Restriction map for
pF1KB8956
(Circular) (Six-base) MAPSORT of: pF1KB8956.seq Check: 6495 from: 1 to: 4112
pF1KB8956 4112 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 843 1090 843
Size: 247 3865
AatII G_ACGT'C
Cuts at: 139 139
Size: 4112
AccI GT'mk_AC
Cuts at: 1150 1150
Size: 4112
Acc65I G'GTAC_C
Cuts at: 334 1128 334
Size: 794 3318
AclI AA'CG_TT
Cuts at: 3952 3952
Size: 4112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 856 1937 2369 3116 3384 856
Size: 1081 432 747 268 1584
Fragments arranged by size:
1584 1081 747 432 268
AfeI AGC'GCT
Cuts at: 730 793 730
Size: 63 4049
AflIII A'CryG_T
Cuts at: 624 626 1464 2568 3545 624
Size: 2 838 1104 977 1191
Fragments arranged by size:
1191 1104 977 838 2
AgeI A'CCGG_T
Cuts at: 2500 2500
Size: 4112
AlwNI CAG_nnn'CTG
Cuts at: 1417 2984 1417
Size: 1567 2545
ApaI G_GGCC'C
Cuts at: 809 809
Size: 4112
ApaLI G'TGCA_C
Cuts at: 620 2882 620
Size: 2262 1850
ApoI r'AATT_y
Cuts at: 1116 3465 3721 1116
Size: 2349 256 1507
Fragments arranged by size:
2349 1507 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4112
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
AvaI C'yCGr_G
Cuts at: 214 808 1132 214
Size: 594 324 3194
Fragments arranged by size:
3194 594 324
BamHI G'GATC_C
Cuts at: 469 1137 469
Size: 668 3444
BanI G'GyrC_C
Cuts at: 121 203 210 301 334 708 927 1099 1128
Size: 82 7 91 33 374 219 172 29
Cuts at: 1128 1542 1790 1825 121
Size: 414 248 35 2408
Fragments arranged by size:
2408 414 374 248 219 172 91 82
35 33 29 7
BanII G_rGCy'C
Cuts at: 370 809 1126 2156 370
Size: 439 317 1030 2326
Fragments arranged by size:
2326 1030 439 317
BbeI G_GCGC'C
Cuts at: 125 207 214 305 712 931 1546 1794 125
Size: 82 7 91 407 219 615 248 2443
Fragments arranged by size:
2443 615 407 248 219 91 82 7
BbsI GAAGACnn'nnnn_
Cuts at: 753 753
Size: 4112
BciVI GTATCCnnnnn_n'
Cuts at: 2010 2771 4112 2010
Size: 761 1341 2010
Fragments arranged by size:
2010 1341 761
BclI T'GATC_A
Cuts at: 1632 1632
Size: 4112
BglI GCCn_nnn'nGGC
Cuts at: 201 374 451 1064 201
Size: 173 77 613 3249
Fragments arranged by size:
3249 613 173 77
BglII A'GATC_T
Cuts at: 1627 3664 1627
Size: 2037 2075
BlpI GC'TnA_GC
Cuts at: 397 1224 397
Size: 827 3285
Bme1580I G_kGCm'C
Cuts at: 223 624 809 1737 1830 2886 223
Size: 401 185 928 93 1056 1449
Fragments arranged by size:
1449 1056 928 401 185 93
BmrI ACTGGGnnnn_n'
Cuts at: 1324 1405 1740 3884 1324
Size: 81 335 2144 1552
Fragments arranged by size:
2144 1552 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2479 2479
Size: 4112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 790 790
Size: 4112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 495 1288 2167 2659 2957 495
Size: 793 879 492 298 1650
Fragments arranged by size:
1650 879 793 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3816 3816
Size: 4112
BsaAI yAC'GTr
Cuts at: 627 1465 627
Size: 838 3274
BsaBI GATnn'nnATC
Cuts at: 6 1626 3663 6
Size: 1620 2037 455
Fragments arranged by size:
2037 1620 455
BsaHI Gr'CG_yC
Cuts at: 122 136 204 211 302 322 680 709 928
Size: 14 68 7 91 20 358 29 219
Cuts at: 928 1543 1791 2493 3989 122
Size: 615 248 702 1496 245
Fragments arranged by size:
1496 702 615 358 248 245 219 91
68 29 20 14 7
BsaWI w'CCGG_w
Cuts at: 1301 1822 2500 2774 2921 3332 1301
Size: 521 678 274 147 411 2081
Fragments arranged by size:
2081 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 373 403 373
Size: 30 4082
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 187 418 538 703 706 709 712 187
Size: 231 120 165 3 3 3 3587
Fragments arranged by size:
3587 231 165 120 3 3 3
BseYI C'CCAG_C
Cuts at: 377 1067 1537 2295 2872 3368 377
Size: 690 470 758 577 496 1121
Fragments arranged by size:
1121 758 690 577 496 470
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 660 660
Size: 4112
BsiEI CG_ry'CG
Cuts at: 104 579 1654 1700 2908 104
Size: 475 1075 46 1208 1308
Fragments arranged by size:
1308 1208 1075 475 46
BsiHKAI G_wGCw'C
Cuts at: 624 1126 1904 2094 2886 624
Size: 502 778 190 792 1850
Fragments arranged by size:
1850 792 778 502 190
BsmBI CGTCTCn'nnnn_
Cuts at: 329 573 841 329
Size: 244 268 3600
Fragments arranged by size:
3600 268 244
Bsp1286I G_dGCh'C
Cuts at: 223 370 624 809 1126 1737 1830 1904 2094
Size: 147 254 185 317 611 93 74 190
Cuts at: 2094 2156 2886 223
Size: 62 730 1449
Fragments arranged by size:
1449 730 611 317 254 190 185 147
93 74 62
BspEI T'CCGG_A
Cuts at: 3332 3332
Size: 4112
BspHI T'CATG_A
Cuts at: 3288 3288
Size: 4112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 747 843 923 995 1090 1162 1376 1678 2059
Size: 96 80 72 95 72 214 302 381
Cuts at: 2059 747
Size: 2800
Fragments arranged by size:
2800 381 302 214 96 95 80 72
72
BsrBI CCG'CTC
Cuts at: 50 2404 2458 3942 4107 50
Size: 2354 54 1484 165 55
Fragments arranged by size:
2354 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2024 2024
Size: 4112
BsrFI r'CCGG_y
Cuts at: 331 454 489 649 672 702 896 2110 2291
Size: 123 35 160 23 30 194 1214 181
Cuts at: 2291 2500 331
Size: 209 1943
Fragments arranged by size:
1943 1214 209 194 181 160 123 35
30 23
BssHII G'CGCG_C
Cuts at: 813 813
Size: 4112
BssSI C'ACGA_G
Cuts at: 2383 2741 2383
Size: 358 3754
BstAPI GCAn_nnn'nTGC
Cuts at: 1489 1489
Size: 4112
BstBI TT'CG_AA
Cuts at: 2473 2473
Size: 4112
BstXI CCAn_nnnn'nTGG
Cuts at: 945 945
Size: 4112
BstYI r'GATC_y
Cuts at: 1 469 1137 1604 1627 1962 2208 3220 3306
Size: 468 668 467 23 335 246 1012 86
Cuts at: 3306 3318 3664 1
Size: 12 346 449
Fragments arranged by size:
1012 668 468 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 239 533 107
Size: 132 294 3686
Fragments arranged by size:
3686 294 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2062 2243 2406 2062
Size: 181 163 3768
Fragments arranged by size:
3768 181 163
BtsI GCAGTG_nn'
Cuts at: 1024 1355 1024
Size: 331 3781
DraI TTT'AAA
Cuts at: 1111 1111
Size: 4112
DrdI GACnn_nn'nnGTC
Cuts at: 1818 2676 1818
Size: 858 3254
EaeI y'GGCC_r
Cuts at: 188 236 483 503 824 947 1006 1697 1871
Size: 48 247 20 321 123 59 691 174
Cuts at: 1871 2262 2289 3634 188
Size: 391 27 1345 666
Fragments arranged by size:
1345 691 666 391 321 247 174 123
59 48 27 20
EagI C'GGCC_G
Cuts at: 1697 1697
Size: 4112
EarI CTCTTCn'nnn_
Cuts at: 2135 2345 2135
Size: 210 3902
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 549 2320 2630 2776 3922 549
Size: 1771 310 146 1146 739
Fragments arranged by size:
1771 1146 739 310 146
EcoICRI GAG'CTC
Cuts at: 1124 1124
Size: 4112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 790 856 1937 2369 3116 3384 790
Size: 66 1081 432 747 268 1518
Fragments arranged by size:
1518 1081 747 432 268 66
EcoNI CCTnn'n_nnAGG
Cuts at: 523 523
Size: 4112
EcoO109I rG'GnC_Cy
Cuts at: 1251 1251
Size: 4112
EcoRI G'AATT_C
Cuts at: 1116 1116
Size: 4112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 4112
FseI GG_CCGG'CC
Cuts at: 458 458
Size: 4112
FspI TGC'GCA
Cuts at: 1086 1893 1086
Size: 807 3305
HaeII r_GCGC'y
Cuts at: 125 207 214 305 426 712 732 777 795
Size: 82 7 91 121 286 20 45 18
Cuts at: 795 897 931 1075 1546 1794 2816 3795 125
Size: 102 34 144 471 248 1022 979 442
Fragments arranged by size:
1022 979 471 442 286 248 144 121
102 91 82 45 34 20 18 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 373 405 1954 1984 1986 2016 373
Size: 32 1549 30 2 30 2469
Fragments arranged by size:
2469 1549 32 30 30 2
HincII GTy'rAC
Cuts at: 1151 1151
Size: 4112
KasI G'GCGC_C
Cuts at: 121 203 210 301 708 927 1542 1790 121
Size: 82 7 91 407 219 615 248 2443
Fragments arranged by size:
2443 615 407 248 219 91 82 7
KpnI G_GTAC'C
Cuts at: 338 1132 338
Size: 794 3318
MluI A'CGCG_T
Cuts at: 3545 3545
Size: 4112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2783 2967 2783
Size: 184 3928
MscI TGG'CCA
Cuts at: 1873 1873
Size: 4112
MslI CAynn'nnrTG
Cuts at: 112 2101 2228 112
Size: 1989 127 1996
Fragments arranged by size:
1996 1989 127
MspA1I CmG'CkG
Cuts at: 226 241 250 346 449 535 644 668 1090
Size: 15 9 96 103 86 109 24 422
Cuts at: 1090 1223 1537 1897 2910 3155 226
Size: 133 314 360 1013 245 1183
Fragments arranged by size:
1183 1013 422 360 314 245 133 109
103 96 86 24 15 9
NaeI GCC'GGC
Cuts at: 456 651 674 704 898 2293 456
Size: 195 23 30 194 1395 2275
Fragments arranged by size:
2275 1395 195 194 30 23
NarI GG'CG_CC
Cuts at: 122 204 211 302 709 928 1543 1791 122
Size: 82 7 91 407 219 615 248 2443
Fragments arranged by size:
2443 615 407 248 219 91 82 7
NcoI C'CATG_G
Cuts at: 107 107
Size: 4112
NgoMIV G'CCGG_C
Cuts at: 454 649 672 702 896 2291 454
Size: 195 23 30 194 1395 2275
Fragments arranged by size:
2275 1395 195 194 30 23
NspI r_CATG'y
Cuts at: 1165 1665 2572 1165
Size: 500 907 2705
Fragments arranged by size:
2705 907 500
PciI A'CATG_T
Cuts at: 2568 2568
Size: 4112
PmeI GTTT'AAAC
Cuts at: 1111 1111
Size: 4112
PmlI CAC'GTG
Cuts at: 627 627
Size: 4112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3251 3283 3251
Size: 32 4080
PspOMI G'GGCC_C
Cuts at: 805 805
Size: 4112
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 865 897 865
Size: 32 4080
PstI C_TGCA'G
Cuts at: 1031 1159 1844 1031
Size: 128 685 3299
Fragments arranged by size:
3299 685 128
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4112
PvuII CAG'CTG
Cuts at: 346 1090 1537 1897 346
Size: 744 447 360 2561
Fragments arranged by size:
2561 744 447 360
RsrII CG'GwC_CG
Cuts at: 2307 2307
Size: 4112
SacI G_AGCT'C
Cuts at: 1126 1126
Size: 4112
SacII CC_GC'GG
Cuts at: 242 536 242
Size: 294 3818
SalI G'TCGA_C
Cuts at: 1149 1149
Size: 4112
SapI GCTCTTCn'nnn_
Cuts at: 2135 2345 2135
Size: 210 3902
SbfI CC_TGCA'GG
Cuts at: 1159 1159
Size: 4112
ScaI AGT'ACT
Cuts at: 3629 3629
Size: 4112
SfcI C'TryA_G
Cuts at: 33 1027 1155 1840 2833 3024 33
Size: 994 128 685 993 191 1121
Fragments arranged by size:
1121 994 993 685 191 128
SfiI GGCCn_nnn'nGGCC
Cuts at: 451 451
Size: 4112
SfoI GGC'GCC
Cuts at: 123 205 212 303 710 929 1544 1792 123
Size: 82 7 91 407 219 615 248 2443
Fragments arranged by size:
2443 615 407 248 219 91 82 7
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
SmaI CCC'GGG
Cuts at: 216 1134 216
Size: 918 3194
SmlI C'TyrA_G
Cuts at: 474 1267 2182 2674 2936 474
Size: 793 915 492 262 1650
Fragments arranged by size:
1650 915 793 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4112
SphI G_CATG'C
Cuts at: 1165 1665 1165
Size: 500 3612
StyI C'CwwG_G
Cuts at: 107 586 1246 1600 107
Size: 479 660 354 2619
Fragments arranged by size:
2619 660 479 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 607 1117 1695 1828 2287 2496 607
Size: 510 578 133 459 209 2223
Fragments arranged by size:
2223 578 510 459 209 133
TatI w'GTAC_w
Cuts at: 3627 3627
Size: 4112
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3629 3661 3629
Size: 32 4080
Tth111I GACn'n_nGTC
Cuts at: 1909 1909
Size: 4112
XbaI T'CTAG_A
Cuts at: 1143 1143
Size: 4112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1607 1607
Size: 4112
XmaI C'CCGG_G
Cuts at: 214 1132 214
Size: 918 3194
ZraI GAC'GTC
Cuts at: 137 137
Size: 4112
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PmlI PpiI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AflII AhdI AleI AlfI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsiWI BsmI
BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PpuMI PshAI PsiI
PspXI SanDI SexAI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI