Restriction map for
pF1KB8882
(Circular) (Six-base) MAPSORT of: pF1KB8882.seq Check: 9736 from: 1 to: 3659
pF1KB8882 3659 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AccI GT'mk_AC
Cuts at: 488 587 697 488
Size: 99 110 3450
Fragments arranged by size:
3450 110 99
Acc65I G'GTAC_C
Cuts at: 675 675
Size: 3659
AclI AA'CG_TT
Cuts at: 3499 3499
Size: 3659
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1484 1916 2663 2931 1484
Size: 432 747 268 2212
Fragments arranged by size:
2212 747 432 268
AfeI AGC'GCT
Cuts at: 190 190
Size: 3659
AflIII A'CryG_T
Cuts at: 1011 2115 3092 1011
Size: 1104 977 1578
Fragments arranged by size:
1578 1104 977
AgeI A'CCGG_T
Cuts at: 2047 2047
Size: 3659
AlwNI CAG_nnn'CTG
Cuts at: 426 964 2531 426
Size: 538 1567 1554
Fragments arranged by size:
1567 1554 538
ApaLI G'TGCA_C
Cuts at: 2429 2429
Size: 3659
ApoI r'AATT_y
Cuts at: 157 663 3012 3268 157
Size: 506 2349 256 548
Fragments arranged by size:
2349 548 506 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3659
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3659
AvaI C'yCGr_G
Cuts at: 168 260 449 548 679 168
Size: 92 189 99 131 3148
Fragments arranged by size:
3148 189 131 99 92
BamHI G'GATC_C
Cuts at: 684 684
Size: 3659
BanI G'GyrC_C
Cuts at: 413 675 1089 1337 1372 413
Size: 262 414 248 35 2700
Fragments arranged by size:
2700 414 262 248 35
BanII G_rGCy'C
Cuts at: 653 673 1703 653
Size: 20 1030 2609
Fragments arranged by size:
2609 1030 20
BbeI G_GCGC'C
Cuts at: 1093 1341 1093
Size: 248 3411
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 440 474 440
Size: 34 3625
BciVI GTATCCnnnnn_n'
Cuts at: 1557 2318 3659 1557
Size: 761 1341 1557
Fragments arranged by size:
1557 1341 761
BclI T'GATC_A
Cuts at: 1179 1179
Size: 3659
BglI GCCn_nnn'nGGC
Cuts at: 139 139
Size: 3659
BglII A'GATC_T
Cuts at: 1174 3211 1174
Size: 2037 1622
BlpI GC'TnA_GC
Cuts at: 771 771
Size: 3659
Bme1580I G_kGCm'C
Cuts at: 1284 1377 2433 1284
Size: 93 1056 2510
Fragments arranged by size:
2510 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 871 952 1287 3431 871
Size: 81 335 2144 1099
Fragments arranged by size:
2144 1099 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 220 529 2026 220
Size: 309 1497 1853
Fragments arranged by size:
1853 1497 309
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 418
Size: 3659
Bpu10I CC'TnA_GC
Cuts at: 307 307
Size: 3659
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 835 1714 2206 2504 835
Size: 879 492 298 1990
Fragments arranged by size:
1990 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3363 3363
Size: 3659
BsaAI yAC'GTr
Cuts at: 1012 1012
Size: 3659
BsaBI GATnn'nnATC
Cuts at: 6 1173 3210 6
Size: 1167 2037 455
Fragments arranged by size:
2037 1167 455
BsaHI Gr'CG_yC
Cuts at: 1090 1338 2040 3536 1090
Size: 248 702 1496 1213
Fragments arranged by size:
1496 1213 702 248
BsaWI w'CCGG_w
Cuts at: 848 1369 2047 2321 2468 2879 848
Size: 521 678 274 147 411 1628
Fragments arranged by size:
1628 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 643 646 643
Size: 3 3656
BseYI C'CCAG_C
Cuts at: 270 274 419 1084 1842 2419 2915 270
Size: 4 145 665 758 577 496 1014
Fragments arranged by size:
1014 758 665 577 496 145 4
BsiEI CG_ry'CG
Cuts at: 104 1201 1247 2455 104
Size: 1097 46 1208 1308
Fragments arranged by size:
1308 1208 1097 46
BsiHKAI G_wGCw'C
Cuts at: 673 1451 1641 2433 673
Size: 778 190 792 1899
Fragments arranged by size:
1899 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 653 673 1284 1377 1451 1641 1703 2433 653
Size: 20 611 93 74 190 62 730 1879
Fragments arranged by size:
1879 730 611 190 93 74 62 20
BspEI T'CCGG_A
Cuts at: 2879 2879
Size: 3659
BspHI T'CATG_A
Cuts at: 2835 2835
Size: 3659
BspMI ACCTGCnnnn'nnnn_
Cuts at: 401 709 923 1225 1606 401
Size: 308 214 302 381 2454
Fragments arranged by size:
2454 381 308 302 214
BsrBI CCG'CTC
Cuts at: 50 119 1951 2005 3489 3654 50
Size: 69 1832 54 1484 165 55
Fragments arranged by size:
1832 1484 165 69 55 54
BsrDI GCAATG_nn'
Cuts at: 1571 1571
Size: 3659
BsrFI r'CCGG_y
Cuts at: 1657 1838 2047 1657
Size: 181 209 3269
Fragments arranged by size:
3269 209 181
BssSI C'ACGA_G
Cuts at: 1930 2288 1930
Size: 358 3301
BstAPI GCAn_nnn'nTGC
Cuts at: 1036 1036
Size: 3659
BstBI TT'CG_AA
Cuts at: 2020 2020
Size: 3659
BstYI r'GATC_y
Cuts at: 1 218 684 1151 1174 1509 1755 2767 2853
Size: 217 466 467 23 335 246 1012 86
Cuts at: 2853 2865 3211 1
Size: 12 346 449
Fragments arranged by size:
1012 467 466 449 346 335 246 217
86 23 12
BtgI C'CryG_G
Cuts at: 240 435 240
Size: 195 3464
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1609 1790 1953 1609
Size: 181 163 3315
Fragments arranged by size:
3315 181 163
BtsI GCAGTG_nn'
Cuts at: 902 902
Size: 3659
DraI TTT'AAA
Cuts at: 658 658
Size: 3659
DrdI GACnn_nn'nnGTC
Cuts at: 574 1365 2223 574
Size: 791 858 2010
Fragments arranged by size:
2010 858 791
EaeI y'GGCC_r
Cuts at: 140 555 1244 1418 1809 1836 3181 140
Size: 415 689 174 391 27 1345 618
Fragments arranged by size:
1345 689 618 415 391 174 27
EagI C'GGCC_G
Cuts at: 1244 1244
Size: 3659
EarI CTCTTCn'nnn_
Cuts at: 209 620 1682 1892 209
Size: 411 1062 210 1976
Fragments arranged by size:
1976 1062 411 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1867 2177 2323 3469 1867
Size: 310 146 1146 2057
Fragments arranged by size:
2057 1146 310 146
EcoICRI GAG'CTC
Cuts at: 671 671
Size: 3659
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 1484 1916 2663 2931 418
Size: 1066 432 747 268 1146
Fragments arranged by size:
1146 1066 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 397 397
Size: 3659
EcoO109I rG'GnC_Cy
Cuts at: 296 798 296
Size: 502 3157
EcoRI G'AATT_C
Cuts at: 663 663
Size: 3659
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1678 1678
Size: 3659
FspI TGC'GCA
Cuts at: 1440 1440
Size: 3659
HaeII r_GCGC'y
Cuts at: 192 426 1093 1341 2363 3342 192
Size: 234 667 248 1022 979 509
Fragments arranged by size:
1022 979 667 509 248 234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1501 1531 1533 1563 1501
Size: 30 2 30 3597
Fragments arranged by size:
3597 30 30 2
HincII GTy'rAC
Cuts at: 489 698 489
Size: 209 3450
KasI G'GCGC_C
Cuts at: 1089 1337 1089
Size: 248 3411
KpnI G_GTAC'C
Cuts at: 679 679
Size: 3659
MluI A'CGCG_T
Cuts at: 3092 3092
Size: 3659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2330 2514 2330
Size: 184 3475
MscI TGG'CCA
Cuts at: 1420 1420
Size: 3659
MslI CAynn'nnrTG
Cuts at: 1648 1775 1648
Size: 127 3532
MspA1I CmG'CkG
Cuts at: 274 770 1084 1444 2457 2702 274
Size: 496 314 360 1013 245 1231
Fragments arranged by size:
1231 1013 496 360 314 245
NaeI GCC'GGC
Cuts at: 1840 1840
Size: 3659
NarI GG'CG_CC
Cuts at: 1090 1338 1090
Size: 248 3411
NcoI C'CATG_G
Cuts at: 240 240
Size: 3659
NgoMIV G'CCGG_C
Cuts at: 1838 1838
Size: 3659
NspI r_CATG'y
Cuts at: 133 712 1212 2119 133
Size: 579 500 907 1673
Fragments arranged by size:
1673 907 579 500
PciI A'CATG_T
Cuts at: 2115 2115
Size: 3659
PflMI CCAn_nnn'nTGG
Cuts at: 426 426
Size: 3659
PmeI GTTT'AAAC
Cuts at: 658 658
Size: 3659
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 310 342 2798 2830 310
Size: 32 2456 32 1139
Fragments arranged by size:
2456 1139 32 32
PstI C_TGCA'G
Cuts at: 706 1391 706
Size: 685 2974
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3659
PvuII CAG'CTG
Cuts at: 274 1084 1444 274
Size: 810 360 2489
Fragments arranged by size:
2489 810 360
RsrII CG'GwC_CG
Cuts at: 1854 1854
Size: 3659
SacI G_AGCT'C
Cuts at: 673 673
Size: 3659
SalI G'TCGA_C
Cuts at: 487 696 487
Size: 209 3450
SapI GCTCTTCn'nnn_
Cuts at: 1682 1892 1682
Size: 210 3449
SbfI CC_TGCA'GG
Cuts at: 706 706
Size: 3659
ScaI AGT'ACT
Cuts at: 3176 3176
Size: 3659
SfcI C'TryA_G
Cuts at: 33 702 1387 2380 2571 33
Size: 669 685 993 191 1121
Fragments arranged by size:
1121 993 685 669 191
SfoI GGC'GCC
Cuts at: 1091 1339 1091
Size: 248 3411
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3659
SmaI CCC'GGG
Cuts at: 681 681
Size: 3659
SmlI C'TyrA_G
Cuts at: 814 1729 2221 2483 814
Size: 915 492 262 1990
Fragments arranged by size:
1990 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3659
SphI G_CATG'C
Cuts at: 712 1212 712
Size: 500 3159
StuI AGG'CCT
Cuts at: 203 203
Size: 3659
StyI C'CwwG_G
Cuts at: 240 793 1147 240
Size: 553 354 2752
Fragments arranged by size:
2752 553 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 169 1242 1375 1834 2043 169
Size: 1073 133 459 209 1785
Fragments arranged by size:
1785 1073 459 209 133
TatI w'GTAC_w
Cuts at: 3174 3174
Size: 3659
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3176 3208 3176
Size: 32 3627
Tth111I GACn'n_nGTC
Cuts at: 1456 1456
Size: 3659
XbaI T'CTAG_A
Cuts at: 690 690
Size: 3659
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1154 1154
Size: 3659
XmaI C'CCGG_G
Cuts at: 679 679
Size: 3659
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI