Restriction map for
pF1KB9671
(Circular) (Six-base) MAPSORT of: pF1KB9671.seq Check: 5391 from: 1 to: 4661
pF1KB9671 4661 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1699 1699
Size: 4661
Acc65I G'GTAC_C
Cuts at: 1677 1677
Size: 4661
AclI AA'CG_TT
Cuts at: 4501 4501
Size: 4661
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 984 1068 1152 1320 2486 2918 3665 3933
Size: 757 84 84 168 1166 432 747 268
Cuts at: 3933 227
Size: 955
Fragments arranged by size:
1166 955 757 747 432 268 168 84
84
AflIII A'CryG_T
Cuts at: 1648 2013 3117 4094 1648
Size: 365 1104 977 2215
Fragments arranged by size:
2215 1104 977 365
AgeI A'CCGG_T
Cuts at: 3049 3049
Size: 4661
AlwNI CAG_nnn'CTG
Cuts at: 1966 3533 1966
Size: 1567 3094
ApaLI G'TGCA_C
Cuts at: 3431 3431
Size: 4661
ApoI r'AATT_y
Cuts at: 530 671 698 755 1034 1260 1344 1370 1454
Size: 141 27 57 279 226 84 26 84
Cuts at: 1454 1665 4014 4270 530
Size: 211 2349 256 921
Fragments arranged by size:
2349 921 279 256 226 211 141 84
84 57 27 26
AseI AT'TA_AT
Cuts at: 20 20
Size: 4661
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4661
AvaI C'yCGr_G
Cuts at: 1681 1681
Size: 4661
BamHI G'GATC_C
Cuts at: 1686 1686
Size: 4661
BanI G'GyrC_C
Cuts at: 1677 2091 2339 2374 1677
Size: 414 248 35 3964
Fragments arranged by size:
3964 414 248 35
BanII G_rGCy'C
Cuts at: 1675 2705 1675
Size: 1030 3631
BbeI G_GCGC'C
Cuts at: 2095 2343 2095
Size: 248 4413
BbsI GAAGACnn'nnnn_
Cuts at: 570 738 906 990 1074 1158 1326 1410 570
Size: 168 168 84 84 84 168 84 3821
Fragments arranged by size:
3821 168 168 168 84 84 84 84
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4627
BciVI GTATCCnnnnn_n'
Cuts at: 2559 3320 4661 2559
Size: 761 1341 2559
Fragments arranged by size:
2559 1341 761
BclI T'GATC_A
Cuts at: 228 863 2181 228
Size: 635 1318 2708
Fragments arranged by size:
2708 1318 635
BfrBI ATG'CAT
Cuts at: 111 111
Size: 4661
BglII A'GATC_T
Cuts at: 491 2176 4213 491
Size: 1685 2037 939
Fragments arranged by size:
2037 1685 939
BlpI GC'TnA_GC
Cuts at: 1773 1773
Size: 4661
Bme1580I G_kGCm'C
Cuts at: 2286 2379 3435 2286
Size: 93 1056 3512
Fragments arranged by size:
3512 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1873 1954 2289 4433 1873
Size: 81 335 2144 2101
Fragments arranged by size:
2144 2101 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3028 3028
Size: 4661
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 560 644 812 896 980 1050 1064 1134 1148
Size: 84 168 84 84 70 14 70 14
Cuts at: 1148 1316 1400 1484 1568 560
Size: 168 84 84 84 3653
Fragments arranged by size:
3653 168 168 84 84 84 84 84
84 70 70 14 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1837 2716 3208 3506 1837
Size: 879 492 298 2992
Fragments arranged by size:
2992 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4365 4365
Size: 4661
BsaAI yAC'GTr
Cuts at: 2014 2014
Size: 4661
BsaBI GATnn'nnATC
Cuts at: 6 2175 4212 6
Size: 2169 2037 455
Fragments arranged by size:
2169 2037 455
BsaHI Gr'CG_yC
Cuts at: 2092 2340 3042 4538 2092
Size: 248 702 1496 2215
Fragments arranged by size:
2215 1496 702 248
BsaWI w'CCGG_w
Cuts at: 706 1850 2371 3049 3323 3470 3881 706
Size: 1144 521 678 274 147 411 1486
Fragments arranged by size:
1486 1144 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 2086 2844 3421 3917 2086
Size: 758 577 496 2830
Fragments arranged by size:
2830 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2203 2249 3457 104
Size: 2099 46 1208 1308
Fragments arranged by size:
2099 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1675 2453 2643 3435 1675
Size: 778 190 792 2901
Fragments arranged by size:
2901 792 778 190
BsmI GAATG_Cn'
Cuts at: 1249 1249
Size: 4661
BsmBI CGTCTCn'nnnn_
Cuts at: 454 454
Size: 4661
Bsp1286I G_dGCh'C
Cuts at: 1675 2286 2379 2453 2643 2705 3435 1675
Size: 611 93 74 190 62 730 2901
Fragments arranged by size:
2901 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3881 3881
Size: 4661
BspHI T'CATG_A
Cuts at: 3837 3837
Size: 4661
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1711 1925 2227 2608 1711
Size: 214 302 381 3764
Fragments arranged by size:
3764 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2953 3007 4491 4656 50
Size: 2903 54 1484 165 55
Fragments arranged by size:
2903 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2573 2573
Size: 4661
BsrFI r'CCGG_y
Cuts at: 1210 2659 2840 3049 1210
Size: 1449 181 209 2822
Fragments arranged by size:
2822 1449 209 181
BsrGI T'GTAC_A
Cuts at: 852 1272 852
Size: 420 4241
BssSI C'ACGA_G
Cuts at: 2932 3290 2932
Size: 358 4303
BstAPI GCAn_nnn'nTGC
Cuts at: 2038 2038
Size: 4661
BstBI TT'CG_AA
Cuts at: 3022 3022
Size: 4661
BstYI r'GATC_y
Cuts at: 1 491 1686 2153 2176 2511 2757 3769 3855
Size: 490 1195 467 23 335 246 1012 86
Cuts at: 3855 3867 4213 1
Size: 12 346 449
Fragments arranged by size:
1195 1012 490 467 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 592 2611 2792 2955 592
Size: 2019 181 163 2298
Fragments arranged by size:
2298 2019 181 163
BtsI GCAGTG_nn'
Cuts at: 1904 1904
Size: 4661
DraI TTT'AAA
Cuts at: 471 1660 471
Size: 1189 3472
DrdI GACnn_nn'nnGTC
Cuts at: 2367 3225 2367
Size: 858 3803
EaeI y'GGCC_r
Cuts at: 2246 2420 2811 2838 4183 2246
Size: 174 391 27 1345 2724
Fragments arranged by size:
2724 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2246 2246
Size: 4661
EarI CTCTTCn'nnn_
Cuts at: 1591 2684 2894 1591
Size: 1093 210 3358
Fragments arranged by size:
3358 1093 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2869 3179 3325 4471 2869
Size: 310 146 1146 3059
Fragments arranged by size:
3059 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1673 1673
Size: 4661
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 560 644 812 896 980 984 1050 1064
Size: 333 84 168 84 84 4 66 14
Cuts at: 1064 1068 1134 1148 1152 1316 1320 1400 1484
Size: 4 66 14 4 164 4 80 84
Cuts at: 1484 1568 2486 2918 3665 3933 227
Size: 84 918 432 747 268 955
Fragments arranged by size:
955 918 747 432 333 268 168 164
84 84 84 84 84 80 66 66
14 14 4 4 4 4
EcoO109I rG'GnC_Cy
Cuts at: 1800 1800
Size: 4661
EcoRI G'AATT_C
Cuts at: 530 698 1034 1370 1665 530
Size: 168 336 336 295 3526
Fragments arranged by size:
3526 336 336 295 168
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2680 2680
Size: 4661
FspI TGC'GCA
Cuts at: 2442 2442
Size: 4661
HaeII r_GCGC'y
Cuts at: 2095 2343 3365 4344 2095
Size: 248 1022 979 2412
Fragments arranged by size:
2412 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2503 2533 2535 2565 2503
Size: 30 2 30 4599
Fragments arranged by size:
4599 30 30 2
HincII GTy'rAC
Cuts at: 249 1700 249
Size: 1451 3210
HindIII A'AGCT_T
Cuts at: 1388 1499 1388
Size: 111 4550
KasI G'GCGC_C
Cuts at: 2091 2339 2091
Size: 248 4413
KpnI G_GTAC'C
Cuts at: 1681 1681
Size: 4661
MluI A'CGCG_T
Cuts at: 4094 4094
Size: 4661
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3332 3516 3332
Size: 184 4477
MscI TGG'CCA
Cuts at: 2422 2422
Size: 4661
MslI CAynn'nnrTG
Cuts at: 317 321 555 639 1211 2650 2777 317
Size: 4 234 84 572 1439 127 2201
Fragments arranged by size:
2201 1439 572 234 127 84 4
MspA1I CmG'CkG
Cuts at: 1772 2086 2446 3459 3704 1772
Size: 314 360 1013 245 2729
Fragments arranged by size:
2729 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2842 2842
Size: 4661
NarI GG'CG_CC
Cuts at: 2092 2340 2092
Size: 248 4413
NdeI CA'TA_TG
Cuts at: 318 318
Size: 4661
NgoMIV G'CCGG_C
Cuts at: 2840 2840
Size: 4661
NsiI A_TGCA'T
Cuts at: 113 113
Size: 4661
NspI r_CATG'y
Cuts at: 1652 1714 2214 3121 1652
Size: 62 500 907 3192
Fragments arranged by size:
3192 907 500 62
PciI A'CATG_T
Cuts at: 1648 3117 1648
Size: 1469 3192
PmeI GTTT'AAAC
Cuts at: 1660 1660
Size: 4661
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3800 3832 3800
Size: 32 4629
PsiI TTA'TAA
Cuts at: 722 1226 1394 722
Size: 504 168 3989
Fragments arranged by size:
3989 504 168
PstI C_TGCA'G
Cuts at: 194 1708 2393 194
Size: 1514 685 2462
Fragments arranged by size:
2462 1514 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4661
PvuII CAG'CTG
Cuts at: 2086 2446 2086
Size: 360 4301
RsrII CG'GwC_CG
Cuts at: 2856 2856
Size: 4661
SacI G_AGCT'C
Cuts at: 1675 1675
Size: 4661
SalI G'TCGA_C
Cuts at: 1698 1698
Size: 4661
SapI GCTCTTCn'nnn_
Cuts at: 2684 2894 2684
Size: 210 4451
SbfI CC_TGCA'GG
Cuts at: 1708 1708
Size: 4661
ScaI AGT'ACT
Cuts at: 4178 4178
Size: 4661
SfcI C'TryA_G
Cuts at: 33 190 1704 2389 3382 3573 33
Size: 157 1514 685 993 191 1121
Fragments arranged by size:
1514 1121 993 685 191 157
SfoI GGC'GCC
Cuts at: 2093 2341 2093
Size: 248 4413
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4661
SmaI CCC'GGG
Cuts at: 1683 1683
Size: 4661
SmlI C'TyrA_G
Cuts at: 1816 2731 3223 3485 1816
Size: 915 492 262 2992
Fragments arranged by size:
2992 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4661
SphI G_CATG'C
Cuts at: 1714 2214 1714
Size: 500 4161
SspI AAT'ATT
Cuts at: 369 369
Size: 4661
StuI AGG'CCT
Cuts at: 409 409
Size: 4661
StyI C'CwwG_G
Cuts at: 1795 2149 1795
Size: 354 4307
SwaI ATTT'AAAT
Cuts at: 471 471
Size: 4661
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1411 2244 2377 2836 3045 1411
Size: 833 133 459 209 3027
Fragments arranged by size:
3027 833 459 209 133
TatI w'GTAC_w
Cuts at: 852 1272 4176 852
Size: 420 2904 1337
Fragments arranged by size:
2904 1337 420
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 997 1029 1081 1113 4178 4210 997
Size: 32 52 32 3065 32 1448
Fragments arranged by size:
3065 1448 52 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2458 2458
Size: 4661
XbaI T'CTAG_A
Cuts at: 1692 1692
Size: 4661
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2156 2156
Size: 4661
XmaI C'CCGG_G
Cuts at: 1681 1681
Size: 4661
XmnI GAAnn'nnTTC
Cuts at: 1417 1474 1558 1417
Size: 57 84 4520
Fragments arranged by size:
4520 84 57
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NsiI
NspI PciI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BglI BmgBI BmtI
Bpu10I BsaXI BseRI BsgI BsiWI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NcoI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI XhoI ZraI