Restriction map for
pF1KB8816
(Circular) (Six-base) MAPSORT of: pF1KB8816.seq Check: 2763 from: 1 to: 4250
pF1KB8816 4250 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 713 1288 713
Size: 575 3675
Acc65I G'GTAC_C
Cuts at: 332 1266 332
Size: 934 3316
AclI AA'CG_TT
Cuts at: 4090 4090
Size: 4250
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 380 874 2075 2507 3254 3522 380
Size: 494 1201 432 747 268 1108
Fragments arranged by size:
1201 1108 747 494 432 268
AflIII A'CryG_T
Cuts at: 1602 2706 3683 1602
Size: 1104 977 2169
Fragments arranged by size:
2169 1104 977
AgeI A'CCGG_T
Cuts at: 2638 2638
Size: 4250
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 845 845
Size: 4250
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1211 1243 1211
Size: 32 4218
AlwNI CAG_nnn'CTG
Cuts at: 897 1555 3122 897
Size: 658 1567 2025
Fragments arranged by size:
2025 1567 658
ApaI G_GGCC'C
Cuts at: 957 957
Size: 4250
ApaLI G'TGCA_C
Cuts at: 3020 3020
Size: 4250
ApoI r'AATT_y
Cuts at: 912 1123 1198 1254 3603 3859 912
Size: 211 75 56 2349 256 1303
Fragments arranged by size:
2349 1303 256 211 75 56
AseI AT'TA_AT
Cuts at: 20 20
Size: 4250
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4250
AvaI C'yCGr_G
Cuts at: 404 629 1270 404
Size: 225 641 3384
Fragments arranged by size:
3384 641 225
BamHI G'GATC_C
Cuts at: 633 1275 633
Size: 642 3608
BanI G'GyrC_C
Cuts at: 163 332 1266 1680 1928 1963 163
Size: 169 934 414 248 35 2450
Fragments arranged by size:
2450 934 414 248 169 35
BanII G_rGCy'C
Cuts at: 348 957 979 1264 2294 348
Size: 609 22 285 1030 2304
Fragments arranged by size:
2304 1030 609 285 22
BbeI G_GCGC'C
Cuts at: 167 1684 1932 167
Size: 1517 248 2485
Fragments arranged by size:
2485 1517 248
BbvCI CC'TCA_GC
Cuts at: 224 224
Size: 4250
BciVI GTATCCnnnnn_n'
Cuts at: 2148 2909 4250 2148
Size: 761 1341 2148
Fragments arranged by size:
2148 1341 761
BclI T'GATC_A
Cuts at: 1770 1770
Size: 4250
BfrBI ATG'CAT
Cuts at: 565 565
Size: 4250
BglII A'GATC_T
Cuts at: 1765 3802 1765
Size: 2037 2213
BlpI GC'TnA_GC
Cuts at: 1362 1362
Size: 4250
Bme1580I G_kGCm'C
Cuts at: 411 957 1875 1968 3024 411
Size: 546 918 93 1056 1637
Fragments arranged by size:
1637 1056 918 546 93
BmgBI CAC'GTC
Cuts at: 412 412
Size: 4250
BmrI ACTGGGnnnn_n'
Cuts at: 982 1462 1543 1878 4022 982
Size: 480 81 335 2144 1210
Fragments arranged by size:
2144 1210 480 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2617 2617
Size: 4250
Bpu10I CC'TnA_GC
Cuts at: 224 949 224
Size: 725 3525
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 452 696 1426 2305 2797 3095 452
Size: 244 730 879 492 298 1607
Fragments arranged by size:
1607 879 730 492 298 244
BsaI GGTCTCn'nnnn_
Cuts at: 604 3954 604
Size: 3350 900
BsaAI yAC'GTr
Cuts at: 1603 1603
Size: 4250
BsaBI GATnn'nnATC
Cuts at: 6 1764 3801 6
Size: 1758 2037 455
Fragments arranged by size:
2037 1758 455
BsaHI Gr'CG_yC
Cuts at: 164 1681 1929 2631 4127 164
Size: 1517 248 702 1496 287
Fragments arranged by size:
1517 1496 702 287 248
BsaWI w'CCGG_w
Cuts at: 1439 1960 2638 2912 3059 3470 1439
Size: 521 678 274 147 411 2219
Fragments arranged by size:
2219 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 131 253 1675 2433 3010 3506 131
Size: 122 1422 758 577 496 875
Fragments arranged by size:
1422 875 758 577 496 122
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 787 787
Size: 4250
BsiEI CG_ry'CG
Cuts at: 104 1792 1838 3046 104
Size: 1688 46 1208 1308
Fragments arranged by size:
1688 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1264 2042 2232 3024 1264
Size: 778 190 792 2490
Fragments arranged by size:
2490 792 778 190
BsmI GAATG_Cn'
Cuts at: 690 690
Size: 4250
Bsp1286I G_dGCh'C
Cuts at: 348 411 957 979 1264 1875 1968 2042 2232
Size: 63 546 22 285 611 93 74 190
Cuts at: 2232 2294 3024 348
Size: 62 730 1574
Fragments arranged by size:
1574 730 611 546 285 190 93 74
63 62 22
BspEI T'CCGG_A
Cuts at: 3470 3470
Size: 4250
BspHI T'CATG_A
Cuts at: 3426 3426
Size: 4250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1300 1514 1816 2197 1300
Size: 214 302 381 3353
Fragments arranged by size:
3353 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2542 2596 4080 4245 50
Size: 2492 54 1484 165 55
Fragments arranged by size:
2492 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 315 2162 315
Size: 1847 2403
BsrFI r'CCGG_y
Cuts at: 2248 2429 2638 2248
Size: 181 209 3860
Fragments arranged by size:
3860 209 181
BssSI C'ACGA_G
Cuts at: 2521 2879 2521
Size: 358 3892
BstAPI GCAn_nnn'nTGC
Cuts at: 1627 1627
Size: 4250
BstBI TT'CG_AA
Cuts at: 2611 2611
Size: 4250
BstEII G'GTnAC_C
Cuts at: 135 135
Size: 4250
BstYI r'GATC_y
Cuts at: 1 232 633 1275 1742 1765 2100 2346 3358
Size: 231 401 642 467 23 335 246 1012
Cuts at: 3358 3444 3456 3802 1
Size: 86 12 346 449
Fragments arranged by size:
1012 642 467 449 401 346 335 246
231 86 23 12
BstZ17I GTA'TAC
Cuts at: 714 714
Size: 4250
BtgI C'CryG_G
Cuts at: 107 107
Size: 4250
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2200 2381 2544 2200
Size: 181 163 3906
Fragments arranged by size:
3906 181 163
BtsI GCAGTG_nn'
Cuts at: 1493 1493
Size: 4250
DraI TTT'AAA
Cuts at: 1118 1249 1118
Size: 131 4119
DrdI GACnn_nn'nnGTC
Cuts at: 1956 2814 1956
Size: 858 3392
EaeI y'GGCC_r
Cuts at: 1205 1835 2009 2400 2427 3772 1205
Size: 630 174 391 27 1345 1683
Fragments arranged by size:
1683 1345 630 391 174 27
EagI C'GGCC_G
Cuts at: 1835 1835
Size: 4250
EarI CTCTTCn'nnn_
Cuts at: 2273 2483 2273
Size: 210 4040
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2458 2768 2914 4060 2458
Size: 310 146 1146 2648
Fragments arranged by size:
2648 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1262 1262
Size: 4250
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 380 874 2075 2507 3254 3522 380
Size: 494 1201 432 747 268 1108
Fragments arranged by size:
1201 1108 747 494 432 268
EcoO109I rG'GnC_Cy
Cuts at: 953 1389 953
Size: 436 3814
EcoRI G'AATT_C
Cuts at: 1254 1254
Size: 4250
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1030 1120 2269 1030
Size: 90 1149 3011
Fragments arranged by size:
3011 1149 90
FspI TGC'GCA
Cuts at: 2031 2031
Size: 4250
HaeII r_GCGC'y
Cuts at: 167 1684 1932 2954 3933 167
Size: 1517 248 1022 979 484
Fragments arranged by size:
1517 1022 979 484 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 217 249 2092 2122 2124 2154 217
Size: 32 1843 30 2 30 2313
Fragments arranged by size:
2313 1843 32 30 30 2
HincII GTy'rAC
Cuts at: 1289 1289
Size: 4250
KasI G'GCGC_C
Cuts at: 163 1680 1928 163
Size: 1517 248 2485
Fragments arranged by size:
2485 1517 248
KpnI G_GTAC'C
Cuts at: 336 1270 336
Size: 934 3316
MfeI C'AATT_G
Cuts at: 605 605
Size: 4250
MluI A'CGCG_T
Cuts at: 3683 3683
Size: 4250
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1173 2921 3105 1173
Size: 1748 184 2318
Fragments arranged by size:
2318 1748 184
MscI TGG'CCA
Cuts at: 1207 2011 1207
Size: 804 3446
MslI CAynn'nnrTG
Cuts at: 2239 2366 2239
Size: 127 4123
MspA1I CmG'CkG
Cuts at: 868 1361 1675 2035 3048 3293 868
Size: 493 314 360 1013 245 1825
Fragments arranged by size:
1825 1013 493 360 314 245
NaeI GCC'GGC
Cuts at: 2431 2431
Size: 4250
NarI GG'CG_CC
Cuts at: 164 1681 1929 164
Size: 1517 248 2485
Fragments arranged by size:
2485 1517 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4250
NdeI CA'TA_TG
Cuts at: 1223 1223
Size: 4250
NgoMIV G'CCGG_C
Cuts at: 2429 2429
Size: 4250
NsiI A_TGCA'T
Cuts at: 567 567
Size: 4250
NspI r_CATG'y
Cuts at: 828 1303 1803 2710 828
Size: 475 500 907 2368
Fragments arranged by size:
2368 907 500 475
PciI A'CATG_T
Cuts at: 2706 2706
Size: 4250
PmeI GTTT'AAAC
Cuts at: 1249 1249
Size: 4250
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3389 3421 3389
Size: 32 4218
PspOMI G'GGCC_C
Cuts at: 953 953
Size: 4250
PstI C_TGCA'G
Cuts at: 1297 1982 1297
Size: 685 3565
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4250
PvuII CAG'CTG
Cuts at: 1675 2035 1675
Size: 360 3890
RsrII CG'GwC_CG
Cuts at: 2445 2445
Size: 4250
SacI G_AGCT'C
Cuts at: 1264 1264
Size: 4250
SalI G'TCGA_C
Cuts at: 1287 1287
Size: 4250
SapI GCTCTTCn'nnn_
Cuts at: 2273 2483 2273
Size: 210 4040
SbfI CC_TGCA'GG
Cuts at: 1297 1297
Size: 4250
ScaI AGT'ACT
Cuts at: 3767 3767
Size: 4250
SfcI C'TryA_G
Cuts at: 33 1293 1978 2971 3162 33
Size: 1260 685 993 191 1121
Fragments arranged by size:
1260 1121 993 685 191
SfoI GGC'GCC
Cuts at: 165 1682 1930 165
Size: 1517 248 2485
Fragments arranged by size:
2485 1517 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4250
SmaI CCC'GGG
Cuts at: 406 1272 406
Size: 866 3384
SmlI C'TyrA_G
Cuts at: 467 675 1405 2320 2812 3074 467
Size: 208 730 915 492 262 1643
Fragments arranged by size:
1643 915 730 492 262 208
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4250
SphI G_CATG'C
Cuts at: 1303 1803 1303
Size: 500 3750
StuI AGG'CCT
Cuts at: 146 146
Size: 4250
StyI C'CwwG_G
Cuts at: 107 340 1384 1738 107
Size: 233 1044 354 2619
Fragments arranged by size:
2619 1044 354 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 123 583 1833 1966 2425 2634 123
Size: 460 1250 133 459 209 1739
Fragments arranged by size:
1739 1250 460 459 209 133
TatI w'GTAC_w
Cuts at: 3765 3765
Size: 4250
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3767 3799 3767
Size: 32 4218
Tth111I GACn'n_nGTC
Cuts at: 2047 2047
Size: 4250
XbaI T'CTAG_A
Cuts at: 1281 1281
Size: 4250
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1745 1745
Size: 4250
XmaI C'CCGG_G
Cuts at: 404 1270 404
Size: 866 3384
XmnI GAAnn'nnTTC
Cuts at: 1010 1087 1010
Size: 77 4173
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AscI AvrII
BaeI BbsI BcgI BglI BmtI BpmI BsaXI BseRI
BsiWI BsmBI BsrGI BssHII BstXI Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI NheI
NotI NruI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI