Restriction map for
pF1KB9546
(Circular) (Six-base) MAPSORT of: pF1KB9546.seq Check: 1695 from: 1 to: 4580
pF1KB9546 4580 bp
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1096 1096
Size: 4580
AccI GT'mk_AC
Cuts at: 1618 1618
Size: 4580
Acc65I G'GTAC_C
Cuts at: 1596 1596
Size: 4580
AclI AA'CG_TT
Cuts at: 1574 4420 1574
Size: 2846 1734
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2405 2837 3584 3852 2405
Size: 432 747 268 3133
Fragments arranged by size:
3133 747 432 268
AfeI AGC'GCT
Cuts at: 833 833
Size: 4580
AflIII A'CryG_T
Cuts at: 1932 3036 4013 1932
Size: 1104 977 2499
Fragments arranged by size:
2499 1104 977
AgeI A'CCGG_T
Cuts at: 2968 2968
Size: 4580
AleI CACnn'nnGTG
Cuts at: 247 610 247
Size: 363 4217
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1315 1315
Size: 4580
AlwNI CAG_nnn'CTG
Cuts at: 253 1278 1320 1885 3452 253
Size: 1025 42 565 1567 1381
Fragments arranged by size:
1567 1381 1025 565 42
ApaI G_GGCC'C
Cuts at: 1434 1514 1434
Size: 80 4500
ApaLI G'TGCA_C
Cuts at: 1228 3350 1228
Size: 2122 2458
ApoI r'AATT_y
Cuts at: 1584 3933 4189 1584
Size: 2349 256 1975
Fragments arranged by size:
2349 1975 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4580
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4580
AvaI C'yCGr_G
Cuts at: 127 363 706 808 1092 1600 127
Size: 236 343 102 284 508 3107
Fragments arranged by size:
3107 508 343 284 236 102
BamHI G'GATC_C
Cuts at: 1605 1605
Size: 4580
BanI G'GyrC_C
Cuts at: 1084 1137 1166 1402 1596 2010 2258 2293 1084
Size: 53 29 236 194 414 248 35 3371
Fragments arranged by size:
3371 414 248 236 194 53 35 29
BanII G_rGCy'C
Cuts at: 515 759 1434 1514 1594 2624 515
Size: 244 675 80 80 1030 2471
Fragments arranged by size:
2471 1030 675 244 80 80
BbeI G_GCGC'C
Cuts at: 1141 2014 2262 1141
Size: 873 248 3459
Fragments arranged by size:
3459 873 248
BbsI GAAGACnn'nnnn_
Cuts at: 788 1381 788
Size: 593 3987
BbvCI CC'TCA_GC
Cuts at: 1108 1108
Size: 4580
BciVI GTATCCnnnnn_n'
Cuts at: 2478 3239 4580 2478
Size: 761 1341 2478
Fragments arranged by size:
2478 1341 761
BclI T'GATC_A
Cuts at: 2100 2100
Size: 4580
BglI GCCn_nnn'nGGC
Cuts at: 595 1400 595
Size: 805 3775
BglII A'GATC_T
Cuts at: 2095 4132 2095
Size: 2037 2543
BlpI GC'TnA_GC
Cuts at: 214 535 1692 214
Size: 321 1157 3102
Fragments arranged by size:
3102 1157 321
Bme1580I G_kGCm'C
Cuts at: 1162 1205 1232 1405 1434 1514 2205 2298 3354
Size: 43 27 173 29 80 691 93 1056
Cuts at: 3354 1162
Size: 2388
Fragments arranged by size:
2388 1056 691 173 93 80 43 29
27
BmrI ACTGGGnnnn_n'
Cuts at: 1220 1401 1792 1873 2208 4352 1220
Size: 181 391 81 335 2144 1448
Fragments arranged by size:
2144 1448 391 335 181 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2947 2947
Size: 4580
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 952 1138 1342 1387 1450 952
Size: 186 204 45 63 4082
Fragments arranged by size:
4082 204 186 63 45
Bpu10I CC'TnA_GC
Cuts at: 758 1108 758
Size: 350 4230
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 674 1114 1756 2635 3127 3425 157
Size: 517 440 642 879 492 298 1312
Fragments arranged by size:
1312 879 642 517 492 440 298
BsaI GGTCTCn'nnnn_
Cuts at: 928 4284 928
Size: 3356 1224
BsaAI yAC'GTr
Cuts at: 1933 1933
Size: 4580
BsaBI GATnn'nnATC
Cuts at: 6 2094 4131 6
Size: 2088 2037 455
Fragments arranged by size:
2088 2037 455
BsaHI Gr'CG_yC
Cuts at: 1138 1345 2011 2259 2961 4457 1138
Size: 207 666 248 702 1496 1261
Fragments arranged by size:
1496 1261 702 666 248 207
BsaWI w'CCGG_w
Cuts at: 1769 2290 2968 3242 3389 3800 1769
Size: 521 678 274 147 411 2549
Fragments arranged by size:
2549 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 293 323 293
Size: 30 4550
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 640 754 1279 1339 640
Size: 114 525 60 3881
Fragments arranged by size:
3881 525 114 60
BseYI C'CCAG_C
Cuts at: 433 438 577 601 2005 2763 3340 3836 433
Size: 5 139 24 1404 758 577 496 1177
Fragments arranged by size:
1404 1177 758 577 496 139 24 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 715 1375 715
Size: 660 3920
BsiEI CG_ry'CG
Cuts at: 104 730 2122 2168 3376 104
Size: 626 1392 46 1208 1308
Fragments arranged by size:
1392 1308 1208 626 46
BsiHKAI G_wGCw'C
Cuts at: 715 759 1043 1232 1313 1594 2372 2562 3354
Size: 44 284 189 81 281 778 190 792
Cuts at: 3354 715
Size: 1941
Fragments arranged by size:
1941 792 778 284 281 190 189 81
44
Bsp1286I G_dGCh'C
Cuts at: 515 715 759 1043 1162 1205 1232 1313 1405
Size: 200 44 284 119 43 27 81 92
Cuts at: 1405 1434 1514 1594 2205 2298 2372 2562 2624
Size: 29 80 80 611 93 74 190 62
Cuts at: 2624 3354 515
Size: 730 1741
Fragments arranged by size:
1741 730 611 284 200 190 119 93
92 81 80 80 74 62 44 43
29 27
BspEI T'CCGG_A
Cuts at: 3800 3800
Size: 4580
BspHI T'CATG_A
Cuts at: 3756 3756
Size: 4580
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1079 1096 1630 1844 2146 2527 1079
Size: 17 534 214 302 381 3132
Fragments arranged by size:
3132 534 381 302 214 17
BsrBI CCG'CTC
Cuts at: 50 304 888 1329 2872 2926 4410 4575 50
Size: 254 584 441 1543 54 1484 165 55
Fragments arranged by size:
1543 1484 584 441 254 165 55 54
BsrDI GCAATG_nn'
Cuts at: 588 2492 588
Size: 1904 2676
BsrFI r'CCGG_y
Cuts at: 122 374 785 1334 2578 2759 2968 122
Size: 252 411 549 1244 181 209 1734
Fragments arranged by size:
1734 1244 549 411 252 209 181
BssHII G'CGCG_C
Cuts at: 151 151
Size: 4580
BssSI C'ACGA_G
Cuts at: 2851 3209 2851
Size: 358 4222
BstAPI GCAn_nnn'nTGC
Cuts at: 1278 1957 1278
Size: 679 3901
BstBI TT'CG_AA
Cuts at: 2941 2941
Size: 4580
BstEII G'GTnAC_C
Cuts at: 843 1470 843
Size: 627 3953
BstYI r'GATC_y
Cuts at: 1 1605 2072 2095 2430 2676 3688 3774 3786
Size: 1604 467 23 335 246 1012 86 12
Cuts at: 3786 4132 1
Size: 346 449
Fragments arranged by size:
1604 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 266 317 266
Size: 51 4529
BtgI C'CryG_G
Cuts at: 107 140 199 413 593 848 107
Size: 33 59 214 180 255 3839
Fragments arranged by size:
3839 255 214 180 59 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2530 2711 2874 2530
Size: 181 163 4236
Fragments arranged by size:
4236 181 163
BtsI GCAGTG_nn'
Cuts at: 242 254 314 546 1299 1358 1498 1823 242
Size: 12 60 232 753 59 140 325 2999
Fragments arranged by size:
2999 753 325 232 140 60 59 12
DraI TTT'AAA
Cuts at: 1579 1579
Size: 4580
DrdI GACnn_nn'nnGTC
Cuts at: 670 2286 3144 670
Size: 1616 858 2106
Fragments arranged by size:
2106 1616 858
EaeI y'GGCC_r
Cuts at: 290 376 727 1220 1505 2165 2339 2730 2757
Size: 86 351 493 285 660 174 391 27
Cuts at: 2757 4102 290
Size: 1345 768
Fragments arranged by size:
1345 768 660 493 391 351 285 174
86 27
EagI C'GGCC_G
Cuts at: 727 2165 727
Size: 1438 3142
EarI CTCTTCn'nnn_
Cuts at: 886 1256 2603 2813 886
Size: 370 1347 210 2653
Fragments arranged by size:
2653 1347 370 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2788 3098 3244 4390 2788
Size: 310 146 1146 2978
Fragments arranged by size:
2978 1146 310 146
EcoICRI GAG'CTC
Cuts at: 757 1592 757
Size: 835 3745
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 952 1138 1342 1387 1450 2405 2837 3584 3852
Size: 186 204 45 63 955 432 747 268
Cuts at: 3852 952
Size: 1680
Fragments arranged by size:
1680 955 747 432 268 204 186 63
45
EcoNI CCTnn'n_nnAGG
Cuts at: 282 282
Size: 4580
EcoO109I rG'GnC_Cy
Cuts at: 262 501 1005 1097 1431 1511 1719 262
Size: 239 504 92 334 80 208 3123
Fragments arranged by size:
3123 504 334 239 208 92 80
EcoRI G'AATT_C
Cuts at: 1584 1584
Size: 4580
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2599 2599
Size: 4580
FspI TGC'GCA
Cuts at: 2361 2361
Size: 4580
HaeII r_GCGC'y
Cuts at: 136 484 835 1141 2014 2262 3284 4263 136
Size: 348 351 306 873 248 1022 979 453
Fragments arranged by size:
1022 979 873 453 351 348 306 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 730 762 2422 2452 2454 2484 730
Size: 32 1660 30 2 30 2826
Fragments arranged by size:
2826 1660 32 30 30 2
HincII GTy'rAC
Cuts at: 1619 1619
Size: 4580
HindIII A'AGCT_T
Cuts at: 227 227
Size: 4580
KasI G'GCGC_C
Cuts at: 1137 2010 2258 1137
Size: 873 248 3459
Fragments arranged by size:
3459 873 248
KpnI G_GTAC'C
Cuts at: 1600 1600
Size: 4580
MluI A'CGCG_T
Cuts at: 4013 4013
Size: 4580
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 983 3251 3435 983
Size: 2268 184 2128
Fragments arranged by size:
2268 2128 184
MscI TGG'CCA
Cuts at: 1222 2341 1222
Size: 1119 3461
MslI CAynn'nnrTG
Cuts at: 247 610 1235 2569 2696 247
Size: 363 625 1334 127 2131
Fragments arranged by size:
2131 1334 625 363 127
MspA1I CmG'CkG
Cuts at: 201 534 923 1112 1320 1461 1691 2005 2365
Size: 333 389 189 208 141 230 314 360
Cuts at: 2365 3378 3623 201
Size: 1013 245 1158
Fragments arranged by size:
1158 1013 389 360 333 314 245 230
208 189 141
NaeI GCC'GGC
Cuts at: 124 2761 124
Size: 2637 1943
NarI GG'CG_CC
Cuts at: 1138 2011 2259 1138
Size: 873 248 3459
Fragments arranged by size:
3459 873 248
NcoI C'CATG_G
Cuts at: 107 593 107
Size: 486 4094
NgoMIV G'CCGG_C
Cuts at: 122 2759 122
Size: 2637 1943
NotI GC'GGCC_GC
Cuts at: 727 727
Size: 4580
NspI r_CATG'y
Cuts at: 1633 2133 3040 1633
Size: 500 907 3173
Fragments arranged by size:
3173 907 500
PasI CC'CwG_GG
Cuts at: 1563 1563
Size: 4580
PciI A'CATG_T
Cuts at: 3036 3036
Size: 4580
PflMI CCAn_nnn'nTGG
Cuts at: 1320 1320
Size: 4580
PfoI T'CCnGG_A
Cuts at: 929 929
Size: 4580
PmeI GTTT'AAAC
Cuts at: 1579 1579
Size: 4580
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 3719
Size: 32 4548
PpuMI rG'GwC_Cy
Cuts at: 262 1005 262
Size: 743 3837
PspOMI G'GGCC_C
Cuts at: 1430 1510 1430
Size: 80 4500
PstI C_TGCA'G
Cuts at: 119 249 1076 1304 1627 2312 119
Size: 130 827 228 323 685 2387
Fragments arranged by size:
2387 827 685 323 228 130
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4580
PvuII CAG'CTG
Cuts at: 1112 1320 1461 2005 2365 1112
Size: 208 141 544 360 3327
Fragments arranged by size:
3327 544 360 208 141
RsrII CG'GwC_CG
Cuts at: 2775 2775
Size: 4580
SacI G_AGCT'C
Cuts at: 759 1594 759
Size: 835 3745
SacII CC_GC'GG
Cuts at: 202 202
Size: 4580
SalI G'TCGA_C
Cuts at: 1617 1617
Size: 4580
SapI GCTCTTCn'nnn_
Cuts at: 2603 2813 2603
Size: 210 4370
SbfI CC_TGCA'GG
Cuts at: 1627 1627
Size: 4580
ScaI AGT'ACT
Cuts at: 4097 4097
Size: 4580
SfcI C'TryA_G
Cuts at: 33 115 245 918 1072 1300 1623 2308 3301
Size: 82 130 673 154 228 323 685 993
Cuts at: 3301 3492 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 673 323 228 191 154
130 82
SfoI GGC'GCC
Cuts at: 1139 2012 2260 1139
Size: 873 248 3459
Fragments arranged by size:
3459 873 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4580
SmaI CCC'GGG
Cuts at: 365 1094 1602 365
Size: 729 508 3343
Fragments arranged by size:
3343 729 508
SmlI C'TyrA_G
Cuts at: 172 689 1129 1735 2650 3142 3404 172
Size: 517 440 606 915 492 262 1348
Fragments arranged by size:
1348 915 606 517 492 440 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4580
SphI G_CATG'C
Cuts at: 1633 2133 1633
Size: 500 4080
StuI AGG'CCT
Cuts at: 839 1180 839
Size: 341 4239
StyI C'CwwG_G
Cuts at: 107 593 1142 1714 2068 107
Size: 486 549 572 354 2619
Fragments arranged by size:
2619 572 549 486 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 396 952 1045 1143 1258 1420 2163 2296 2755
Size: 556 93 98 115 162 743 133 459
Cuts at: 2755 2964 396
Size: 209 2012
Fragments arranged by size:
2012 743 556 459 209 162 133 115
98 93
TatI w'GTAC_w
Cuts at: 4095 4095
Size: 4580
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 293 325 4097 4129 293
Size: 32 3772 32 744
Fragments arranged by size:
3772 744 32 32
Tth111I GACn'n_nGTC
Cuts at: 2377 2377
Size: 4580
XbaI T'CTAG_A
Cuts at: 1611 1611
Size: 4580
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 855 1527 2075 855
Size: 672 548 3360
Fragments arranged by size:
3360 672 548
XmaI C'CCGG_G
Cuts at: 363 1092 1600 363
Size: 729 508 3343
Fragments arranged by size:
3343 729 508
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BcgI
BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI BstXI
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NdeI NheI NruI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI