Restriction map for
pF1KB9389
(Circular) (Six-base) MAPSORT of: pF1KB9389.seq Check: 3925 from: 1 to: 5198
pF1KB9389 5198 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 637 1311 2236 637
Size: 674 925 3599
Fragments arranged by size:
3599 925 674
Acc65I G'GTAC_C
Cuts at: 2214 2214
Size: 5198
AclI AA'CG_TT
Cuts at: 5038 5038
Size: 5198
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 629 638 1157 1709 3023 3455 3808 4470 629
Size: 9 519 552 1314 432 353 662 1357
Fragments arranged by size:
1357 1314 662 552 519 432 353 9
AflIII A'CryG_T
Cuts at: 2550 4350 4631 2550
Size: 1800 281 3117
Fragments arranged by size:
3117 1800 281
AgeI A'CCGG_T
Cuts at: 1199 1199
Size: 5198
AhdI GACnn_n'nnGTC
Cuts at: 771 771
Size: 5198
AlwNI CAG_nnn'CTG
Cuts at: 211 808 982 1135 1273 2503 3941 211
Size: 597 174 153 138 1230 1438 1468
Fragments arranged by size:
1468 1438 1230 597 174 153 138
ApaLI G'TGCA_C
Cuts at: 4036 4036
Size: 5198
ApoI r'AATT_y
Cuts at: 1654 2202 4551 4807 1654
Size: 548 2349 256 2045
Fragments arranged by size:
2349 2045 548 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5198
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5198
AvaI C'yCGr_G
Cuts at: 940 2218 940
Size: 1278 3920
BamHI G'GATC_C
Cuts at: 2223 2223
Size: 5198
BanI G'GyrC_C
Cuts at: 964 2214 2628 2876 2911 964
Size: 1250 414 248 35 3251
Fragments arranged by size:
3251 1250 414 248 35
BanII G_rGCy'C
Cuts at: 989 1734 2212 3242 989
Size: 745 478 1030 2945
Fragments arranged by size:
2945 1030 745 478
BbeI G_GCGC'C
Cuts at: 2632 2880 2632
Size: 248 4950
BbsI GAAGACnn'nnnn_
Cuts at: 1469 1598 1469
Size: 129 5069
BciVI GTATCCnnnnn_n'
Cuts at: 912 3096 4152 5198 912
Size: 2184 1056 1046 912
Fragments arranged by size:
2184 1056 1046 912
BclI T'GATC_A
Cuts at: 930 2718 930
Size: 1788 3410
BglII A'GATC_T
Cuts at: 2037 2713 4750 2037
Size: 676 2037 2485
Fragments arranged by size:
2485 2037 676
BlpI GC'TnA_GC
Cuts at: 2310 2310
Size: 5198
Bme1580I G_kGCm'C
Cuts at: 967 1351 2823 2916 4040 967
Size: 384 1472 93 1124 2125
Fragments arranged by size:
2125 1472 1124 384 93
BmrI ACTGGGnnnn_n'
Cuts at: 970 2410 2491 2826 4970 970
Size: 1440 81 335 2144 1198
Fragments arranged by size:
2144 1440 1198 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1641 3565 1641
Size: 1924 3274
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1250 1748 1250
Size: 498 4700
Bpu10I CC'TnA_GC
Cuts at: 983 1328 983
Size: 345 4853
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2374 3253 3967 4265 2374
Size: 879 714 298 3307
Fragments arranged by size:
3307 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4902 4902
Size: 5198
BsaAI yAC'GTr
Cuts at: 2551 2551
Size: 5198
BsaBI GATnn'nnATC
Cuts at: 6 2712 4749 6
Size: 2706 2037 455
Fragments arranged by size:
2706 2037 455
BsaHI Gr'CG_yC
Cuts at: 767 2629 2877 3579 5075 767
Size: 1862 248 702 1496 890
Fragments arranged by size:
1862 1496 890 702 248
BsaWI w'CCGG_w
Cuts at: 537 1199 2387 2908 3586 3997 4144 4418 537
Size: 662 1188 521 678 411 147 274 1317
Fragments arranged by size:
1317 1188 678 662 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 431 461 696 726 1273 1303 1485 1515 431
Size: 30 235 30 547 30 182 30 4114
Fragments arranged by size:
4114 547 235 182 30 30 30 30
BseYI C'CCAG_C
Cuts at: 513 1707 2623 3381 4046 4454 513
Size: 1194 916 758 665 408 1257
Fragments arranged by size:
1257 1194 916 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2740 2786 4016 104
Size: 2636 46 1230 1286
Fragments arranged by size:
2636 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 989 2212 2990 3180 4040 989
Size: 1223 778 190 860 2147
Fragments arranged by size:
2147 1223 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 967 989 1351 1734 2212 2823 2916 2990 3180
Size: 22 362 383 478 611 93 74 190
Cuts at: 3180 3242 4040 967
Size: 62 798 2125
Fragments arranged by size:
2125 798 611 478 383 362 190 93
74 62 22
BspHI T'CATG_A
Cuts at: 249 3630 249
Size: 3381 1817
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2007 2248 2462 2764 3145 2007
Size: 241 214 302 381 4060
Fragments arranged by size:
4060 381 302 241 214
BsrBI CCG'CTC
Cuts at: 50 998 3490 3544 5028 5193 50
Size: 948 2492 54 1484 165 55
Fragments arranged by size:
2492 1484 948 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3110 3110
Size: 5198
BsrFI r'CCGG_y
Cuts at: 1199 1332 3196 3377 1199
Size: 133 1864 181 3020
Fragments arranged by size:
3020 1864 181 133
BssSI C'ACGA_G
Cuts at: 3469 4177 3469
Size: 708 4490
BstAPI GCAn_nnn'nTGC
Cuts at: 2575 2575
Size: 5198
BstBI TT'CG_AA
Cuts at: 3559 3559
Size: 5198
BstYI r'GATC_y
Cuts at: 1 657 1407 2037 2223 2690 2713 3048 3294
Size: 656 750 630 186 467 23 335 246
Cuts at: 3294 3600 3612 3698 4750 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 750 656 630 467 449 335 306
246 186 86 23 12
BstZ17I GTA'TAC
Cuts at: 638 1312 638
Size: 674 4524
BtgI C'CryG_G
Cuts at: 1100 1100
Size: 5198
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3148 3329 3492 3148
Size: 181 163 4854
Fragments arranged by size:
4854 181 163
BtsI GCAGTG_nn'
Cuts at: 458 2441 458
Size: 1983 3215
DraI TTT'AAA
Cuts at: 848 2197 848
Size: 1349 3849
DraIII CAC_nnn'GTG
Cuts at: 1741 1741
Size: 5198
DrdI GACnn_nn'nnGTC
Cuts at: 2904 4248 2904
Size: 1344 3854
EaeI y'GGCC_r
Cuts at: 2783 2957 3348 3375 4720 2783
Size: 174 391 27 1345 3261
Fragments arranged by size:
3261 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2783 2783
Size: 5198
EarI CTCTTCn'nnn_
Cuts at: 1514 3221 3431 1514
Size: 1707 210 3281
Fragments arranged by size:
3281 1707 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3406 4148 4294 5008 3406
Size: 742 146 714 3596
Fragments arranged by size:
3596 742 714 146
EcoICRI GAG'CTC
Cuts at: 987 2210 987
Size: 1223 3975
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 629 638 1157 1250 1709 1748 3023 3455 3808
Size: 9 519 93 459 39 1275 432 353
Cuts at: 3808 4470 629
Size: 662 1357
Fragments arranged by size:
1357 1275 662 519 459 432 353 93
39 9
EcoO109I rG'GnC_Cy
Cuts at: 604 2337 604
Size: 1733 3465
EcoRI G'AATT_C
Cuts at: 2202 2202
Size: 5198
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1615 3217 1615
Size: 1602 3596
FspI TGC'GCA
Cuts at: 2979 2979
Size: 5198
HaeII r_GCGC'y
Cuts at: 2632 2880 4110 4881 2632
Size: 248 1230 771 2949
Fragments arranged by size:
2949 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 205 237 244 276 532 564 757 789 934
Size: 32 7 32 256 32 193 32 145
Cuts at: 934 966 982 1014 1207 1239 1273 1305 1597
Size: 32 16 32 193 32 34 32 292
Cuts at: 1597 1629 1774 1806 2014 2046 3040 3070 3072
Size: 32 145 32 208 32 994 30 2
Cuts at: 3072 3102 205
Size: 30 2301
Fragments arranged by size:
2301 994 292 256 208 193 193 145
145 34 32 32 32 32 32 32
32 32 32 32 32 30 30 16
7 2
HincII GTy'rAC
Cuts at: 230 2237 230
Size: 2007 3191
HindIII A'AGCT_T
Cuts at: 1474 1885 1474
Size: 411 4787
KasI G'GCGC_C
Cuts at: 2628 2876 2628
Size: 248 4950
KpnI G_GTAC'C
Cuts at: 2218 2218
Size: 5198
MluI A'CGCG_T
Cuts at: 4631 4631
Size: 5198
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1259 3957 4141 1259
Size: 2698 184 2316
Fragments arranged by size:
2698 2316 184
MscI TGG'CCA
Cuts at: 2959 2959
Size: 5198
MslI CAynn'nnrTG
Cuts at: 3187 3314 3187
Size: 127 5071
MspA1I CmG'CkG
Cuts at: 370 517 805 1261 2048 2309 2623 2983 3767
Size: 147 288 456 787 261 314 360 784
Cuts at: 3767 4012 370
Size: 245 1556
Fragments arranged by size:
1556 787 784 456 360 314 288 261
245 147
NaeI GCC'GGC
Cuts at: 1334 3379 1334
Size: 2045 3153
NarI GG'CG_CC
Cuts at: 2629 2877 2629
Size: 248 4950
NgoMIV G'CCGG_C
Cuts at: 1332 3377 1332
Size: 2045 3153
NspI r_CATG'y
Cuts at: 2251 2751 4354 2251
Size: 500 1603 3095
Fragments arranged by size:
3095 1603 500
PciI A'CATG_T
Cuts at: 4350 4350
Size: 5198
PflMI CCAn_nnn'nTGG
Cuts at: 150 1417 150
Size: 1267 3931
PfoI T'CCnGG_A
Cuts at: 1931 1931
Size: 5198
PmeI GTTT'AAAC
Cuts at: 2197 2197
Size: 5198
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 971 1003 3644 3676 971
Size: 32 2641 32 2493
Fragments arranged by size:
2641 2493 32 32
PpuMI rG'GwC_Cy
Cuts at: 604 604
Size: 5198
PshAI GACnn'nnGTC
Cuts at: 2048 2048
Size: 5198
PspXI vC'TCGA_Gb
Cuts at: 940 940
Size: 5198
PstI C_TGCA'G
Cuts at: 2004 2245 2930 2004
Size: 241 685 4272
Fragments arranged by size:
4272 685 241
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5198
PvuII CAG'CTG
Cuts at: 370 517 805 2048 2623 2983 370
Size: 147 288 1243 575 360 2585
Fragments arranged by size:
2585 1243 575 360 288 147
RsrII CG'GwC_CG
Cuts at: 3393 3393
Size: 5198
SacI G_AGCT'C
Cuts at: 989 2212 989
Size: 1223 3975
SalI G'TCGA_C
Cuts at: 2235 2235
Size: 5198
SapI GCTCTTCn'nnn_
Cuts at: 3221 3431 3221
Size: 210 4988
SbfI CC_TGCA'GG
Cuts at: 2245 2245
Size: 5198
ScaI AGT'ACT
Cuts at: 4715 4715
Size: 5198
SfcI C'TryA_G
Cuts at: 33 761 800 836 1367 2000 2060 2241 2926
Size: 728 39 36 531 633 60 181 685
Cuts at: 2926 3894 4085 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 728 685 633 531 191 181
60 39 36
SfoI GGC'GCC
Cuts at: 2630 2878 2630
Size: 248 4950
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5198
SmaI CCC'GGG
Cuts at: 2220 2220
Size: 5198
SmlI C'TyrA_G
Cuts at: 940 2353 3268 3982 4244 940
Size: 1413 915 714 262 1894
Fragments arranged by size:
1894 1413 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5198
SphI G_CATG'C
Cuts at: 2251 2751 2251
Size: 500 4698
StyI C'CwwG_G
Cuts at: 125 1376 1778 2332 2686 125
Size: 1251 402 554 354 2637
Fragments arranged by size:
2637 1251 554 402 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1596 2781 2914 3373 3582 1596
Size: 1185 133 459 209 3212
Fragments arranged by size:
3212 1185 459 209 133
TatI w'GTAC_w
Cuts at: 1082 4713 1082
Size: 3631 1567
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 696 728 1412 1444 4715 4747 696
Size: 32 684 32 3271 32 1147
Fragments arranged by size:
3271 1147 684 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2995 2995
Size: 5198
XbaI T'CTAG_A
Cuts at: 2229 2229
Size: 5198
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 240 1689 2693 240
Size: 1449 1004 2745
Fragments arranged by size:
2745 1449 1004
XhoI C'TCGA_G
Cuts at: 940 940
Size: 5198
XmaI C'CCGG_G
Cuts at: 2218 2218
Size: 5198
XmnI GAAnn'nnTTC
Cuts at: 1496 1574 1496
Size: 78 5120
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BseRI BsgI BsiWI BsmI BsmBI BspEI BsrGI
BssHII BstEII BstXI Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PsiI PspOMI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI ZraI