Restriction map for
pF1KB8950
(Circular) (Six-base) MAPSORT of: pF1KB8950.seq Check: 3912 from: 1 to: 4085
pF1KB8950 4085 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 1123 1123
Size: 4085
Acc65I G'GTAC_C
Cuts at: 590 1101 590
Size: 511 3574
AclI AA'CG_TT
Cuts at: 3925 3925
Size: 4085
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1910 2342 3089 3357 1910
Size: 432 747 268 2638
Fragments arranged by size:
2638 747 432 268
AflIII A'CryG_T
Cuts at: 1437 2541 3518 1437
Size: 1104 977 2004
Fragments arranged by size:
2004 1104 977
AgeI A'CCGG_T
Cuts at: 2473 2473
Size: 4085
AlwNI CAG_nnn'CTG
Cuts at: 711 1390 2957 711
Size: 679 1567 1839
Fragments arranged by size:
1839 1567 679
ApaI G_GGCC'C
Cuts at: 284 284
Size: 4085
ApaLI G'TGCA_C
Cuts at: 2855 2855
Size: 4085
ApoI r'AATT_y
Cuts at: 1089 3438 3694 1089
Size: 2349 256 1480
Fragments arranged by size:
2349 1480 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4085
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4085
AvaI C'yCGr_G
Cuts at: 283 506 1024 1044 1105 283
Size: 223 518 20 61 3263
Fragments arranged by size:
3263 518 223 61 20
AvrII C'CTAG_G
Cuts at: 798 798
Size: 4085
BamHI G'GATC_C
Cuts at: 1110 1110
Size: 4085
BanI G'GyrC_C
Cuts at: 231 590 765 810 1101 1515 1763 1798 231
Size: 359 175 45 291 414 248 35 2518
Fragments arranged by size:
2518 414 359 291 248 175 45 35
BanII G_rGCy'C
Cuts at: 206 284 1024 1099 2129 206
Size: 78 740 75 1030 2162
Fragments arranged by size:
2162 1030 740 78 75
BbeI G_GCGC'C
Cuts at: 235 814 1519 1767 235
Size: 579 705 248 2553
Fragments arranged by size:
2553 705 579 248
BbsI GAAGACnn'nnnn_
Cuts at: 484 484
Size: 4085
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 274 308 274
Size: 34 4051
BciVI GTATCCnnnnn_n'
Cuts at: 1078 1983 2744 4085 1078
Size: 905 761 1341 1078
Fragments arranged by size:
1341 1078 905 761
BclI T'GATC_A
Cuts at: 1605 1605
Size: 4085
BglII A'GATC_T
Cuts at: 656 1600 3637 656
Size: 944 2037 1104
Fragments arranged by size:
2037 1104 944
BlpI GC'TnA_GC
Cuts at: 1197 1197
Size: 4085
Bme1580I G_kGCm'C
Cuts at: 284 1710 1803 2859 284
Size: 1426 93 1056 1510
Fragments arranged by size:
1510 1426 1056 93
BmgBI CAC'GTC
Cuts at: 641 641
Size: 4085
BmrI ACTGGGnnnn_n'
Cuts at: 1297 1378 1713 3857 1297
Size: 81 335 2144 1525
Fragments arranged by size:
2144 1525 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2452 2452
Size: 4085
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 493 586 733 238
Size: 255 93 147 3590
Fragments arranged by size:
3590 255 147 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1261 2140 2632 2930 1261
Size: 879 492 298 2416
Fragments arranged by size:
2416 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3789 3789
Size: 4085
BsaAI yAC'GTr
Cuts at: 391 1438 391
Size: 1047 3038
BsaBI GATnn'nnATC
Cuts at: 6 1599 3636 6
Size: 1593 2037 455
Fragments arranged by size:
2037 1593 455
BsaHI Gr'CG_yC
Cuts at: 232 811 995 1516 1764 2466 3962 232
Size: 579 184 521 248 702 1496 355
Fragments arranged by size:
1496 702 579 521 355 248 184
BsaWI w'CCGG_w
Cuts at: 1274 1795 2473 2747 2894 3305 1274
Size: 521 678 274 147 411 2054
Fragments arranged by size:
2054 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 228 228
Size: 4085
BseYI C'CCAG_C
Cuts at: 115 277 324 411 945 1510 2268 2845 3341
Size: 162 47 87 534 565 758 577 496
Cuts at: 3341 115
Size: 859
Fragments arranged by size:
859 758 577 565 534 496 162 87
47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 900 900
Size: 4085
BsiEI CG_ry'CG
Cuts at: 104 1627 1673 2881 104
Size: 1523 46 1208 1308
Fragments arranged by size:
1523 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 206 539 1099 1877 2067 2859 206
Size: 333 560 778 190 792 1432
Fragments arranged by size:
1432 792 778 560 333 190
BsmI GAATG_Cn'
Cuts at: 1014 1014
Size: 4085
Bsp1286I G_dGCh'C
Cuts at: 206 284 539 1024 1099 1710 1803 1877 2067
Size: 78 255 485 75 611 93 74 190
Cuts at: 2067 2129 2859 206
Size: 62 730 1432
Fragments arranged by size:
1432 730 611 485 255 190 93 78
75 74 62
BspEI T'CCGG_A
Cuts at: 3305 3305
Size: 4085
BspHI T'CATG_A
Cuts at: 3261 3261
Size: 4085
BspMI ACCTGCnnnn'nnnn_
Cuts at: 543 639 1135 1349 1651 2032 543
Size: 96 496 214 302 381 2596
Fragments arranged by size:
2596 496 381 302 214 96
BsrBI CCG'CTC
Cuts at: 50 501 570 2377 2431 3915 4080 50
Size: 451 69 1807 54 1484 165 55
Fragments arranged by size:
1807 1484 451 165 69 55 54
BsrDI GCAATG_nn'
Cuts at: 1997 1997
Size: 4085
BsrFI r'CCGG_y
Cuts at: 342 671 954 2083 2264 2473 342
Size: 329 283 1129 181 209 1954
Fragments arranged by size:
1954 1129 329 283 209 181
BssHII G'CGCG_C
Cuts at: 315 332 449 315
Size: 17 117 3951
Fragments arranged by size:
3951 117 17
BssSI C'ACGA_G
Cuts at: 2356 2714 2356
Size: 358 3727
BstAPI GCAn_nnn'nTGC
Cuts at: 794 1462 794
Size: 668 3417
BstBI TT'CG_AA
Cuts at: 2446 2446
Size: 4085
BstYI r'GATC_y
Cuts at: 1 656 1110 1577 1600 1935 2181 3193 3279
Size: 655 454 467 23 335 246 1012 86
Cuts at: 3279 3291 3637 1
Size: 12 346 449
Fragments arranged by size:
1012 655 467 454 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 797 2035 2216 2379 797
Size: 1238 181 163 2503
Fragments arranged by size:
2503 1238 181 163
BtsI GCAGTG_nn'
Cuts at: 917 959 1328 917
Size: 42 369 3674
Fragments arranged by size:
3674 369 42
DraI TTT'AAA
Cuts at: 1084 1084
Size: 4085
DrdI GACnn_nn'nnGTC
Cuts at: 1791 2649 1791
Size: 858 3227
EaeI y'GGCC_r
Cuts at: 251 344 620 956 1670 1844 2235 2262 3607
Size: 93 276 336 714 174 391 27 1345
Cuts at: 3607 251
Size: 729
Fragments arranged by size:
1345 729 714 391 336 276 174 93
27
EagI C'GGCC_G
Cuts at: 1670 1670
Size: 4085
EarI CTCTTCn'nnn_
Cuts at: 544 2108 2318 544
Size: 1564 210 2311
Fragments arranged by size:
2311 1564 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 502 534 2293 2603 2749 3895 502
Size: 32 1759 310 146 1146 692
Fragments arranged by size:
1759 1146 692 310 146 32
EcoICRI GAG'CTC
Cuts at: 204 1097 204
Size: 893 3192
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 493 586 733 1910 2342 3089 3357 238
Size: 255 93 147 1177 432 747 268 966
Fragments arranged by size:
1177 966 747 432 268 255 147 93
EcoO109I rG'GnC_Cy
Cuts at: 420 1224 420
Size: 804 3281
EcoRI G'AATT_C
Cuts at: 1089 1089
Size: 4085
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2104 2104
Size: 4085
FspI TGC'GCA
Cuts at: 1866 1866
Size: 4085
HaeII r_GCGC'y
Cuts at: 235 576 678 814 1519 1767 2789 3768 235
Size: 341 102 136 705 248 1022 979 552
Fragments arranged by size:
1022 979 705 552 341 248 136 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1927 1957 1959 1989 1927
Size: 30 2 30 4023
Fragments arranged by size:
4023 30 30 2
HincII GTy'rAC
Cuts at: 1124 1124
Size: 4085
KasI G'GCGC_C
Cuts at: 231 810 1515 1763 231
Size: 579 705 248 2553
Fragments arranged by size:
2553 705 579 248
KpnI G_GTAC'C
Cuts at: 594 1105 594
Size: 511 3574
MluI A'CGCG_T
Cuts at: 3518 3518
Size: 4085
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2756 2940 2756
Size: 184 3901
MscI TGG'CCA
Cuts at: 622 1846 622
Size: 1224 2861
MslI CAynn'nnrTG
Cuts at: 2074 2201 2074
Size: 127 3958
MspA1I CmG'CkG
Cuts at: 588 618 696 914 1196 1510 1870 2883 3128
Size: 30 78 218 282 314 360 1013 245
Cuts at: 3128 588
Size: 1545
Fragments arranged by size:
1545 1013 360 314 282 245 218 78
30
NaeI GCC'GGC
Cuts at: 956 2266 956
Size: 1310 2775
NarI GG'CG_CC
Cuts at: 232 811 1516 1764 232
Size: 579 705 248 2553
Fragments arranged by size:
2553 705 579 248
NgoMIV G'CCGG_C
Cuts at: 954 2264 954
Size: 1310 2775
NspI r_CATG'y
Cuts at: 249 1138 1638 2545 249
Size: 889 500 907 1789
Fragments arranged by size:
1789 907 889 500
PciI A'CATG_T
Cuts at: 2541 2541
Size: 4085
PflMI CCAn_nnn'nTGG
Cuts at: 711 711
Size: 4085
PmeI GTTT'AAAC
Cuts at: 1084 1084
Size: 4085
PmlI CAC'GTG
Cuts at: 391 391
Size: 4085
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3224 3256 3224
Size: 32 4053
PpuMI rG'GwC_Cy
Cuts at: 420 420
Size: 4085
PsiI TTA'TAA
Cuts at: 857 857
Size: 4085
PspOMI G'GGCC_C
Cuts at: 280 280
Size: 4085
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 661 693 661
Size: 32 4053
PstI C_TGCA'G
Cuts at: 458 901 1132 1817 458
Size: 443 231 685 2726
Fragments arranged by size:
2726 685 443 231
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4085
PvuII CAG'CTG
Cuts at: 618 914 1510 1870 618
Size: 296 596 360 2833
Fragments arranged by size:
2833 596 360 296
RsrII CG'GwC_CG
Cuts at: 2280 2280
Size: 4085
SacI G_AGCT'C
Cuts at: 206 1099 206
Size: 893 3192
SalI G'TCGA_C
Cuts at: 1122 1122
Size: 4085
SapI GCTCTTCn'nnn_
Cuts at: 544 2108 2318 544
Size: 1564 210 2311
Fragments arranged by size:
2311 1564 210
SbfI CC_TGCA'GG
Cuts at: 1132 1132
Size: 4085
ScaI AGT'ACT
Cuts at: 3602 3602
Size: 4085
SfcI C'TryA_G
Cuts at: 33 396 454 897 909 1128 1813 2806 2997
Size: 363 58 443 12 219 685 993 191
Cuts at: 2997 33
Size: 1121
Fragments arranged by size:
1121 993 685 443 363 219 191 58
12
SfoI GGC'GCC
Cuts at: 233 812 1517 1765 233
Size: 579 705 248 2553
Fragments arranged by size:
2553 705 579 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4085
SmaI CCC'GGG
Cuts at: 508 1026 1107 508
Size: 518 81 3486
Fragments arranged by size:
3486 518 81
SmlI C'TyrA_G
Cuts at: 1240 2155 2647 2909 1240
Size: 915 492 262 2416
Fragments arranged by size:
2416 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4085
SphI G_CATG'C
Cuts at: 249 1138 1638 249
Size: 889 500 2696
Fragments arranged by size:
2696 889 500
StuI AGG'CCT
Cuts at: 271 271
Size: 4085
StyI C'CwwG_G
Cuts at: 416 798 1219 1573 416
Size: 382 421 354 2928
Fragments arranged by size:
2928 421 382 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1668 1801 2260 2469 1668
Size: 133 459 209 3284
Fragments arranged by size:
3284 459 209 133
TatI w'GTAC_w
Cuts at: 3600 3600
Size: 4085
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3602 3634 3602
Size: 32 4053
Tth111I GACn'n_nGTC
Cuts at: 1882 1882
Size: 4085
XbaI T'CTAG_A
Cuts at: 1116 1116
Size: 4085
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1580 1580
Size: 4085
XmaI C'CCGG_G
Cuts at: 506 1024 1105 506
Size: 518 81 3486
Fragments arranged by size:
3486 518 81
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PmeI PmlI PpiI PpuMI PsiI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI BaeI BbvCI BfrBI BglI BmtI Bpu10I BsaXI
BsiWI BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PshAI PspXI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI