Restriction map for
pF1KB8865
(Circular) (Six-base) MAPSORT of: pF1KB8865.seq Check: 7857 from: 1 to: 3452
pF1KB8865 3452 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 490 490
Size: 3452
Acc65I G'GTAC_C
Cuts at: 468 468
Size: 3452
AclI AA'CG_TT
Cuts at: 3292 3292
Size: 3452
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1277 1709 2456 2724 1277
Size: 432 747 268 2005
Fragments arranged by size:
2005 747 432 268
AflIII A'CryG_T
Cuts at: 804 1908 2885 804
Size: 1104 977 1371
Fragments arranged by size:
1371 1104 977
AgeI A'CCGG_T
Cuts at: 1840 1840
Size: 3452
AlwNI CAG_nnn'CTG
Cuts at: 757 2324 757
Size: 1567 1885
ApaLI G'TGCA_C
Cuts at: 2222 2222
Size: 3452
ApoI r'AATT_y
Cuts at: 240 456 2805 3061 240
Size: 216 2349 256 631
Fragments arranged by size:
2349 631 256 216
AseI AT'TA_AT
Cuts at: 20 20
Size: 3452
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3452
AvaI C'yCGr_G
Cuts at: 472 472
Size: 3452
BamHI G'GATC_C
Cuts at: 477 477
Size: 3452
BanI G'GyrC_C
Cuts at: 468 882 1130 1165 468
Size: 414 248 35 2755
Fragments arranged by size:
2755 414 248 35
BanII G_rGCy'C
Cuts at: 466 1496 466
Size: 1030 2422
BbeI G_GCGC'C
Cuts at: 886 1134 886
Size: 248 3204
BciVI GTATCCnnnnn_n'
Cuts at: 1350 2111 3452 1350
Size: 761 1341 1350
Fragments arranged by size:
1350 1341 761
BclI T'GATC_A
Cuts at: 972 972
Size: 3452
BglII A'GATC_T
Cuts at: 967 3004 967
Size: 2037 1415
BlpI GC'TnA_GC
Cuts at: 564 564
Size: 3452
Bme1580I G_kGCm'C
Cuts at: 1077 1170 2226 1077
Size: 93 1056 2303
Fragments arranged by size:
2303 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 401 664 745 1080 3224 401
Size: 263 81 335 2144 629
Fragments arranged by size:
2144 629 335 263 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1819 1819
Size: 3452
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 1507 1999 2297 628
Size: 879 492 298 1783
Fragments arranged by size:
1783 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3156 3156
Size: 3452
BsaAI yAC'GTr
Cuts at: 805 805
Size: 3452
BsaBI GATnn'nnATC
Cuts at: 6 966 3003 6
Size: 960 2037 455
Fragments arranged by size:
2037 960 455
BsaHI Gr'CG_yC
Cuts at: 883 1131 1833 3329 883
Size: 248 702 1496 1006
Fragments arranged by size:
1496 1006 702 248
BsaWI w'CCGG_w
Cuts at: 641 1162 1840 2114 2261 2672 641
Size: 521 678 274 147 411 1421
Fragments arranged by size:
1421 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 877 1635 2212 2708 877
Size: 758 577 496 1621
Fragments arranged by size:
1621 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 994 1040 2248 104
Size: 890 46 1208 1308
Fragments arranged by size:
1308 1208 890 46
BsiHKAI G_wGCw'C
Cuts at: 466 1244 1434 2226 466
Size: 778 190 792 1692
Fragments arranged by size:
1692 792 778 190
BsmI GAATG_Cn'
Cuts at: 395 395
Size: 3452
Bsp1286I G_dGCh'C
Cuts at: 466 1077 1170 1244 1434 1496 2226 466
Size: 611 93 74 190 62 730 1692
Fragments arranged by size:
1692 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2672 2672
Size: 3452
BspHI T'CATG_A
Cuts at: 2628 2628
Size: 3452
BspMI ACCTGCnnnn'nnnn_
Cuts at: 502 716 1018 1399 502
Size: 214 302 381 2555
Fragments arranged by size:
2555 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1744 1798 3282 3447 50
Size: 1694 54 1484 165 55
Fragments arranged by size:
1694 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1364 1364
Size: 3452
BsrFI r'CCGG_y
Cuts at: 1450 1631 1840 1450
Size: 181 209 3062
Fragments arranged by size:
3062 209 181
BssSI C'ACGA_G
Cuts at: 1723 2081 1723
Size: 358 3094
BstAPI GCAn_nnn'nTGC
Cuts at: 829 829
Size: 3452
BstBI TT'CG_AA
Cuts at: 1813 1813
Size: 3452
BstXI CCAn_nnnn'nTGG
Cuts at: 121 121
Size: 3452
BstYI r'GATC_y
Cuts at: 1 477 944 967 1302 1548 2560 2646 2658
Size: 476 467 23 335 246 1012 86 12
Cuts at: 2658 3004 1
Size: 346 449
Fragments arranged by size:
1012 476 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 287 287
Size: 3452
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1402 1583 1746 1402
Size: 181 163 3108
Fragments arranged by size:
3108 181 163
BtsI GCAGTG_nn'
Cuts at: 695 695
Size: 3452
DraI TTT'AAA
Cuts at: 128 451 128
Size: 323 3129
DrdI GACnn_nn'nnGTC
Cuts at: 1158 2016 1158
Size: 858 2594
EaeI y'GGCC_r
Cuts at: 1037 1211 1602 1629 2974 1037
Size: 174 391 27 1345 1515
Fragments arranged by size:
1515 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1037 1037
Size: 3452
EarI CTCTTCn'nnn_
Cuts at: 188 1475 1685 188
Size: 1287 210 1955
Fragments arranged by size:
1955 1287 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1660 1970 2116 3262 1660
Size: 310 146 1146 1850
Fragments arranged by size:
1850 1146 310 146
EcoICRI GAG'CTC
Cuts at: 464 464
Size: 3452
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1277 1709 2456 2724 1277
Size: 432 747 268 2005
Fragments arranged by size:
2005 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 356 356
Size: 3452
EcoO109I rG'GnC_Cy
Cuts at: 591 591
Size: 3452
EcoRI G'AATT_C
Cuts at: 456 456
Size: 3452
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1471 1471
Size: 3452
FspI TGC'GCA
Cuts at: 1233 1233
Size: 3452
HaeII r_GCGC'y
Cuts at: 886 1134 2156 3135 886
Size: 248 1022 979 1203
Fragments arranged by size:
1203 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1294 1324 1326 1356 1294
Size: 30 2 30 3390
Fragments arranged by size:
3390 30 30 2
HincII GTy'rAC
Cuts at: 491 491
Size: 3452
KasI G'GCGC_C
Cuts at: 882 1130 882
Size: 248 3204
KpnI G_GTAC'C
Cuts at: 472 472
Size: 3452
MluI A'CGCG_T
Cuts at: 2885 2885
Size: 3452
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 426 2123 2307 426
Size: 1697 184 1571
Fragments arranged by size:
1697 1571 184
MscI TGG'CCA
Cuts at: 1213 1213
Size: 3452
MslI CAynn'nnrTG
Cuts at: 1441 1568 1441
Size: 127 3325
MspA1I CmG'CkG
Cuts at: 563 877 1237 2250 2495 563
Size: 314 360 1013 245 1520
Fragments arranged by size:
1520 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1633 1633
Size: 3452
NarI GG'CG_CC
Cuts at: 883 1131 883
Size: 248 3204
NgoMIV G'CCGG_C
Cuts at: 1631 1631
Size: 3452
NspI r_CATG'y
Cuts at: 505 1005 1912 505
Size: 500 907 2045
Fragments arranged by size:
2045 907 500
PciI A'CATG_T
Cuts at: 1908 1908
Size: 3452
PmeI GTTT'AAAC
Cuts at: 451 451
Size: 3452
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2591 2623 2591
Size: 32 3420
PsiI TTA'TAA
Cuts at: 428 428
Size: 3452
PstI C_TGCA'G
Cuts at: 499 1184 499
Size: 685 2767
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3452
PvuII CAG'CTG
Cuts at: 877 1237 877
Size: 360 3092
RsrII CG'GwC_CG
Cuts at: 1647 1647
Size: 3452
SacI G_AGCT'C
Cuts at: 466 466
Size: 3452
SalI G'TCGA_C
Cuts at: 489 489
Size: 3452
SapI GCTCTTCn'nnn_
Cuts at: 1475 1685 1475
Size: 210 3242
SbfI CC_TGCA'GG
Cuts at: 499 499
Size: 3452
ScaI AGT'ACT
Cuts at: 2969 2969
Size: 3452
SfcI C'TryA_G
Cuts at: 33 353 495 1180 2173 2364 33
Size: 320 142 685 993 191 1121
Fragments arranged by size:
1121 993 685 320 191 142
SfoI GGC'GCC
Cuts at: 884 1132 884
Size: 248 3204
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3452
SmaI CCC'GGG
Cuts at: 474 474
Size: 3452
SmlI C'TyrA_G
Cuts at: 607 1522 2014 2276 607
Size: 915 492 262 1783
Fragments arranged by size:
1783 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3452
SphI G_CATG'C
Cuts at: 505 1005 505
Size: 500 2952
StyI C'CwwG_G
Cuts at: 188 586 940 188
Size: 398 354 2700
Fragments arranged by size:
2700 398 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1035 1168 1627 1836 1035
Size: 133 459 209 2651
Fragments arranged by size:
2651 459 209 133
TatI w'GTAC_w
Cuts at: 228 2967 228
Size: 2739 713
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2969 3001 2969
Size: 32 3420
Tth111I GACn'n_nGTC
Cuts at: 1249 1249
Size: 3452
XbaI T'CTAG_A
Cuts at: 483 483
Size: 3452
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 947 947
Size: 3452
XmaI C'CCGG_G
Cuts at: 472 472
Size: 3452
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI