Restriction map for
pF1KB9387
(Circular) (Six-base) MAPSORT of: pF1KB9387.seq Check: 2749 from: 1 to: 3977
pF1KB9387 3977 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 252 252
Size: 3977
AccI GT'mk_AC
Cuts at: 1015 1015
Size: 3977
Acc65I G'GTAC_C
Cuts at: 993 993
Size: 3977
AclI AA'CG_TT
Cuts at: 3817 3817
Size: 3977
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1802 2234 2981 3249 1802
Size: 432 747 268 2530
Fragments arranged by size:
2530 747 432 268
AflIII A'CryG_T
Cuts at: 1329 2433 3410 1329
Size: 1104 977 1896
Fragments arranged by size:
1896 1104 977
AgeI A'CCGG_T
Cuts at: 2365 2365
Size: 3977
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 415 447 415
Size: 32 3945
AlwNI CAG_nnn'CTG
Cuts at: 327 678 1282 2849 327
Size: 351 604 1567 1455
Fragments arranged by size:
1567 1455 604 351
ApaLI G'TGCA_C
Cuts at: 2747 2747
Size: 3977
ApoI r'AATT_y
Cuts at: 981 3330 3586 981
Size: 2349 256 1372
Fragments arranged by size:
2349 1372 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3977
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3977
AvaI C'yCGr_G
Cuts at: 997 997
Size: 3977
BamHI G'GATC_C
Cuts at: 548 1002 548
Size: 454 3523
BanI G'GyrC_C
Cuts at: 993 1407 1655 1690 993
Size: 414 248 35 3280
Fragments arranged by size:
3280 414 248 35
BanII G_rGCy'C
Cuts at: 991 2021 991
Size: 1030 2947
BbeI G_GCGC'C
Cuts at: 1411 1659 1411
Size: 248 3729
BbsI GAAGACnn'nnnn_
Cuts at: 319 361 608 319
Size: 42 247 3688
Fragments arranged by size:
3688 247 42
BciVI GTATCCnnnnn_n'
Cuts at: 1875 2636 3977 1875
Size: 761 1341 1875
Fragments arranged by size:
1875 1341 761
BclI T'GATC_A
Cuts at: 905 1497 905
Size: 592 3385
BglI GCCn_nnn'nGGC
Cuts at: 289 289
Size: 3977
BglII A'GATC_T
Cuts at: 1492 3529 1492
Size: 2037 1940
BlpI GC'TnA_GC
Cuts at: 1089 1089
Size: 3977
Bme1580I G_kGCm'C
Cuts at: 1602 1695 2751 1602
Size: 93 1056 2828
Fragments arranged by size:
2828 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 510 1189 1270 1605 3749 510
Size: 679 81 335 2144 738
Fragments arranged by size:
2144 738 679 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2344 2344
Size: 3977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 583 631 739 349
Size: 234 48 108 3587
Fragments arranged by size:
3587 234 108 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 107 1153 2032 2524 2822 107
Size: 1046 879 492 298 1262
Fragments arranged by size:
1262 1046 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 715 3681 715
Size: 2966 1011
BsaAI yAC'GTr
Cuts at: 174 834 1330 174
Size: 660 496 2821
Fragments arranged by size:
2821 660 496
BsaBI GATnn'nnATC
Cuts at: 6 1491 3528 6
Size: 1485 2037 455
Fragments arranged by size:
2037 1485 455
BsaHI Gr'CG_yC
Cuts at: 943 1408 1656 2358 3854 943
Size: 465 248 702 1496 1066
Fragments arranged by size:
1496 1066 702 465 248
BsaWI w'CCGG_w
Cuts at: 545 1166 1687 2365 2639 2786 3197 545
Size: 621 521 678 274 147 411 1325
Fragments arranged by size:
1325 678 621 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 415 445 415
Size: 30 3947
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 427 175
Size: 252 3725
BseYI C'CCAG_C
Cuts at: 458 927 963 1402 2160 2737 3233 458
Size: 469 36 439 758 577 496 1202
Fragments arranged by size:
1202 758 577 496 469 439 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 724
Size: 3977
BsiEI CG_ry'CG
Cuts at: 104 392 1519 1565 2773 104
Size: 288 1127 46 1208 1308
Fragments arranged by size:
1308 1208 1127 288 46
BsiHKAI G_wGCw'C
Cuts at: 991 1769 1959 2751 991
Size: 778 190 792 2217
Fragments arranged by size:
2217 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 685 685
Size: 3977
Bsp1286I G_dGCh'C
Cuts at: 991 1602 1695 1769 1959 2021 2751 991
Size: 611 93 74 190 62 730 2217
Fragments arranged by size:
2217 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3197 3197
Size: 3977
BspHI T'CATG_A
Cuts at: 902 3153 902
Size: 2251 1726
BspMI ACCTGCnnnn'nnnn_
Cuts at: 252 449 1027 1241 1543 1924 252
Size: 197 578 214 302 381 2305
Fragments arranged by size:
2305 578 381 302 214 197
BsrBI CCG'CTC
Cuts at: 50 617 2269 2323 3807 3972 50
Size: 567 1652 54 1484 165 55
Fragments arranged by size:
1652 1484 567 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1889 1889
Size: 3977
BsrFI r'CCGG_y
Cuts at: 1975 2156 2365 1975
Size: 181 209 3587
Fragments arranged by size:
3587 209 181
BssSI C'ACGA_G
Cuts at: 2248 2606 2248
Size: 358 3619
BstAPI GCAn_nnn'nTGC
Cuts at: 1354 1354
Size: 3977
BstBI TT'CG_AA
Cuts at: 2338 2338
Size: 3977
BstYI r'GATC_y
Cuts at: 1 221 333 548 1002 1469 1492 1827 2073
Size: 220 112 215 454 467 23 335 246
Cuts at: 2073 3085 3171 3183 3529 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 454 449 346 335 246 220
215 112 86 23 12
BtgI C'CryG_G
Cuts at: 434 434
Size: 3977
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1927 2108 2271 1927
Size: 181 163 3633
Fragments arranged by size:
3633 181 163
BtsI GCAGTG_nn'
Cuts at: 338 872 1220 338
Size: 534 348 3095
Fragments arranged by size:
3095 534 348
DraI TTT'AAA
Cuts at: 976 976
Size: 3977
DrdI GACnn_nn'nnGTC
Cuts at: 1683 2541 1683
Size: 858 3119
EaeI y'GGCC_r
Cuts at: 290 359 626 761 1562 1736 2127 2154 3499
Size: 69 267 135 801 174 391 27 1345
Cuts at: 3499 290
Size: 768
Fragments arranged by size:
1345 801 768 391 267 174 135 69
27
EagI C'GGCC_G
Cuts at: 1562 1562
Size: 3977
EarI CTCTTCn'nnn_
Cuts at: 152 212 2000 2210 152
Size: 60 1788 210 1919
Fragments arranged by size:
1919 1788 210 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2185 2495 2641 3787 2185
Size: 310 146 1146 2375
Fragments arranged by size:
2375 1146 310 146
EcoICRI GAG'CTC
Cuts at: 989 989
Size: 3977
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 583 631 739 1802 2234 2981 3249 349
Size: 234 48 108 1063 432 747 268 1077
Fragments arranged by size:
1077 1063 747 432 268 234 108 48
EcoO109I rG'GnC_Cy
Cuts at: 1116 1116
Size: 3977
EcoRI G'AATT_C
Cuts at: 981 981
Size: 3977
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1996 1996
Size: 3977
FspI TGC'GCA
Cuts at: 445 1758 445
Size: 1313 2664
HaeII r_GCGC'y
Cuts at: 284 679 1411 1659 2681 3660 284
Size: 395 732 248 1022 979 601
Fragments arranged by size:
1022 979 732 601 395 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 229 261 1819 1849 1851 1881 229
Size: 32 1558 30 2 30 2325
Fragments arranged by size:
2325 1558 32 30 30 2
HincII GTy'rAC
Cuts at: 1016 1016
Size: 3977
KasI G'GCGC_C
Cuts at: 1407 1655 1407
Size: 248 3729
KpnI G_GTAC'C
Cuts at: 997 997
Size: 3977
MluI A'CGCG_T
Cuts at: 3410 3410
Size: 3977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 227 2648 2832 227
Size: 2421 184 1372
Fragments arranged by size:
2421 1372 184
MscI TGG'CCA
Cuts at: 628 763 1738 628
Size: 135 975 2867
Fragments arranged by size:
2867 975 135
MslI CAynn'nnrTG
Cuts at: 649 763 1966 2093 649
Size: 114 1203 127 2533
Fragments arranged by size:
2533 1203 127 114
MspA1I CmG'CkG
Cuts at: 436 536 555 1088 1402 1762 2775 3020 436
Size: 100 19 533 314 360 1013 245 1393
Fragments arranged by size:
1393 1013 533 360 314 245 100 19
NaeI GCC'GGC
Cuts at: 2158 2158
Size: 3977
NarI GG'CG_CC
Cuts at: 1408 1656 1408
Size: 248 3729
NgoMIV G'CCGG_C
Cuts at: 2156 2156
Size: 3977
NspI r_CATG'y
Cuts at: 609 1030 1530 2437 609
Size: 421 500 907 2149
Fragments arranged by size:
2149 907 500 421
PciI A'CATG_T
Cuts at: 2433 2433
Size: 3977
PflMI CCAn_nnn'nTGG
Cuts at: 192 192
Size: 3977
PfoI T'CCnGG_A
Cuts at: 475 475
Size: 3977
PmeI GTTT'AAAC
Cuts at: 976 976
Size: 3977
PmlI CAC'GTG
Cuts at: 174 174
Size: 3977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 415 447 3116 3148 415
Size: 32 2669 32 1244
Fragments arranged by size:
2669 1244 32 32
PstI C_TGCA'G
Cuts at: 345 1024 1709 345
Size: 679 685 2613
Fragments arranged by size:
2613 685 679
PvuI CG_AT'CG
Cuts at: 104 392 104
Size: 288 3689
PvuII CAG'CTG
Cuts at: 1402 1762 1402
Size: 360 3617
RsrII CG'GwC_CG
Cuts at: 2172 2172
Size: 3977
SacI G_AGCT'C
Cuts at: 991 991
Size: 3977
SacII CC_GC'GG
Cuts at: 437 437
Size: 3977
SalI G'TCGA_C
Cuts at: 1014 1014
Size: 3977
SapI GCTCTTCn'nnn_
Cuts at: 2000 2210 2000
Size: 210 3767
SbfI CC_TGCA'GG
Cuts at: 1024 1024
Size: 3977
ScaI AGT'ACT
Cuts at: 3494 3494
Size: 3977
SfcI C'TryA_G
Cuts at: 33 341 1020 1705 2698 2889 33
Size: 308 679 685 993 191 1121
Fragments arranged by size:
1121 993 685 679 308 191
SfoI GGC'GCC
Cuts at: 1409 1657 1409
Size: 248 3729
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3977
SmaI CCC'GGG
Cuts at: 999 999
Size: 3977
SmlI C'TyrA_G
Cuts at: 122 1132 2047 2539 2801 122
Size: 1010 915 492 262 1298
Fragments arranged by size:
1298 1010 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3977
SphI G_CATG'C
Cuts at: 1030 1530 1030
Size: 500 3477
StyI C'CwwG_G
Cuts at: 1111 1465 1111
Size: 354 3623
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 312 362 514 1560 1693 2152 2361 312
Size: 50 152 1046 133 459 209 1928
Fragments arranged by size:
1928 1046 459 209 152 133 50
TatI w'GTAC_w
Cuts at: 806 3492 806
Size: 2686 1291
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3494 3526 3494
Size: 32 3945
Tth111I GACn'n_nGTC
Cuts at: 516 1774 516
Size: 1258 2719
XbaI T'CTAG_A
Cuts at: 1008 1008
Size: 3977
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 324 594 1472 324
Size: 270 878 2829
Fragments arranged by size:
2829 878 270
XmaI C'CCGG_G
Cuts at: 997 997
Size: 3977
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PfoI PmeI PmlI PpiI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PpuMI PshAI PsiI PspOMI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI