Restriction map for
pF1KB9189
(Circular) (Six-base) MAPSORT of: pF1KB9189.seq Check: 5287 from: 1 to: 4400
pF1KB9189 4400 bp
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AccI GT'mk_AC
Cuts at: 211 1438 211
Size: 1227 3173
Acc65I G'GTAC_C
Cuts at: 1416 1416
Size: 4400
AclI AA'CG_TT
Cuts at: 516 4240 516
Size: 3724 676
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 409 1041 1048 2225 2657 3404 3672 352
Size: 57 632 7 1177 432 747 268 1080
Fragments arranged by size:
1177 1080 747 632 432 268 57 7
AflII C'TTAA_G
Cuts at: 1174 1174
Size: 4400
AflIII A'CryG_T
Cuts at: 1752 2856 3833 1752
Size: 1104 977 2319
Fragments arranged by size:
2319 1104 977
AgeI A'CCGG_T
Cuts at: 2788 2788
Size: 4400
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 274 306 274
Size: 32 4368
AlwNI CAG_nnn'CTG
Cuts at: 1705 3272 1705
Size: 1567 2833
ApaI G_GGCC'C
Cuts at: 1350 1350
Size: 4400
ApaLI G'TGCA_C
Cuts at: 3170 3170
Size: 4400
ApoI r'AATT_y
Cuts at: 301 691 1093 1404 3753 4009 301
Size: 390 402 311 2349 256 692
Fragments arranged by size:
2349 692 402 390 311 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4400
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4400
AvaI C'yCGr_G
Cuts at: 1420 1420
Size: 4400
BamHI G'GATC_C
Cuts at: 545 1425 545
Size: 880 3520
BanI G'GyrC_C
Cuts at: 1416 1830 2078 2113 1416
Size: 414 248 35 3703
Fragments arranged by size:
3703 414 248 35
BanII G_rGCy'C
Cuts at: 1350 1414 2444 1350
Size: 64 1030 3306
Fragments arranged by size:
3306 1030 64
BbeI G_GCGC'C
Cuts at: 1834 2082 1834
Size: 248 4152
BbsI GAAGACnn'nnnn_
Cuts at: 1054 1259 1054
Size: 205 4195
BciVI GTATCCnnnnn_n'
Cuts at: 2298 3059 4400 2298
Size: 761 1341 2298
Fragments arranged by size:
2298 1341 761
BclI T'GATC_A
Cuts at: 1920 1920
Size: 4400
BglII A'GATC_T
Cuts at: 1915 3952 1915
Size: 2037 2363
BlpI GC'TnA_GC
Cuts at: 1512 1512
Size: 4400
Bme1580I G_kGCm'C
Cuts at: 1350 2025 2118 3174 1350
Size: 675 93 1056 2576
Fragments arranged by size:
2576 1056 675 93
BmrI ACTGGGnnnn_n'
Cuts at: 471 1612 1693 2028 4172 471
Size: 1141 81 335 2144 699
Fragments arranged by size:
2144 1141 699 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2767 2767
Size: 4400
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 1291 1576 2455 2947 3245 203
Size: 1088 285 879 492 298 1358
Fragments arranged by size:
1358 1088 879 492 298 285
BsaI GGTCTCn'nnnn_
Cuts at: 120 416 4104 120
Size: 296 3688 416
Fragments arranged by size:
3688 416 296
BsaAI yAC'GTr
Cuts at: 1753 1753
Size: 4400
BsaBI GATnn'nnATC
Cuts at: 6 937 1914 3951 6
Size: 931 977 2037 455
Fragments arranged by size:
2037 977 931 455
BsaHI Gr'CG_yC
Cuts at: 1831 2079 2781 4277 1831
Size: 248 702 1496 1954
Fragments arranged by size:
1954 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1589 2110 2788 3062 3209 3620 1589
Size: 521 678 274 147 411 2369
Fragments arranged by size:
2369 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1288 1291 1288
Size: 3 4397
BseYI C'CCAG_C
Cuts at: 315 1334 1349 1825 2583 3160 3656 315
Size: 1019 15 476 758 577 496 1059
Fragments arranged by size:
1059 1019 758 577 496 476 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 907 670
Size: 237 4163
BsiEI CG_ry'CG
Cuts at: 104 1942 1988 3196 104
Size: 1838 46 1208 1308
Fragments arranged by size:
1838 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1414 2192 2382 3174 1414
Size: 778 190 792 2640
Fragments arranged by size:
2640 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1350 1414 2025 2118 2192 2382 2444 3174 1350
Size: 64 611 93 74 190 62 730 2576
Fragments arranged by size:
2576 730 611 190 93 74 64 62
BspEI T'CCGG_A
Cuts at: 3620 3620
Size: 4400
BspHI T'CATG_A
Cuts at: 707 3576 707
Size: 2869 1531
BspMI ACCTGCnnnn'nnnn_
Cuts at: 500 879 1450 1664 1966 2347 500
Size: 379 571 214 302 381 2553
Fragments arranged by size:
2553 571 381 379 302 214
BsrBI CCG'CTC
Cuts at: 50 2692 2746 4230 4395 50
Size: 2642 54 1484 165 55
Fragments arranged by size:
2642 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 384 668 732 906 2312 384
Size: 284 64 174 1406 2472
Fragments arranged by size:
2472 1406 284 174 64
BsrFI r'CCGG_y
Cuts at: 2398 2579 2788 2398
Size: 181 209 4010
Fragments arranged by size:
4010 209 181
BssSI C'ACGA_G
Cuts at: 540 1198 2671 3029 540
Size: 658 1473 358 1911
Fragments arranged by size:
1911 1473 658 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1777 1777
Size: 4400
BstBI TT'CG_AA
Cuts at: 147 2761 147
Size: 2614 1786
BstEII G'GTnAC_C
Cuts at: 606 606
Size: 4400
BstXI CCAn_nnnn'nTGG
Cuts at: 114 279 1342 114
Size: 165 1063 3172
Fragments arranged by size:
3172 1063 165
BstYI r'GATC_y
Cuts at: 1 545 600 1425 1892 1915 2250 2496 3508
Size: 544 55 825 467 23 335 246 1012
Cuts at: 3508 3594 3606 3952 1
Size: 86 12 346 449
Fragments arranged by size:
1012 825 544 467 449 346 335 246
86 55 23 12
BtgI C'CryG_G
Cuts at: 107 113 119 410 107
Size: 6 6 291 4097
Fragments arranged by size:
4097 291 6 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2350 2531 2694 2350
Size: 181 163 4056
Fragments arranged by size:
4056 181 163
BtsI GCAGTG_nn'
Cuts at: 818 1643 818
Size: 825 3575
DraI TTT'AAA
Cuts at: 1399 1399
Size: 4400
DrdI GACnn_nn'nnGTC
Cuts at: 199 2106 2964 199
Size: 1907 858 1635
Fragments arranged by size:
1907 1635 858
EaeI y'GGCC_r
Cuts at: 110 116 165 1985 2159 2550 2577 3922 110
Size: 6 49 1820 174 391 27 1345 588
Fragments arranged by size:
1820 1345 588 391 174 49 27 6
EagI C'GGCC_G
Cuts at: 1985 1985
Size: 4400
EarI CTCTTCn'nnn_
Cuts at: 134 529 824 1010 2423 2633 134
Size: 395 295 186 1413 210 1901
Fragments arranged by size:
1901 1413 395 295 210 186
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2608 2918 3064 4210 2608
Size: 310 146 1146 2798
Fragments arranged by size:
2798 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1412 1412
Size: 4400
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 409 1041 1048 2225 2657 3404 3672 352
Size: 57 632 7 1177 432 747 268 1080
Fragments arranged by size:
1177 1080 747 632 432 268 57 7
EcoNI CCTnn'n_nnAGG
Cuts at: 496 496
Size: 4400
EcoO109I rG'GnC_Cy
Cuts at: 1346 1539 1346
Size: 193 4207
EcoRI G'AATT_C
Cuts at: 1093 1404 1093
Size: 311 4089
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1189 2419 1189
Size: 1230 3170
FspI TGC'GCA
Cuts at: 2181 2181
Size: 4400
HaeII r_GCGC'y
Cuts at: 1834 2082 3104 4083 1834
Size: 248 1022 979 2151
Fragments arranged by size:
2151 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 783 815 2242 2272 2274 2304 783
Size: 32 1427 30 2 30 2879
Fragments arranged by size:
2879 1427 32 30 30 2
HincII GTy'rAC
Cuts at: 204 395 405 1439 204
Size: 191 10 1034 3165
Fragments arranged by size:
3165 1034 191 10
HindIII A'AGCT_T
Cuts at: 1009 1386 1009
Size: 377 4023
HpaI GTT'AAC
Cuts at: 395 395
Size: 4400
KasI G'GCGC_C
Cuts at: 1830 2078 1830
Size: 248 4152
KpnI G_GTAC'C
Cuts at: 1420 1420
Size: 4400
MluI A'CGCG_T
Cuts at: 3833 3833
Size: 4400
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3071 3255 3071
Size: 184 4216
MscI TGG'CCA
Cuts at: 167 2161 167
Size: 1994 2406
MslI CAynn'nnrTG
Cuts at: 112 277 2389 2516 112
Size: 165 2112 127 1996
Fragments arranged by size:
2112 1996 165 127
MspA1I CmG'CkG
Cuts at: 198 1309 1511 1825 2185 3198 3443 198
Size: 1111 202 314 360 1013 245 1155
Fragments arranged by size:
1155 1111 1013 360 314 245 202
NaeI GCC'GGC
Cuts at: 2581 2581
Size: 4400
NarI GG'CG_CC
Cuts at: 1831 2079 1831
Size: 248 4152
NcoI C'CATG_G
Cuts at: 107 107
Size: 4400
NdeI CA'TA_TG
Cuts at: 1074 1074
Size: 4400
NgoMIV G'CCGG_C
Cuts at: 2579 2579
Size: 4400
NspI r_CATG'y
Cuts at: 1453 1953 2860 1453
Size: 500 907 2993
Fragments arranged by size:
2993 907 500
PasI CC'CwG_GG
Cuts at: 875 875
Size: 4400
PciI A'CATG_T
Cuts at: 2856 2856
Size: 4400
PmeI GTTT'AAAC
Cuts at: 1399 1399
Size: 4400
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1009 1041 3539 3571 1009
Size: 32 2498 32 1838
Fragments arranged by size:
2498 1838 32 32
PspOMI G'GGCC_C
Cuts at: 1346 1346
Size: 4400
PstI C_TGCA'G
Cuts at: 1112 1447 2132 1112
Size: 335 685 3380
Fragments arranged by size:
3380 685 335
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4400
PvuII CAG'CTG
Cuts at: 198 1825 2185 198
Size: 1627 360 2413
Fragments arranged by size:
2413 1627 360
RsrII CG'GwC_CG
Cuts at: 2595 2595
Size: 4400
SacI G_AGCT'C
Cuts at: 1414 1414
Size: 4400
SalI G'TCGA_C
Cuts at: 1437 1437
Size: 4400
SapI GCTCTTCn'nnn_
Cuts at: 134 2423 2633 134
Size: 2289 210 1901
Fragments arranged by size:
2289 1901 210
SbfI CC_TGCA'GG
Cuts at: 1447 1447
Size: 4400
ScaI AGT'ACT
Cuts at: 3917 3917
Size: 4400
SexAI A'CCwGG_T
Cuts at: 333 333
Size: 4400
SfcI C'TryA_G
Cuts at: 33 1108 1321 1443 2128 3121 3312 33
Size: 1075 213 122 685 993 191 1121
Fragments arranged by size:
1121 1075 993 685 213 191 122
SfoI GGC'GCC
Cuts at: 1832 2080 1832
Size: 248 4152
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4400
SmaI CCC'GGG
Cuts at: 1422 1422
Size: 4400
SmlI C'TyrA_G
Cuts at: 218 1174 1270 1555 2470 2962 3224 218
Size: 956 96 285 915 492 262 1394
Fragments arranged by size:
1394 956 915 492 285 262 96
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4400
SphI G_CATG'C
Cuts at: 1453 1953 1453
Size: 500 3900
StuI AGG'CCT
Cuts at: 487 487
Size: 4400
StyI C'CwwG_G
Cuts at: 107 1534 1888 107
Size: 1427 354 2619
Fragments arranged by size:
2619 1427 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1983 2116 2575 2784 1983
Size: 133 459 209 3599
Fragments arranged by size:
3599 459 209 133
TatI w'GTAC_w
Cuts at: 3915 3915
Size: 4400
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3917 3949 3917
Size: 32 4368
Tth111I GACn'n_nGTC
Cuts at: 2197 2197
Size: 4400
XbaI T'CTAG_A
Cuts at: 1431 1431
Size: 4400
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 618 1895 618
Size: 1277 3123
XmaI C'CCGG_G
Cuts at: 1420 1420
Size: 4400
XmnI GAAnn'nnTTC
Cuts at: 143 1093 1288 143
Size: 950 195 3255
Fragments arranged by size:
3255 950 195
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PasI PciI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI
Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI MfeI
NheI NotI NruI NsiI PacI PflMI PfoI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI