Restriction map for
pF1KB8889
(Circular) (Six-base) MAPSORT of: pF1KB8889.seq Check: 5783 from: 1 to: 3725
pF1KB8889 3725 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AatII G_ACGT'C
Cuts at: 509 509
Size: 3725
AccI GT'mk_AC
Cuts at: 763 763
Size: 3725
Acc65I G'GTAC_C
Cuts at: 741 741
Size: 3725
AclI AA'CG_TT
Cuts at: 3565 3565
Size: 3725
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 600 1550 1982 2729 2997 600
Size: 950 432 747 268 1328
Fragments arranged by size:
1328 950 747 432 268
AflIII A'CryG_T
Cuts at: 1077 2181 3158 1077
Size: 1104 977 1644
Fragments arranged by size:
1644 1104 977
AgeI A'CCGG_T
Cuts at: 287 2113 287
Size: 1826 1899
AlwNI CAG_nnn'CTG
Cuts at: 528 1030 2597 528
Size: 502 1567 1656
Fragments arranged by size:
1656 1567 502
ApaLI G'TGCA_C
Cuts at: 2495 2495
Size: 3725
ApoI r'AATT_y
Cuts at: 729 3078 3334 729
Size: 2349 256 1120
Fragments arranged by size:
2349 1120 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3725
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3725
AvaI C'yCGr_G
Cuts at: 200 217 238 319 512 745 200
Size: 17 21 81 193 233 3180
Fragments arranged by size:
3180 233 193 81 21 17
BamHI G'GATC_C
Cuts at: 750 750
Size: 3725
BanI G'GyrC_C
Cuts at: 211 225 741 1155 1403 1438 211
Size: 14 516 414 248 35 2498
Fragments arranged by size:
2498 516 414 248 35 14
BanII G_rGCy'C
Cuts at: 547 739 1769 547
Size: 192 1030 2503
Fragments arranged by size:
2503 1030 192
BbeI G_GCGC'C
Cuts at: 229 1159 1407 229
Size: 930 248 2547
Fragments arranged by size:
2547 930 248
BciVI GTATCCnnnnn_n'
Cuts at: 1623 2384 3725 1623
Size: 761 1341 1623
Fragments arranged by size:
1623 1341 761
BclI T'GATC_A
Cuts at: 1245 1245
Size: 3725
BglII A'GATC_T
Cuts at: 1240 3277 1240
Size: 2037 1688
BlpI GC'TnA_GC
Cuts at: 541 837 541
Size: 296 3429
Bme1580I G_kGCm'C
Cuts at: 1350 1443 2499 1350
Size: 93 1056 2576
Fragments arranged by size:
2576 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 937 1018 1353 3497 937
Size: 81 335 2144 1165
Fragments arranged by size:
2144 1165 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2092 2092
Size: 3725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 451
Size: 3725
Bpu10I CC'TnA_GC
Cuts at: 584 584
Size: 3725
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 1780 2272 2570 901
Size: 879 492 298 2056
Fragments arranged by size:
2056 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3429 3429
Size: 3725
BsaAI yAC'GTr
Cuts at: 1078 1078
Size: 3725
BsaBI GATnn'nnATC
Cuts at: 6 1239 3276 6
Size: 1233 2037 455
Fragments arranged by size:
2037 1233 455
BsaHI Gr'CG_yC
Cuts at: 226 506 1156 1404 2106 3602 226
Size: 280 650 248 702 1496 349
Fragments arranged by size:
1496 702 650 349 280 248
BsaWI w'CCGG_w
Cuts at: 287 914 1435 2113 2387 2534 2945 287
Size: 627 521 678 274 147 411 1067
Fragments arranged by size:
1067 678 627 521 411 274 147
BseYI C'CCAG_C
Cuts at: 593 1150 1908 2485 2981 593
Size: 557 758 577 496 1337
Fragments arranged by size:
1337 758 577 557 496
BsiEI CG_ry'CG
Cuts at: 104 266 1267 1313 2521 104
Size: 162 1001 46 1208 1308
Fragments arranged by size:
1308 1208 1001 162 46
BsiHKAI G_wGCw'C
Cuts at: 365 547 739 1517 1707 2499 365
Size: 182 192 778 190 792 1591
Fragments arranged by size:
1591 792 778 192 190 182
Bsp1286I G_dGCh'C
Cuts at: 365 547 739 1350 1443 1517 1707 1769 2499
Size: 182 192 611 93 74 190 62 730
Cuts at: 2499 365
Size: 1591
Fragments arranged by size:
1591 730 611 192 190 182 93 74
62
BspEI T'CCGG_A
Cuts at: 2945 2945
Size: 3725
BspHI T'CATG_A
Cuts at: 2901 2901
Size: 3725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 775 989 1291 1672 775
Size: 214 302 381 2828
Fragments arranged by size:
2828 381 302 214
BsrBI CCG'CTC
Cuts at: 50 168 2017 2071 3555 3720 50
Size: 118 1849 54 1484 165 55
Fragments arranged by size:
1849 1484 165 118 55 54
BsrDI GCAATG_nn'
Cuts at: 1637 1637
Size: 3725
BsrFI r'CCGG_y
Cuts at: 222 287 1723 1904 2113 222
Size: 65 1436 181 209 1834
Fragments arranged by size:
1834 1436 209 181 65
BssSI C'ACGA_G
Cuts at: 406 1996 2354 406
Size: 1590 358 1777
Fragments arranged by size:
1777 1590 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1102 1102
Size: 3725
BstBI TT'CG_AA
Cuts at: 2086 2086
Size: 3725
BstEII G'GTnAC_C
Cuts at: 447 447
Size: 3725
BstYI r'GATC_y
Cuts at: 1 750 1217 1240 1575 1821 2833 2919 2931
Size: 749 467 23 335 246 1012 86 12
Cuts at: 2931 3277 1
Size: 346 449
Fragments arranged by size:
1012 749 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 137 260 481 107
Size: 30 123 221 3351
Fragments arranged by size:
3351 221 123 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1675 1856 2019 1675
Size: 181 163 3381
Fragments arranged by size:
3381 181 163
BtsI GCAGTG_nn'
Cuts at: 968 968
Size: 3725
DraI TTT'AAA
Cuts at: 724 724
Size: 3725
DrdI GACnn_nn'nnGTC
Cuts at: 1431 2289 1431
Size: 858 2867
EaeI y'GGCC_r
Cuts at: 140 176 263 1310 1484 1875 1902 3247 140
Size: 36 87 1047 174 391 27 1345 618
Fragments arranged by size:
1345 1047 618 391 174 87 36 27
EagI C'GGCC_G
Cuts at: 263 1310 263
Size: 1047 2678
EarI CTCTTCn'nnn_
Cuts at: 1748 1958 1748
Size: 210 3515
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 291 367 1933 2243 2389 3535 291
Size: 76 1566 310 146 1146 481
Fragments arranged by size:
1566 1146 481 310 146 76
EcoICRI GAG'CTC
Cuts at: 545 737 545
Size: 192 3533
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 600 1550 1982 2729 2997 451
Size: 149 950 432 747 268 1179
Fragments arranged by size:
1179 950 747 432 268 149
EcoO109I rG'GnC_Cy
Cuts at: 620 864 620
Size: 244 3481
EcoRI G'AATT_C
Cuts at: 729 729
Size: 3725
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 489 1744 489
Size: 1255 2470
FspI TGC'GCA
Cuts at: 1506 1506
Size: 3725
HaeII r_GCGC'y
Cuts at: 229 346 528 1159 1407 2429 3408 229
Size: 117 182 631 248 1022 979 546
Fragments arranged by size:
1022 979 631 546 248 182 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1567 1597 1599 1629 1567
Size: 30 2 30 3663
Fragments arranged by size:
3663 30 30 2
HincII GTy'rAC
Cuts at: 114 683 764 114
Size: 569 81 3075
Fragments arranged by size:
3075 569 81
HpaI GTT'AAC
Cuts at: 683 683
Size: 3725
KasI G'GCGC_C
Cuts at: 225 1155 1403 225
Size: 930 248 2547
Fragments arranged by size:
2547 930 248
KpnI G_GTAC'C
Cuts at: 745 745
Size: 3725
MluI A'CGCG_T
Cuts at: 3158 3158
Size: 3725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 392 2396 2580 392
Size: 2004 184 1537
Fragments arranged by size:
2004 1537 184
MscI TGG'CCA
Cuts at: 1486 1486
Size: 3725
MslI CAynn'nnrTG
Cuts at: 136 1714 1841 136
Size: 1578 127 2020
Fragments arranged by size:
2020 1578 127
MspA1I CmG'CkG
Cuts at: 150 262 444 483 654 836 1150 1510 2523
Size: 112 182 39 171 182 314 360 1013
Cuts at: 2523 2768 150
Size: 245 1107
Fragments arranged by size:
1107 1013 360 314 245 182 182 171
112 39
NaeI GCC'GGC
Cuts at: 224 1906 224
Size: 1682 2043
NarI GG'CG_CC
Cuts at: 226 1156 1404 226
Size: 930 248 2547
Fragments arranged by size:
2547 930 248
NcoI C'CATG_G
Cuts at: 107 137 107
Size: 30 3695
NgoMIV G'CCGG_C
Cuts at: 222 1904 222
Size: 1682 2043
NotI GC'GGCC_GC
Cuts at: 263 263
Size: 3725
NspI r_CATG'y
Cuts at: 778 1278 2185 778
Size: 500 907 2318
Fragments arranged by size:
2318 907 500
PciI A'CATG_T
Cuts at: 2181 2181
Size: 3725
PflMI CCAn_nnn'nTGG
Cuts at: 528 528
Size: 3725
PmeI GTTT'AAAC
Cuts at: 724 724
Size: 3725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2864 2896 2864
Size: 32 3693
PpuMI rG'GwC_Cy
Cuts at: 620 620
Size: 3725
PstI C_TGCA'G
Cuts at: 581 772 1457 581
Size: 191 685 2849
Fragments arranged by size:
2849 685 191
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3725
PvuII CAG'CTG
Cuts at: 654 1150 1510 654
Size: 496 360 2869
Fragments arranged by size:
2869 496 360
RsrII CG'GwC_CG
Cuts at: 1920 1920
Size: 3725
SacI G_AGCT'C
Cuts at: 547 739 547
Size: 192 3533
SacII CC_GC'GG
Cuts at: 263 484 263
Size: 221 3504
SalI G'TCGA_C
Cuts at: 762 762
Size: 3725
SapI GCTCTTCn'nnn_
Cuts at: 1748 1958 1748
Size: 210 3515
SbfI CC_TGCA'GG
Cuts at: 772 772
Size: 3725
ScaI AGT'ACT
Cuts at: 3242 3242
Size: 3725
SfcI C'TryA_G
Cuts at: 33 577 768 1453 2446 2637 33
Size: 544 191 685 993 191 1121
Fragments arranged by size:
1121 993 685 544 191 191
SfoI GGC'GCC
Cuts at: 227 1157 1405 227
Size: 930 248 2547
Fragments arranged by size:
2547 930 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3725
SmaI CCC'GGG
Cuts at: 202 321 514 747 202
Size: 119 193 233 3180
Fragments arranged by size:
3180 233 193 119
SmlI C'TyrA_G
Cuts at: 880 1795 2287 2549 880
Size: 915 492 262 2056
Fragments arranged by size:
2056 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3725
SphI G_CATG'C
Cuts at: 778 1278 778
Size: 500 3225
StyI C'CwwG_G
Cuts at: 107 137 859 1213 107
Size: 30 722 354 2619
Fragments arranged by size:
2619 722 354 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1308 1441 1900 2109 1308
Size: 133 459 209 2924
Fragments arranged by size:
2924 459 209 133
TatI w'GTAC_w
Cuts at: 3240 3240
Size: 3725
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3242 3274 3242
Size: 32 3693
Tth111I GACn'n_nGTC
Cuts at: 1522 1522
Size: 3725
XbaI T'CTAG_A
Cuts at: 756 756
Size: 3725
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 638 1220 638
Size: 582 3143
XmaI C'CCGG_G
Cuts at: 200 319 512 745 200
Size: 119 193 233 3180
Fragments arranged by size:
3180 233 193 119
XmnI GAAnn'nnTTC
Cuts at: 328 328
Size: 3725
ZraI GAC'GTC
Cuts at: 507 507
Size: 3725
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PciI
PflMI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII MfeI NdeI NheI
NruI NsiI PacI PasI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI