Restriction map for
pF1KB8991
(Circular) (Six-base) MAPSORT of: pF1KB8991.seq Check: 5717 from: 1 to: 3578
pF1KB8991 3578 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 616 616
Size: 3578
Acc65I G'GTAC_C
Cuts at: 594 594
Size: 3578
AclI AA'CG_TT
Cuts at: 3418 3418
Size: 3578
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1403 1835 2582 2850 1403
Size: 432 747 268 2131
Fragments arranged by size:
2131 747 432 268
AflIII A'CryG_T
Cuts at: 930 2034 3011 930
Size: 1104 977 1497
Fragments arranged by size:
1497 1104 977
AgeI A'CCGG_T
Cuts at: 1966 1966
Size: 3578
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 172 204 172
Size: 32 3546
AlwNI CAG_nnn'CTG
Cuts at: 883 2450 883
Size: 1567 2011
ApaI G_GGCC'C
Cuts at: 115 115
Size: 3578
ApaLI G'TGCA_C
Cuts at: 2348 2348
Size: 3578
ApoI r'AATT_y
Cuts at: 143 582 2931 3187 143
Size: 439 2349 256 534
Fragments arranged by size:
2349 534 439 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3578
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3578
AvaI C'yCGr_G
Cuts at: 115 333 598 115
Size: 218 265 3095
Fragments arranged by size:
3095 265 218
BamHI G'GATC_C
Cuts at: 603 603
Size: 3578
BanI G'GyrC_C
Cuts at: 594 1008 1256 1291 594
Size: 414 248 35 2881
Fragments arranged by size:
2881 414 248 35
BanII G_rGCy'C
Cuts at: 115 158 592 1622 115
Size: 43 434 1030 2071
Fragments arranged by size:
2071 1030 434 43
BbeI G_GCGC'C
Cuts at: 1012 1260 1012
Size: 248 3330
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 324 358 324
Size: 34 3544
BciVI GTATCCnnnnn_n'
Cuts at: 1476 2237 3578 1476
Size: 761 1341 1476
Fragments arranged by size:
1476 1341 761
BclI T'GATC_A
Cuts at: 1098 1098
Size: 3578
BfrBI ATG'CAT
Cuts at: 441 441
Size: 3578
BglI GCCn_nnn'nGGC
Cuts at: 409 409
Size: 3578
BglII A'GATC_T
Cuts at: 1093 3130 1093
Size: 2037 1541
BlpI GC'TnA_GC
Cuts at: 690 690
Size: 3578
Bme1580I G_kGCm'C
Cuts at: 115 1203 1296 2352 115
Size: 1088 93 1056 1341
Fragments arranged by size:
1341 1088 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 790 871 1206 3350 790
Size: 81 335 2144 1018
Fragments arranged by size:
2144 1018 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1945 1945
Size: 3578
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 295 142
Size: 153 3425
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 1633 2125 2423 754
Size: 879 492 298 1909
Fragments arranged by size:
1909 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3282 3282
Size: 3578
BsaAI yAC'GTr
Cuts at: 931 931
Size: 3578
BsaBI GATnn'nnATC
Cuts at: 6 1092 3129 6
Size: 1086 2037 455
Fragments arranged by size:
2037 1086 455
BsaHI Gr'CG_yC
Cuts at: 1009 1257 1959 3455 1009
Size: 248 702 1496 1132
Fragments arranged by size:
1496 1132 702 248
BsaWI w'CCGG_w
Cuts at: 567 767 1288 1966 2240 2387 2798 567
Size: 200 521 678 274 147 411 1347
Fragments arranged by size:
1347 678 521 411 274 200 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 172 202 172
Size: 30 3548
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 262 391 262
Size: 129 3449
BseYI C'CCAG_C
Cuts at: 1003 1761 2338 2834 1003
Size: 758 577 496 1747
Fragments arranged by size:
1747 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 364 514 340
Size: 24 150 3404
Fragments arranged by size:
3404 150 24
BsiEI CG_ry'CG
Cuts at: 104 1120 1166 2374 104
Size: 1016 46 1208 1308
Fragments arranged by size:
1308 1208 1016 46
BsiHKAI G_wGCw'C
Cuts at: 235 592 1370 1560 2352 235
Size: 357 778 190 792 1461
Fragments arranged by size:
1461 792 778 357 190
Bsp1286I G_dGCh'C
Cuts at: 115 158 235 592 1203 1296 1370 1560 1622
Size: 43 77 357 611 93 74 190 62
Cuts at: 1622 2352 115
Size: 730 1341
Fragments arranged by size:
1341 730 611 357 190 93 77 74
62 43
BspEI T'CCGG_A
Cuts at: 567 2798 567
Size: 2231 1347
BspHI T'CATG_A
Cuts at: 2754 2754
Size: 3578
BspMI ACCTGCnnnn'nnnn_
Cuts at: 628 842 1144 1525 628
Size: 214 302 381 2681
Fragments arranged by size:
2681 381 302 214
BsrBI CCG'CTC
Cuts at: 50 178 1870 1924 3408 3573 50
Size: 128 1692 54 1484 165 55
Fragments arranged by size:
1692 1484 165 128 55 54
BsrDI GCAATG_nn'
Cuts at: 1490 1490
Size: 3578
BsrFI r'CCGG_y
Cuts at: 1576 1757 1966 1576
Size: 181 209 3188
Fragments arranged by size:
3188 209 181
BssHII G'CGCG_C
Cuts at: 300 324 300
Size: 24 3554
BssSI C'ACGA_G
Cuts at: 1849 2207 1849
Size: 358 3220
BstAPI GCAn_nnn'nTGC
Cuts at: 955 955
Size: 3578
BstBI TT'CG_AA
Cuts at: 1939 1939
Size: 3578
BstYI r'GATC_y
Cuts at: 1 603 1070 1093 1428 1674 2686 2772 2784
Size: 602 467 23 335 246 1012 86 12
Cuts at: 2784 3130 1
Size: 346 449
Fragments arranged by size:
1012 602 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3578
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1528 1709 1872 1528
Size: 181 163 3234
Fragments arranged by size:
3234 181 163
BtsI GCAGTG_nn'
Cuts at: 821 821
Size: 3578
DraI TTT'AAA
Cuts at: 577 577
Size: 3578
DrdI GACnn_nn'nnGTC
Cuts at: 1284 2142 1284
Size: 858 2720
EaeI y'GGCC_r
Cuts at: 410 1163 1337 1728 1755 3100 410
Size: 753 174 391 27 1345 888
Fragments arranged by size:
1345 888 753 391 174 27
EagI C'GGCC_G
Cuts at: 1163 1163
Size: 3578
EarI CTCTTCn'nnn_
Cuts at: 1601 1811 1601
Size: 210 3368
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 115 439 1786 2096 2242 3388 115
Size: 324 1347 310 146 1146 305
Fragments arranged by size:
1347 1146 324 310 305 146
EcoICRI GAG'CTC
Cuts at: 590 590
Size: 3578
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 295 1403 1835 2582 2850 142
Size: 153 1108 432 747 268 870
Fragments arranged by size:
1108 870 747 432 268 153
EcoO109I rG'GnC_Cy
Cuts at: 112 252 279 717 112
Size: 140 27 438 2973
Fragments arranged by size:
2973 438 140 27
EcoRI G'AATT_C
Cuts at: 582 582
Size: 3578
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1597 1597
Size: 3578
FspI TGC'GCA
Cuts at: 1359 1359
Size: 3578
HaeII r_GCGC'y
Cuts at: 1012 1260 2282 3261 1012
Size: 248 1022 979 1329
Fragments arranged by size:
1329 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1420 1450 1452 1482 1420
Size: 30 2 30 3516
Fragments arranged by size:
3516 30 30 2
HincII GTy'rAC
Cuts at: 617 617
Size: 3578
KasI G'GCGC_C
Cuts at: 1008 1256 1008
Size: 248 3330
KpnI G_GTAC'C
Cuts at: 598 598
Size: 3578
MfeI C'AATT_G
Cuts at: 499 499
Size: 3578
MluI A'CGCG_T
Cuts at: 3011 3011
Size: 3578
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2249 2433 2249
Size: 184 3394
MscI TGG'CCA
Cuts at: 412 1339 412
Size: 927 2651
MslI CAynn'nnrTG
Cuts at: 490 1567 1694 490
Size: 1077 127 2374
Fragments arranged by size:
2374 1077 127
MspA1I CmG'CkG
Cuts at: 131 270 459 689 1003 1363 2376 2621 131
Size: 139 189 230 314 360 1013 245 1088
Fragments arranged by size:
1088 1013 360 314 245 230 189 139
NaeI GCC'GGC
Cuts at: 1759 1759
Size: 3578
NarI GG'CG_CC
Cuts at: 1009 1257 1009
Size: 248 3330
NcoI C'CATG_G
Cuts at: 107 107
Size: 3578
NgoMIV G'CCGG_C
Cuts at: 1757 1757
Size: 3578
NsiI A_TGCA'T
Cuts at: 443 443
Size: 3578
NspI r_CATG'y
Cuts at: 631 1131 2038 631
Size: 500 907 2171
Fragments arranged by size:
2171 907 500
PasI CC'CwG_GG
Cuts at: 150 150
Size: 3578
PciI A'CATG_T
Cuts at: 2034 2034
Size: 3578
PflMI CCAn_nnn'nTGG
Cuts at: 459 459
Size: 3578
PfoI T'CCnGG_A
Cuts at: 242 242
Size: 3578
PmeI GTTT'AAAC
Cuts at: 577 577
Size: 3578
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 172 204 2717 2749 172
Size: 32 2513 32 1001
Fragments arranged by size:
2513 1001 32 32
PpuMI rG'GwC_Cy
Cuts at: 279 279
Size: 3578
PspOMI G'GGCC_C
Cuts at: 111 111
Size: 3578
PstI C_TGCA'G
Cuts at: 136 625 1310 136
Size: 489 685 2404
Fragments arranged by size:
2404 685 489
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3578
PvuII CAG'CTG
Cuts at: 459 1003 1363 459
Size: 544 360 2674
Fragments arranged by size:
2674 544 360
RsrII CG'GwC_CG
Cuts at: 1773 1773
Size: 3578
SacI G_AGCT'C
Cuts at: 592 592
Size: 3578
SalI G'TCGA_C
Cuts at: 615 615
Size: 3578
SapI GCTCTTCn'nnn_
Cuts at: 1601 1811 1601
Size: 210 3368
SbfI CC_TGCA'GG
Cuts at: 625 625
Size: 3578
ScaI AGT'ACT
Cuts at: 3095 3095
Size: 3578
SfcI C'TryA_G
Cuts at: 33 132 621 1306 2299 2490 33
Size: 99 489 685 993 191 1121
Fragments arranged by size:
1121 993 685 489 191 99
SfiI GGCCn_nnn'nGGCC
Cuts at: 409 409
Size: 3578
SfoI GGC'GCC
Cuts at: 1010 1258 1010
Size: 248 3330
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3578
SmaI CCC'GGG
Cuts at: 117 600 117
Size: 483 3095
SmlI C'TyrA_G
Cuts at: 333 733 1648 2140 2402 333
Size: 400 915 492 262 1509
Fragments arranged by size:
1509 915 492 400 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3578
SphI G_CATG'C
Cuts at: 631 1131 631
Size: 500 3078
StyI C'CwwG_G
Cuts at: 107 712 1066 107
Size: 605 354 2619
Fragments arranged by size:
2619 605 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1161 1294 1753 1962 1161
Size: 133 459 209 2777
Fragments arranged by size:
2777 459 209 133
TatI w'GTAC_w
Cuts at: 3093 3093
Size: 3578
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3095 3127 3095
Size: 32 3546
Tth111I GACn'n_nGTC
Cuts at: 1375 1375
Size: 3578
XbaI T'CTAG_A
Cuts at: 609 609
Size: 3578
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 270 1073 270
Size: 803 2775
XhoI C'TCGA_G
Cuts at: 333 333
Size: 3578
XmaI C'CCGG_G
Cuts at: 115 598 115
Size: 483 3095
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbsI BbvCI BmgBI BmtI Bpu10I BsiWI
BsmI BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NruI PacI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI
SspI StuI SwaI XmnI ZraI