Restriction map for
pF1KB9677
(Circular) (Six-base) MAPSORT of: pF1KB9677.seq Check: 3440 from: 1 to: 3425
pF1KB9677 3425 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 463 463
Size: 3425
Acc65I G'GTAC_C
Cuts at: 441 441
Size: 3425
AclI AA'CG_TT
Cuts at: 3265 3265
Size: 3425
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1250 1682 2429 2697 1250
Size: 432 747 268 1978
Fragments arranged by size:
1978 747 432 268
AflIII A'CryG_T
Cuts at: 777 1881 2858 777
Size: 1104 977 1344
Fragments arranged by size:
1344 1104 977
AgeI A'CCGG_T
Cuts at: 1813 1813
Size: 3425
AlwNI CAG_nnn'CTG
Cuts at: 730 2297 730
Size: 1567 1858
ApaLI G'TGCA_C
Cuts at: 2195 2195
Size: 3425
ApoI r'AATT_y
Cuts at: 429 2778 3034 429
Size: 2349 256 820
Fragments arranged by size:
2349 820 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3425
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3425
AvaI C'yCGr_G
Cuts at: 445 445
Size: 3425
BamHI G'GATC_C
Cuts at: 450 450
Size: 3425
BanI G'GyrC_C
Cuts at: 441 855 1103 1138 441
Size: 414 248 35 2728
Fragments arranged by size:
2728 414 248 35
BanII G_rGCy'C
Cuts at: 439 1469 439
Size: 1030 2395
BbeI G_GCGC'C
Cuts at: 859 1107 859
Size: 248 3177
BciVI GTATCCnnnnn_n'
Cuts at: 1323 2084 3425 1323
Size: 761 1341 1323
Fragments arranged by size:
1341 1323 761
BclI T'GATC_A
Cuts at: 945 945
Size: 3425
BglII A'GATC_T
Cuts at: 940 2977 940
Size: 2037 1388
BlpI GC'TnA_GC
Cuts at: 537 537
Size: 3425
Bme1580I G_kGCm'C
Cuts at: 1050 1143 2199 1050
Size: 93 1056 2276
Fragments arranged by size:
2276 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 637 718 1053 3197 637
Size: 81 335 2144 865
Fragments arranged by size:
2144 865 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1792 1792
Size: 3425
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 250
Size: 3425
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 1480 1972 2270 601
Size: 879 492 298 1756
Fragments arranged by size:
1756 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3129 3129
Size: 3425
BsaAI yAC'GTr
Cuts at: 778 778
Size: 3425
BsaBI GATnn'nnATC
Cuts at: 6 939 2976 6
Size: 933 2037 455
Fragments arranged by size:
2037 933 455
BsaHI Gr'CG_yC
Cuts at: 856 1104 1806 3302 856
Size: 248 702 1496 979
Fragments arranged by size:
1496 979 702 248
BsaWI w'CCGG_w
Cuts at: 614 1135 1813 2087 2234 2645 614
Size: 521 678 274 147 411 1394
Fragments arranged by size:
1394 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 214 217 229 232 214
Size: 3 12 3 3407
Fragments arranged by size:
3407 12 3 3
BseYI C'CCAG_C
Cuts at: 850 1608 2185 2681 850
Size: 758 577 496 1594
Fragments arranged by size:
1594 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 967 1013 2221 104
Size: 863 46 1208 1308
Fragments arranged by size:
1308 1208 863 46
BsiHKAI G_wGCw'C
Cuts at: 439 1217 1407 2199 439
Size: 778 190 792 1665
Fragments arranged by size:
1665 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 439 1050 1143 1217 1407 1469 2199 439
Size: 611 93 74 190 62 730 1665
Fragments arranged by size:
1665 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2645 2645
Size: 3425
BspHI T'CATG_A
Cuts at: 2601 2601
Size: 3425
BspMI ACCTGCnnnn'nnnn_
Cuts at: 475 689 991 1372 475
Size: 214 302 381 2528
Fragments arranged by size:
2528 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1717 1771 3255 3420 50
Size: 1667 54 1484 165 55
Fragments arranged by size:
1667 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 312 1337 312
Size: 1025 2400
BsrFI r'CCGG_y
Cuts at: 1423 1604 1813 1423
Size: 181 209 3035
Fragments arranged by size:
3035 209 181
BssSI C'ACGA_G
Cuts at: 1696 2054 1696
Size: 358 3067
BstAPI GCAn_nnn'nTGC
Cuts at: 802 802
Size: 3425
BstBI TT'CG_AA
Cuts at: 1786 1786
Size: 3425
BstYI r'GATC_y
Cuts at: 1 450 917 940 1275 1521 2533 2619 2631
Size: 449 467 23 335 246 1012 86 12
Cuts at: 2631 2977 1
Size: 346 449
Fragments arranged by size:
1012 467 449 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 182 182
Size: 3425
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1375 1556 1719 1375
Size: 181 163 3081
Fragments arranged by size:
3081 181 163
BtsI GCAGTG_nn'
Cuts at: 668 668
Size: 3425
DraI TTT'AAA
Cuts at: 276 424 276
Size: 148 3277
DrdI GACnn_nn'nnGTC
Cuts at: 1131 1989 1131
Size: 858 2567
EaeI y'GGCC_r
Cuts at: 1010 1184 1575 1602 2947 1010
Size: 174 391 27 1345 1488
Fragments arranged by size:
1488 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1010 1010
Size: 3425
EarI CTCTTCn'nnn_
Cuts at: 164 191 1448 1658 164
Size: 27 1257 210 1931
Fragments arranged by size:
1931 1257 210 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1633 1943 2089 3235 1633
Size: 310 146 1146 1823
Fragments arranged by size:
1823 1146 310 146
EcoICRI GAG'CTC
Cuts at: 437 437
Size: 3425
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 1250 1682 2429 2697 250
Size: 1000 432 747 268 978
Fragments arranged by size:
1000 978 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 564 564
Size: 3425
EcoRI G'AATT_C
Cuts at: 429 429
Size: 3425
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1444 1444
Size: 3425
FspI TGC'GCA
Cuts at: 1206 1206
Size: 3425
HaeII r_GCGC'y
Cuts at: 859 1107 2129 3108 859
Size: 248 1022 979 1176
Fragments arranged by size:
1176 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 246 278 1267 1297 1299 1329 246
Size: 32 989 30 2 30 2342
Fragments arranged by size:
2342 989 32 30 30 2
HincII GTy'rAC
Cuts at: 464 464
Size: 3425
KasI G'GCGC_C
Cuts at: 855 1103 855
Size: 248 3177
KpnI G_GTAC'C
Cuts at: 445 445
Size: 3425
MluI A'CGCG_T
Cuts at: 2858 2858
Size: 3425
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 361 2096 2280 361
Size: 1735 184 1506
Fragments arranged by size:
1735 1506 184
MscI TGG'CCA
Cuts at: 1186 1186
Size: 3425
MslI CAynn'nnrTG
Cuts at: 1414 1541 1414
Size: 127 3298
MspA1I CmG'CkG
Cuts at: 536 850 1210 2223 2468 536
Size: 314 360 1013 245 1493
Fragments arranged by size:
1493 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1606 1606
Size: 3425
NarI GG'CG_CC
Cuts at: 856 1104 856
Size: 248 3177
NgoMIV G'CCGG_C
Cuts at: 1604 1604
Size: 3425
NspI r_CATG'y
Cuts at: 478 978 1885 478
Size: 500 907 2018
Fragments arranged by size:
2018 907 500
PciI A'CATG_T
Cuts at: 1881 1881
Size: 3425
PfoI T'CCnGG_A
Cuts at: 266 266
Size: 3425
PmeI GTTT'AAAC
Cuts at: 424 424
Size: 3425
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2564 2596 2564
Size: 32 3393
PstI C_TGCA'G
Cuts at: 472 1157 472
Size: 685 2740
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3425
PvuII CAG'CTG
Cuts at: 850 1210 850
Size: 360 3065
RsrII CG'GwC_CG
Cuts at: 1620 1620
Size: 3425
SacI G_AGCT'C
Cuts at: 439 439
Size: 3425
SalI G'TCGA_C
Cuts at: 462 462
Size: 3425
SapI GCTCTTCn'nnn_
Cuts at: 164 1448 1658 164
Size: 1284 210 1931
Fragments arranged by size:
1931 1284 210
SbfI CC_TGCA'GG
Cuts at: 472 472
Size: 3425
ScaI AGT'ACT
Cuts at: 2942 2942
Size: 3425
SfcI C'TryA_G
Cuts at: 33 468 1153 2146 2337 33
Size: 435 685 993 191 1121
Fragments arranged by size:
1121 993 685 435 191
SfoI GGC'GCC
Cuts at: 857 1105 857
Size: 248 3177
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3425
SmaI CCC'GGG
Cuts at: 447 447
Size: 3425
SmlI C'TyrA_G
Cuts at: 580 1495 1987 2249 580
Size: 915 492 262 1756
Fragments arranged by size:
1756 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3425
SphI G_CATG'C
Cuts at: 478 978 478
Size: 500 2925
StyI C'CwwG_G
Cuts at: 559 913 559
Size: 354 3071
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1008 1141 1600 1809 1008
Size: 133 459 209 2624
Fragments arranged by size:
2624 459 209 133
TatI w'GTAC_w
Cuts at: 2940 2940
Size: 3425
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2942 2974 2942
Size: 32 3393
Tth111I GACn'n_nGTC
Cuts at: 1222 1222
Size: 3425
XbaI T'CTAG_A
Cuts at: 456 456
Size: 3425
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 920 920
Size: 3425
XmaI C'CCGG_G
Cuts at: 445 445
Size: 3425
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI