Restriction map for
pF1KB9652
(Circular) (Six-base) MAPSORT of: pF1KB9652.seq Check: 5302 from: 1 to: 4064
pF1KB9652 4064 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1102 1102
Size: 4064
Acc65I G'GTAC_C
Cuts at: 1080 1080
Size: 4064
AclI AA'CG_TT
Cuts at: 3904 3904
Size: 4064
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 1889 2321 3068 3336 154
Size: 1735 432 747 268 882
Fragments arranged by size:
1735 882 747 432 268
AfeI AGC'GCT
Cuts at: 404 404
Size: 4064
AflIII A'CryG_T
Cuts at: 986 1416 2520 3497 986
Size: 430 1104 977 1553
Fragments arranged by size:
1553 1104 977 430
AgeI A'CCGG_T
Cuts at: 2452 2452
Size: 4064
AlwNI CAG_nnn'CTG
Cuts at: 1369 2936 1369
Size: 1567 2497
ApaI G_GGCC'C
Cuts at: 148 148
Size: 4064
ApaLI G'TGCA_C
Cuts at: 2834 2834
Size: 4064
ApoI r'AATT_y
Cuts at: 1068 3417 3673 1068
Size: 2349 256 1459
Fragments arranged by size:
2349 1459 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4064
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
AvaI C'yCGr_G
Cuts at: 657 1084 657
Size: 427 3637
BamHI G'GATC_C
Cuts at: 1089 1089
Size: 4064
BanI G'GyrC_C
Cuts at: 334 544 799 894 946 954 1014 1080 1494
Size: 210 255 95 52 8 60 66 414
Cuts at: 1494 1742 1777 334
Size: 248 35 2621
Fragments arranged by size:
2621 414 255 248 210 95 66 60
52 35 8
BanII G_rGCy'C
Cuts at: 148 1078 2108 148
Size: 930 1030 2104
Fragments arranged by size:
2104 1030 930
BbeI G_GCGC'C
Cuts at: 338 548 898 950 1498 1746 338
Size: 210 350 52 548 248 2656
Fragments arranged by size:
2656 548 350 248 210 52
BciVI GTATCCnnnnn_n'
Cuts at: 1962 2723 4064 1962
Size: 761 1341 1962
Fragments arranged by size:
1962 1341 761
BclI T'GATC_A
Cuts at: 926 1584 926
Size: 658 3406
BglI GCCn_nnn'nGGC
Cuts at: 495 495
Size: 4064
BglII A'GATC_T
Cuts at: 1579 3616 1579
Size: 2037 2027
BlpI GC'TnA_GC
Cuts at: 389 1176 389
Size: 787 3277
Bme1580I G_kGCm'C
Cuts at: 148 802 1019 1689 1782 2838 148
Size: 654 217 670 93 1056 1374
Fragments arranged by size:
1374 1056 670 654 217 93
BmgBI CAC'GTC
Cuts at: 711 711
Size: 4064
BmrI ACTGGGnnnn_n'
Cuts at: 1276 1357 1692 3836 1276
Size: 81 335 2144 1504
Fragments arranged by size:
2144 1504 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 870 2431 870
Size: 1561 2503
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 624 840 873 624
Size: 216 33 3815
Fragments arranged by size:
3815 216 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1240 2119 2611 2909 1240
Size: 879 492 298 2395
Fragments arranged by size:
2395 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3768 3768
Size: 4064
BsaAI yAC'GTr
Cuts at: 1417 1417
Size: 4064
BsaBI GATnn'nnATC
Cuts at: 6 1578 3615 6
Size: 1572 2037 455
Fragments arranged by size:
2037 1572 455
BsaHI Gr'CG_yC
Cuts at: 335 545 895 947 1495 1743 2445 3941 335
Size: 210 350 52 548 248 702 1496 458
Fragments arranged by size:
1496 702 548 458 350 248 210 52
BsaWI w'CCGG_w
Cuts at: 1253 1774 2452 2726 2873 3284 1253
Size: 521 678 274 147 411 2033
Fragments arranged by size:
2033 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 639 973 992 1489 2247 2824 3320 639
Size: 334 19 497 758 577 496 1383
Fragments arranged by size:
1383 758 577 497 496 334 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 391
Size: 4064
BsiEI CG_ry'CG
Cuts at: 104 197 373 1606 1652 2860 104
Size: 93 176 1233 46 1208 1308
Fragments arranged by size:
1308 1233 1208 176 93 46
BsiHKAI G_wGCw'C
Cuts at: 422 1078 1856 2046 2838 422
Size: 656 778 190 792 1648
Fragments arranged by size:
1648 792 778 656 190
BsmI GAATG_Cn'
Cuts at: 249 249
Size: 4064
BsmBI CGTCTCn'nnnn_
Cuts at: 118 352 118
Size: 234 3830
Bsp1286I G_dGCh'C
Cuts at: 148 422 802 1019 1078 1689 1782 1856 2046
Size: 274 380 217 59 611 93 74 190
Cuts at: 2046 2108 2838 148
Size: 62 730 1374
Fragments arranged by size:
1374 730 611 380 274 217 190 93
74 62 59
BspEI T'CCGG_A
Cuts at: 3284 3284
Size: 4064
BspHI T'CATG_A
Cuts at: 464 3240 464
Size: 2776 1288
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1114 1328 1630 2011 1114
Size: 214 302 381 3167
Fragments arranged by size:
3167 381 302 214
BsrBI CCG'CTC
Cuts at: 50 530 1007 2356 2410 3894 4059 50
Size: 480 477 1349 54 1484 165 55
Fragments arranged by size:
1484 1349 480 477 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1976 1976
Size: 4064
BsrFI r'CCGG_y
Cuts at: 196 437 547 2062 2243 2452 196
Size: 241 110 1515 181 209 1808
Fragments arranged by size:
1808 1515 241 209 181 110
BsrGI T'GTAC_A
Cuts at: 110 110
Size: 4064
BssSI C'ACGA_G
Cuts at: 2335 2693 2335
Size: 358 3706
BstAPI GCAn_nnn'nTGC
Cuts at: 696 1441 696
Size: 745 3319
BstBI TT'CG_AA
Cuts at: 2425 2425
Size: 4064
BstEII G'GTnAC_C
Cuts at: 870 870
Size: 4064
BstYI r'GATC_y
Cuts at: 1 1089 1556 1579 1914 2160 3172 3258 3270
Size: 1088 467 23 335 246 1012 86 12
Cuts at: 3270 3616 1
Size: 346 449
Fragments arranged by size:
1088 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 185 264 824 185
Size: 79 560 3425
Fragments arranged by size:
3425 560 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2014 2195 2358 2014
Size: 181 163 3720
Fragments arranged by size:
3720 181 163
BtsI GCAGTG_nn'
Cuts at: 1039 1307 1039
Size: 268 3796
DraI TTT'AAA
Cuts at: 1063 1063
Size: 4064
DrdI GACnn_nn'nnGTC
Cuts at: 1770 2628 1770
Size: 858 3206
EaeI y'GGCC_r
Cuts at: 194 370 1025 1649 1823 2214 2241 3586 194
Size: 176 655 624 174 391 27 1345 672
Fragments arranged by size:
1345 672 655 624 391 176 174 27
EagI C'GGCC_G
Cuts at: 194 370 1649 194
Size: 176 1279 2609
Fragments arranged by size:
2609 1279 176
EarI CTCTTCn'nnn_
Cuts at: 882 2087 2297 882
Size: 1205 210 2649
Fragments arranged by size:
2649 1205 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 462 2272 2582 2728 3874 462
Size: 1810 310 146 1146 652
Fragments arranged by size:
1810 1146 652 310 146
EcoICRI GAG'CTC
Cuts at: 1076 1076
Size: 4064
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 624 840 873 1889 2321 3068 3336 154
Size: 470 216 33 1016 432 747 268 882
Fragments arranged by size:
1016 882 747 470 432 268 216 33
EcoO109I rG'GnC_Cy
Cuts at: 912 1203 912
Size: 291 3773
EcoRI G'AATT_C
Cuts at: 1068 1068
Size: 4064
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 4064
FspI TGC'GCA
Cuts at: 671 1845 671
Size: 1174 2890
FspAI rTGC'GCAy
Cuts at: 671 671
Size: 4064
HaeII r_GCGC'y
Cuts at: 330 338 406 548 719 898 950 1498 1746
Size: 8 68 142 171 179 52 548 248
Cuts at: 1746 2768 3747 330
Size: 1022 979 647
Fragments arranged by size:
1022 979 647 548 248 179 171 142
68 52 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1906 1936 1938 1968 1906
Size: 30 2 30 4002
Fragments arranged by size:
4002 30 30 2
HincII GTy'rAC
Cuts at: 1103 1103
Size: 4064
KasI G'GCGC_C
Cuts at: 334 544 894 946 1494 1742 334
Size: 210 350 52 548 248 2656
Fragments arranged by size:
2656 548 350 248 210 52
KpnI G_GTAC'C
Cuts at: 1084 1084
Size: 4064
MluI A'CGCG_T
Cuts at: 3497 3497
Size: 4064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 335 2735 2919 335
Size: 2400 184 1480
Fragments arranged by size:
2400 1480 184
MscI TGG'CCA
Cuts at: 1825 1825
Size: 4064
MslI CAynn'nnrTG
Cuts at: 2053 2180 2053
Size: 127 3937
MspA1I CmG'CkG
Cuts at: 187 266 273 639 1175 1489 1849 2862 3107
Size: 79 7 366 536 314 360 1013 245
Cuts at: 3107 187
Size: 1144
Fragments arranged by size:
1144 1013 536 366 360 314 245 79
7
NaeI GCC'GGC
Cuts at: 198 549 2245 198
Size: 351 1696 2017
Fragments arranged by size:
2017 1696 351
NarI GG'CG_CC
Cuts at: 335 545 895 947 1495 1743 335
Size: 210 350 52 548 248 2656
Fragments arranged by size:
2656 548 350 248 210 52
NcoI C'CATG_G
Cuts at: 824 824
Size: 4064
NgoMIV G'CCGG_C
Cuts at: 196 547 2243 196
Size: 351 1696 2017
Fragments arranged by size:
2017 1696 351
NspI r_CATG'y
Cuts at: 783 936 990 1117 1617 2524 783
Size: 153 54 127 500 907 2323
Fragments arranged by size:
2323 907 500 153 127 54
PciI A'CATG_T
Cuts at: 986 2520 986
Size: 1534 2530
PflMI CCAn_nnn'nTGG
Cuts at: 576 576
Size: 4064
PfoI T'CCnGG_A
Cuts at: 917 917
Size: 4064
PmeI GTTT'AAAC
Cuts at: 1063 1063
Size: 4064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3203 3235 3203
Size: 32 4032
PpuMI rG'GwC_Cy
Cuts at: 912 912
Size: 4064
PsiI TTA'TAA
Cuts at: 431 431
Size: 4064
PspOMI G'GGCC_C
Cuts at: 144 144
Size: 4064
PstI C_TGCA'G
Cuts at: 725 1111 1796 725
Size: 386 685 2993
Fragments arranged by size:
2993 685 386
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4064
PvuII CAG'CTG
Cuts at: 639 1489 1849 639
Size: 850 360 2854
Fragments arranged by size:
2854 850 360
RsrII CG'GwC_CG
Cuts at: 222 2259 222
Size: 2037 2027
SacI G_AGCT'C
Cuts at: 1078 1078
Size: 4064
SacII CC_GC'GG
Cuts at: 188 267 188
Size: 79 3985
SalI G'TCGA_C
Cuts at: 1101 1101
Size: 4064
SapI GCTCTTCn'nnn_
Cuts at: 2087 2297 2087
Size: 210 3854
SbfI CC_TGCA'GG
Cuts at: 1111 1111
Size: 4064
ScaI AGT'ACT
Cuts at: 3581 3581
Size: 4064
SfcI C'TryA_G
Cuts at: 33 618 721 774 1107 1792 2785 2976 33
Size: 585 103 53 333 685 993 191 1121
Fragments arranged by size:
1121 993 685 585 333 191 103 53
SfoI GGC'GCC
Cuts at: 336 546 896 948 1496 1744 336
Size: 210 350 52 548 248 2656
Fragments arranged by size:
2656 548 350 248 210 52
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
SmaI CCC'GGG
Cuts at: 659 1086 659
Size: 427 3637
SmlI C'TyrA_G
Cuts at: 1219 2134 2626 2888 1219
Size: 915 492 262 2395
Fragments arranged by size:
2395 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4064
SphI G_CATG'C
Cuts at: 1117 1617 1117
Size: 500 3564
StyI C'CwwG_G
Cuts at: 824 1198 1552 824
Size: 374 354 3336
Fragments arranged by size:
3336 374 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1647 1780 2239 2448 1647
Size: 133 459 209 3263
Fragments arranged by size:
3263 459 209 133
TatI w'GTAC_w
Cuts at: 110 479 725 3579 110
Size: 369 246 2854 595
Fragments arranged by size:
2854 595 369 246
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3581 3613 3581
Size: 32 4032
Tth111I GACn'n_nGTC
Cuts at: 226 1861 226
Size: 1635 2429
XbaI T'CTAG_A
Cuts at: 1095 1095
Size: 4064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 831 864 1559 831
Size: 33 695 3336
Fragments arranged by size:
3336 695 33
XmaI C'CCGG_G
Cuts at: 657 1084 657
Size: 427 3637
XmnI GAAnn'nnTTC
Cuts at: 247 247
Size: 4064
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI Bpu10I
BsaXI BseRI BsiWI BssHII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI