Restriction map for
pF1KB8928
(Circular) (Six-base) MAPSORT of: pF1KB8928.seq Check: 6921 from: 1 to: 3953
pF1KB8928 3953 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 190 190
Size: 3953
AccI GT'mk_AC
Cuts at: 305 351 991 305
Size: 46 640 3267
Fragments arranged by size:
3267 640 46
Acc65I G'GTAC_C
Cuts at: 969 969
Size: 3953
AclI AA'CG_TT
Cuts at: 283 3793 283
Size: 3510 443
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1778 2210 2957 3225 1778
Size: 432 747 268 2506
Fragments arranged by size:
2506 747 432 268
AflIII A'CryG_T
Cuts at: 242 1305 2409 3386 242
Size: 1063 1104 977 809
Fragments arranged by size:
1104 1063 977 809
AgeI A'CCGG_T
Cuts at: 2341 2341
Size: 3953
AlwNI CAG_nnn'CTG
Cuts at: 132 1258 2825 132
Size: 1126 1567 1260
Fragments arranged by size:
1567 1260 1126
ApaLI G'TGCA_C
Cuts at: 2723 2723
Size: 3953
ApoI r'AATT_y
Cuts at: 957 3306 3562 957
Size: 2349 256 1348
Fragments arranged by size:
2349 1348 256
AseI AT'TA_AT
Cuts at: 20 380 20
Size: 360 3593
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3953
AvaI C'yCGr_G
Cuts at: 973 973
Size: 3953
BamHI G'GATC_C
Cuts at: 978 978
Size: 3953
BanI G'GyrC_C
Cuts at: 969 1383 1631 1666 969
Size: 414 248 35 3256
Fragments arranged by size:
3256 414 248 35
BanII G_rGCy'C
Cuts at: 967 1997 967
Size: 1030 2923
BbeI G_GCGC'C
Cuts at: 1387 1635 1387
Size: 248 3705
BbsI GAAGACnn'nnnn_
Cuts at: 564 626 564
Size: 62 3891
BciVI GTATCCnnnnn_n'
Cuts at: 1851 2612 3953 1851
Size: 761 1341 1851
Fragments arranged by size:
1851 1341 761
BclI T'GATC_A
Cuts at: 630 1473 630
Size: 843 3110
BglII A'GATC_T
Cuts at: 547 1468 3505 547
Size: 921 2037 995
Fragments arranged by size:
2037 995 921
BlpI GC'TnA_GC
Cuts at: 1065 1065
Size: 3953
Bme1580I G_kGCm'C
Cuts at: 1578 1671 2727 1578
Size: 93 1056 2804
Fragments arranged by size:
2804 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1165 1246 1581 3725 1165
Size: 81 335 2144 1393
Fragments arranged by size:
2144 1393 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 486 2320 486
Size: 1834 2119
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 349
Size: 3953
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 1129 2008 2500 2798 223
Size: 906 879 492 298 1378
Fragments arranged by size:
1378 906 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3657 3657
Size: 3953
BsaAI yAC'GTr
Cuts at: 189 1306 189
Size: 1117 2836
BsaBI GATnn'nnATC
Cuts at: 6 1467 3504 6
Size: 1461 2037 455
Fragments arranged by size:
2037 1461 455
BsaHI Gr'CG_yC
Cuts at: 1384 1632 2334 3830 1384
Size: 248 702 1496 1507
Fragments arranged by size:
1507 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1142 1663 2341 2615 2762 3173 1142
Size: 521 678 274 147 411 1922
Fragments arranged by size:
1922 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1378 2136 2713 3209 1378
Size: 758 577 496 2122
Fragments arranged by size:
2122 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 900 211
Size: 689 3264
BsiEI CG_ry'CG
Cuts at: 104 1495 1541 2749 104
Size: 1391 46 1208 1308
Fragments arranged by size:
1391 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 375 967 1745 1935 2727 375
Size: 592 778 190 792 1601
Fragments arranged by size:
1601 792 778 592 190
Bsp1286I G_dGCh'C
Cuts at: 375 967 1578 1671 1745 1935 1997 2727 375
Size: 592 611 93 74 190 62 730 1601
Fragments arranged by size:
1601 730 611 592 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3173 3173
Size: 3953
BspHI T'CATG_A
Cuts at: 3129 3129
Size: 3953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 190 1003 1217 1519 1900 190
Size: 813 214 302 381 2243
Fragments arranged by size:
2243 813 381 302 214
BsrBI CCG'CTC
Cuts at: 50 165 2245 2299 3783 3948 50
Size: 115 2080 54 1484 165 55
Fragments arranged by size:
2080 1484 165 115 55 54
BsrDI GCAATG_nn'
Cuts at: 1865 1865
Size: 3953
BsrFI r'CCGG_y
Cuts at: 170 1951 2132 2341 170
Size: 1781 181 209 1782
Fragments arranged by size:
1782 1781 209 181
BssSI C'ACGA_G
Cuts at: 2224 2582 2224
Size: 358 3595
BstAPI GCAn_nnn'nTGC
Cuts at: 1330 1330
Size: 3953
BstBI TT'CG_AA
Cuts at: 2314 2314
Size: 3953
BstXI CCAn_nnnn'nTGG
Cuts at: 815 815
Size: 3953
BstYI r'GATC_y
Cuts at: 1 486 547 729 978 1445 1468 1803 2049
Size: 485 61 182 249 467 23 335 246
Cuts at: 2049 3061 3147 3159 3505 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 485 467 449 346 335 249 246
182 86 61 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1903 2084 2247 1903
Size: 181 163 3609
Fragments arranged by size:
3609 181 163
BtsI GCAGTG_nn'
Cuts at: 1196 1196
Size: 3953
DraI TTT'AAA
Cuts at: 335 952 335
Size: 617 3336
DrdI GACnn_nn'nnGTC
Cuts at: 1659 2517 1659
Size: 858 3095
EaeI y'GGCC_r
Cuts at: 1538 1712 2103 2130 3475 1538
Size: 174 391 27 1345 2016
Fragments arranged by size:
2016 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1538 1538
Size: 3953
EarI CTCTTCn'nnn_
Cuts at: 1976 2186 1976
Size: 210 3743
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2161 2471 2617 3763 2161
Size: 310 146 1146 2351
Fragments arranged by size:
2351 1146 310 146
EcoICRI GAG'CTC
Cuts at: 965 965
Size: 3953
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 1778 2210 2957 3225 349
Size: 1429 432 747 268 1077
Fragments arranged by size:
1429 1077 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1092 1092
Size: 3953
EcoRI G'AATT_C
Cuts at: 957 957
Size: 3953
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 722 1972 722
Size: 1250 2703
FspI TGC'GCA
Cuts at: 1734 1734
Size: 3953
HaeII r_GCGC'y
Cuts at: 1387 1635 2657 3636 1387
Size: 248 1022 979 1704
Fragments arranged by size:
1704 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 925 957 1795 1825 1827 1857 925
Size: 32 838 30 2 30 3021
Fragments arranged by size:
3021 838 32 30 30 2
HincII GTy'rAC
Cuts at: 992 992
Size: 3953
HindIII A'AGCT_T
Cuts at: 704 704
Size: 3953
KasI G'GCGC_C
Cuts at: 1383 1631 1383
Size: 248 3705
KpnI G_GTAC'C
Cuts at: 973 973
Size: 3953
MfeI C'AATT_G
Cuts at: 809 809
Size: 3953
MluI A'CGCG_T
Cuts at: 3386 3386
Size: 3953
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2624 2808 2624
Size: 184 3769
MscI TGG'CCA
Cuts at: 1714 1714
Size: 3953
MslI CAynn'nnrTG
Cuts at: 1942 2069 1942
Size: 127 3826
MspA1I CmG'CkG
Cuts at: 573 1064 1378 1738 2751 2996 573
Size: 491 314 360 1013 245 1530
Fragments arranged by size:
1530 1013 491 360 314 245
NaeI GCC'GGC
Cuts at: 172 2134 172
Size: 1962 1991
NarI GG'CG_CC
Cuts at: 1384 1632 1384
Size: 248 3705
NgoMIV G'CCGG_C
Cuts at: 170 2132 170
Size: 1962 1991
NspI r_CATG'y
Cuts at: 234 1006 1506 2413 234
Size: 772 500 907 1774
Fragments arranged by size:
1774 907 772 500
PciI A'CATG_T
Cuts at: 2409 2409
Size: 3953
PmeI GTTT'AAAC
Cuts at: 952 952
Size: 3953
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3092 3124 3092
Size: 32 3921
PstI C_TGCA'G
Cuts at: 1000 1685 1000
Size: 685 3268
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3953
PvuII CAG'CTG
Cuts at: 1378 1738 1378
Size: 360 3593
RsrII CG'GwC_CG
Cuts at: 2148 2148
Size: 3953
SacI G_AGCT'C
Cuts at: 967 967
Size: 3953
SalI G'TCGA_C
Cuts at: 990 990
Size: 3953
SapI GCTCTTCn'nnn_
Cuts at: 1976 2186 1976
Size: 210 3743
SbfI CC_TGCA'GG
Cuts at: 1000 1000
Size: 3953
ScaI AGT'ACT
Cuts at: 915 3470 915
Size: 2555 1398
SfcI C'TryA_G
Cuts at: 33 352 996 1681 2674 2865 33
Size: 319 644 685 993 191 1121
Fragments arranged by size:
1121 993 685 644 319 191
SfoI GGC'GCC
Cuts at: 1385 1633 1385
Size: 248 3705
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3953
SmaI CCC'GGG
Cuts at: 975 975
Size: 3953
SmlI C'TyrA_G
Cuts at: 202 1108 2023 2515 2777 202
Size: 906 915 492 262 1378
Fragments arranged by size:
1378 915 906 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3953
SphI G_CATG'C
Cuts at: 1006 1506 1006
Size: 500 3453
StyI C'CwwG_G
Cuts at: 683 1087 1441 683
Size: 404 354 3195
Fragments arranged by size:
3195 404 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1536 1669 2128 2337 1536
Size: 133 459 209 3152
Fragments arranged by size:
3152 459 209 133
TatI w'GTAC_w
Cuts at: 134 913 3468 134
Size: 779 2555 619
Fragments arranged by size:
2555 779 619
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 464 496 3470 3502 464
Size: 32 2974 32 915
Fragments arranged by size:
2974 915 32 32
Tth111I GACn'n_nGTC
Cuts at: 1750 1750
Size: 3953
XbaI T'CTAG_A
Cuts at: 984 984
Size: 3953
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 769 860 1448 769
Size: 91 588 3274
Fragments arranged by size:
3274 588 91
XmaI C'CCGG_G
Cuts at: 973 973
Size: 3953
XmnI GAAnn'nnTTC
Cuts at: 818 818
Size: 3953
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI