Restriction map for
pF1KB8837
(Circular) (Six-base) MAPSORT of: pF1KB8837.seq Check: 5487 from: 1 to: 4502
pF1KB8837 4502 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1050 1066 1050
Size: 16 4486
AccI GT'mk_AC
Cuts at: 494 1250 1540 494
Size: 756 290 3456
Fragments arranged by size:
3456 756 290
Acc65I G'GTAC_C
Cuts at: 1518 1518
Size: 4502
AclI AA'CG_TT
Cuts at: 4342 4342
Size: 4502
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 483 645 2327 2759 3506 3774 483
Size: 162 1682 432 747 268 1211
Fragments arranged by size:
1682 1211 747 432 268 162
AflII C'TTAA_G
Cuts at: 1450 1450
Size: 4502
AflIII A'CryG_T
Cuts at: 1854 2958 3935 1854
Size: 1104 977 2421
Fragments arranged by size:
2421 1104 977
AgeI A'CCGG_T
Cuts at: 2890 2890
Size: 4502
AleI CACnn'nnGTG
Cuts at: 613 790 613
Size: 177 4325
AlwNI CAG_nnn'CTG
Cuts at: 243 945 1807 3374 243
Size: 702 862 1567 1371
Fragments arranged by size:
1567 1371 862 702
ApaLI G'TGCA_C
Cuts at: 3272 3272
Size: 4502
ApoI r'AATT_y
Cuts at: 1506 3855 4111 1506
Size: 2349 256 1897
Fragments arranged by size:
2349 1897 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4502
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4502
AvaI C'yCGr_G
Cuts at: 1522 1522
Size: 4502
BamHI G'GATC_C
Cuts at: 1527 1527
Size: 4502
BanI G'GyrC_C
Cuts at: 132 827 1165 1518 1932 2180 2215 132
Size: 695 338 353 414 248 35 2419
Fragments arranged by size:
2419 695 414 353 338 248 35
BanII G_rGCy'C
Cuts at: 1390 1516 2546 1390
Size: 126 1030 3346
Fragments arranged by size:
3346 1030 126
BbeI G_GCGC'C
Cuts at: 831 1169 1936 2184 831
Size: 338 767 248 3149
Fragments arranged by size:
3149 767 338 248
BbvCI CC'TCA_GC
Cuts at: 1286 1286
Size: 4502
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1419 1453 1419
Size: 34 4468
BciVI GTATCCnnnnn_n'
Cuts at: 2400 3161 4502 2400
Size: 761 1341 2400
Fragments arranged by size:
2400 1341 761
BclI T'GATC_A
Cuts at: 2022 2022
Size: 4502
BglI GCCn_nnn'nGGC
Cuts at: 727 836 1051 727
Size: 109 215 4178
Fragments arranged by size:
4178 215 109
BglII A'GATC_T
Cuts at: 2017 4054 2017
Size: 2037 2465
BlpI GC'TnA_GC
Cuts at: 1614 1614
Size: 4502
Bme1580I G_kGCm'C
Cuts at: 2127 2220 3276 2127
Size: 93 1056 3353
Fragments arranged by size:
3353 1056 93
BmgBI CAC'GTC
Cuts at: 426 426
Size: 4502
BmrI ACTGGGnnnn_n'
Cuts at: 1146 1714 1795 2130 4274 1146
Size: 568 81 335 2144 1374
Fragments arranged by size:
2144 1374 568 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2869 2869
Size: 4502
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 220 292 358 443 886 1246 1339 199
Size: 21 72 66 85 443 360 93 3362
Fragments arranged by size:
3362 443 360 93 85 72 66 21
Bpu10I CC'TnA_GC
Cuts at: 1286 1286
Size: 4502
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 895 1678 2557 3049 3347 895
Size: 783 879 492 298 2050
Fragments arranged by size:
2050 879 783 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 373 531 547 686 1125 4206 373
Size: 158 16 139 439 3081 669
Fragments arranged by size:
3081 669 439 158 139 16
BsaAI yAC'GTr
Cuts at: 1855 1855
Size: 4502
BsaBI GATnn'nnATC
Cuts at: 6 2016 4053 6
Size: 2010 2037 455
Fragments arranged by size:
2037 2010 455
BsaHI Gr'CG_yC
Cuts at: 281 371 828 932 1166 1292 1933 2181 2883
Size: 90 457 104 234 126 641 248 702
Cuts at: 2883 4379 281
Size: 1496 404
Fragments arranged by size:
1496 702 641 457 404 248 234 126
104 90
BsaWI w'CCGG_w
Cuts at: 1691 2212 2890 3164 3311 3722 1691
Size: 521 678 274 147 411 2471
Fragments arranged by size:
2471 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 802 832 802
Size: 30 4472
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 157 157
Size: 4502
BseYI C'CCAG_C
Cuts at: 644 1295 1927 2685 3262 3758 644
Size: 651 632 758 577 496 1388
Fragments arranged by size:
1388 758 651 632 577 496
BsiEI CG_ry'CG
Cuts at: 104 652 968 2044 2090 3298 104
Size: 548 316 1076 46 1208 1308
Fragments arranged by size:
1308 1208 1076 548 316 46
BsiHKAI G_wGCw'C
Cuts at: 809 1516 2294 2484 3276 809
Size: 707 778 190 792 2035
Fragments arranged by size:
2035 792 778 707 190
Bsp1286I G_dGCh'C
Cuts at: 809 1390 1516 2127 2220 2294 2484 2546 3276
Size: 581 126 611 93 74 190 62 730
Cuts at: 3276 809
Size: 2035
Fragments arranged by size:
2035 730 611 581 190 126 93 74
62
BspEI T'CCGG_A
Cuts at: 3722 3722
Size: 4502
BspHI T'CATG_A
Cuts at: 3678 3678
Size: 4502
BspMI ACCTGCnnnn'nnnn_
Cuts at: 449 480 821 1050 1066 1091 1435 1552 1766
Size: 31 341 229 16 25 344 117 214
Cuts at: 1766 2068 2449 449
Size: 302 381 2502
Fragments arranged by size:
2502 381 344 341 302 229 214 117
31 25 16
BsrBI CCG'CTC
Cuts at: 50 708 1100 2794 2848 4332 4497 50
Size: 658 392 1694 54 1484 165 55
Fragments arranged by size:
1694 1484 658 392 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2414 2414
Size: 4502
BsrFI r'CCGG_y
Cuts at: 451 721 765 824 948 2500 2681 2890 451
Size: 270 44 59 124 1552 181 209 2063
Fragments arranged by size:
2063 1552 270 209 181 124 59 44
BssSI C'ACGA_G
Cuts at: 2773 3131 2773
Size: 358 4144
BstAPI GCAn_nnn'nTGC
Cuts at: 813 1879 813
Size: 1066 3436
BstBI TT'CG_AA
Cuts at: 2863 2863
Size: 4502
BstEII G'GTnAC_C
Cuts at: 208 1078 208
Size: 870 3632
BstXI CCAn_nnnn'nTGG
Cuts at: 162 1176 162
Size: 1014 3488
BstYI r'GATC_y
Cuts at: 1 572 686 1527 1994 2017 2352 2598 3610
Size: 571 114 841 467 23 335 246 1012
Cuts at: 3610 3696 3708 4054 1
Size: 86 12 346 449
Fragments arranged by size:
1012 841 571 467 449 346 335 246
114 86 23 12
BtgI C'CryG_G
Cuts at: 107 128 254 107
Size: 21 126 4355
Fragments arranged by size:
4355 126 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2452 2633 2796 2452
Size: 181 163 4158
Fragments arranged by size:
4158 181 163
BtsI GCAGTG_nn'
Cuts at: 997 1745 997
Size: 748 3754
DraI TTT'AAA
Cuts at: 1501 1501
Size: 4502
DraIII CAC_nnn'GTG
Cuts at: 1003 1003
Size: 4502
DrdI GACnn_nn'nnGTC
Cuts at: 2208 3066 2208
Size: 858 3644
EaeI y'GGCC_r
Cuts at: 164 563 632 649 965 1043 1052 1109 1358
Size: 399 69 17 316 78 9 57 249
Cuts at: 1358 2087 2261 2652 2679 4024 164
Size: 729 174 391 27 1345 642
Fragments arranged by size:
1345 729 642 399 391 316 249 174
78 69 57 27 17 9
EagI C'GGCC_G
Cuts at: 649 965 2087 649
Size: 316 1122 3064
Fragments arranged by size:
3064 1122 316
EarI CTCTTCn'nnn_
Cuts at: 1484 2525 2735 1484
Size: 1041 210 3251
Fragments arranged by size:
3251 1041 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 256 2710 3020 3166 4312 256
Size: 2454 310 146 1146 446
Fragments arranged by size:
2454 1146 446 310 146
EcoICRI GAG'CTC
Cuts at: 1514 1514
Size: 4502
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 220 292 358 443 483 645 886 1246
Size: 21 72 66 85 40 162 241 360
Cuts at: 1246 1339 2327 2759 3506 3774 199
Size: 93 988 432 747 268 927
Fragments arranged by size:
988 927 747 432 360 268 241 162
93 85 72 66 40 21
EcoNI CCTnn'n_nnAGG
Cuts at: 217 487 580 1191 217
Size: 270 93 611 3528
Fragments arranged by size:
3528 611 270 93
EcoO109I rG'GnC_Cy
Cuts at: 183 348 438 624 942 1314 1641 183
Size: 165 90 186 318 372 327 3044
Fragments arranged by size:
3044 372 327 318 186 165 90
EcoRI G'AATT_C
Cuts at: 1506 1506
Size: 4502
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 615 2521 615
Size: 1906 2596
FspI TGC'GCA
Cuts at: 190 607 2283 190
Size: 417 1676 2409
Fragments arranged by size:
2409 1676 417
FspAI rTGC'GCAy
Cuts at: 607 607
Size: 4502
HaeII r_GCGC'y
Cuts at: 831 1169 1247 1936 2184 3206 4185 831
Size: 338 78 689 248 1022 979 1148
Fragments arranged by size:
1148 1022 979 689 338 248 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 802 834 1021 1053 1072 1104 2344 2374 2376
Size: 32 187 32 19 32 1240 30 2
Cuts at: 2376 2406 802
Size: 30 2898
Fragments arranged by size:
2898 1240 187 32 32 32 30 30
19 2
HincII GTy'rAC
Cuts at: 1541 1541
Size: 4502
KasI G'GCGC_C
Cuts at: 827 1165 1932 2180 827
Size: 338 767 248 3149
Fragments arranged by size:
3149 767 338 248
KpnI G_GTAC'C
Cuts at: 1522 1522
Size: 4502
MluI A'CGCG_T
Cuts at: 3935 3935
Size: 4502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 779 1184 3173 3357 779
Size: 405 1989 184 1924
Fragments arranged by size:
1989 1924 405 184
MscI TGG'CCA
Cuts at: 166 1045 1111 1360 2263 166
Size: 879 66 249 903 2405
Fragments arranged by size:
2405 903 879 249 66
MslI CAynn'nnrTG
Cuts at: 160 613 790 1174 1204 2491 2618 160
Size: 453 177 384 30 1287 127 2044
Fragments arranged by size:
2044 1287 453 384 177 127 30
MspA1I CmG'CkG
Cuts at: 539 568 648 673 1469 1613 1927 2287 3300
Size: 29 80 25 796 144 314 360 1013
Cuts at: 3300 3545 539
Size: 245 1496
Fragments arranged by size:
1496 1013 796 360 314 245 144 80
29 25
NaeI GCC'GGC
Cuts at: 453 723 767 2683 453
Size: 270 44 1916 2272
Fragments arranged by size:
2272 1916 270 44
NarI GG'CG_CC
Cuts at: 828 1166 1933 2181 828
Size: 338 767 248 3149
Fragments arranged by size:
3149 767 338 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4502
NgoMIV G'CCGG_C
Cuts at: 451 721 765 2681 451
Size: 270 44 1916 2272
Fragments arranged by size:
2272 1916 270 44
NotI GC'GGCC_GC
Cuts at: 965 965
Size: 4502
NspI r_CATG'y
Cuts at: 1555 2055 2962 1555
Size: 500 907 3095
Fragments arranged by size:
3095 907 500
PasI CC'CwG_GG
Cuts at: 955 955
Size: 4502
PciI A'CATG_T
Cuts at: 2958 2958
Size: 4502
PfoI T'CCnGG_A
Cuts at: 236 236
Size: 4502
PmeI GTTT'AAAC
Cuts at: 1501 1501
Size: 4502
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3641 3673 3641
Size: 32 4470
PpuMI rG'GwC_Cy
Cuts at: 348 438 624 348
Size: 90 186 4226
Fragments arranged by size:
4226 186 90
PstI C_TGCA'G
Cuts at: 683 1432 1549 2234 683
Size: 749 117 685 2951
Fragments arranged by size:
2951 749 685 117
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4502
PvuII CAG'CTG
Cuts at: 1469 1927 2287 1469
Size: 458 360 3684
Fragments arranged by size:
3684 458 360
RsrII CG'GwC_CG
Cuts at: 2697 2697
Size: 4502
SacI G_AGCT'C
Cuts at: 1516 1516
Size: 4502
SalI G'TCGA_C
Cuts at: 1539 1539
Size: 4502
SapI GCTCTTCn'nnn_
Cuts at: 2525 2735 2525
Size: 210 4292
SbfI CC_TGCA'GG
Cuts at: 683 1432 1549 683
Size: 749 117 3636
Fragments arranged by size:
3636 749 117
ScaI AGT'ACT
Cuts at: 4019 4019
Size: 4502
SfcI C'TryA_G
Cuts at: 33 679 1428 1545 2230 3223 3414 33
Size: 646 749 117 685 993 191 1121
Fragments arranged by size:
1121 993 749 685 646 191 117
SfiI GGCCn_nnn'nGGCC
Cuts at: 1051 1051
Size: 4502
SfoI GGC'GCC
Cuts at: 829 1167 1934 2182 829
Size: 338 767 248 3149
Fragments arranged by size:
3149 767 338 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4502
SgrAI Cr'CCGG_yG
Cuts at: 824 824
Size: 4502
SmaI CCC'GGG
Cuts at: 1524 1524
Size: 4502
SmlI C'TyrA_G
Cuts at: 910 1450 1657 2572 3064 3326 910
Size: 540 207 915 492 262 2086
Fragments arranged by size:
2086 915 540 492 262 207
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4502
SphI G_CATG'C
Cuts at: 1555 2055 1555
Size: 500 4002
StuI AGG'CCT
Cuts at: 871 871
Size: 4502
StyI C'CwwG_G
Cuts at: 107 167 611 1636 1990 107
Size: 60 444 1025 354 2619
Fragments arranged by size:
2619 1025 444 354 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 425 658 1080 2085 2218 2677 2886 425
Size: 233 422 1005 133 459 209 2041
Fragments arranged by size:
2041 1005 459 422 233 209 133
TatI w'GTAC_w
Cuts at: 1454 4017 1454
Size: 2563 1939
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4019 4051 4019
Size: 32 4470
Tth111I GACn'n_nGTC
Cuts at: 2299 2299
Size: 4502
XbaI T'CTAG_A
Cuts at: 1533 1533
Size: 4502
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 174 1000 1018 1997 174
Size: 826 18 979 2679
Fragments arranged by size:
2679 979 826 18
XmaI C'CCGG_G
Cuts at: 1522 1522
Size: 4502
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NotI NspI PasI PciI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AlfI AloI ApaI AscI AvrII
BaeI BbsI BfrBI BmtI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoRV FseI
HindIII HpaI MfeI NdeI NheI NruI NsiI PacI
PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI