI T'GTAC_A
Cuts at: 401 401
Size: 3779
BssSI C'ACGA_G
Cuts at: 559 2050 2408 559
Size: 1491 358 1930
Fragments arranged by size:
1930 1491 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1156 1156
Size: 3779
BstBI TT'CG_AA
Cuts at: 2140 2140
Size: 3779
BstYI r'GATC_y
Cuts at: 1 707 804 1271 1294 1629 1875 2887 2973
Size: 706 97 467 23 335 246 1012 86
Cuts at: 2973 2985 3331 1
Size: 12 346 449
Fragments arranged by size:
1012 706 467 449 346 335 246 97
86 23 12
BtgI C'CryG_G
Cuts at: 282 341 532 282
Size: 59 191 3529
Fragments arranged by size:
3529 191 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1729 1910 2073 1729
Size: 181 163 3435
Fragments arranged by size:
3435 181 163
BtsI GCAGTG_nn'
Cuts at: 456 1022 456
Size: 566 3213
DraI TTT'AAA
Cuts at: 778 778
Size: 3779
DrdI GACnn_nn'nnGTC
Cuts at: 1485 2343 1485
Size: 858 2921
EaeI y'GGCC_r
Cuts at: 344 1364 1538 1929 1956 3301 344
Size: 1020 174 391 27 1345 822
Fragments arranged by size:
1345 1020 822 391 174 27
EagI C'GGCC_G
Cuts at: 344 1364 344
Size: 1020 2759
EarI CTCTTCn'nnn_
Cuts at: 1802 2012 1802
Size: 210 3569
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 245 1987 2297 2443 3589 245
Size: 1742 310 146 1146 435
Fragments arranged by size:
1742 1146 435 310 146
EcoICRI GAG'CTC
Cuts at: 113 791 113
Size: 678 3101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 1604 2036 2783 3051 451
Size: 1153 432 747 268 1179
Fragments arranged by size:
1179 1153 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 918 918
Size: 3779
EcoRI G'AATT_C
Cuts at: 625 783 625
Size: 158 3621
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 266 787 1798 266
Size: 521 1011 2247
Fragments arranged by size:
2247 1011 521
FspI TGC'GCA
Cuts at: 1560 1560
Size: 3779
HaeII r_GCGC'y
Cuts at: 341 430 1213 1461 2483 3462 341
Size: 89 783 248 1022 979 658
Fragments arranged by size:
1022 979 783 658 248 89
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 390 422 1621 1651 1653 1683 390
Size: 32 1199 30 2 30 2486
Fragments arranged by size:
2486 1199 32 30 30 2
HincII GTy'rAC
Cuts at: 818 818
Size: 3779
KasI G'GCGC_C
Cuts at: 1209 1457 1209
Size: 248 3531
KpnI G_GTAC'C
Cuts at: 799 799
Size: 3779
MluI A'CGCG_T
Cuts at: 3212 3212
Size: 3779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 296 2450 2634 296
Size: 2154 184 1441
Fragments arranged by size:
2154 1441 184
MscI TGG'CCA
Cuts at: 1540 1540
Size: 3779
MslI CAynn'nnrTG
Cuts at: 1768 1895 1768
Size: 127 3652
MspA1I CmG'CkG
Cuts at: 343 890 1204 1564 2577 2822 343
Size: 547 314 360 1013 245 1300
Fragments arranged by size:
1300 1013 547 360 314 245
NaeI GCC'GGC
Cuts at: 1960 1960
Size: 3779
NarI GG'CG_CC
Cuts at: 1210 1458 1210
Size: 248 3531
NgoMIV G'CCGG_C
Cuts at: 1958 1958
Size: 3779
NotI GC'GGCC_GC
Cuts at: 344 344
Size: 3779
NspI r_CATG'y
Cuts at: 171 832 1332 2239 171
Size: 661 500 907 1711
Fragments arranged by size:
1711 907 661 500
PciI A'CATG_T
Cuts at: 2235 2235
Size: 3779
PflMI CCAn_nnn'nTGG
Cuts at: 609 609
Size: 3779
PfoI T'CCnGG_A
Cuts at: 351 351
Size: 3779
PmeI GTTT'AAAC
Cuts at: 778 778
Size: 3779
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2918 2950 2918
Size: 32 3747
PstI C_TGCA'G
Cuts at: 337 826 1511 337
Size: 489 685 2605
Fragments arranged by size:
2605 685 489
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3779
PvuII CAG'CTG
Cuts at: 1204 1564 1204
Size: 360 3419
RsrII CG'GwC_CG
Cuts at: 1974 1974
Size: 3779
SacI G_AGCT'C
Cuts at: 115 793 115
Size: 678 3101
SacII CC_GC'GG
Cuts at: 344 344
Size: 3779
SalI G'TCGA_C
Cuts at: 816 816
Size: 3779
SapI GCTCTTCn'nnn_
Cuts at: 1802 2012 1802
Size: 210 3569
SbfI CC_TGCA'GG
Cuts at: 826 826
Size: 3779
ScaI AGT'ACT
Cuts at: 3296 3296
Size: 3779
SfcI C'TryA_G
Cuts at: 33 333 822 1507 2500 2691 33
Size: 300 489 685 993 191 1121
Fragments arranged by size:
1121 993 685 489 300 191
SfoI GGC'GCC
Cuts at: 1211 1459 1211
Size: 248 3531
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3779
SmaI CCC'GGG
Cuts at: 310 396 801 310
Size: 86 405 3288
Fragments arranged by size:
3288 405 86
SmlI C'TyrA_G
Cuts at: 240 616 934 1849 2341 2603 240
Size: 376 318 915 492 262 1416
Fragments arranged by size:
1416 915 492 376 318 262
SnaBI TAC'GTA
Cuts at: 120 120
Size: 3779
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3779
SphI G_CATG'C
Cuts at: 832 1332 832
Size: 500 3279
StyI C'CwwG_G
Cuts at: 913 1267 913
Size: 354 3425
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1362 1495 1954 2163 1362
Size: 133 459 209 2978
Fragments arranged by size:
2978 459 209 133
TatI w'GTAC_w
Cuts at: 401 3294 401
Size: 2893 886
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3296 3328 3296
Size: 32 3747
Tth111I GACn'n_nGTC
Cuts at: 1576 1576
Size: 3779
XbaI T'CTAG_A
Cuts at: 810 810
Size: 3779
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1274 1274
Size: 3779
XhoI C'TCGA_G
Cuts at: 616 616
Size: 3779
XmaI C'CCGG_G
Cuts at: 308 394 799 308
Size: 86 405 3288
Fragments arranged by size:
3288 405 86
XmnI GAAnn'nnTTC
Cuts at: 625 625
Size: 3779
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NotI NspI
PciI PflMI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NruI
NsiI PacI PasI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SanDI SexAI SfiI SgrAI SrfI SspI
StuI SwaI ZraI