Restriction map for
pF1KB9185
(Circular) (Six-base) MAPSORT of: pF1KB9185.seq Check: 7164 from: 1 to: 4241
pF1KB9185 4241 bp
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 158 725 158
Size: 567 3674
AccI GT'mk_AC
Cuts at: 1279 1279
Size: 4241
Acc65I G'GTAC_C
Cuts at: 1257 1257
Size: 4241
AclI AA'CG_TT
Cuts at: 4081 4081
Size: 4241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 786 1059 2066 2498 3245 3513 148
Size: 638 273 1007 432 747 268 876
Fragments arranged by size:
1007 876 747 638 432 273 268
AfeI AGC'GCT
Cuts at: 358 439 358
Size: 81 4160
AflIII A'CryG_T
Cuts at: 611 863 1593 2697 3674 611
Size: 252 730 1104 977 1178
Fragments arranged by size:
1178 1104 977 730 252
AgeI A'CCGG_T
Cuts at: 2629 2629
Size: 4241
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 771 771
Size: 4241
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 517 549 1027 1059 517
Size: 32 478 32 3699
Fragments arranged by size:
3699 478 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1546 3113 1546
Size: 1567 2674
ApaLI G'TGCA_C
Cuts at: 207 3011 207
Size: 2804 1437
ApoI r'AATT_y
Cuts at: 1245 3594 3850 1245
Size: 2349 256 1636
Fragments arranged by size:
2349 1636 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4241
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
AvaI C'yCGr_G
Cuts at: 604 1261 604
Size: 657 3584
BamHI G'GATC_C
Cuts at: 833 1266 833
Size: 433 3808
BanI G'GyrC_C
Cuts at: 587 1257 1671 1919 1954 587
Size: 670 414 248 35 2874
Fragments arranged by size:
2874 670 414 248 35
BanII G_rGCy'C
Cuts at: 460 1255 2285 460
Size: 795 1030 2416
Fragments arranged by size:
2416 1030 795
BbeI G_GCGC'C
Cuts at: 591 1675 1923 591
Size: 1084 248 2909
Fragments arranged by size:
2909 1084 248
BbsI GAAGACnn'nnnn_
Cuts at: 136 159 896 136
Size: 23 737 3481
Fragments arranged by size:
3481 737 23
BbvCI CC'TCA_GC
Cuts at: 583 583
Size: 4241
BciVI GTATCCnnnnn_n'
Cuts at: 2139 2900 4241 2139
Size: 761 1341 2139
Fragments arranged by size:
2139 1341 761
BclI T'GATC_A
Cuts at: 1761 1761
Size: 4241
BglII A'GATC_T
Cuts at: 1756 3793 1756
Size: 2037 2204
BlpI GC'TnA_GC
Cuts at: 1353 1353
Size: 4241
Bme1580I G_kGCm'C
Cuts at: 211 497 1866 1959 3015 211
Size: 286 1369 93 1056 1437
Fragments arranged by size:
1437 1369 1056 286 93
BmgBI CAC'GTC
Cuts at: 512 512
Size: 4241
BmrI ACTGGGnnnn_n'
Cuts at: 129 1453 1534 1869 4013 129
Size: 1324 81 335 2144 357
Fragments arranged by size:
2144 1324 357 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 899 2608 899
Size: 1709 2532
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 300 322 544 943 1185 300
Size: 22 222 399 242 3356
Fragments arranged by size:
3356 399 242 222 22
Bpu10I CC'TnA_GC
Cuts at: 583 689 583
Size: 106 4135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 1417 2296 2788 3086 691
Size: 726 879 492 298 1846
Fragments arranged by size:
1846 879 726 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 646 3945 646
Size: 3299 942
BsaAI yAC'GTr
Cuts at: 492 1594 492
Size: 1102 3139
BsaBI GATnn'nnATC
Cuts at: 6 1755 3792 6
Size: 1749 2037 455
Fragments arranged by size:
2037 1749 455
BsaHI Gr'CG_yC
Cuts at: 588 1091 1672 1920 2622 4118 588
Size: 503 581 248 702 1496 711
Fragments arranged by size:
1496 711 702 581 503 248
BsaWI w'CCGG_w
Cuts at: 1430 1951 2629 2903 3050 3461 1430
Size: 521 678 274 147 411 2210
Fragments arranged by size:
2210 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 295 325 517 547 295
Size: 30 192 30 3989
Fragments arranged by size:
3989 192 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 568 888 568
Size: 320 3921
BseYI C'CCAG_C
Cuts at: 450 828 1666 2424 3001 3497 450
Size: 378 838 758 577 496 1194
Fragments arranged by size:
1194 838 758 577 496 378
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 300 130
Size: 170 4071
BsiEI CG_ry'CG
Cuts at: 104 1783 1829 3037 104
Size: 1679 46 1208 1308
Fragments arranged by size:
1679 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 211 374 460 1255 2033 2223 3015 211
Size: 163 86 795 778 190 792 1437
Fragments arranged by size:
1437 795 792 778 190 163 86
Bsp1286I G_dGCh'C
Cuts at: 211 374 460 497 1255 1866 1959 2033 2223
Size: 163 86 37 758 611 93 74 190
Cuts at: 2223 2285 3015 211
Size: 62 730 1437
Fragments arranged by size:
1437 758 730 611 190 163 93 86
74 62 37
BspEI T'CCGG_A
Cuts at: 3461 3461
Size: 4241
BspHI T'CATG_A
Cuts at: 3417 3417
Size: 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 158 725 1291 1505 1807 2188 158
Size: 567 566 214 302 381 2211
Fragments arranged by size:
2211 567 566 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2533 2587 4071 4236 50
Size: 2483 54 1484 165 55
Fragments arranged by size:
2483 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2153 2153
Size: 4241
BsrFI r'CCGG_y
Cuts at: 225 2239 2420 2629 225
Size: 2014 181 209 1837
Fragments arranged by size:
2014 1837 209 181
BsrGI T'GTAC_A
Cuts at: 325 325
Size: 4241
BssSI C'ACGA_G
Cuts at: 2512 2870 2512
Size: 358 3883
BstAPI GCAn_nnn'nTGC
Cuts at: 1618 1618
Size: 4241
BstBI TT'CG_AA
Cuts at: 2602 2602
Size: 4241
BstYI r'GATC_y
Cuts at: 1 833 1266 1733 1756 2091 2337 3349 3435
Size: 832 433 467 23 335 246 1012 86
Cuts at: 3435 3447 3793 1
Size: 12 346 449
Fragments arranged by size:
1012 832 467 449 433 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 347 569 724 1021 107
Size: 240 222 155 297 3327
Fragments arranged by size:
3327 297 240 222 155
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2191 2372 2535 2191
Size: 181 163 3897
Fragments arranged by size:
3897 181 163
BtsI GCAGTG_nn'
Cuts at: 1484 1484
Size: 4241
DraI TTT'AAA
Cuts at: 1240 1240
Size: 4241
DraIII CAC_nnn'GTG
Cuts at: 323 323
Size: 4241
DrdI GACnn_nn'nnGTC
Cuts at: 571 1947 2805 571
Size: 1376 858 2007
Fragments arranged by size:
2007 1376 858
EaeI y'GGCC_r
Cuts at: 223 1826 2000 2391 2418 3763 223
Size: 1603 174 391 27 1345 701
Fragments arranged by size:
1603 1345 701 391 174 27
EagI C'GGCC_G
Cuts at: 1826 1826
Size: 4241
EarI CTCTTCn'nnn_
Cuts at: 1078 2264 2474 1078
Size: 1186 210 2845
Fragments arranged by size:
2845 1186 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 874 2449 2759 2905 4051 874
Size: 1575 310 146 1146 1064
Fragments arranged by size:
1575 1146 1064 310 146
EcoICRI GAG'CTC
Cuts at: 458 1253 458
Size: 795 3446
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 300 322 544 786 943 1059 1185 2066
Size: 152 22 222 242 157 116 126 881
Cuts at: 2066 2498 3245 3513 148
Size: 432 747 268 876
Fragments arranged by size:
881 876 747 432 268 242 222 157
152 126 116 22
EcoO109I rG'GnC_Cy
Cuts at: 465 1380 465
Size: 915 3326
EcoRI G'AATT_C
Cuts at: 1245 1245
Size: 4241
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 4241
FspI TGC'GCA
Cuts at: 2022 2022
Size: 4241
HaeII r_GCGC'y
Cuts at: 220 360 441 591 1675 1923 2945 3924 220
Size: 140 81 150 1084 248 1022 979 537
Fragments arranged by size:
1084 1022 979 537 248 150 140 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 295 327 957 989 1097 1129 2083 2113 2115
Size: 32 630 32 108 32 954 30 2
Cuts at: 2115 2145 295
Size: 30 2391
Fragments arranged by size:
2391 954 630 108 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1280 1280
Size: 4241
KasI G'GCGC_C
Cuts at: 587 1671 1919 587
Size: 1084 248 2909
Fragments arranged by size:
2909 1084 248
KpnI G_GTAC'C
Cuts at: 1261 1261
Size: 4241
MluI A'CGCG_T
Cuts at: 3674 3674
Size: 4241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 575 2912 3096 575
Size: 2337 184 1720
Fragments arranged by size:
2337 1720 184
MscI TGG'CCA
Cuts at: 2002 2002
Size: 4241
MslI CAynn'nnrTG
Cuts at: 676 868 2230 2357 676
Size: 192 1362 127 2560
Fragments arranged by size:
2560 1362 192 127
MspA1I CmG'CkG
Cuts at: 204 726 778 900 1103 1352 1666 2026 3039
Size: 522 52 122 203 249 314 360 1013
Cuts at: 3039 3284 204
Size: 245 1161
Fragments arranged by size:
1161 1013 522 360 314 249 245 203
122 52
NaeI GCC'GGC
Cuts at: 2422 2422
Size: 4241
NarI GG'CG_CC
Cuts at: 588 1672 1920 588
Size: 1084 248 2909
Fragments arranged by size:
2909 1084 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4241
NgoMIV G'CCGG_C
Cuts at: 2420 2420
Size: 4241
NspI r_CATG'y
Cuts at: 615 867 987 1294 1794 2701 615
Size: 252 120 307 500 907 2155
Fragments arranged by size:
2155 907 500 307 252 120
PciI A'CATG_T
Cuts at: 611 863 2697 611
Size: 252 1834 2155
Fragments arranged by size:
2155 1834 252
PfoI T'CCnGG_A
Cuts at: 959 959
Size: 4241
PmeI GTTT'AAAC
Cuts at: 1240 1240
Size: 4241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 517 549 3380 3412 517
Size: 32 2831 32 1346
Fragments arranged by size:
2831 1346 32 32
PpuMI rG'GwC_Cy
Cuts at: 465 465
Size: 4241
PstI C_TGCA'G
Cuts at: 1288 1973 1288
Size: 685 3556
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4241
PvuII CAG'CTG
Cuts at: 204 778 1666 2026 204
Size: 574 888 360 2419
Fragments arranged by size:
2419 888 574 360
RsrII CG'GwC_CG
Cuts at: 2436 2436
Size: 4241
SacI G_AGCT'C
Cuts at: 460 1255 460
Size: 795 3446
SacII CC_GC'GG
Cuts at: 727 727
Size: 4241
SalI G'TCGA_C
Cuts at: 1278 1278
Size: 4241
SapI GCTCTTCn'nnn_
Cuts at: 2264 2474 2264
Size: 210 4031
SbfI CC_TGCA'GG
Cuts at: 1288 1288
Size: 4241
ScaI AGT'ACT
Cuts at: 3758 3758
Size: 4241
SfcI C'TryA_G
Cuts at: 33 1284 1969 2962 3153 33
Size: 1251 685 993 191 1121
Fragments arranged by size:
1251 1121 993 685 191
SfoI GGC'GCC
Cuts at: 589 1673 1921 589
Size: 1084 248 2909
Fragments arranged by size:
2909 1084 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
SmaI CCC'GGG
Cuts at: 1263 1263
Size: 4241
SmlI C'TyrA_G
Cuts at: 706 1396 2311 2803 3065 706
Size: 690 915 492 262 1882
Fragments arranged by size:
1882 915 690 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4241
SphI G_CATG'C
Cuts at: 1294 1794 1294
Size: 500 3741
StyI C'CwwG_G
Cuts at: 107 461 731 1375 1729 107
Size: 354 270 644 354 2619
Fragments arranged by size:
2619 644 354 354 270
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 410 1824 1957 2416 2625 410
Size: 1414 133 459 209 2026
Fragments arranged by size:
2026 1414 459 209 133
TatI w'GTAC_w
Cuts at: 325 608 626 3756 325
Size: 283 18 3130 810
Fragments arranged by size:
3130 810 283 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 417 449 3758 3790 417
Size: 32 3309 32 868
Fragments arranged by size:
3309 868 32 32
Tth111I GACn'n_nGTC
Cuts at: 2038 2038
Size: 4241
XbaI T'CTAG_A
Cuts at: 1272 1272
Size: 4241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1736 114
Size: 1622 2619
XmaI C'CCGG_G
Cuts at: 1261 1261
Size: 4241
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI ApaI AscI AvrII BaeI
BcgI BfrBI BglI BmtI BsiWI BsmI BsmBI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PsiI
PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI