Restriction map for
pF1KB8940
(Circular) (Six-base) MAPSORT of: pF1KB8940.seq Check: 1240 from: 1 to: 4004
pF1KB8940 4004 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 723 723
Size: 4004
AccI GT'mk_AC
Cuts at: 1042 1042
Size: 4004
Acc65I G'GTAC_C
Cuts at: 1020 1020
Size: 4004
AclI AA'CG_TT
Cuts at: 3844 3844
Size: 4004
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 255 823 1829 2261 3008 3276 255
Size: 568 1006 432 747 268 983
Fragments arranged by size:
1006 983 747 568 432 268
AflIII A'CryG_T
Cuts at: 1356 2460 3437 1356
Size: 1104 977 1923
Fragments arranged by size:
1923 1104 977
AgeI A'CCGG_T
Cuts at: 2392 2392
Size: 4004
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 643 675 643
Size: 32 3972
AlwNI CAG_nnn'CTG
Cuts at: 375 642 678 1309 2876 375
Size: 267 36 631 1567 1503
Fragments arranged by size:
1567 1503 631 267 36
ApaLI G'TGCA_C
Cuts at: 2774 2774
Size: 4004
ApoI r'AATT_y
Cuts at: 1008 3357 3613 1008
Size: 2349 256 1399
Fragments arranged by size:
2349 1399 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4004
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
AvaI C'yCGr_G
Cuts at: 499 508 1024 499
Size: 9 516 3479
Fragments arranged by size:
3479 516 9
BamHI G'GATC_C
Cuts at: 1029 1029
Size: 4004
BanI G'GyrC_C
Cuts at: 330 892 907 945 1020 1434 1682 1717 330
Size: 562 15 38 75 414 248 35 2617
Fragments arranged by size:
2617 562 414 248 75 38 35 15
BanII G_rGCy'C
Cuts at: 405 499 535 571 1018 2048 405
Size: 94 36 36 447 1030 2361
Fragments arranged by size:
2361 1030 447 94 36 36
BbeI G_GCGC'C
Cuts at: 334 949 1438 1686 334
Size: 615 489 248 2652
Fragments arranged by size:
2652 615 489 248
BciVI GTATCCnnnnn_n'
Cuts at: 1902 2663 4004 1902
Size: 761 1341 1902
Fragments arranged by size:
1902 1341 761
BclI T'GATC_A
Cuts at: 1524 1524
Size: 4004
BglI GCCn_nnn'nGGC
Cuts at: 199 558 199
Size: 359 3645
BglII A'GATC_T
Cuts at: 1519 3556 1519
Size: 2037 1967
BlpI GC'TnA_GC
Cuts at: 565 715 1116 565
Size: 150 401 3453
Fragments arranged by size:
3453 401 150
Bme1580I G_kGCm'C
Cuts at: 1629 1722 2778 1629
Size: 93 1056 2855
Fragments arranged by size:
2855 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 385 1216 1297 1632 3776 385
Size: 831 81 335 2144 613
Fragments arranged by size:
2144 831 613 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2371 2371
Size: 4004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 690 814 670
Size: 20 124 3860
Fragments arranged by size:
3860 124 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 1180 2059 2551 2849 445
Size: 735 879 492 298 1600
Fragments arranged by size:
1600 879 735 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3708 3708
Size: 4004
BsaAI yAC'GTr
Cuts at: 1357 1357
Size: 4004
BsaBI GATnn'nnATC
Cuts at: 6 1518 3555 6
Size: 1512 2037 455
Fragments arranged by size:
2037 1512 455
BsaHI Gr'CG_yC
Cuts at: 331 380 619 946 1435 1683 2385 3881 331
Size: 49 239 327 489 248 702 1496 454
Fragments arranged by size:
1496 702 489 454 327 248 239 49
BsaWI w'CCGG_w
Cuts at: 1193 1714 2392 2666 2813 3224 1193
Size: 521 678 274 147 411 1973
Fragments arranged by size:
1973 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 643 673 643
Size: 30 3974
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 244 448 244
Size: 204 3800
BseYI C'CCAG_C
Cuts at: 187 396 573 1429 2187 2764 3260 187
Size: 209 177 856 758 577 496 931
Fragments arranged by size:
931 856 758 577 496 209 177
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 564 367
Size: 197 3807
BsiEI CG_ry'CG
Cuts at: 104 430 884 1546 1592 2800 104
Size: 326 454 662 46 1208 1308
Fragments arranged by size:
1308 1208 662 454 326 46
BsiHKAI G_wGCw'C
Cuts at: 1018 1796 1986 2778 1018
Size: 778 190 792 2244
Fragments arranged by size:
2244 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 721 721
Size: 4004
Bsp1286I G_dGCh'C
Cuts at: 405 499 535 571 1018 1629 1722 1796 1986
Size: 94 36 36 447 611 93 74 190
Cuts at: 1986 2048 2778 405
Size: 62 730 1631
Fragments arranged by size:
1631 730 611 447 190 94 93 74
62 36 36
BspEI T'CCGG_A
Cuts at: 3224 3224
Size: 4004
BspHI T'CATG_A
Cuts at: 3180 3180
Size: 4004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 723 1054 1268 1570 1951 723
Size: 331 214 302 381 2776
Fragments arranged by size:
2776 381 331 302 214
BsrBI CCG'CTC
Cuts at: 50 432 2296 2350 3834 3999 50
Size: 382 1864 54 1484 165 55
Fragments arranged by size:
1864 1484 382 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1916 1916
Size: 4004
BsrFI r'CCGG_y
Cuts at: 159 552 831 2002 2183 2392 159
Size: 393 279 1171 181 209 1771
Fragments arranged by size:
1771 1171 393 279 209 181
BssHII G'CGCG_C
Cuts at: 693 927 693
Size: 234 3770
BssSI C'ACGA_G
Cuts at: 2275 2633 2275
Size: 358 3646
BstAPI GCAn_nnn'nTGC
Cuts at: 1381 1381
Size: 4004
BstBI TT'CG_AA
Cuts at: 2365 2365
Size: 4004
BstYI r'GATC_y
Cuts at: 1 1029 1496 1519 1854 2100 3112 3198 3210
Size: 1028 467 23 335 246 1012 86 12
Cuts at: 3210 3556 1
Size: 346 449
Fragments arranged by size:
1028 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 197 203 215 424 520 613 884 950 197
Size: 6 12 209 96 93 271 66 3251
Fragments arranged by size:
3251 271 209 96 93 66 12 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 669 1954 2135 2298 669
Size: 1285 181 163 2375
Fragments arranged by size:
2375 1285 181 163
BtsI GCAGTG_nn'
Cuts at: 1247 1247
Size: 4004
DraI TTT'AAA
Cuts at: 1003 1003
Size: 4004
DraIII CAC_nnn'GTG
Cuts at: 127 127
Size: 4004
DrdI GACnn_nn'nnGTC
Cuts at: 1710 2568 1710
Size: 858 3146
EaeI y'GGCC_r
Cuts at: 200 290 427 435 554 809 881 1589 1763
Size: 90 137 8 119 255 72 708 174
Cuts at: 1763 2154 2181 3526 200
Size: 391 27 1345 678
Fragments arranged by size:
1345 708 678 391 255 174 137 119
90 72 27 8
EagI C'GGCC_G
Cuts at: 427 881 1589 427
Size: 454 708 2842
Fragments arranged by size:
2842 708 454
EarI CTCTTCn'nnn_
Cuts at: 423 2027 2237 423
Size: 1604 210 2190
Fragments arranged by size:
2190 1604 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 135 652 2212 2522 2668 3814 135
Size: 517 1560 310 146 1146 325
Fragments arranged by size:
1560 1146 517 325 310 146
EcoICRI GAG'CTC
Cuts at: 1016 1016
Size: 4004
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 255 670 690 814 823 1829 2261 3008 3276
Size: 415 20 124 9 1006 432 747 268
Cuts at: 3276 255
Size: 983
Fragments arranged by size:
1006 983 747 432 415 268 124 20
9
EcoO109I rG'GnC_Cy
Cuts at: 183 515 1143 183
Size: 332 628 3044
Fragments arranged by size:
3044 628 332
EcoRI G'AATT_C
Cuts at: 1008 1008
Size: 4004
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2023 2023
Size: 4004
FspI TGC'GCA
Cuts at: 1785 1785
Size: 4004
HaeII r_GCGC'y
Cuts at: 194 286 326 334 649 949 1438 1686 2708
Size: 92 40 8 315 300 489 248 1022
Cuts at: 2708 3687 194
Size: 979 511
Fragments arranged by size:
1022 979 511 489 315 300 248 92
40 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 400 432 1846 1876 1878 1908 400
Size: 32 1414 30 2 30 2496
Fragments arranged by size:
2496 1414 32 30 30 2
HincII GTy'rAC
Cuts at: 1043 1043
Size: 4004
KasI G'GCGC_C
Cuts at: 330 945 1434 1682 330
Size: 615 489 248 2652
Fragments arranged by size:
2652 615 489 248
KpnI G_GTAC'C
Cuts at: 1024 1024
Size: 4004
MluI A'CGCG_T
Cuts at: 3437 3437
Size: 4004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2675 2859 2675
Size: 184 3820
MscI TGG'CCA
Cuts at: 1765 1765
Size: 4004
MslI CAynn'nnrTG
Cuts at: 1993 2120 1993
Size: 127 3877
MspA1I CmG'CkG
Cuts at: 199 426 615 639 874 886 1115 1429 1789
Size: 227 189 24 235 12 229 314 360
Cuts at: 1789 2802 3047 199
Size: 1013 245 1156
Fragments arranged by size:
1156 1013 360 314 245 235 229 227
189 24 12
NaeI GCC'GGC
Cuts at: 161 554 2185 161
Size: 393 1631 1980
Fragments arranged by size:
1980 1631 393
NarI GG'CG_CC
Cuts at: 331 946 1435 1683 331
Size: 615 489 248 2652
Fragments arranged by size:
2652 615 489 248
NcoI C'CATG_G
Cuts at: 215 215
Size: 4004
NgoMIV G'CCGG_C
Cuts at: 159 552 2183 159
Size: 393 1631 1980
Fragments arranged by size:
1980 1631 393
NotI GC'GGCC_GC
Cuts at: 427 881 427
Size: 454 3550
NspI r_CATG'y
Cuts at: 987 1057 1557 2464 987
Size: 70 500 907 2527
Fragments arranged by size:
2527 907 500 70
PciI A'CATG_T
Cuts at: 2460 2460
Size: 4004
PmeI GTTT'AAAC
Cuts at: 1003 1003
Size: 4004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 643 675 3143 3175 643
Size: 32 2468 32 1472
Fragments arranged by size:
2468 1472 32 32
PpuMI rG'GwC_Cy
Cuts at: 515 515
Size: 4004
PstI C_TGCA'G
Cuts at: 1051 1736 1051
Size: 685 3319
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4004
PvuII CAG'CTG
Cuts at: 639 874 1429 1789 639
Size: 235 555 360 2854
Fragments arranged by size:
2854 555 360 235
RsrII CG'GwC_CG
Cuts at: 2199 2199
Size: 4004
SacI G_AGCT'C
Cuts at: 1018 1018
Size: 4004
SacII CC_GC'GG
Cuts at: 200 427 616 887 200
Size: 227 189 271 3317
Fragments arranged by size:
3317 271 227 189
SalI G'TCGA_C
Cuts at: 1041 1041
Size: 4004
SapI GCTCTTCn'nnn_
Cuts at: 2027 2237 2027
Size: 210 3794
SbfI CC_TGCA'GG
Cuts at: 1051 1051
Size: 4004
ScaI AGT'ACT
Cuts at: 3521 3521
Size: 4004
SfcI C'TryA_G
Cuts at: 33 875 978 1047 1732 2725 2916 33
Size: 842 103 69 685 993 191 1121
Fragments arranged by size:
1121 993 842 685 191 103 69
SfoI GGC'GCC
Cuts at: 332 947 1436 1684 332
Size: 615 489 248 2652
Fragments arranged by size:
2652 615 489 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
SmaI CCC'GGG
Cuts at: 1026 1026
Size: 4004
SmlI C'TyrA_G
Cuts at: 460 1159 2074 2566 2828 460
Size: 699 915 492 262 1636
Fragments arranged by size:
1636 915 699 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4004
SphI G_CATG'C
Cuts at: 1057 1557 1057
Size: 500 3504
StyI C'CwwG_G
Cuts at: 215 314 764 1138 1492 215
Size: 99 450 374 354 2727
Fragments arranged by size:
2727 450 374 354 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1587 1720 2179 2388 1587
Size: 133 459 209 3203
Fragments arranged by size:
3203 459 209 133
TatI w'GTAC_w
Cuts at: 3519 3519
Size: 4004
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 365 397 3521 3553 365
Size: 32 3124 32 816
Fragments arranged by size:
3124 816 32 32
Tth111I GACn'n_nGTC
Cuts at: 1801 1801
Size: 4004
XbaI T'CTAG_A
Cuts at: 1035 1035
Size: 4004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1499 1499
Size: 4004
XmaI C'CCGG_G
Cuts at: 1024 1024
Size: 4004
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsiWI BsmI BsrGI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI