Restriction map for
pF1KB8838
(Circular) (Six-base) MAPSORT of: pF1KB8838.seq Check: 8103 from: 1 to: 4502
pF1KB8838 4502 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 426 426
Size: 4502
AatII G_ACGT'C
Cuts at: 1238 1238
Size: 4502
AccI GT'mk_AC
Cuts at: 1540 1540
Size: 4502
Acc65I G'GTAC_C
Cuts at: 1518 1518
Size: 4502
AclI AA'CG_TT
Cuts at: 4342 4342
Size: 4502
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 2327 2759 3506 3774 292
Size: 2035 432 747 268 1020
Fragments arranged by size:
2035 1020 747 432 268
AflIII A'CryG_T
Cuts at: 1854 2958 3935 1854
Size: 1104 977 2421
Fragments arranged by size:
2421 1104 977
AgeI A'CCGG_T
Cuts at: 1207 2890 1207
Size: 1683 2819
AhdI GACnn_n'nnGTC
Cuts at: 483 483
Size: 4502
AleI CACnn'nnGTG
Cuts at: 1162 1162
Size: 4502
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1180 1180
Size: 4502
AlwNI CAG_nnn'CTG
Cuts at: 354 423 936 1334 1807 3374 354
Size: 69 513 398 473 1567 1482
Fragments arranged by size:
1567 1482 513 473 398 69
ApaI G_GGCC'C
Cuts at: 301 909 1245 301
Size: 608 336 3558
Fragments arranged by size:
3558 608 336
ApaLI G'TGCA_C
Cuts at: 3272 3272
Size: 4502
ApoI r'AATT_y
Cuts at: 1249 1506 3855 4111 1249
Size: 257 2349 256 1640
Fragments arranged by size:
2349 1640 257 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4502
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4502
AvaI C'yCGr_G
Cuts at: 358 1145 1522 358
Size: 787 377 3338
Fragments arranged by size:
3338 787 377
BamHI G'GATC_C
Cuts at: 654 1527 654
Size: 873 3629
BanI G'GyrC_C
Cuts at: 163 448 1204 1518 1932 2180 2215 163
Size: 285 756 314 414 248 35 2450
Fragments arranged by size:
2450 756 414 314 285 248 35
BanII G_rGCy'C
Cuts at: 175 301 909 1081 1219 1245 1516 2546 175
Size: 126 608 172 138 26 271 1030 2131
Fragments arranged by size:
2131 1030 608 271 172 138 126 26
BbeI G_GCGC'C
Cuts at: 167 1936 2184 167
Size: 1769 248 2485
Fragments arranged by size:
2485 1769 248
BciVI GTATCCnnnnn_n'
Cuts at: 2400 3161 4502 2400
Size: 761 1341 2400
Fragments arranged by size:
2400 1341 761
BclI T'GATC_A
Cuts at: 2022 2022
Size: 4502
BglI GCCn_nnn'nGGC
Cuts at: 535 535
Size: 4502
BglII A'GATC_T
Cuts at: 2017 4054 2017
Size: 2037 2465
BlpI GC'TnA_GC
Cuts at: 1174 1614 1174
Size: 440 4062
Bme1580I G_kGCm'C
Cuts at: 301 394 909 1207 1245 2127 2220 3276 301
Size: 93 515 298 38 882 93 1056 1527
Fragments arranged by size:
1527 1056 882 515 298 93 93 38
BmgBI CAC'GTC
Cuts at: 480 1092 480
Size: 612 3890
BmrI ACTGGGnnnn_n'
Cuts at: 881 946 1714 1795 2130 4274 881
Size: 65 768 81 335 2144 1109
Fragments arranged by size:
2144 1109 768 335 81 65
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1354 2869 1354
Size: 1515 2987
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 403 414 1276 1300 1512 295
Size: 108 11 862 24 212 3285
Fragments arranged by size:
3285 862 212 108 24 11
Bpu10I CC'TnA_GC
Cuts at: 1312 1312
Size: 4502
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 1678 2557 3049 3347 208
Size: 1470 879 492 298 1363
Fragments arranged by size:
1470 1363 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 826 4206 826
Size: 3380 1122
BsaAI yAC'GTr
Cuts at: 1855 1855
Size: 4502
BsaBI GATnn'nnATC
Cuts at: 6 2016 4053 6
Size: 2010 2037 455
Fragments arranged by size:
2037 2010 455
BsaHI Gr'CG_yC
Cuts at: 164 1235 1933 2181 2883 4379 164
Size: 1071 698 248 702 1496 287
Fragments arranged by size:
1496 1071 702 698 287 248
BsaWI w'CCGG_w
Cuts at: 1207 1691 2212 2890 3164 3311 3722 1207
Size: 484 521 678 274 147 411 1987
Fragments arranged by size:
1987 678 521 484 411 274 147
BseYI C'CCAG_C
Cuts at: 113 387 1444 1927 2685 3262 3758 113
Size: 274 1057 483 758 577 496 857
Fragments arranged by size:
1057 857 758 577 496 483 274
BsiEI CG_ry'CG
Cuts at: 104 155 241 675 1171 2044 2090 3298 104
Size: 51 86 434 496 873 46 1208 1308
Fragments arranged by size:
1308 1208 873 496 434 86 51 46
BsiHKAI G_wGCw'C
Cuts at: 175 1516 2294 2484 3276 175
Size: 1341 778 190 792 1401
Fragments arranged by size:
1401 1341 792 778 190
BsmI GAATG_Cn'
Cuts at: 503 503
Size: 4502
BsmBI CGTCTCn'nnnn_
Cuts at: 1098 1098
Size: 4502
Bsp1286I G_dGCh'C
Cuts at: 175 301 394 909 1081 1207 1219 1245 1516
Size: 126 93 515 172 126 12 26 271
Cuts at: 1516 2127 2220 2294 2484 2546 3276 175
Size: 611 93 74 190 62 730 1401
Fragments arranged by size:
1401 730 611 515 271 190 172 126
126 93 93 74 62 26 12
BspEI T'CCGG_A
Cuts at: 3722 3722
Size: 4502
BspHI T'CATG_A
Cuts at: 3678 3678
Size: 4502
BspMI ACCTGCnnnn'nnnn_
Cuts at: 426 1305 1552 1766 2068 2449 426
Size: 879 247 214 302 381 2479
Fragments arranged by size:
2479 879 381 302 247 214
BsrBI CCG'CTC
Cuts at: 50 737 943 2794 2848 4332 4497 50
Size: 687 206 1851 54 1484 165 55
Fragments arranged by size:
1851 1484 687 206 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2414 2414
Size: 4502
BsrFI r'CCGG_y
Cuts at: 240 604 1207 2500 2681 2890 240
Size: 364 603 1293 181 209 1852
Fragments arranged by size:
1852 1293 603 364 209 181
BssHII G'CGCG_C
Cuts at: 499 499
Size: 4502
BssSI C'ACGA_G
Cuts at: 2773 3131 2773
Size: 358 4144
BstAPI GCAn_nnn'nTGC
Cuts at: 1879 1879
Size: 4502
BstBI TT'CG_AA
Cuts at: 2863 2863
Size: 4502
BstXI CCAn_nnnn'nTGG
Cuts at: 438 627 438
Size: 189 4313
BstYI r'GATC_y
Cuts at: 1 654 695 1104 1527 1994 2017 2352 2598
Size: 653 41 409 423 467 23 335 246
Cuts at: 2598 3610 3696 3708 4054 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 653 467 449 423 409 346 335
246 86 41 23 12
BtgI C'CryG_G
Cuts at: 107 149 678 1073 1470 107
Size: 42 529 395 397 3139
Fragments arranged by size:
3139 529 397 395 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 480 2452 2633 2796 480
Size: 1972 181 163 2186
Fragments arranged by size:
2186 1972 181 163
BtsI GCAGTG_nn'
Cuts at: 1407 1745 1407
Size: 338 4164
DraI TTT'AAA
Cuts at: 1501 1501
Size: 4502
DraIII CAC_nnn'GTG
Cuts at: 455 455
Size: 4502
DrdI GACnn_nn'nnGTC
Cuts at: 2208 3066 2208
Size: 858 3644
EaeI y'GGCC_r
Cuts at: 238 599 629 681 2087 2261 2652 2679 4024
Size: 361 30 52 1406 174 391 27 1345
Cuts at: 4024 238
Size: 716
Fragments arranged by size:
1406 1345 716 391 361 174 52 30
27
EagI C'GGCC_G
Cuts at: 238 2087 238
Size: 1849 2653
EarI CTCTTCn'nnn_
Cuts at: 1231 1293 2525 2735 1231
Size: 62 1232 210 2998
Fragments arranged by size:
2998 1232 210 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 783 1260 2710 3020 3166 4312 783
Size: 477 1450 310 146 1146 973
Fragments arranged by size:
1450 1146 973 477 310 146
EcoICRI GAG'CTC
Cuts at: 173 1514 173
Size: 1341 3161
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 295 403 414 1276 1300 1512 2327 2759
Size: 3 108 11 862 24 212 815 432
Cuts at: 2759 3506 3774 292
Size: 747 268 1020
Fragments arranged by size:
1020 862 815 747 432 268 212 108
24 11 3
EcoO109I rG'GnC_Cy
Cuts at: 297 298 906 1294 1450 1641 297
Size: 1 608 388 156 191 3158
Fragments arranged by size:
3158 608 388 191 156 1
EcoRI G'AATT_C
Cuts at: 1506 1506
Size: 4502
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2521 2521
Size: 4502
FspI TGC'GCA
Cuts at: 2283 2283
Size: 4502
HaeII r_GCGC'y
Cuts at: 148 167 378 763 1936 2184 3206 4185 148
Size: 19 211 385 1173 248 1022 979 465
Fragments arranged by size:
1173 1022 979 465 385 248 211 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 469 501 1045 1077 2344 2374 2376 2406 469
Size: 32 544 32 1267 30 2 30 2565
Fragments arranged by size:
2565 1267 544 32 32 30 30 2
HincII GTy'rAC
Cuts at: 399 1541 399
Size: 1142 3360
KasI G'GCGC_C
Cuts at: 163 1932 2180 163
Size: 1769 248 2485
Fragments arranged by size:
2485 1769 248
KpnI G_GTAC'C
Cuts at: 1522 1522
Size: 4502
MluI A'CGCG_T
Cuts at: 3935 3935
Size: 4502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3173 3357 3173
Size: 184 4318
MscI TGG'CCA
Cuts at: 601 631 683 2263 601
Size: 30 52 1580 2840
Fragments arranged by size:
2840 1580 52 30
MslI CAynn'nnrTG
Cuts at: 1162 1198 1381 2491 2618 1162
Size: 36 183 1110 127 3046
Fragments arranged by size:
3046 1110 183 127 36
MspA1I CmG'CkG
Cuts at: 151 199 216 642 1613 1927 2287 3300 3545
Size: 48 17 426 971 314 360 1013 245
Cuts at: 3545 151
Size: 1108
Fragments arranged by size:
1108 1013 971 426 360 314 245 48
17
NaeI GCC'GGC
Cuts at: 242 2683 242
Size: 2441 2061
NarI GG'CG_CC
Cuts at: 164 1933 2181 164
Size: 1769 248 2485
Fragments arranged by size:
2485 1769 248
NcoI C'CATG_G
Cuts at: 107 678 1470 107
Size: 571 792 3139
Fragments arranged by size:
3139 792 571
NgoMIV G'CCGG_C
Cuts at: 240 2681 240
Size: 2441 2061
NspI r_CATG'y
Cuts at: 1555 2055 2962 1555
Size: 500 907 3095
Fragments arranged by size:
3095 907 500
PasI CC'CwG_GG
Cuts at: 869 869
Size: 4502
PciI A'CATG_T
Cuts at: 2958 2958
Size: 4502
PflMI CCAn_nnn'nTGG
Cuts at: 936 936
Size: 4502
PfoI T'CCnGG_A
Cuts at: 1025 1100 1253 1025
Size: 75 153 4274
Fragments arranged by size:
4274 153 75
PmeI GTTT'AAAC
Cuts at: 1501 1501
Size: 4502
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3641 3673 3641
Size: 32 4470
PpuMI rG'GwC_Cy
Cuts at: 1294 1294
Size: 4502
PspOMI G'GGCC_C
Cuts at: 297 905 1241 297
Size: 608 336 3558
Fragments arranged by size:
3558 608 336
PstI C_TGCA'G
Cuts at: 1464 1549 2234 1464
Size: 85 685 3732
Fragments arranged by size:
3732 685 85
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4502
PvuII CAG'CTG
Cuts at: 642 1927 2287 642
Size: 1285 360 2857
Fragments arranged by size:
2857 1285 360
RsrII CG'GwC_CG
Cuts at: 2697 2697
Size: 4502
SacI G_AGCT'C
Cuts at: 175 1516 175
Size: 1341 3161
SacII CC_GC'GG
Cuts at: 152 152
Size: 4502
SalI G'TCGA_C
Cuts at: 1539 1539
Size: 4502
SapI GCTCTTCn'nnn_
Cuts at: 1231 2525 2735 1231
Size: 1294 210 2998
Fragments arranged by size:
2998 1294 210
SbfI CC_TGCA'GG
Cuts at: 1464 1549 1464
Size: 85 4417
ScaI AGT'ACT
Cuts at: 4019 4019
Size: 4502
SfcI C'TryA_G
Cuts at: 33 1460 1545 2230 3223 3414 33
Size: 1427 85 685 993 191 1121
Fragments arranged by size:
1427 1121 993 685 191 85
SfiI GGCCn_nnn'nGGCC
Cuts at: 535 535
Size: 4502
SfoI GGC'GCC
Cuts at: 165 1934 2182 165
Size: 1769 248 2485
Fragments arranged by size:
2485 1769 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4502
SgrAI Cr'CCGG_yG
Cuts at: 604 604
Size: 4502
SmaI CCC'GGG
Cuts at: 1524 1524
Size: 4502
SmlI C'TyrA_G
Cuts at: 223 1145 1657 2572 3064 3326 223
Size: 922 512 915 492 262 1399
Fragments arranged by size:
1399 922 915 512 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4502
SphI G_CATG'C
Cuts at: 1555 2055 1555
Size: 500 4002
StuI AGG'CCT
Cuts at: 1466 1487 1466
Size: 21 4481
StyI C'CwwG_G
Cuts at: 107 678 950 1470 1636 1990 107
Size: 571 272 520 166 354 2619
Fragments arranged by size:
2619 571 520 354 272 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1347 1420 2085 2218 2677 2886 1347
Size: 73 665 133 459 209 2963
Fragments arranged by size:
2963 665 459 209 133 73
TatI w'GTAC_w
Cuts at: 4017 4017
Size: 4502
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 646 678 1021 1053 4019 4051 646
Size: 32 343 32 2966 32 1097
Fragments arranged by size:
2966 1097 343 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2299 2299
Size: 4502
XbaI T'CTAG_A
Cuts at: 1533 1533
Size: 4502
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 319 594 1997 319
Size: 275 1403 2824
Fragments arranged by size:
2824 1403 275
XhoI C'TCGA_G
Cuts at: 1145 1145
Size: 4502
XmaI C'CCGG_G
Cuts at: 1522 1522
Size: 4502
XmnI GAAnn'nnTTC
Cuts at: 1385 1385
Size: 4502
ZraI GAC'GTC
Cuts at: 1236 1236
Size: 4502
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AloI AscI AvrII BaeI BbsI BbvCI
BcgI BfrBI BmtI BsaXI BseRI BsgI BsiWI BsrGI
BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspXI PsrI SanDI
SexAI SnaBI SrfI SspI SwaI