Restriction map for
pF1KB8977
(Circular) (Six-base) MAPSORT of: pF1KB8977.seq Check: 9110 from: 1 to: 4232
pF1KB8977 4232 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 1270 1270
Size: 4232
Acc65I G'GTAC_C
Cuts at: 875 1248 875
Size: 373 3859
AclI AA'CG_TT
Cuts at: 4072 4072
Size: 4232
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 1169 2057 2489 3236 3504 146
Size: 1023 888 432 747 268 874
Fragments arranged by size:
1023 888 874 747 432 268
AfeI AGC'GCT
Cuts at: 902 902
Size: 4232
AflIII A'CryG_T
Cuts at: 1584 2688 3665 1584
Size: 1104 977 2151
Fragments arranged by size:
2151 1104 977
AgeI A'CCGG_T
Cuts at: 2620 2620
Size: 4232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1187 1187
Size: 4232
AlwNI CAG_nnn'CTG
Cuts at: 225 328 601 703 739 1537 3104 225
Size: 103 273 102 36 798 1567 1353
Fragments arranged by size:
1567 1353 798 273 103 102 36
ApaI G_GGCC'C
Cuts at: 1041 1041
Size: 4232
ApaLI G'TGCA_C
Cuts at: 3002 3002
Size: 4232
ApoI r'AATT_y
Cuts at: 1236 3585 3841 1236
Size: 2349 256 1627
Fragments arranged by size:
2349 1627 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4232
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
AvaI C'yCGr_G
Cuts at: 1199 1252 1199
Size: 53 4179
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 139 172 139
Size: 33 4199
BamHI G'GATC_C
Cuts at: 1257 1257
Size: 4232
BanI G'GyrC_C
Cuts at: 875 1248 1662 1910 1945 875
Size: 373 414 248 35 3162
Fragments arranged by size:
3162 414 373 248 35
BanII G_rGCy'C
Cuts at: 257 362 728 1041 1246 2276 257
Size: 105 366 313 205 1030 2213
Fragments arranged by size:
2213 1030 366 313 205 105
BbeI G_GCGC'C
Cuts at: 1666 1914 1666
Size: 248 3984
BbsI GAAGACnn'nnnn_
Cuts at: 533 533
Size: 4232
BciVI GTATCCnnnnn_n'
Cuts at: 2130 2891 4232 2130
Size: 761 1341 2130
Fragments arranged by size:
2130 1341 761
BclI T'GATC_A
Cuts at: 1752 1752
Size: 4232
BglI GCCn_nnn'nGGC
Cuts at: 446 507 446
Size: 61 4171
BglII A'GATC_T
Cuts at: 1747 3784 1747
Size: 2037 2195
BlpI GC'TnA_GC
Cuts at: 749 1344 749
Size: 595 3637
Bme1580I G_kGCm'C
Cuts at: 1041 1199 1857 1950 3006 1041
Size: 158 658 93 1056 2267
Fragments arranged by size:
2267 1056 658 158 93
BmrI ACTGGGnnnn_n'
Cuts at: 631 1444 1525 1860 4004 631
Size: 813 81 335 2144 859
Fragments arranged by size:
2144 859 813 335 81
BmtI G_CTAG'C
Cuts at: 901 901
Size: 4232
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 747 2599 747
Size: 1852 2380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 1009 1021 1084 607
Size: 402 12 63 3755
Fragments arranged by size:
3755 402 63 12
Bpu10I CC'TnA_GC
Cuts at: 477 515 477
Size: 38 4194
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 1408 2287 2779 3077 601
Size: 807 879 492 298 1756
Fragments arranged by size:
1756 879 807 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 700 3936 700
Size: 3236 996
BsaAI yAC'GTr
Cuts at: 1585 1585
Size: 4232
BsaBI GATnn'nnATC
Cuts at: 6 1746 3783 6
Size: 1740 2037 455
Fragments arranged by size:
2037 1740 455
BsaHI Gr'CG_yC
Cuts at: 1663 1911 2613 4109 1663
Size: 248 702 1496 1786
Fragments arranged by size:
1786 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1055 1421 1942 2620 2894 3041 3452 1055
Size: 366 521 678 274 147 411 1835
Fragments arranged by size:
1835 678 521 411 366 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 187 217 187
Size: 30 4202
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 720 720
Size: 4232
BseYI C'CCAG_C
Cuts at: 125 264 363 1657 2415 2992 3488 125
Size: 139 99 1294 758 577 496 869
Fragments arranged by size:
1294 869 758 577 496 139 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 743 1003 743
Size: 260 3972
BsiEI CG_ry'CG
Cuts at: 104 1774 1820 3028 104
Size: 1670 46 1208 1308
Fragments arranged by size:
1670 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 362 1246 2024 2214 3006 362
Size: 884 778 190 792 1588
Fragments arranged by size:
1588 884 792 778 190
BsmI GAATG_Cn'
Cuts at: 234 234
Size: 4232
Bsp1286I G_dGCh'C
Cuts at: 257 362 728 1041 1199 1246 1857 1950 2024
Size: 105 366 313 158 47 611 93 74
Cuts at: 2024 2214 2276 3006 257
Size: 190 62 730 1483
Fragments arranged by size:
1483 730 611 366 313 190 158 105
93 74 62 47
BspEI T'CCGG_A
Cuts at: 3452 3452
Size: 4232
BspHI T'CATG_A
Cuts at: 3408 3408
Size: 4232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1282 1496 1798 2179 1282
Size: 214 302 381 3335
Fragments arranged by size:
3335 381 302 214
BsrBI CCG'CTC
Cuts at: 50 465 993 1005 1026 2524 2578 4062 4227
Size: 415 528 12 21 1498 54 1484 165
Cuts at: 4227 50
Size: 55
Fragments arranged by size:
1498 1484 528 415 165 55 54 21
12
BsrDI GCAATG_nn'
Cuts at: 2144 2144
Size: 4232
BsrFI r'CCGG_y
Cuts at: 843 2230 2411 2620 843
Size: 1387 181 209 2455
Fragments arranged by size:
2455 1387 209 181
BssSI C'ACGA_G
Cuts at: 2503 2861 2503
Size: 358 3874
BstAPI GCAn_nnn'nTGC
Cuts at: 1609 1609
Size: 4232
BstBI TT'CG_AA
Cuts at: 2593 2593
Size: 4232
BstXI CCAn_nnnn'nTGG
Cuts at: 672 672
Size: 4232
BstYI r'GATC_y
Cuts at: 1 387 1257 1724 1747 2082 2328 3340 3426
Size: 386 870 467 23 335 246 1012 86
Cuts at: 3426 3438 3784 1
Size: 12 346 449
Fragments arranged by size:
1012 870 467 449 386 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 732 732
Size: 4232
BtgI C'CryG_G
Cuts at: 1041 1041
Size: 4232
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 581 2182 2363 2526 581
Size: 1601 181 163 2287
Fragments arranged by size:
2287 1601 181 163
BtsI GCAGTG_nn'
Cuts at: 755 1475 755
Size: 720 3512
DraI TTT'AAA
Cuts at: 1231 1231
Size: 4232
DrdI GACnn_nn'nnGTC
Cuts at: 832 1938 2796 832
Size: 1106 858 2268
Fragments arranged by size:
2268 1106 858
EaeI y'GGCC_r
Cuts at: 413 800 1817 1991 2382 2409 3754 413
Size: 387 1017 174 391 27 1345 891
Fragments arranged by size:
1345 1017 891 391 387 174 27
EagI C'GGCC_G
Cuts at: 1817 1817
Size: 4232
EarI CTCTTCn'nnn_
Cuts at: 1145 2255 2465 1145
Size: 1110 210 2912
Fragments arranged by size:
2912 1110 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2440 2750 2896 4042 2440
Size: 310 146 1146 2630
Fragments arranged by size:
2630 1146 310 146
EcoICRI GAG'CTC
Cuts at: 360 1244 360
Size: 884 3348
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 607 1009 1021 1084 1169 2057 2489 3236
Size: 461 402 12 63 85 888 432 747
Cuts at: 3236 3504 146
Size: 268 874
Fragments arranged by size:
888 874 747 461 432 402 268 85
63 12
EcoO109I rG'GnC_Cy
Cuts at: 736 1037 1371 736
Size: 301 334 3597
Fragments arranged by size:
3597 334 301
EcoRI G'AATT_C
Cuts at: 1236 1236
Size: 4232
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2251 2251
Size: 4232
FspI TGC'GCA
Cuts at: 2013 2013
Size: 4232
HaeII r_GCGC'y
Cuts at: 904 1666 1914 2936 3915 904
Size: 762 248 1022 979 1221
Fragments arranged by size:
1221 1022 979 762 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 187 219 520 539 552 559 571 591 2074
Size: 32 301 19 13 7 12 20 1483
Cuts at: 2074 2104 2106 2136 187
Size: 30 2 30 2283
Fragments arranged by size:
2283 1483 301 32 30 30 20 19
13 12 7 2
HincII GTy'rAC
Cuts at: 837 1271 837
Size: 434 3798
KasI G'GCGC_C
Cuts at: 1662 1910 1662
Size: 248 3984
KpnI G_GTAC'C
Cuts at: 879 1252 879
Size: 373 3859
MluI A'CGCG_T
Cuts at: 3665 3665
Size: 4232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 563 707 942 2903 3087 563
Size: 144 235 1961 184 1708
Fragments arranged by size:
1961 1708 235 184 144
MscI TGG'CCA
Cuts at: 415 802 1993 415
Size: 387 1191 2654
Fragments arranged by size:
2654 1191 387
MslI CAynn'nnrTG
Cuts at: 2221 2348 2221
Size: 127 4105
MspA1I CmG'CkG
Cuts at: 153 700 882 987 1043 1083 1343 1657 2017
Size: 547 182 105 56 40 260 314 360
Cuts at: 2017 3030 3275 153
Size: 1013 245 1110
Fragments arranged by size:
1110 1013 547 360 314 260 245 182
105 56 40
NaeI GCC'GGC
Cuts at: 2413 2413
Size: 4232
NarI GG'CG_CC
Cuts at: 1663 1911 1663
Size: 248 3984
NgoMIV G'CCGG_C
Cuts at: 2411 2411
Size: 4232
NheI G'CTAG_C
Cuts at: 897 897
Size: 4232
NspI r_CATG'y
Cuts at: 1285 1785 2692 1285
Size: 500 907 2825
Fragments arranged by size:
2825 907 500
PciI A'CATG_T
Cuts at: 2688 2688
Size: 4232
PflMI CCAn_nnn'nTGG
Cuts at: 277 328 277
Size: 51 4181
PfoI T'CCnGG_A
Cuts at: 485 908 485
Size: 423 3809
PmeI GTTT'AAAC
Cuts at: 1231 1231
Size: 4232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3371 3403 3371
Size: 32 4200
PspOMI G'GGCC_C
Cuts at: 1037 1037
Size: 4232
PstI C_TGCA'G
Cuts at: 221 1279 1964 221
Size: 1058 685 2489
Fragments arranged by size:
2489 1058 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4232
PvuII CAG'CTG
Cuts at: 153 700 987 1083 1657 2017 153
Size: 547 287 96 574 360 2368
Fragments arranged by size:
2368 574 547 360 287 96
RsrII CG'GwC_CG
Cuts at: 2427 2427
Size: 4232
SacI G_AGCT'C
Cuts at: 362 1246 362
Size: 884 3348
SacII CC_GC'GG
Cuts at: 1044 1044
Size: 4232
SalI G'TCGA_C
Cuts at: 1269 1269
Size: 4232
SapI GCTCTTCn'nnn_
Cuts at: 2255 2465 2255
Size: 210 4022
SbfI CC_TGCA'GG
Cuts at: 1279 1279
Size: 4232
ScaI AGT'ACT
Cuts at: 3749 3749
Size: 4232
SfcI C'TryA_G
Cuts at: 33 217 561 1275 1960 2953 3144 33
Size: 184 344 714 685 993 191 1121
Fragments arranged by size:
1121 993 714 685 344 191 184
SfoI GGC'GCC
Cuts at: 1664 1912 1664
Size: 248 3984
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
SmaI CCC'GGG
Cuts at: 1201 1254 1201
Size: 53 4179
SmlI C'TyrA_G
Cuts at: 580 1387 2302 2794 3056 580
Size: 807 915 492 262 1756
Fragments arranged by size:
1756 915 807 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4232
SphI G_CATG'C
Cuts at: 1285 1785 1285
Size: 500 3732
StuI AGG'CCT
Cuts at: 449 449
Size: 4232
StyI C'CwwG_G
Cuts at: 276 671 713 797 1366 1720 276
Size: 395 42 84 569 354 2788
Fragments arranged by size:
2788 569 395 354 84 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 306 335 1815 1948 2407 2616 306
Size: 29 1480 133 459 209 1922
Fragments arranged by size:
1922 1480 459 209 133 29
TatI w'GTAC_w
Cuts at: 3747 3747
Size: 4232
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3749 3781 3749
Size: 32 4200
Tth111I GACn'n_nGTC
Cuts at: 2029 2029
Size: 4232
XbaI T'CTAG_A
Cuts at: 1263 1263
Size: 4232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 325 984 1727 325
Size: 659 743 2830
Fragments arranged by size:
2830 743 659
XmaI C'CCGG_G
Cuts at: 1199 1252 1199
Size: 53 4179
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NheI NspI
PciI PflMI PfoI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI AscI AvrII
BbvCI BcgI BfrBI BmgBI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NotI NruI
NsiI PacI PasI PmlI PpuMI PshAI PsiI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI