Restriction map for
pF1KB8896
(Circular) (Six-base) MAPSORT of: pF1KB8896.seq Check: 2742 from: 1 to: 3782
pF1KB8896 3782 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AccI GT'mk_AC
Cuts at: 820 820
Size: 3782
Acc65I G'GTAC_C
Cuts at: 688 798 688
Size: 110 3672
AclI AA'CG_TT
Cuts at: 3622 3622
Size: 3782
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1607 2039 2786 3054 1607
Size: 432 747 268 2335
Fragments arranged by size:
2335 747 432 268
AflIII A'CryG_T
Cuts at: 638 1134 2238 3215 638
Size: 496 1104 977 1205
Fragments arranged by size:
1205 1104 977 496
AgeI A'CCGG_T
Cuts at: 2170 2170
Size: 3782
AleI CACnn'nnGTG
Cuts at: 446 446
Size: 3782
AlwNI CAG_nnn'CTG
Cuts at: 726 1087 2654 726
Size: 361 1567 1854
Fragments arranged by size:
1854 1567 361
ApaLI G'TGCA_C
Cuts at: 439 2552 439
Size: 2113 1669
ApoI r'AATT_y
Cuts at: 481 786 3135 3391 481
Size: 305 2349 256 872
Fragments arranged by size:
2349 872 305 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3782
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3782
AvaI C'yCGr_G
Cuts at: 802 802
Size: 3782
BamHI G'GATC_C
Cuts at: 807 807
Size: 3782
BanI G'GyrC_C
Cuts at: 688 798 1212 1460 1495 688
Size: 110 414 248 35 2975
Fragments arranged by size:
2975 414 248 110 35
BanII G_rGCy'C
Cuts at: 796 1826 796
Size: 1030 2752
BbeI G_GCGC'C
Cuts at: 1216 1464 1216
Size: 248 3534
BbsI GAAGACnn'nnnn_
Cuts at: 399 399
Size: 3782
BciVI GTATCCnnnnn_n'
Cuts at: 1680 2441 3782 1680
Size: 761 1341 1680
Fragments arranged by size:
1680 1341 761
BclI T'GATC_A
Cuts at: 216 230 1302 216
Size: 14 1072 2696
Fragments arranged by size:
2696 1072 14
BglII A'GATC_T
Cuts at: 1297 3334 1297
Size: 2037 1745
BlpI GC'TnA_GC
Cuts at: 545 894 545
Size: 349 3433
Bme1580I G_kGCm'C
Cuts at: 443 1407 1500 2556 443
Size: 964 93 1056 1669
Fragments arranged by size:
1669 1056 964 93
BmrI ACTGGGnnnn_n'
Cuts at: 994 1075 1410 3554 994
Size: 81 335 2144 1222
Fragments arranged by size:
2144 1222 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2149 2149
Size: 3782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 419 262
Size: 157 3625
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 958 1837 2329 2627 958
Size: 879 492 298 2113
Fragments arranged by size:
2113 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3486 3486
Size: 3782
BsaAI yAC'GTr
Cuts at: 1135 1135
Size: 3782
BsaBI GATnn'nnATC
Cuts at: 6 1296 3333 6
Size: 1290 2037 455
Fragments arranged by size:
2037 1290 455
BsaHI Gr'CG_yC
Cuts at: 1213 1461 2163 3659 1213
Size: 248 702 1496 1336
Fragments arranged by size:
1496 1336 702 248
BsaWI w'CCGG_w
Cuts at: 971 1492 2170 2444 2591 3002 971
Size: 521 678 274 147 411 1751
Fragments arranged by size:
1751 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 455 1207 1965 2542 3038 455
Size: 752 758 577 496 1199
Fragments arranged by size:
1199 758 752 577 496
BsiEI CG_ry'CG
Cuts at: 104 1324 1370 2578 104
Size: 1220 46 1208 1308
Fragments arranged by size:
1308 1220 1208 46
BsiHKAI G_wGCw'C
Cuts at: 443 796 1574 1764 2556 443
Size: 353 778 190 792 1669
Fragments arranged by size:
1669 792 778 353 190
Bsp1286I G_dGCh'C
Cuts at: 443 796 1407 1500 1574 1764 1826 2556 443
Size: 353 611 93 74 190 62 730 1669
Fragments arranged by size:
1669 730 611 353 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3002 3002
Size: 3782
BspHI T'CATG_A
Cuts at: 2958 2958
Size: 3782
BspMI ACCTGCnnnn'nnnn_
Cuts at: 832 1046 1348 1729 832
Size: 214 302 381 2885
Fragments arranged by size:
2885 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2074 2128 3612 3777 50
Size: 2024 54 1484 165 55
Fragments arranged by size:
2024 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1694 1694
Size: 3782
BsrFI r'CCGG_y
Cuts at: 1780 1961 2170 1780
Size: 181 209 3392
Fragments arranged by size:
3392 209 181
BsrGI T'GTAC_A
Cuts at: 635 635
Size: 3782
BssSI C'ACGA_G
Cuts at: 296 2053 2411 296
Size: 1757 358 1667
Fragments arranged by size:
1757 1667 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1159 1159
Size: 3782
BstBI TT'CG_AA
Cuts at: 2143 2143
Size: 3782
BstXI CCAn_nnnn'nTGG
Cuts at: 150 711 150
Size: 561 3221
BstYI r'GATC_y
Cuts at: 1 128 807 1274 1297 1632 1878 2890 2976
Size: 127 679 467 23 335 246 1012 86
Cuts at: 2976 2988 3334 1
Size: 12 346 449
Fragments arranged by size:
1012 679 467 449 346 335 246 127
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1732 1913 2076 1732
Size: 181 163 3438
Fragments arranged by size:
3438 181 163
BtsI GCAGTG_nn'
Cuts at: 1025 1025
Size: 3782
DraI TTT'AAA
Cuts at: 781 781
Size: 3782
DrdI GACnn_nn'nnGTC
Cuts at: 1488 2346 1488
Size: 858 2924
EaeI y'GGCC_r
Cuts at: 1367 1541 1932 1959 3304 1367
Size: 174 391 27 1345 1845
Fragments arranged by size:
1845 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1367 1367
Size: 3782
EarI CTCTTCn'nnn_
Cuts at: 316 1805 2015 316
Size: 1489 210 2083
Fragments arranged by size:
2083 1489 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1990 2300 2446 3592 1990
Size: 310 146 1146 2180
Fragments arranged by size:
2180 1146 310 146
EcoICRI GAG'CTC
Cuts at: 794 794
Size: 3782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 419 1607 2039 2786 3054 262
Size: 157 1188 432 747 268 990
Fragments arranged by size:
1188 990 747 432 268 157
EcoNI CCTnn'n_nnAGG
Cuts at: 565 565
Size: 3782
EcoO109I rG'GnC_Cy
Cuts at: 921 921
Size: 3782
EcoRI G'AATT_C
Cuts at: 786 786
Size: 3782
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1801 1801
Size: 3782
FspI TGC'GCA
Cuts at: 1563 1563
Size: 3782
HaeII r_GCGC'y
Cuts at: 1216 1464 2486 3465 1216
Size: 248 1022 979 1533
Fragments arranged by size:
1533 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 451 483 734 737 766 769 1624 1654 1656
Size: 32 251 3 29 3 855 30 2
Cuts at: 1656 1686 451
Size: 30 2547
Fragments arranged by size:
2547 855 251 32 30 30 29 3
3 2
HincII GTy'rAC
Cuts at: 821 821
Size: 3782
KasI G'GCGC_C
Cuts at: 1212 1460 1212
Size: 248 3534
KpnI G_GTAC'C
Cuts at: 692 802 692
Size: 110 3672
MluI A'CGCG_T
Cuts at: 3215 3215
Size: 3782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 348 2453 2637 348
Size: 2105 184 1493
Fragments arranged by size:
2105 1493 184
MscI TGG'CCA
Cuts at: 1543 1543
Size: 3782
MslI CAynn'nnrTG
Cuts at: 446 448 1771 1898 446
Size: 2 1323 127 2330
Fragments arranged by size:
2330 1323 127 2
MspA1I CmG'CkG
Cuts at: 120 240 893 1207 1567 2580 2825 120
Size: 120 653 314 360 1013 245 1077
Fragments arranged by size:
1077 1013 653 360 314 245 120
NaeI GCC'GGC
Cuts at: 1963 1963
Size: 3782
NarI GG'CG_CC
Cuts at: 1213 1461 1213
Size: 248 3534
NgoMIV G'CCGG_C
Cuts at: 1961 1961
Size: 3782
NspI r_CATG'y
Cuts at: 642 835 1335 2242 642
Size: 193 500 907 2182
Fragments arranged by size:
2182 907 500 193
PciI A'CATG_T
Cuts at: 638 2238 638
Size: 1600 2182
PfoI T'CCnGG_A
Cuts at: 149 710 149
Size: 561 3221
PmeI GTTT'AAAC
Cuts at: 781 781
Size: 3782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2921 2953 2921
Size: 32 3750
PsiI TTA'TAA
Cuts at: 356 356
Size: 3782
PstI C_TGCA'G
Cuts at: 829 1514 829
Size: 685 3097
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3782
PvuII CAG'CTG
Cuts at: 1207 1567 1207
Size: 360 3422
RsrII CG'GwC_CG
Cuts at: 1977 1977
Size: 3782
SacI G_AGCT'C
Cuts at: 796 796
Size: 3782
SalI G'TCGA_C
Cuts at: 819 819
Size: 3782
SapI GCTCTTCn'nnn_
Cuts at: 1805 2015 1805
Size: 210 3572
SbfI CC_TGCA'GG
Cuts at: 829 829
Size: 3782
ScaI AGT'ACT
Cuts at: 3299 3299
Size: 3782
SfcI C'TryA_G
Cuts at: 33 253 282 303 825 1510 2503 2694 33
Size: 220 29 21 522 685 993 191 1121
Fragments arranged by size:
1121 993 685 522 220 191 29 21
SfoI GGC'GCC
Cuts at: 1214 1462 1214
Size: 248 3534
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3782
SmaI CCC'GGG
Cuts at: 804 804
Size: 3782
SmlI C'TyrA_G
Cuts at: 937 1852 2344 2606 937
Size: 915 492 262 2113
Fragments arranged by size:
2113 915 492 262
SpeI A'CTAG_T
Cuts at: 67 348 67
Size: 281 3501
SphI G_CATG'C
Cuts at: 835 1335 835
Size: 500 3282
StyI C'CwwG_G
Cuts at: 327 764 916 1270 327
Size: 437 152 354 2839
Fragments arranged by size:
2839 437 354 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1365 1498 1957 2166 1365
Size: 133 459 209 2981
Fragments arranged by size:
2981 459 209 133
TatI w'GTAC_w
Cuts at: 635 3297 635
Size: 2662 1120
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3299 3331 3299
Size: 32 3750
Tth111I GACn'n_nGTC
Cuts at: 1579 1579
Size: 3782
XbaI T'CTAG_A
Cuts at: 813 813
Size: 3782
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 558 1277 558
Size: 719 3063
XmaI C'CCGG_G
Cuts at: 802 802
Size: 3782
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PfoI PmeI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI