Restriction map for
pF1KB8854
(Circular) (Six-base) MAPSORT of: pF1KB8854.seq Check: 8286 from: 1 to: 3359
pF1KB8854 3359 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 397 397
Size: 3359
Acc65I G'GTAC_C
Cuts at: 375 375
Size: 3359
AclI AA'CG_TT
Cuts at: 3199 3199
Size: 3359
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1184 1616 2363 2631 1184
Size: 432 747 268 1912
Fragments arranged by size:
1912 747 432 268
AflIII A'CryG_T
Cuts at: 711 1815 2792 711
Size: 1104 977 1278
Fragments arranged by size:
1278 1104 977
AgeI A'CCGG_T
Cuts at: 1747 1747
Size: 3359
AlwNI CAG_nnn'CTG
Cuts at: 664 2231 664
Size: 1567 1792
ApaLI G'TGCA_C
Cuts at: 2129 2129
Size: 3359
ApoI r'AATT_y
Cuts at: 321 363 2712 2968 321
Size: 42 2349 256 712
Fragments arranged by size:
2349 712 256 42
AseI AT'TA_AT
Cuts at: 20 20
Size: 3359
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3359
AvaI C'yCGr_G
Cuts at: 379 379
Size: 3359
BamHI G'GATC_C
Cuts at: 384 384
Size: 3359
BanI G'GyrC_C
Cuts at: 375 789 1037 1072 375
Size: 414 248 35 2662
Fragments arranged by size:
2662 414 248 35
BanII G_rGCy'C
Cuts at: 373 1403 373
Size: 1030 2329
BbeI G_GCGC'C
Cuts at: 793 1041 793
Size: 248 3111
BciVI GTATCCnnnnn_n'
Cuts at: 1257 2018 3359 1257
Size: 761 1341 1257
Fragments arranged by size:
1341 1257 761
BclI T'GATC_A
Cuts at: 879 879
Size: 3359
BglII A'GATC_T
Cuts at: 874 2911 874
Size: 2037 1322
BlpI GC'TnA_GC
Cuts at: 471 471
Size: 3359
Bme1580I G_kGCm'C
Cuts at: 293 348 984 1077 2133 293
Size: 55 636 93 1056 1519
Fragments arranged by size:
1519 1056 636 93 55
BmrI ACTGGGnnnn_n'
Cuts at: 571 652 987 3131 571
Size: 81 335 2144 799
Fragments arranged by size:
2144 799 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1726 1726
Size: 3359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 156
Size: 3359
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 1414 1906 2204 535
Size: 879 492 298 1690
Fragments arranged by size:
1690 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3063 3063
Size: 3359
BsaAI yAC'GTr
Cuts at: 712 712
Size: 3359
BsaBI GATnn'nnATC
Cuts at: 6 873 2910 6
Size: 867 2037 455
Fragments arranged by size:
2037 867 455
BsaHI Gr'CG_yC
Cuts at: 790 1038 1740 3236 790
Size: 248 702 1496 913
Fragments arranged by size:
1496 913 702 248
BsaWI w'CCGG_w
Cuts at: 548 1069 1747 2021 2168 2579 548
Size: 521 678 274 147 411 1328
Fragments arranged by size:
1328 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 154 184 154
Size: 30 3329
BseYI C'CCAG_C
Cuts at: 140 277 784 1542 2119 2615 140
Size: 137 507 758 577 496 884
Fragments arranged by size:
884 758 577 507 496 137
BsiEI CG_ry'CG
Cuts at: 104 901 947 2155 104
Size: 797 46 1208 1308
Fragments arranged by size:
1308 1208 797 46
BsiHKAI G_wGCw'C
Cuts at: 373 1151 1341 2133 373
Size: 778 190 792 1599
Fragments arranged by size:
1599 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 293 348 373 984 1077 1151 1341 1403 2133
Size: 55 25 611 93 74 190 62 730
Cuts at: 2133 293
Size: 1519
Fragments arranged by size:
1519 730 611 190 93 74 62 55
25
BspEI T'CCGG_A
Cuts at: 2579 2579
Size: 3359
BspHI T'CATG_A
Cuts at: 2535 2535
Size: 3359
BspMI ACCTGCnnnn'nnnn_
Cuts at: 409 623 925 1306 409
Size: 214 302 381 2462
Fragments arranged by size:
2462 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1651 1705 3189 3354 50
Size: 1601 54 1484 165 55
Fragments arranged by size:
1601 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1271 1271
Size: 3359
BsrFI r'CCGG_y
Cuts at: 1357 1538 1747 1357
Size: 181 209 2969
Fragments arranged by size:
2969 209 181
BssSI C'ACGA_G
Cuts at: 292 1630 1988 292
Size: 1338 358 1663
Fragments arranged by size:
1663 1338 358
BstAPI GCAn_nnn'nTGC
Cuts at: 736 736
Size: 3359
BstBI TT'CG_AA
Cuts at: 1720 1720
Size: 3359
BstEII G'GTnAC_C
Cuts at: 306 306
Size: 3359
BstYI r'GATC_y
Cuts at: 1 384 851 874 1209 1455 2467 2553 2565
Size: 383 467 23 335 246 1012 86 12
Cuts at: 2565 2911 1
Size: 346 449
Fragments arranged by size:
1012 467 449 383 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3359
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1309 1490 1653 1309
Size: 181 163 3015
Fragments arranged by size:
3015 181 163
BtsI GCAGTG_nn'
Cuts at: 602 602
Size: 3359
DraI TTT'AAA
Cuts at: 358 358
Size: 3359
DrdI GACnn_nn'nnGTC
Cuts at: 1065 1923 1065
Size: 858 2501
EaeI y'GGCC_r
Cuts at: 110 944 1118 1509 1536 2881 110
Size: 834 174 391 27 1345 588
Fragments arranged by size:
1345 834 588 391 174 27
EagI C'GGCC_G
Cuts at: 944 944
Size: 3359
EarI CTCTTCn'nnn_
Cuts at: 1382 1592 1382
Size: 210 3149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1567 1877 2023 3169 1567
Size: 310 146 1146 1757
Fragments arranged by size:
1757 1146 310 146
EcoICRI GAG'CTC
Cuts at: 371 371
Size: 3359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 1184 1616 2363 2631 156
Size: 1028 432 747 268 884
Fragments arranged by size:
1028 884 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 498 498
Size: 3359
EcoRI G'AATT_C
Cuts at: 321 363 321
Size: 42 3317
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1378 1378
Size: 3359
FspI TGC'GCA
Cuts at: 1140 1140
Size: 3359
HaeII r_GCGC'y
Cuts at: 137 793 1041 2063 3042 137
Size: 656 248 1022 979 454
Fragments arranged by size:
1022 979 656 454 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 154 186 1201 1231 1233 1263 154
Size: 32 1015 30 2 30 2250
Fragments arranged by size:
2250 1015 32 30 30 2
HincII GTy'rAC
Cuts at: 398 398
Size: 3359
KasI G'GCGC_C
Cuts at: 789 1037 789
Size: 248 3111
KpnI G_GTAC'C
Cuts at: 379 379
Size: 3359
MluI A'CGCG_T
Cuts at: 2792 2792
Size: 3359
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2030 2214 2030
Size: 184 3175
MscI TGG'CCA
Cuts at: 112 1120 112
Size: 1008 2351
MslI CAynn'nnrTG
Cuts at: 1348 1475 1348
Size: 127 3232
MspA1I CmG'CkG
Cuts at: 470 784 1144 2157 2402 470
Size: 314 360 1013 245 1427
Fragments arranged by size:
1427 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1540 1540
Size: 3359
NarI GG'CG_CC
Cuts at: 790 1038 790
Size: 248 3111
NcoI C'CATG_G
Cuts at: 107 107
Size: 3359
NgoMIV G'CCGG_C
Cuts at: 1538 1538
Size: 3359
NspI r_CATG'y
Cuts at: 412 912 1819 412
Size: 500 907 1952
Fragments arranged by size:
1952 907 500
PciI A'CATG_T
Cuts at: 1815 1815
Size: 3359
PmeI GTTT'AAAC
Cuts at: 358 358
Size: 3359
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2498 2530 2498
Size: 32 3327
PstI C_TGCA'G
Cuts at: 320 406 1091 320
Size: 86 685 2588
Fragments arranged by size:
2588 685 86
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3359
PvuII CAG'CTG
Cuts at: 784 1144 784
Size: 360 2999
RsrII CG'GwC_CG
Cuts at: 1554 1554
Size: 3359
SacI G_AGCT'C
Cuts at: 373 373
Size: 3359
SalI G'TCGA_C
Cuts at: 396 396
Size: 3359
SapI GCTCTTCn'nnn_
Cuts at: 1382 1592 1382
Size: 210 3149
SbfI CC_TGCA'GG
Cuts at: 406 406
Size: 3359
ScaI AGT'ACT
Cuts at: 2876 2876
Size: 3359
SfcI C'TryA_G
Cuts at: 33 195 316 402 1087 2080 2271 33
Size: 162 121 86 685 993 191 1121
Fragments arranged by size:
1121 993 685 191 162 121 86
SfoI GGC'GCC
Cuts at: 791 1039 791
Size: 248 3111
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3359
SmaI CCC'GGG
Cuts at: 381 381
Size: 3359
SmlI C'TyrA_G
Cuts at: 514 1429 1921 2183 514
Size: 915 492 262 1690
Fragments arranged by size:
1690 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3359
SphI G_CATG'C
Cuts at: 412 912 412
Size: 500 2859
StyI C'CwwG_G
Cuts at: 107 493 847 107
Size: 386 354 2619
Fragments arranged by size:
2619 386 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 942 1075 1534 1743 942
Size: 133 459 209 2558
Fragments arranged by size:
2558 459 209 133
TatI w'GTAC_w
Cuts at: 2874 2874
Size: 3359
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2876 2908 2876
Size: 32 3327
Tth111I GACn'n_nGTC
Cuts at: 1156 1156
Size: 3359
XbaI T'CTAG_A
Cuts at: 390 390
Size: 3359
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 854 854
Size: 3359
XmaI C'CCGG_G
Cuts at: 379 379
Size: 3359
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI