Restriction map for
pF1KB9600
(Circular) (Six-base) MAPSORT of: pF1KB9600.seq Check: 4466 from: 1 to: 4232
pF1KB9600 4232 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 740 1270 740
Size: 530 3702
Acc65I G'GTAC_C
Cuts at: 1248 1248
Size: 4232
AclI AA'CG_TT
Cuts at: 4072 4072
Size: 4232
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2057 2489 3236 3504 2057
Size: 432 747 268 2785
Fragments arranged by size:
2785 747 432 268
AfeI AGC'GCT
Cuts at: 364 550 559 1112 364
Size: 186 9 553 3484
Fragments arranged by size:
3484 553 186 9
AflIII A'CryG_T
Cuts at: 512 1584 2688 3665 512
Size: 1072 1104 977 1079
Fragments arranged by size:
1104 1079 1072 977
AgeI A'CCGG_T
Cuts at: 2620 2620
Size: 4232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 550 550
Size: 4232
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 499 531 499
Size: 32 4200
AlwNI CAG_nnn'CTG
Cuts at: 1537 3104 1537
Size: 1567 2665
ApaI G_GGCC'C
Cuts at: 821 836 915 958 821
Size: 15 79 43 4095
Fragments arranged by size:
4095 79 43 15
ApaLI G'TGCA_C
Cuts at: 3002 3002
Size: 4232
ApoI r'AATT_y
Cuts at: 1236 3585 3841 1236
Size: 2349 256 1627
Fragments arranged by size:
2349 1627 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4232
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
AvaI C'yCGr_G
Cuts at: 316 468 684 1252 316
Size: 152 216 568 3296
Fragments arranged by size:
3296 568 216 152
BamHI G'GATC_C
Cuts at: 1257 1257
Size: 4232
BanI G'GyrC_C
Cuts at: 236 660 688 865 979 1162 1248 1662 1910
Size: 424 28 177 114 183 86 414 248
Cuts at: 1910 1945 236
Size: 35 2523
Fragments arranged by size:
2523 424 414 248 183 177 114 86
35 28
BanII G_rGCy'C
Cuts at: 821 836 915 958 1246 2276 821
Size: 15 79 43 288 1030 2777
Fragments arranged by size:
2777 1030 288 79 43 15
BbeI G_GCGC'C
Cuts at: 240 692 869 983 1166 1666 1914 240
Size: 452 177 114 183 500 248 2558
Fragments arranged by size:
2558 500 452 248 183 177 114
BbsI GAAGACnn'nnnn_
Cuts at: 768 768
Size: 4232
BciVI GTATCCnnnnn_n'
Cuts at: 2130 2891 4232 2130
Size: 761 1341 2130
Fragments arranged by size:
2130 1341 761
BclI T'GATC_A
Cuts at: 1752 1752
Size: 4232
BglI GCCn_nnn'nGGC
Cuts at: 919 940 962 919
Size: 21 22 4189
Fragments arranged by size:
4189 22 21
BglII A'GATC_T
Cuts at: 1747 3784 1747
Size: 2037 2195
BlpI GC'TnA_GC
Cuts at: 802 1344 802
Size: 542 3690
Bme1580I G_kGCm'C
Cuts at: 665 821 836 915 958 1857 1950 3006 665
Size: 156 15 79 43 899 93 1056 1891
Fragments arranged by size:
1891 1056 899 156 93 79 43 15
BmgBI CAC'GTC
Cuts at: 1091 1091
Size: 4232
BmrI ACTGGGnnnn_n'
Cuts at: 495 1444 1525 1860 4004 495
Size: 949 81 335 2144 723
Fragments arranged by size:
2144 949 723 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2599 2599
Size: 4232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 719 719
Size: 4232
Bpu10I CC'TnA_GC
Cuts at: 791 791
Size: 4232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 1408 2287 2779 3077 427
Size: 981 879 492 298 1582
Fragments arranged by size:
1582 981 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1057 3936 1057
Size: 2879 1353
BsaAI yAC'GTr
Cuts at: 1585 1585
Size: 4232
BsaBI GATnn'nnATC
Cuts at: 6 1746 3783 6
Size: 1740 2037 455
Fragments arranged by size:
2037 1740 455
BsaHI Gr'CG_yC
Cuts at: 237 689 754 866 980 1163 1663 1911 2613
Size: 452 65 112 114 183 500 248 702
Cuts at: 2613 4109 237
Size: 1496 360
Fragments arranged by size:
1496 702 500 452 360 248 183 114
112 65
BsaWI w'CCGG_w
Cuts at: 1421 1942 2620 2894 3041 3452 1421
Size: 521 678 274 147 411 2201
Fragments arranged by size:
2201 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 499 529 499
Size: 30 4202
BseYI C'CCAG_C
Cuts at: 333 813 1035 1113 1657 2415 2992 3488 333
Size: 480 222 78 544 758 577 496 1077
Fragments arranged by size:
1077 758 577 544 496 480 222 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 888 1178 888
Size: 290 3942
BsiEI CG_ry'CG
Cuts at: 104 465 799 919 1774 1820 3028 104
Size: 361 334 120 855 46 1208 1308
Fragments arranged by size:
1308 1208 855 361 334 120 46
BsiHKAI G_wGCw'C
Cuts at: 1246 2024 2214 3006 1246
Size: 778 190 792 2472
Fragments arranged by size:
2472 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 172 172
Size: 4232
Bsp1286I G_dGCh'C
Cuts at: 665 821 836 915 958 1246 1857 1950 2024
Size: 156 15 79 43 288 611 93 74
Cuts at: 2024 2214 2276 3006 665
Size: 190 62 730 1891
Fragments arranged by size:
1891 730 611 288 190 156 93 79
74 62 43 15
BspEI T'CCGG_A
Cuts at: 3452 3452
Size: 4232
BspHI T'CATG_A
Cuts at: 3408 3408
Size: 4232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1282 1496 1798 2179 1282
Size: 214 302 381 3335
Fragments arranged by size:
3335 381 302 214
BsrBI CCG'CTC
Cuts at: 50 122 524 795 801 2524 2578 4062 4227
Size: 72 402 271 6 1723 54 1484 165
Cuts at: 4227 50
Size: 55
Fragments arranged by size:
1723 1484 402 271 165 72 55 54
6
BsrDI GCAATG_nn'
Cuts at: 2144 2144
Size: 4232
BsrFI r'CCGG_y
Cuts at: 460 862 883 918 982 2230 2411 2620 460
Size: 402 21 35 64 1248 181 209 2072
Fragments arranged by size:
2072 1248 402 209 181 64 35 21
BssHII G'CGCG_C
Cuts at: 670 670
Size: 4232
BssSI C'ACGA_G
Cuts at: 2503 2861 2503
Size: 358 3874
BstAPI GCAn_nnn'nTGC
Cuts at: 1609 1609
Size: 4232
BstBI TT'CG_AA
Cuts at: 2593 2593
Size: 4232
BstYI r'GATC_y
Cuts at: 1 446 1257 1724 1747 2082 2328 3340 3426
Size: 445 811 467 23 335 246 1012 86
Cuts at: 3426 3438 3784 1
Size: 12 346 449
Fragments arranged by size:
1012 811 467 449 445 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 212 219 296 749 1067 1223 212
Size: 7 77 453 318 156 3221
Fragments arranged by size:
3221 453 318 156 77 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 267 276 288 2182 2363 2526 267
Size: 9 12 1894 181 163 1973
Fragments arranged by size:
1973 1894 181 163 12 9
BtsI GCAGTG_nn'
Cuts at: 993 1056 1475 993
Size: 63 419 3750
Fragments arranged by size:
3750 419 63
DraI TTT'AAA
Cuts at: 1231 1231
Size: 4232
DrdI GACnn_nn'nnGTC
Cuts at: 1938 2796 1938
Size: 858 3374
EaeI y'GGCC_r
Cuts at: 299 462 722 796 916 920 1008 1098 1817
Size: 163 260 74 120 4 88 90 719
Cuts at: 1817 1991 2382 2409 3754 299
Size: 174 391 27 1345 777
Fragments arranged by size:
1345 777 719 391 260 174 163 120
90 88 74 27 4
EagI C'GGCC_G
Cuts at: 462 796 916 1817 462
Size: 334 120 901 2877
Fragments arranged by size:
2877 901 334 120
EarI CTCTTCn'nnn_
Cuts at: 161 2255 2465 161
Size: 2094 210 1928
Fragments arranged by size:
2094 1928 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 400 849 921 1209 2440 2750 2896 4042 400
Size: 449 72 288 1231 310 146 1146 590
Fragments arranged by size:
1231 1146 590 449 310 288 146 72
EcoICRI GAG'CTC
Cuts at: 1244 1244
Size: 4232
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 719 2057 2489 3236 3504 719
Size: 1338 432 747 268 1447
Fragments arranged by size:
1447 1338 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 198 817 832 911 955 1021 1371 198
Size: 619 15 79 44 66 350 3059
Fragments arranged by size:
3059 619 350 79 66 44 15
EcoRI G'AATT_C
Cuts at: 1236 1236
Size: 4232
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2251 2251
Size: 4232
FseI GG_CCGG'CC
Cuts at: 464 922 464
Size: 458 3774
FspI TGC'GCA
Cuts at: 2013 2013
Size: 4232
HaeII r_GCGC'y
Cuts at: 240 249 324 366 493 552 561 692 869
Size: 9 75 42 127 59 9 131 177
Cuts at: 869 983 1114 1166 1666 1914 2936 3915 240
Size: 114 131 52 500 248 1022 979 557
Fragments arranged by size:
1022 979 557 500 248 177 131 131
127 114 75 59 52 42 9 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2074 2104 2106 2136 2074
Size: 30 2 30 4170
Fragments arranged by size:
4170 30 30 2
HincII GTy'rAC
Cuts at: 1271 1271
Size: 4232
KasI G'GCGC_C
Cuts at: 236 688 865 979 1162 1662 1910 236
Size: 452 177 114 183 500 248 2558
Fragments arranged by size:
2558 500 452 248 183 177 114
KpnI G_GTAC'C
Cuts at: 1252 1252
Size: 4232
MluI A'CGCG_T
Cuts at: 3665 3665
Size: 4232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2903 3087 2903
Size: 184 4048
MscI TGG'CCA
Cuts at: 301 1993 301
Size: 1692 2540
MslI CAynn'nnrTG
Cuts at: 295 2221 2348 295
Size: 1926 127 2179
Fragments arranged by size:
2179 1926 127
MspA1I CmG'CkG
Cuts at: 221 751 1003 1039 1343 1657 2017 3030 3275
Size: 530 252 36 304 314 360 1013 245
Cuts at: 3275 221
Size: 1178
Fragments arranged by size:
1178 1013 530 360 314 304 252 245
36
NaeI GCC'GGC
Cuts at: 462 864 920 984 2413 462
Size: 402 56 64 1429 2281
Fragments arranged by size:
2281 1429 402 64 56
NarI GG'CG_CC
Cuts at: 237 689 866 980 1163 1663 1911 237
Size: 452 177 114 183 500 248 2558
Fragments arranged by size:
2558 500 452 248 183 177 114
NcoI C'CATG_G
Cuts at: 296 1223 296
Size: 927 3305
NgoMIV G'CCGG_C
Cuts at: 460 862 918 982 2411 460
Size: 402 56 64 1429 2281
Fragments arranged by size:
2281 1429 402 64 56
NotI GC'GGCC_GC
Cuts at: 796 796
Size: 4232
NspI r_CATG'y
Cuts at: 516 591 1285 1785 2692 516
Size: 75 694 500 907 2056
Fragments arranged by size:
2056 907 694 500 75
PasI CC'CwG_GG
Cuts at: 202 202
Size: 4232
PciI A'CATG_T
Cuts at: 512 2688 512
Size: 2176 2056
PflMI CCAn_nnn'nTGG
Cuts at: 395 395
Size: 4232
PmeI GTTT'AAAC
Cuts at: 1231 1231
Size: 4232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 499 531 3371 3403 499
Size: 32 2840 32 1328
Fragments arranged by size:
2840 1328 32 32
PpuMI rG'GwC_Cy
Cuts at: 198 1021 198
Size: 823 3409
PspOMI G'GGCC_C
Cuts at: 817 832 911 954 817
Size: 15 79 43 4095
Fragments arranged by size:
4095 79 43 15
PstI C_TGCA'G
Cuts at: 257 1279 1964 257
Size: 1022 685 2525
Fragments arranged by size:
2525 1022 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4232
PvuII CAG'CTG
Cuts at: 1657 2017 1657
Size: 360 3872
RsrII CG'GwC_CG
Cuts at: 2427 2427
Size: 4232
SacI G_AGCT'C
Cuts at: 1246 1246
Size: 4232
SacII CC_GC'GG
Cuts at: 222 752 222
Size: 530 3702
SalI G'TCGA_C
Cuts at: 1269 1269
Size: 4232
SanDI GG'GwC_CC
Cuts at: 198 1021 198
Size: 823 3409
SapI GCTCTTCn'nnn_
Cuts at: 161 2255 2465 161
Size: 2094 210 1928
Fragments arranged by size:
2094 1928 210
SbfI CC_TGCA'GG
Cuts at: 1279 1279
Size: 4232
ScaI AGT'ACT
Cuts at: 3749 3749
Size: 4232
SfcI C'TryA_G
Cuts at: 33 253 273 1275 1960 2953 3144 33
Size: 220 20 1002 685 993 191 1121
Fragments arranged by size:
1121 1002 993 685 220 191 20
SfiI GGCCn_nnn'nGGCC
Cuts at: 919 919
Size: 4232
SfoI GGC'GCC
Cuts at: 238 690 867 981 1164 1664 1912 238
Size: 452 177 114 183 500 248 2558
Fragments arranged by size:
2558 500 452 248 183 177 114
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
SgrAI Cr'CCGG_yG
Cuts at: 862 862
Size: 4232
SmaI CCC'GGG
Cuts at: 470 686 1254 470
Size: 216 568 3448
Fragments arranged by size:
3448 568 216
SmlI C'TyrA_G
Cuts at: 442 1387 2302 2794 3056 442
Size: 945 915 492 262 1618
Fragments arranged by size:
1618 945 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4232
SphI G_CATG'C
Cuts at: 1285 1785 1285
Size: 500 3732
StyI C'CwwG_G
Cuts at: 296 1223 1366 1720 296
Size: 927 143 354 2808
Fragments arranged by size:
2808 927 354 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1815 1948 2407 2616 1815
Size: 133 459 209 3431
Fragments arranged by size:
3431 459 209 133
TatI w'GTAC_w
Cuts at: 3747 3747
Size: 4232
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3749 3781 3749
Size: 32 4200
Tth111I GACn'n_nGTC
Cuts at: 2029 2029
Size: 4232
XbaI T'CTAG_A
Cuts at: 1263 1263
Size: 4232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1727 1727
Size: 4232
XmaI C'CCGG_G
Cuts at: 468 684 1252 468
Size: 216 568 3448
Fragments arranged by size:
3448 568 216
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI BseRI BsiWI BsmI BsrGI
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FspAI HindIII HpaI MfeI NdeI NheI NruI
NsiI PacI PfoI PmlI PshAI PsiI PspXI PsrI
SexAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI