Restriction map for
pF1KB8805
(Circular) (Six-base) MAPSORT of: pF1KB8805.seq Check: 8148 from: 1 to: 4166
pF1KB8805 4166 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 806 1121 1204 806
Size: 315 83 3768
Fragments arranged by size:
3768 315 83
Acc65I G'GTAC_C
Cuts at: 1182 1182
Size: 4166
AclI AA'CG_TT
Cuts at: 4006 4006
Size: 4166
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1002 1991 2423 3170 3438 1002
Size: 989 432 747 268 1730
Fragments arranged by size:
1730 989 747 432 268
AflIII A'CryG_T
Cuts at: 1518 2622 3599 1518
Size: 1104 977 2085
Fragments arranged by size:
2085 1104 977
AgeI A'CCGG_T
Cuts at: 325 2554 325
Size: 2229 1937
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 229 229
Size: 4166
AlwNI CAG_nnn'CTG
Cuts at: 192 1471 3038 192
Size: 1279 1567 1320
Fragments arranged by size:
1567 1320 1279
ApaI G_GGCC'C
Cuts at: 427 662 427
Size: 235 3931
ApaLI G'TGCA_C
Cuts at: 2936 2936
Size: 4166
ApoI r'AATT_y
Cuts at: 1170 3519 3775 1170
Size: 2349 256 1561
Fragments arranged by size:
2349 1561 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4166
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4166
AvaI C'yCGr_G
Cuts at: 128 318 409 1186 128
Size: 190 91 777 3108
Fragments arranged by size:
3108 777 190 91
BamHI G'GATC_C
Cuts at: 1191 1191
Size: 4166
BanI G'GyrC_C
Cuts at: 133 322 595 638 672 777 794 949 1182
Size: 189 273 43 34 105 17 155 233
Cuts at: 1182 1596 1844 1879 133
Size: 414 248 35 2420
Fragments arranged by size:
2420 414 273 248 233 189 155 105
43 35 34 17
BanII G_rGCy'C
Cuts at: 416 427 662 815 1180 2210 416
Size: 11 235 153 365 1030 2372
Fragments arranged by size:
2372 1030 365 235 153 11
BbeI G_GCGC'C
Cuts at: 137 599 781 798 953 1600 1848 137
Size: 462 182 17 155 647 248 2455
Fragments arranged by size:
2455 647 462 248 182 155 17
BciVI GTATCCnnnnn_n'
Cuts at: 2064 2825 4166 2064
Size: 761 1341 2064
Fragments arranged by size:
2064 1341 761
BclI T'GATC_A
Cuts at: 1686 1686
Size: 4166
BglI GCCn_nnn'nGGC
Cuts at: 653 657 792 653
Size: 4 135 4027
Fragments arranged by size:
4027 135 4
BglII A'GATC_T
Cuts at: 1681 3718 1681
Size: 2037 2129
BlpI GC'TnA_GC
Cuts at: 505 814 1278 505
Size: 309 464 3393
Fragments arranged by size:
3393 464 309
Bme1580I G_kGCm'C
Cuts at: 325 427 662 775 1791 1884 2940 325
Size: 102 235 113 1016 93 1056 1551
Fragments arranged by size:
1551 1056 1016 235 113 102 93
BmgBI CAC'GTC
Cuts at: 717 717
Size: 4166
BmrI ACTGGGnnnn_n'
Cuts at: 421 1378 1459 1794 3938 421
Size: 957 81 335 2144 649
Fragments arranged by size:
2144 957 649 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2533 2533
Size: 4166
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 135 316 135
Size: 181 3985
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1342 2221 2713 3011 1342
Size: 879 492 298 2497
Fragments arranged by size:
2497 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3870 3870
Size: 4166
BsaAI yAC'GTr
Cuts at: 1519 1519
Size: 4166
BsaBI GATnn'nnATC
Cuts at: 6 1680 3717 6
Size: 1674 2037 455
Fragments arranged by size:
2037 1674 455
BsaHI Gr'CG_yC
Cuts at: 134 596 778 795 950 1597 1845 2547 4043
Size: 462 182 17 155 647 248 702 1496
Cuts at: 4043 134
Size: 257
Fragments arranged by size:
1496 702 647 462 257 248 182 155
17
BsaWI w'CCGG_w
Cuts at: 325 1355 1876 2554 2828 2975 3386 325
Size: 1030 521 678 274 147 411 1105
Fragments arranged by size:
1105 1030 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 259 283 289 313 259
Size: 24 6 24 4112
Fragments arranged by size:
4112 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 264 1000 1171 264
Size: 736 171 3259
Fragments arranged by size:
3259 736 171
BseYI C'CCAG_C
Cuts at: 517 1591 2349 2926 3422 517
Size: 1074 758 577 496 1261
Fragments arranged by size:
1261 1074 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 572 684 935 1708 1754 2962 104
Size: 468 112 251 773 46 1208 1308
Fragments arranged by size:
1308 1208 773 468 251 112 46
BsiHKAI G_wGCw'C
Cuts at: 416 815 1180 1958 2148 2940 416
Size: 399 365 778 190 792 1642
Fragments arranged by size:
1642 792 778 399 365 190
Bsp1286I G_dGCh'C
Cuts at: 325 416 427 662 775 815 1180 1791 1884
Size: 91 11 235 113 40 365 611 93
Cuts at: 1884 1958 2148 2210 2940 325
Size: 74 190 62 730 1551
Fragments arranged by size:
1551 730 611 365 235 190 113 93
91 74 62 40 11
BspEI T'CCGG_A
Cuts at: 3386 3386
Size: 4166
BspHI T'CATG_A
Cuts at: 3342 3342
Size: 4166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1216 1430 1732 2113 1216
Size: 214 302 381 3269
Fragments arranged by size:
3269 381 302 214
BsrBI CCG'CTC
Cuts at: 50 219 509 678 686 2458 2512 3996 4161
Size: 169 290 169 8 1772 54 1484 165
Cuts at: 4161 50
Size: 55
Fragments arranged by size:
1772 1484 290 169 169 165 55 54
8
BsrDI GCAATG_nn'
Cuts at: 2078 2078
Size: 4166
BsrFI r'CCGG_y
Cuts at: 325 470 571 797 835 1099 2164 2345 2554
Size: 145 101 226 38 264 1065 181 209
Cuts at: 2554 325
Size: 1937
Fragments arranged by size:
1937 1065 264 226 209 181 145 101
38
BssHII G'CGCG_C
Cuts at: 521 907 987 521
Size: 386 80 3700
Fragments arranged by size:
3700 386 80
BssSI C'ACGA_G
Cuts at: 384 1029 2437 2795 384
Size: 645 1408 358 1755
Fragments arranged by size:
1755 1408 645 358
BstAPI GCAn_nnn'nTGC
Cuts at: 192 1543 192
Size: 1351 2815
BstBI TT'CG_AA
Cuts at: 2527 2527
Size: 4166
BstYI r'GATC_y
Cuts at: 1 1191 1658 1681 2016 2262 3274 3360 3372
Size: 1190 467 23 335 246 1012 86 12
Cuts at: 3372 3718 1
Size: 346 449
Fragments arranged by size:
1190 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 122 347 943 122
Size: 225 596 3345
Fragments arranged by size:
3345 596 225
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 192 567 585 678 1047 2116 2297 2460 192
Size: 375 18 93 369 1069 181 163 1898
Fragments arranged by size:
1898 1069 375 369 181 163 93 18
BtsI GCAGTG_nn'
Cuts at: 1409 1409
Size: 4166
DraI TTT'AAA
Cuts at: 1165 1165
Size: 4166
DrdI GACnn_nn'nnGTC
Cuts at: 715 1872 2730 715
Size: 1157 858 2151
Fragments arranged by size:
2151 1157 858
EaeI y'GGCC_r
Cuts at: 200 354 569 799 932 999 1751 1925 2316
Size: 154 215 230 133 67 752 174 391
Cuts at: 2316 2343 3688 200
Size: 27 1345 678
Fragments arranged by size:
1345 752 678 391 230 215 174 154
133 67 27
EagI C'GGCC_G
Cuts at: 569 932 1751 569
Size: 363 819 2984
Fragments arranged by size:
2984 819 363
EarI CTCTTCn'nnn_
Cuts at: 226 421 619 2189 2399 226
Size: 195 198 1570 210 1993
Fragments arranged by size:
1993 1570 210 198 195
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 904 2374 2684 2830 3976 904
Size: 1470 310 146 1146 1094
Fragments arranged by size:
1470 1146 1094 310 146
EcoICRI GAG'CTC
Cuts at: 414 813 1178 414
Size: 399 365 3402
Fragments arranged by size:
3402 399 365
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 135 316 1002 1991 2423 3170 3438 135
Size: 181 686 989 432 747 268 863
Fragments arranged by size:
989 863 747 686 432 268 181
EcoO109I rG'GnC_Cy
Cuts at: 423 659 1305 423
Size: 236 646 3284
Fragments arranged by size:
3284 646 236
EcoRI G'AATT_C
Cuts at: 1170 1170
Size: 4166
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1068 2185 1068
Size: 1117 3049
FseI GG_CCGG'CC
Cuts at: 575 575
Size: 4166
FspI TGC'GCA
Cuts at: 871 1947 871
Size: 1076 3090
FspAI rTGC'GCAy
Cuts at: 871 871
Size: 4166
HaeII r_GCGC'y
Cuts at: 122 137 174 370 477 599 648 781 798
Size: 15 37 196 107 122 49 133 17
Cuts at: 798 883 953 1600 1848 2870 3849 122
Size: 85 70 647 248 1022 979 439
Fragments arranged by size:
1022 979 647 439 248 196 133 122
107 85 70 49 37 17 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 613 645 2008 2038 2040 2070 613
Size: 32 1363 30 2 30 2709
Fragments arranged by size:
2709 1363 32 30 30 2
HincII GTy'rAC
Cuts at: 540 744 963 1205 540
Size: 204 219 242 3501
Fragments arranged by size:
3501 242 219 204
KasI G'GCGC_C
Cuts at: 133 595 777 794 949 1596 1844 133
Size: 462 182 17 155 647 248 2455
Fragments arranged by size:
2455 647 462 248 182 155 17
KpnI G_GTAC'C
Cuts at: 1186 1186
Size: 4166
MluI A'CGCG_T
Cuts at: 3599 3599
Size: 4166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 302 2837 3021 302
Size: 2535 184 1447
Fragments arranged by size:
2535 1447 184
MscI TGG'CCA
Cuts at: 356 1927 356
Size: 1571 2595
MslI CAynn'nnrTG
Cuts at: 2155 2282 2155
Size: 127 4039
MspA1I CmG'CkG
Cuts at: 192 349 504 738 945 1277 1591 1951 2964
Size: 157 155 234 207 332 314 360 1013
Cuts at: 2964 3209 192
Size: 245 1149
Fragments arranged by size:
1149 1013 360 332 314 245 234 207
157 155
NaeI GCC'GGC
Cuts at: 573 799 2347 573
Size: 226 1548 2392
Fragments arranged by size:
2392 1548 226
NarI GG'CG_CC
Cuts at: 134 596 778 795 950 1597 1845 134
Size: 462 182 17 155 647 248 2455
Fragments arranged by size:
2455 647 462 248 182 155 17
NgoMIV G'CCGG_C
Cuts at: 571 797 2345 571
Size: 226 1548 2392
Fragments arranged by size:
2392 1548 226
NspI r_CATG'y
Cuts at: 1219 1719 2626 1219
Size: 500 907 2759
Fragments arranged by size:
2759 907 500
PciI A'CATG_T
Cuts at: 2622 2622
Size: 4166
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 4166
PmeI GTTT'AAAC
Cuts at: 1165 1165
Size: 4166
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3305 3337 3305
Size: 32 4134
PspOMI G'GGCC_C
Cuts at: 423 658 423
Size: 235 3931
PstI C_TGCA'G
Cuts at: 1213 1898 1213
Size: 685 3481
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4166
PvuII CAG'CTG
Cuts at: 504 1591 1951 504
Size: 1087 360 2719
Fragments arranged by size:
2719 1087 360
RsrII CG'GwC_CG
Cuts at: 2361 2361
Size: 4166
SacI G_AGCT'C
Cuts at: 416 815 1180 416
Size: 399 365 3402
Fragments arranged by size:
3402 399 365
SacII CC_GC'GG
Cuts at: 350 946 350
Size: 596 3570
SalI G'TCGA_C
Cuts at: 1203 1203
Size: 4166
SapI GCTCTTCn'nnn_
Cuts at: 226 421 2189 2399 226
Size: 195 1768 210 1993
Fragments arranged by size:
1993 1768 210 195
SbfI CC_TGCA'GG
Cuts at: 1213 1213
Size: 4166
ScaI AGT'ACT
Cuts at: 3683 3683
Size: 4166
SexAI A'CCwGG_T
Cuts at: 1070 1070
Size: 4166
SfcI C'TryA_G
Cuts at: 33 1209 1894 2887 3078 33
Size: 1176 685 993 191 1121
Fragments arranged by size:
1176 1121 993 685 191
SfoI GGC'GCC
Cuts at: 135 597 779 796 951 1598 1846 135
Size: 462 182 17 155 647 248 2455
Fragments arranged by size:
2455 647 462 248 182 155 17
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4166
SmaI CCC'GGG
Cuts at: 130 320 1188 130
Size: 190 868 3108
Fragments arranged by size:
3108 868 190
SmlI C'TyrA_G
Cuts at: 1321 2236 2728 2990 1321
Size: 915 492 262 2497
Fragments arranged by size:
2497 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4166
SphI G_CATG'C
Cuts at: 1219 1719 1219
Size: 500 3666
StyI C'CwwG_G
Cuts at: 1300 1654 1300
Size: 354 3812
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 721 1027 1749 1882 2341 2550 721
Size: 306 722 133 459 209 2337
Fragments arranged by size:
2337 722 459 306 209 133
TatI w'GTAC_w
Cuts at: 3681 3681
Size: 4166
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3683 3715 3683
Size: 32 4134
Tth111I GACn'n_nGTC
Cuts at: 1963 1963
Size: 4166
XbaI T'CTAG_A
Cuts at: 1197 1197
Size: 4166
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1661 1661
Size: 4166
XmaI C'CCGG_G
Cuts at: 128 318 1186 128
Size: 190 868 3108
Fragments arranged by size:
3108 868 190
XmnI GAAnn'nnTTC
Cuts at: 1051 1051
Size: 4166
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FseI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI Bpu10I
BsgI BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI