Restriction map for
pF1KB8902
(Circular) (Six-base) MAPSORT of: pF1KB8902.seq Check: 1697 from: 1 to: 3815
pF1KB8902 3815 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 853 853
Size: 3815
Acc65I G'GTAC_C
Cuts at: 831 831
Size: 3815
AclI AA'CG_TT
Cuts at: 3655 3655
Size: 3815
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 419 1640 2072 2819 3087 419
Size: 1221 432 747 268 1147
Fragments arranged by size:
1221 1147 747 432 268
AfeI AGC'GCT
Cuts at: 484 484
Size: 3815
AflIII A'CryG_T
Cuts at: 1167 2271 3248 1167
Size: 1104 977 1734
Fragments arranged by size:
1734 1104 977
AgeI A'CCGG_T
Cuts at: 2203 2203
Size: 3815
AleI CACnn'nnGTG
Cuts at: 511 511
Size: 3815
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 517 549 517
Size: 32 3783
AlwNI CAG_nnn'CTG
Cuts at: 1120 2687 1120
Size: 1567 2248
ApaI G_GGCC'C
Cuts at: 347 573 347
Size: 226 3589
ApaLI G'TGCA_C
Cuts at: 2585 2585
Size: 3815
ApoI r'AATT_y
Cuts at: 819 3168 3424 819
Size: 2349 256 1210
Fragments arranged by size:
2349 1210 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3815
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3815
AvaI C'yCGr_G
Cuts at: 133 488 725 835 133
Size: 355 237 110 3113
Fragments arranged by size:
3113 355 237 110
BamHI G'GATC_C
Cuts at: 840 840
Size: 3815
BanI G'GyrC_C
Cuts at: 439 831 1245 1493 1528 439
Size: 392 414 248 35 2726
Fragments arranged by size:
2726 414 392 248 35
BanII G_rGCy'C
Cuts at: 347 573 799 829 1859 347
Size: 226 226 30 1030 2303
Fragments arranged by size:
2303 1030 226 226 30
BbeI G_GCGC'C
Cuts at: 443 1249 1497 443
Size: 806 248 2761
Fragments arranged by size:
2761 806 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3781
BciVI GTATCCnnnnn_n'
Cuts at: 1713 2474 3815 1713
Size: 761 1341 1713
Fragments arranged by size:
1713 1341 761
BclI T'GATC_A
Cuts at: 1335 1335
Size: 3815
BglI GCCn_nnn'nGGC
Cuts at: 396 577 396
Size: 181 3634
BglII A'GATC_T
Cuts at: 1330 3367 1330
Size: 2037 1778
BlpI GC'TnA_GC
Cuts at: 334 927 334
Size: 593 3222
Bme1580I G_kGCm'C
Cuts at: 347 573 1440 1533 2589 347
Size: 226 867 93 1056 1573
Fragments arranged by size:
1573 1056 867 226 93
BmgBI CAC'GTC
Cuts at: 188 188
Size: 3815
BmrI ACTGGGnnnn_n'
Cuts at: 1027 1108 1443 3587 1027
Size: 81 335 2144 1255
Fragments arranged by size:
2144 1255 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 705 738 2182 705
Size: 33 1444 2338
Fragments arranged by size:
2338 1444 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 813 813
Size: 3815
Bpu10I CC'TnA_GC
Cuts at: 112 467 112
Size: 355 3460
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 991 1870 2362 2660 574
Size: 417 879 492 298 1729
Fragments arranged by size:
1729 879 492 417 298
BsaI GGTCTCn'nnnn_
Cuts at: 376 595 744 3519 376
Size: 219 149 2775 672
Fragments arranged by size:
2775 672 219 149
BsaAI yAC'GTr
Cuts at: 1168 1168
Size: 3815
BsaBI GATnn'nnATC
Cuts at: 6 1329 3366 6
Size: 1323 2037 455
Fragments arranged by size:
2037 1323 455
BsaHI Gr'CG_yC
Cuts at: 440 1246 1494 2196 3692 440
Size: 806 248 702 1496 563
Fragments arranged by size:
1496 806 702 563 248
BsaWI w'CCGG_w
Cuts at: 120 1004 1525 2203 2477 2624 3035 120
Size: 884 521 678 274 147 411 900
Fragments arranged by size:
900 884 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 517 547 517
Size: 30 3785
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 376 651 681 727 376
Size: 275 30 46 3464
Fragments arranged by size:
3464 275 46 30
BseYI C'CCAG_C
Cuts at: 669 798 1240 1998 2575 3071 669
Size: 129 442 758 577 496 1413
Fragments arranged by size:
1413 758 577 496 442 129
BsiEI CG_ry'CG
Cuts at: 104 240 1357 1403 2611 104
Size: 136 1117 46 1208 1308
Fragments arranged by size:
1308 1208 1117 136 46
BsiHKAI G_wGCw'C
Cuts at: 340 829 1607 1797 2589 340
Size: 489 778 190 792 1566
Fragments arranged by size:
1566 792 778 489 190
BsmBI CGTCTCn'nnnn_
Cuts at: 364 364
Size: 3815
Bsp1286I G_dGCh'C
Cuts at: 340 347 573 799 829 1440 1533 1607 1797
Size: 7 226 226 30 611 93 74 190
Cuts at: 1797 1859 2589 340
Size: 62 730 1566
Fragments arranged by size:
1566 730 611 226 226 190 93 74
62 30 7
BspEI T'CCGG_A
Cuts at: 120 3035 120
Size: 2915 900
BspHI T'CATG_A
Cuts at: 2991 2991
Size: 3815
BspMI ACCTGCnnnn'nnnn_
Cuts at: 865 1079 1381 1762 865
Size: 214 302 381 2918
Fragments arranged by size:
2918 381 302 214
BsrBI CCG'CTC
Cuts at: 50 183 2107 2161 3645 3810 50
Size: 133 1924 54 1484 165 55
Fragments arranged by size:
1924 1484 165 133 55 54
BsrDI GCAATG_nn'
Cuts at: 1727 1727
Size: 3815
BsrFI r'CCGG_y
Cuts at: 699 1813 1994 2203 699
Size: 1114 181 209 2311
Fragments arranged by size:
2311 1114 209 181
BssHII G'CGCG_C
Cuts at: 221 221
Size: 3815
BssSI C'ACGA_G
Cuts at: 243 2086 2444 243
Size: 1843 358 1614
Fragments arranged by size:
1843 1614 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1192 1192
Size: 3815
BstBI TT'CG_AA
Cuts at: 2176 2176
Size: 3815
BstXI CCAn_nnnn'nTGG
Cuts at: 806 806
Size: 3815
BstYI r'GATC_y
Cuts at: 1 840 1307 1330 1665 1911 2923 3009 3021
Size: 839 467 23 335 246 1012 86 12
Cuts at: 3021 3367 1
Size: 346 449
Fragments arranged by size:
1012 839 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 127 127
Size: 3815
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1765 1946 2109 1765
Size: 181 163 3471
Fragments arranged by size:
3471 181 163
BtsI GCAGTG_nn'
Cuts at: 1058 1058
Size: 3815
DraI TTT'AAA
Cuts at: 814 814
Size: 3815
DrdI GACnn_nn'nnGTC
Cuts at: 1521 2379 1521
Size: 858 2957
EaeI y'GGCC_r
Cuts at: 237 578 1400 1574 1965 1992 3337 237
Size: 341 822 174 391 27 1345 715
Fragments arranged by size:
1345 822 715 391 341 174 27
EagI C'GGCC_G
Cuts at: 237 1400 237
Size: 1163 2652
EarI CTCTTCn'nnn_
Cuts at: 1838 2048 1838
Size: 210 3605
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2023 2333 2479 3625 2023
Size: 310 146 1146 2213
Fragments arranged by size:
2213 1146 310 146
EcoICRI GAG'CTC
Cuts at: 827 827
Size: 3815
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 419 813 1640 2072 2819 3087 419
Size: 394 827 432 747 268 1147
Fragments arranged by size:
1147 827 747 432 394 268
EcoNI CCTnn'n_nnAGG
Cuts at: 199 709 199
Size: 510 3305
EcoO109I rG'GnC_Cy
Cuts at: 344 954 344
Size: 610 3205
EcoRI G'AATT_C
Cuts at: 819 819
Size: 3815
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1834 1834
Size: 3815
FspI TGC'GCA
Cuts at: 1596 1596
Size: 3815
HaeII r_GCGC'y
Cuts at: 443 486 1249 1497 2519 3498 443
Size: 43 763 248 1022 979 760
Fragments arranged by size:
1022 979 763 760 248 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1657 1687 1689 1719 1657
Size: 30 2 30 3753
Fragments arranged by size:
3753 30 30 2
HincII GTy'rAC
Cuts at: 854 854
Size: 3815
KasI G'GCGC_C
Cuts at: 439 1245 1493 439
Size: 806 248 2761
Fragments arranged by size:
2761 806 248
KpnI G_GTAC'C
Cuts at: 835 835
Size: 3815
MluI A'CGCG_T
Cuts at: 3248 3248
Size: 3815
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 398 503 2486 2670 398
Size: 105 1983 184 1543
Fragments arranged by size:
1983 1543 184 105
MscI TGG'CCA
Cuts at: 580 1576 580
Size: 996 2819
MslI CAynn'nnrTG
Cuts at: 511 1804 1931 511
Size: 1293 127 2395
Fragments arranged by size:
2395 1293 127
MspA1I CmG'CkG
Cuts at: 129 228 333 791 926 1240 1600 2613 2858
Size: 99 105 458 135 314 360 1013 245
Cuts at: 2858 129
Size: 1086
Fragments arranged by size:
1086 1013 458 360 314 245 135 105
99
NaeI GCC'GGC
Cuts at: 701 1996 701
Size: 1295 2520
NarI GG'CG_CC
Cuts at: 440 1246 1494 440
Size: 806 248 2761
Fragments arranged by size:
2761 806 248
NgoMIV G'CCGG_C
Cuts at: 699 1994 699
Size: 1295 2520
NotI GC'GGCC_GC
Cuts at: 237 237
Size: 3815
NspI r_CATG'y
Cuts at: 868 1368 2275 868
Size: 500 907 2408
Fragments arranged by size:
2408 907 500
PciI A'CATG_T
Cuts at: 2271 2271
Size: 3815
PfoI T'CCnGG_A
Cuts at: 200 200
Size: 3815
PmeI GTTT'AAAC
Cuts at: 814 814
Size: 3815
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 517 549 2954 2986 517
Size: 32 2405 32 1346
Fragments arranged by size:
2405 1346 32 32
PspOMI G'GGCC_C
Cuts at: 343 569 343
Size: 226 3589
PstI C_TGCA'G
Cuts at: 862 1547 862
Size: 685 3130
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3815
PvuII CAG'CTG
Cuts at: 333 791 1240 1600 333
Size: 458 449 360 2548
Fragments arranged by size:
2548 458 449 360
RsrII CG'GwC_CG
Cuts at: 2010 2010
Size: 3815
SacI G_AGCT'C
Cuts at: 829 829
Size: 3815
SacII CC_GC'GG
Cuts at: 130 130
Size: 3815
SalI G'TCGA_C
Cuts at: 852 852
Size: 3815
SapI GCTCTTCn'nnn_
Cuts at: 1838 2048 1838
Size: 210 3605
SbfI CC_TGCA'GG
Cuts at: 862 862
Size: 3815
ScaI AGT'ACT
Cuts at: 3332 3332
Size: 3815
SfcI C'TryA_G
Cuts at: 33 858 1543 2536 2727 33
Size: 825 685 993 191 1121
Fragments arranged by size:
1121 993 825 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 577 577
Size: 3815
SfoI GGC'GCC
Cuts at: 441 1247 1495 441
Size: 806 248 2761
Fragments arranged by size:
2761 806 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3815
SmaI CCC'GGG
Cuts at: 135 727 837 135
Size: 592 110 3113
Fragments arranged by size:
3113 592 110
SmlI C'TyrA_G
Cuts at: 589 970 1885 2377 2639 589
Size: 381 915 492 262 1765
Fragments arranged by size:
1765 915 492 381 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3815
SphI G_CATG'C
Cuts at: 868 1368 868
Size: 500 3315
StuI AGG'CCT
Cuts at: 718 718
Size: 3815
StyI C'CwwG_G
Cuts at: 448 949 1303 448
Size: 501 354 2960
Fragments arranged by size:
2960 501 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 400 1398 1531 1990 2199 400
Size: 998 133 459 209 2016
Fragments arranged by size:
2016 998 459 209 133
TatI w'GTAC_w
Cuts at: 776 3330 776
Size: 2554 1261
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3332 3364 3332
Size: 32 3783
Tth111I GACn'n_nGTC
Cuts at: 171 1612 171
Size: 1441 2374
XbaI T'CTAG_A
Cuts at: 846 846
Size: 3815
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1310 1310
Size: 3815
XmaI C'CCGG_G
Cuts at: 133 725 835 133
Size: 592 110 3113
Fragments arranged by size:
3113 592 110
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NotI NspI
PciI PfoI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AscI AvrII BaeI
BbsI BbvCI BfrBI BmtI BsgI BsiWI BsmI BsrGI
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NruI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspXI PsrI SanDI SexAI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI