Restriction map for
pF1KB9675
(Circular) (Six-base) MAPSORT of: pF1KB9675.seq Check: 2320 from: 1 to: 3365
pF1KB9675 3365 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 403 403
Size: 3365
Acc65I G'GTAC_C
Cuts at: 381 381
Size: 3365
AclI AA'CG_TT
Cuts at: 3205 3205
Size: 3365
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 245 1190 1622 2369 2637 214
Size: 31 945 432 747 268 942
Fragments arranged by size:
945 942 747 432 268 31
AflIII A'CryG_T
Cuts at: 717 1821 2798 717
Size: 1104 977 1284
Fragments arranged by size:
1284 1104 977
AgeI A'CCGG_T
Cuts at: 1753 1753
Size: 3365
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 165 165
Size: 3365
AlwNI CAG_nnn'CTG
Cuts at: 670 2237 670
Size: 1567 1798
ApaLI G'TGCA_C
Cuts at: 2135 2135
Size: 3365
ApoI r'AATT_y
Cuts at: 369 2718 2974 369
Size: 2349 256 760
Fragments arranged by size:
2349 760 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3365
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3365
AvaI C'yCGr_G
Cuts at: 385 385
Size: 3365
BamHI G'GATC_C
Cuts at: 390 390
Size: 3365
BanI G'GyrC_C
Cuts at: 381 795 1043 1078 381
Size: 414 248 35 2668
Fragments arranged by size:
2668 414 248 35
BanII G_rGCy'C
Cuts at: 265 379 1409 265
Size: 114 1030 2221
Fragments arranged by size:
2221 1030 114
BbeI G_GCGC'C
Cuts at: 799 1047 799
Size: 248 3117
BciVI GTATCCnnnnn_n'
Cuts at: 1263 2024 3365 1263
Size: 761 1341 1263
Fragments arranged by size:
1341 1263 761
BclI T'GATC_A
Cuts at: 885 885
Size: 3365
BglII A'GATC_T
Cuts at: 880 2917 880
Size: 2037 1328
BlpI GC'TnA_GC
Cuts at: 477 477
Size: 3365
Bme1580I G_kGCm'C
Cuts at: 990 1083 2139 990
Size: 93 1056 2216
Fragments arranged by size:
2216 1056 93
BmgBI CAC'GTC
Cuts at: 301 301
Size: 3365
BmrI ACTGGGnnnn_n'
Cuts at: 577 658 993 3137 577
Size: 81 335 2144 805
Fragments arranged by size:
2144 805 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1732 1732
Size: 3365
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 304
Size: 3365
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 541 1420 1912 2210 319
Size: 222 879 492 298 1474
Fragments arranged by size:
1474 879 492 298 222
BsaI GGTCTCn'nnnn_
Cuts at: 3069 3069
Size: 3365
BsaAI yAC'GTr
Cuts at: 174 718 174
Size: 544 2821
BsaBI GATnn'nnATC
Cuts at: 6 879 2916 6
Size: 873 2037 455
Fragments arranged by size:
2037 873 455
BsaHI Gr'CG_yC
Cuts at: 796 1044 1746 3242 796
Size: 248 702 1496 919
Fragments arranged by size:
1496 919 702 248
BsaWI w'CCGG_w
Cuts at: 554 1075 1753 2027 2174 2585 554
Size: 521 678 274 147 411 1334
Fragments arranged by size:
1334 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 317 317
Size: 3365
BseYI C'CCAG_C
Cuts at: 790 1548 2125 2621 790
Size: 758 577 496 1534
Fragments arranged by size:
1534 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 907 953 2161 104
Size: 803 46 1208 1308
Fragments arranged by size:
1308 1208 803 46
BsiHKAI G_wGCw'C
Cuts at: 379 1157 1347 2139 379
Size: 778 190 792 1605
Fragments arranged by size:
1605 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 265 379 990 1083 1157 1347 1409 2139 265
Size: 114 611 93 74 190 62 730 1491
Fragments arranged by size:
1491 730 611 190 114 93 74 62
BspEI T'CCGG_A
Cuts at: 2585 2585
Size: 3365
BspHI T'CATG_A
Cuts at: 2541 2541
Size: 3365
BspMI ACCTGCnnnn'nnnn_
Cuts at: 415 629 931 1312 415
Size: 214 302 381 2468
Fragments arranged by size:
2468 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1657 1711 3195 3360 50
Size: 1607 54 1484 165 55
Fragments arranged by size:
1607 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1277 1277
Size: 3365
BsrFI r'CCGG_y
Cuts at: 164 1363 1544 1753 164
Size: 1199 181 209 1776
Fragments arranged by size:
1776 1199 209 181
BssSI C'ACGA_G
Cuts at: 1636 1994 1636
Size: 358 3007
BstAPI GCAn_nnn'nTGC
Cuts at: 204 742 204
Size: 538 2827
BstBI TT'CG_AA
Cuts at: 1726 1726
Size: 3365
BstYI r'GATC_y
Cuts at: 1 390 857 880 1215 1461 2473 2559 2571
Size: 389 467 23 335 246 1012 86 12
Cuts at: 2571 2917 1
Size: 346 449
Fragments arranged by size:
1012 467 449 389 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1315 1496 1659 1315
Size: 181 163 3021
Fragments arranged by size:
3021 181 163
BtsI GCAGTG_nn'
Cuts at: 149 608 149
Size: 459 2906
DraI TTT'AAA
Cuts at: 364 364
Size: 3365
DrdI GACnn_nn'nnGTC
Cuts at: 1071 1929 1071
Size: 858 2507
EaeI y'GGCC_r
Cuts at: 236 950 1124 1515 1542 2887 236
Size: 714 174 391 27 1345 714
Fragments arranged by size:
1345 714 714 391 174 27
EagI C'GGCC_G
Cuts at: 950 950
Size: 3365
EarI CTCTTCn'nnn_
Cuts at: 221 335 1388 1598 221
Size: 114 1053 210 1988
Fragments arranged by size:
1988 1053 210 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1573 1883 2029 3175 1573
Size: 310 146 1146 1763
Fragments arranged by size:
1763 1146 310 146
EcoICRI GAG'CTC
Cuts at: 377 377
Size: 3365
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 245 304 1190 1622 2369 2637 214
Size: 31 59 886 432 747 268 942
Fragments arranged by size:
942 886 747 432 268 59 31
EcoNI CCTnn'n_nnAGG
Cuts at: 286 286
Size: 3365
EcoO109I rG'GnC_Cy
Cuts at: 504 504
Size: 3365
EcoRI G'AATT_C
Cuts at: 369 369
Size: 3365
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1384 1384
Size: 3365
FspI TGC'GCA
Cuts at: 1146 1146
Size: 3365
HaeII r_GCGC'y
Cuts at: 799 1047 2069 3048 799
Size: 248 1022 979 1116
Fragments arranged by size:
1116 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1207 1237 1239 1269 1207
Size: 30 2 30 3303
Fragments arranged by size:
3303 30 30 2
HincII GTy'rAC
Cuts at: 404 404
Size: 3365
KasI G'GCGC_C
Cuts at: 795 1043 795
Size: 248 3117
KpnI G_GTAC'C
Cuts at: 385 385
Size: 3365
MluI A'CGCG_T
Cuts at: 2798 2798
Size: 3365
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2036 2220 2036
Size: 184 3181
MscI TGG'CCA
Cuts at: 1126 1126
Size: 3365
MslI CAynn'nnrTG
Cuts at: 1354 1481 1354
Size: 127 3238
MspA1I CmG'CkG
Cuts at: 476 790 1150 2163 2408 476
Size: 314 360 1013 245 1433
Fragments arranged by size:
1433 1013 360 314 245
NaeI GCC'GGC
Cuts at: 166 1546 166
Size: 1380 1985
NarI GG'CG_CC
Cuts at: 796 1044 796
Size: 248 3117
NgoMIV G'CCGG_C
Cuts at: 164 1544 164
Size: 1380 1985
NspI r_CATG'y
Cuts at: 418 918 1825 418
Size: 500 907 1958
Fragments arranged by size:
1958 907 500
PciI A'CATG_T
Cuts at: 1821 1821
Size: 3365
PmeI GTTT'AAAC
Cuts at: 364 364
Size: 3365
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2504 2536 2504
Size: 32 3333
PstI C_TGCA'G
Cuts at: 412 1097 412
Size: 685 2680
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3365
PvuII CAG'CTG
Cuts at: 790 1150 790
Size: 360 3005
RsrII CG'GwC_CG
Cuts at: 1560 1560
Size: 3365
SacI G_AGCT'C
Cuts at: 379 379
Size: 3365
SalI G'TCGA_C
Cuts at: 402 402
Size: 3365
SapI GCTCTTCn'nnn_
Cuts at: 1388 1598 1388
Size: 210 3155
SbfI CC_TGCA'GG
Cuts at: 412 412
Size: 3365
ScaI AGT'ACT
Cuts at: 2882 2882
Size: 3365
SfcI C'TryA_G
Cuts at: 33 408 1093 2086 2277 33
Size: 375 685 993 191 1121
Fragments arranged by size:
1121 993 685 375 191
SfoI GGC'GCC
Cuts at: 797 1045 797
Size: 248 3117
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3365
SmaI CCC'GGG
Cuts at: 387 387
Size: 3365
SmlI C'TyrA_G
Cuts at: 334 520 1435 1927 2189 334
Size: 186 915 492 262 1510
Fragments arranged by size:
1510 915 492 262 186
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3365
SphI G_CATG'C
Cuts at: 418 918 418
Size: 500 2865
StyI C'CwwG_G
Cuts at: 499 853 499
Size: 354 3011
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 948 1081 1540 1749 948
Size: 133 459 209 2564
Fragments arranged by size:
2564 459 209 133
TatI w'GTAC_w
Cuts at: 2880 2880
Size: 3365
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2882 2914 2882
Size: 32 3333
Tth111I GACn'n_nGTC
Cuts at: 1162 1162
Size: 3365
XbaI T'CTAG_A
Cuts at: 396 396
Size: 3365
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 860 860
Size: 3365
XmaI C'CCGG_G
Cuts at: 385 385
Size: 3365
XmnI GAAnn'nnTTC
Cuts at: 321 321
Size: 3365
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI