Restriction map for
pF1KB9660
(Circular) (Six-base) MAPSORT of: pF1KB9660.seq Check: 9491 from: 1 to: 4049
pF1KB9660 4049 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1087 1087
Size: 4049
Acc65I G'GTAC_C
Cuts at: 1065 1065
Size: 4049
AclI AA'CG_TT
Cuts at: 3889 3889
Size: 4049
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 534 786 805 870 1874 2306 3053 3321 534
Size: 252 19 65 1004 432 747 268 1262
Fragments arranged by size:
1262 1004 747 432 268 252 65 19
AflIII A'CryG_T
Cuts at: 274 1401 2505 3482 274
Size: 1127 1104 977 841
Fragments arranged by size:
1127 1104 977 841
AgeI A'CCGG_T
Cuts at: 2437 2437
Size: 4049
AleI CACnn'nnGTG
Cuts at: 569 569
Size: 4049
AlwNI CAG_nnn'CTG
Cuts at: 1354 2921 1354
Size: 1567 2482
ApaLI G'TGCA_C
Cuts at: 2819 2819
Size: 4049
ApoI r'AATT_y
Cuts at: 114 134 178 1053 3402 3658 114
Size: 20 44 875 2349 256 505
Fragments arranged by size:
2349 875 505 256 44 20
AseI AT'TA_AT
Cuts at: 20 20
Size: 4049
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
AvaI C'yCGr_G
Cuts at: 1069 1069
Size: 4049
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 798 831 798
Size: 33 4016
BamHI G'GATC_C
Cuts at: 1074 1074
Size: 4049
BanI G'GyrC_C
Cuts at: 1065 1479 1727 1762 1065
Size: 414 248 35 3352
Fragments arranged by size:
3352 414 248 35
BanII G_rGCy'C
Cuts at: 1063 2093 1063
Size: 1030 3019
BbeI G_GCGC'C
Cuts at: 1483 1731 1483
Size: 248 3801
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4015
BciVI GTATCCnnnnn_n'
Cuts at: 1947 2708 4049 1947
Size: 761 1341 1947
Fragments arranged by size:
1947 1341 761
BclI T'GATC_A
Cuts at: 1569 1569
Size: 4049
BfrBI ATG'CAT
Cuts at: 164 164
Size: 4049
BglII A'GATC_T
Cuts at: 1564 3601 1564
Size: 2037 2012
BlpI GC'TnA_GC
Cuts at: 1161 1161
Size: 4049
Bme1580I G_kGCm'C
Cuts at: 1674 1767 2823 1674
Size: 93 1056 2900
Fragments arranged by size:
2900 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 386 1261 1342 1677 3821 386
Size: 875 81 335 2144 614
Fragments arranged by size:
2144 875 614 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2416 2416
Size: 4049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 446 530 614 698 950 1034 446
Size: 84 84 84 252 84 3461
Fragments arranged by size:
3461 252 84 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1225 2104 2596 2894 1225
Size: 879 492 298 2380
Fragments arranged by size:
2380 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3753 3753
Size: 4049
BsaAI yAC'GTr
Cuts at: 1402 1402
Size: 4049
BsaBI GATnn'nnATC
Cuts at: 6 1563 3600 6
Size: 1557 2037 455
Fragments arranged by size:
2037 1557 455
BsaHI Gr'CG_yC
Cuts at: 1480 1728 2430 3926 1480
Size: 248 702 1496 1603
Fragments arranged by size:
1603 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1238 1759 2437 2711 2858 3269 1238
Size: 521 678 274 147 411 2018
Fragments arranged by size:
2018 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1474 2232 2809 3305 1474
Size: 758 577 496 2218
Fragments arranged by size:
2218 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1591 1637 2845 104
Size: 1487 46 1208 1308
Fragments arranged by size:
1487 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1063 1841 2031 2823 1063
Size: 778 190 792 2289
Fragments arranged by size:
2289 792 778 190
BsmI GAATG_Cn'
Cuts at: 166 259 166
Size: 93 3956
Bsp1286I G_dGCh'C
Cuts at: 1063 1674 1767 1841 2031 2093 2823 1063
Size: 611 93 74 190 62 730 2289
Fragments arranged by size:
2289 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3269 3269
Size: 4049
BspHI T'CATG_A
Cuts at: 3225 3225
Size: 4049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1099 1313 1615 1996 1099
Size: 214 302 381 3152
Fragments arranged by size:
3152 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2341 2395 3879 4044 50
Size: 2291 54 1484 165 55
Fragments arranged by size:
2291 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 541 793 1961 541
Size: 252 1168 2629
Fragments arranged by size:
2629 1168 252
BsrFI r'CCGG_y
Cuts at: 2047 2228 2437 2047
Size: 181 209 3659
Fragments arranged by size:
3659 209 181
BsrGI T'GTAC_A
Cuts at: 271 361 271
Size: 90 3959
BssSI C'ACGA_G
Cuts at: 829 2320 2678 829
Size: 1491 358 2200
Fragments arranged by size:
2200 1491 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1426 1426
Size: 4049
BstBI TT'CG_AA
Cuts at: 2410 2410
Size: 4049
BstYI r'GATC_y
Cuts at: 1 1074 1541 1564 1899 2145 3157 3243 3255
Size: 1073 467 23 335 246 1012 86 12
Cuts at: 3255 3601 1
Size: 346 449
Fragments arranged by size:
1073 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 465 465
Size: 4049
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1999 2180 2343 1999
Size: 181 163 3705
Fragments arranged by size:
3705 181 163
BtsI GCAGTG_nn'
Cuts at: 1292 1292
Size: 4049
DraI TTT'AAA
Cuts at: 1048 1048
Size: 4049
DrdI GACnn_nn'nnGTC
Cuts at: 1755 2613 1755
Size: 858 3191
EaeI y'GGCC_r
Cuts at: 1634 1808 2199 2226 3571 1634
Size: 174 391 27 1345 2112
Fragments arranged by size:
2112 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1634 1634
Size: 4049
EarI CTCTTCn'nnn_
Cuts at: 2072 2282 2072
Size: 210 3839
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2257 2567 2713 3859 2257
Size: 310 146 1146 2447
Fragments arranged by size:
2447 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1061 1061
Size: 4049
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 446 530 534 614 698 786 805 870 950
Size: 84 4 80 84 88 19 65 80
Cuts at: 950 1034 1874 2306 3053 3321 446
Size: 84 840 432 747 268 1174
Fragments arranged by size:
1174 840 747 432 268 88 84 84
84 80 80 65 19 4
EcoO109I rG'GnC_Cy
Cuts at: 1188 1188
Size: 4049
EcoRI G'AATT_C
Cuts at: 1053 1053
Size: 4049
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2068 2068
Size: 4049
FspI TGC'GCA
Cuts at: 1830 1830
Size: 4049
HaeII r_GCGC'y
Cuts at: 1483 1731 2753 3732 1483
Size: 248 1022 979 1800
Fragments arranged by size:
1800 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1891 1921 1923 1953 1891
Size: 30 2 30 3987
Fragments arranged by size:
3987 30 30 2
HincII GTy'rAC
Cuts at: 1088 1088
Size: 4049
HindIII A'AGCT_T
Cuts at: 713 713
Size: 4049
KasI G'GCGC_C
Cuts at: 1479 1727 1479
Size: 248 3801
KpnI G_GTAC'C
Cuts at: 1069 1069
Size: 4049
MfeI C'AATT_G
Cuts at: 645 870 645
Size: 225 3824
MluI A'CGCG_T
Cuts at: 3482 3482
Size: 4049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2720 2904 2720
Size: 184 3865
MscI TGG'CCA
Cuts at: 1810 1810
Size: 4049
MslI CAynn'nnrTG
Cuts at: 159 243 273 331 569 659 761 2038 2165
Size: 84 30 58 238 90 102 1277 127
Cuts at: 2165 159
Size: 2043
Fragments arranged by size:
2043 1277 238 127 102 90 84 58
30
MspA1I CmG'CkG
Cuts at: 1160 1474 1834 2847 3092 1160
Size: 314 360 1013 245 2117
Fragments arranged by size:
2117 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2230 2230
Size: 4049
NarI GG'CG_CC
Cuts at: 1480 1728 1480
Size: 248 3801
NdeI CA'TA_TG
Cuts at: 240 240
Size: 4049
NgoMIV G'CCGG_C
Cuts at: 2228 2228
Size: 4049
NsiI A_TGCA'T
Cuts at: 166 166
Size: 4049
NspI r_CATG'y
Cuts at: 278 1102 1602 2509 278
Size: 824 500 907 1818
Fragments arranged by size:
1818 907 824 500
PciI A'CATG_T
Cuts at: 274 2505 274
Size: 2231 1818
PmeI GTTT'AAAC
Cuts at: 1048 1048
Size: 4049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 4017
PstI C_TGCA'G
Cuts at: 1096 1781 1096
Size: 685 3364
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4049
PvuII CAG'CTG
Cuts at: 1474 1834 1474
Size: 360 3689
RsrII CG'GwC_CG
Cuts at: 2244 2244
Size: 4049
SacI G_AGCT'C
Cuts at: 1063 1063
Size: 4049
SalI G'TCGA_C
Cuts at: 1086 1086
Size: 4049
SapI GCTCTTCn'nnn_
Cuts at: 2072 2282 2072
Size: 210 3839
SbfI CC_TGCA'GG
Cuts at: 1096 1096
Size: 4049
ScaI AGT'ACT
Cuts at: 3566 3566
Size: 4049
SfcI C'TryA_G
Cuts at: 33 1092 1777 2770 2961 33
Size: 1059 685 993 191 1121
Fragments arranged by size:
1121 1059 993 685 191
SfoI GGC'GCC
Cuts at: 1481 1729 1481
Size: 248 3801
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
SmaI CCC'GGG
Cuts at: 1071 1071
Size: 4049
SmlI C'TyrA_G
Cuts at: 1204 2119 2611 2873 1204
Size: 915 492 262 2380
Fragments arranged by size:
2380 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4049
SphI G_CATG'C
Cuts at: 1102 1602 1102
Size: 500 3549
SspI AAT'ATT
Cuts at: 387 387
Size: 4049
StyI C'CwwG_G
Cuts at: 1183 1537 1183
Size: 354 3695
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 233 1632 1765 2224 2433 233
Size: 1399 133 459 209 1849
Fragments arranged by size:
1849 1399 459 209 133
TatI w'GTAC_w
Cuts at: 271 361 3564 271
Size: 90 3203 756
Fragments arranged by size:
3203 756 90
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3566 3598 3566
Size: 32 4017
Tth111I GACn'n_nGTC
Cuts at: 1846 1846
Size: 4049
XbaI T'CTAG_A
Cuts at: 1080 1080
Size: 4049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1544 1544
Size: 4049
XmaI C'CCGG_G
Cuts at: 1069 1069
Size: 4049
XmnI GAAnn'nnTTC
Cuts at: 547 631 799 547
Size: 84 168 3797
Fragments arranged by size:
3797 168 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NsiI NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BbsI BbvCI BglI BmgBI BmtI Bpu10I
BsaXI BseRI BsgI BsiWI BsmBI BssHII BstEII BstXI
BstZ17I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI NcoI NheI NotI NruI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI