Restriction map for
pF1KB9140
(Circular) (Six-base) MAPSORT of: pF1KB9140.seq Check: 2106 from: 1 to: 4241
pF1KB9140 4241 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AccI GT'mk_AC
Cuts at: 1279 1279
Size: 4241
Acc65I G'GTAC_C
Cuts at: 1257 1257
Size: 4241
AclI AA'CG_TT
Cuts at: 4081 4081
Size: 4241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2066 2498 3245 3513 2066
Size: 432 747 268 2794
Fragments arranged by size:
2794 747 432 268
AflII C'TTAA_G
Cuts at: 369 369
Size: 4241
AflIII A'CryG_T
Cuts at: 1593 2697 3674 1593
Size: 1104 977 2160
Fragments arranged by size:
2160 1104 977
AgeI A'CCGG_T
Cuts at: 2629 2629
Size: 4241
AlwNI CAG_nnn'CTG
Cuts at: 1066 1546 3113 1066
Size: 480 1567 2194
Fragments arranged by size:
2194 1567 480
ApaLI G'TGCA_C
Cuts at: 3011 3011
Size: 4241
ApoI r'AATT_y
Cuts at: 715 1245 3594 3850 715
Size: 530 2349 256 1106
Fragments arranged by size:
2349 1106 530 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4241
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
AvaI C'yCGr_G
Cuts at: 236 1261 236
Size: 1025 3216
BamHI G'GATC_C
Cuts at: 1266 1266
Size: 4241
BanI G'GyrC_C
Cuts at: 125 1257 1671 1919 1954 125
Size: 1132 414 248 35 2412
Fragments arranged by size:
2412 1132 414 248 35
BanII G_rGCy'C
Cuts at: 144 226 869 1255 2285 144
Size: 82 643 386 1030 2100
Fragments arranged by size:
2100 1030 643 386 82
BbeI G_GCGC'C
Cuts at: 129 1675 1923 129
Size: 1546 248 2447
Fragments arranged by size:
2447 1546 248
BbsI GAAGACnn'nnnn_
Cuts at: 942 1154 942
Size: 212 4029
BciVI GTATCCnnnnn_n'
Cuts at: 2139 2900 4241 2139
Size: 761 1341 2139
Fragments arranged by size:
2139 1341 761
BclI T'GATC_A
Cuts at: 1761 1761
Size: 4241
BglII A'GATC_T
Cuts at: 615 1756 3793 615
Size: 1141 2037 1063
Fragments arranged by size:
2037 1141 1063
BlpI GC'TnA_GC
Cuts at: 1353 1353
Size: 4241
Bme1580I G_kGCm'C
Cuts at: 1016 1866 1959 3015 1016
Size: 850 93 1056 2242
Fragments arranged by size:
2242 1056 850 93
BmrI ACTGGGnnnn_n'
Cuts at: 1453 1534 1869 4013 1453
Size: 81 335 2144 1681
Fragments arranged by size:
2144 1681 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2608 2608
Size: 4241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 128 1025 1205 128
Size: 897 180 3164
Fragments arranged by size:
3164 897 180
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1417 2296 2788 3086 1417
Size: 879 492 298 2572
Fragments arranged by size:
2572 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 254 3945 254
Size: 3691 550
BsaAI yAC'GTr
Cuts at: 1594 1594
Size: 4241
BsaBI GATnn'nnATC
Cuts at: 6 1755 3792 6
Size: 1749 2037 455
Fragments arranged by size:
2037 1749 455
BsaHI Gr'CG_yC
Cuts at: 126 1672 1920 2622 4118 126
Size: 1546 248 702 1496 249
Fragments arranged by size:
1546 1496 702 249 248
BsaWI w'CCGG_w
Cuts at: 1430 1951 2629 2903 3050 3461 1430
Size: 521 678 274 147 411 2210
Fragments arranged by size:
2210 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 199 603 199
Size: 404 3837
BseYI C'CCAG_C
Cuts at: 653 1666 2424 3001 3497 653
Size: 1013 758 577 496 1397
Fragments arranged by size:
1397 1013 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 137 1783 1829 3037 104
Size: 33 1646 46 1208 1308
Fragments arranged by size:
1646 1308 1208 46 33
BsiHKAI G_wGCw'C
Cuts at: 144 226 869 1255 2033 2223 3015 144
Size: 82 643 386 778 190 792 1370
Fragments arranged by size:
1370 792 778 643 386 190 82
Bsp1286I G_dGCh'C
Cuts at: 144 226 869 1016 1255 1866 1959 2033 2223
Size: 82 643 147 239 611 93 74 190
Cuts at: 2223 2285 3015 144
Size: 62 730 1370
Fragments arranged by size:
1370 730 643 611 239 190 147 93
82 74 62
BspEI T'CCGG_A
Cuts at: 3461 3461
Size: 4241
BspHI T'CATG_A
Cuts at: 195 869 3417 195
Size: 674 2548 1019
Fragments arranged by size:
2548 1019 674
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1291 1505 1807 2188 1291
Size: 214 302 381 3344
Fragments arranged by size:
3344 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2533 2587 4071 4236 50
Size: 2483 54 1484 165 55
Fragments arranged by size:
2483 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2153 2153
Size: 4241
BsrFI r'CCGG_y
Cuts at: 2239 2420 2629 2239
Size: 181 209 3851
Fragments arranged by size:
3851 209 181
BssSI C'ACGA_G
Cuts at: 2512 2870 2512
Size: 358 3883
BstAPI GCAn_nnn'nTGC
Cuts at: 1618 1618
Size: 4241
BstBI TT'CG_AA
Cuts at: 2602 2602
Size: 4241
BstYI r'GATC_y
Cuts at: 1 615 1032 1062 1266 1733 1756 2091 2337
Size: 614 417 30 204 467 23 335 246
Cuts at: 2337 3349 3435 3447 3793 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 614 467 449 417 346 335 246
204 86 30 23 12
Bsu36I CC'TnA_GG
Cuts at: 662 662
Size: 4241
BtgI C'CryG_G
Cuts at: 179 349 179
Size: 170 4071
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2191 2372 2535 2191
Size: 181 163 3897
Fragments arranged by size:
3897 181 163
BtsI GCAGTG_nn'
Cuts at: 363 1484 363
Size: 1121 3120
DraI TTT'AAA
Cuts at: 1240 1240
Size: 4241
DrdI GACnn_nn'nnGTC
Cuts at: 1947 2805 1947
Size: 858 3383
EaeI y'GGCC_r
Cuts at: 1826 2000 2391 2418 3763 1826
Size: 174 391 27 1345 2304
Fragments arranged by size:
2304 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1826 1826
Size: 4241
EarI CTCTTCn'nnn_
Cuts at: 212 839 1116 2264 2474 212
Size: 627 277 1148 210 1979
Fragments arranged by size:
1979 1148 627 277 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2449 2759 2905 4051 2449
Size: 310 146 1146 2639
Fragments arranged by size:
2639 1146 310 146
EcoICRI GAG'CTC
Cuts at: 142 224 867 1253 142
Size: 82 643 386 3130
Fragments arranged by size:
3130 643 386 82
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 128 1025 1205 2066 2498 3245 3513 128
Size: 897 180 861 432 747 268 856
Fragments arranged by size:
897 861 856 747 432 268 180
EcoO109I rG'GnC_Cy
Cuts at: 1380 1380
Size: 4241
EcoRI G'AATT_C
Cuts at: 1245 1245
Size: 4241
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 296 2260 296
Size: 1964 2277
FspI TGC'GCA
Cuts at: 583 2022 583
Size: 1439 2802
HaeII r_GCGC'y
Cuts at: 129 1675 1923 2945 3924 129
Size: 1546 248 1022 979 446
Fragments arranged by size:
1546 1022 979 446 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 126 158 190 222 758 790 2083 2113 2115
Size: 32 32 32 536 32 1293 30 2
Cuts at: 2115 2145 126
Size: 30 2222
Fragments arranged by size:
2222 1293 536 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1280 1280
Size: 4241
KasI G'GCGC_C
Cuts at: 125 1671 1919 125
Size: 1546 248 2447
Fragments arranged by size:
2447 1546 248
KpnI G_GTAC'C
Cuts at: 1261 1261
Size: 4241
MluI A'CGCG_T
Cuts at: 3674 3674
Size: 4241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1219 2912 3096 1219
Size: 1693 184 2364
Fragments arranged by size:
2364 1693 184
MscI TGG'CCA
Cuts at: 2002 2002
Size: 4241
MslI CAynn'nnrTG
Cuts at: 2230 2357 2230
Size: 127 4114
MspA1I CmG'CkG
Cuts at: 161 1352 1666 2026 3039 3284 161
Size: 1191 314 360 1013 245 1118
Fragments arranged by size:
1191 1118 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2422 2422
Size: 4241
NarI GG'CG_CC
Cuts at: 126 1672 1920 126
Size: 1546 248 2447
Fragments arranged by size:
2447 1546 248
NcoI C'CATG_G
Cuts at: 179 179
Size: 4241
NgoMIV G'CCGG_C
Cuts at: 2420 2420
Size: 4241
NspI r_CATG'y
Cuts at: 995 1294 1794 2701 995
Size: 299 500 907 2535
Fragments arranged by size:
2535 907 500 299
PciI A'CATG_T
Cuts at: 2697 2697
Size: 4241
PmeI GTTT'AAAC
Cuts at: 1240 1240
Size: 4241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3380 3412 3380
Size: 32 4209
PspXI vC'TCGA_Gb
Cuts at: 236 236
Size: 4241
PstI C_TGCA'G
Cuts at: 1288 1973 1288
Size: 685 3556
PvuI CG_AT'CG
Cuts at: 104 137 104
Size: 33 4208
PvuII CAG'CTG
Cuts at: 161 1666 2026 161
Size: 1505 360 2376
Fragments arranged by size:
2376 1505 360
RsrII CG'GwC_CG
Cuts at: 2436 2436
Size: 4241
SacI G_AGCT'C
Cuts at: 144 226 869 1255 144
Size: 82 643 386 3130
Fragments arranged by size:
3130 643 386 82
SalI G'TCGA_C
Cuts at: 1278 1278
Size: 4241
SapI GCTCTTCn'nnn_
Cuts at: 839 2264 2474 839
Size: 1425 210 2606
Fragments arranged by size:
2606 1425 210
SbfI CC_TGCA'GG
Cuts at: 1288 1288
Size: 4241
ScaI AGT'ACT
Cuts at: 3758 3758
Size: 4241
SfcI C'TryA_G
Cuts at: 33 414 1284 1969 2962 3153 33
Size: 381 870 685 993 191 1121
Fragments arranged by size:
1121 993 870 685 381 191
SfoI GGC'GCC
Cuts at: 127 1673 1921 127
Size: 1546 248 2447
Fragments arranged by size:
2447 1546 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
SmaI CCC'GGG
Cuts at: 1263 1263
Size: 4241
SmlI C'TyrA_G
Cuts at: 236 369 1396 2311 2803 3065 236
Size: 133 1027 915 492 262 1412
Fragments arranged by size:
1412 1027 915 492 262 133
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4241
SphI G_CATG'C
Cuts at: 1294 1794 1294
Size: 500 3741
SspI AAT'ATT
Cuts at: 1173 1173
Size: 4241
StyI C'CwwG_G
Cuts at: 179 1375 1729 179
Size: 1196 354 2691
Fragments arranged by size:
2691 1196 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1127 1824 1957 2416 2625 1127
Size: 697 133 459 209 2743
Fragments arranged by size:
2743 697 459 209 133
TatI w'GTAC_w
Cuts at: 3756 3756
Size: 4241
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3758 3790 3758
Size: 32 4209
Tth111I GACn'n_nGTC
Cuts at: 202 1142 2038 202
Size: 940 896 2405
Fragments arranged by size:
2405 940 896
XbaI T'CTAG_A
Cuts at: 1272 1272
Size: 4241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 186 1736 186
Size: 1550 2691
XhoI C'TCGA_G
Cuts at: 236 236
Size: 4241
XmaI C'CCGG_G
Cuts at: 1261 1261
Size: 4241
XmnI GAAnn'nnTTC
Cuts at: 1161 1161
Size: 4241
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI ZraI