Restriction map for
pF1KB8799
(Circular) (Six-base) MAPSORT of: pF1KB8799.seq Check: 2188 from: 1 to: 4127
pF1KB8799 4127 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1165 1165
Size: 4127
Acc65I G'GTAC_C
Cuts at: 1143 1143
Size: 4127
AclI AA'CG_TT
Cuts at: 3967 3967
Size: 4127
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 107 619 865 1952 2384 3131 3399 107
Size: 512 246 1087 432 747 268 835
Fragments arranged by size:
1087 835 747 512 432 268 246
AflIII A'CryG_T
Cuts at: 1479 2583 3560 1479
Size: 1104 977 2046
Fragments arranged by size:
2046 1104 977
AgeI A'CCGG_T
Cuts at: 2515 2515
Size: 4127
AlwNI CAG_nnn'CTG
Cuts at: 860 1432 2999 860
Size: 572 1567 1988
Fragments arranged by size:
1988 1567 572
ApaLI G'TGCA_C
Cuts at: 2897 2897
Size: 4127
ApoI r'AATT_y
Cuts at: 264 474 1131 3480 3736 264
Size: 210 657 2349 256 655
Fragments arranged by size:
2349 657 655 256 210
AseI AT'TA_AT
Cuts at: 20 20
Size: 4127
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4127
AvaI C'yCGr_G
Cuts at: 1147 1147
Size: 4127
BamHI G'GATC_C
Cuts at: 1032 1152 1032
Size: 120 4007
BanI G'GyrC_C
Cuts at: 1143 1557 1805 1840 1143
Size: 414 248 35 3430
Fragments arranged by size:
3430 414 248 35
BanII G_rGCy'C
Cuts at: 1141 2171 1141
Size: 1030 3097
BbeI G_GCGC'C
Cuts at: 1561 1809 1561
Size: 248 3879
BciVI GTATCCnnnnn_n'
Cuts at: 2025 2786 4127 2025
Size: 761 1341 2025
Fragments arranged by size:
2025 1341 761
BclI T'GATC_A
Cuts at: 197 1647 197
Size: 1450 2677
BglII A'GATC_T
Cuts at: 811 1642 3679 811
Size: 831 2037 1259
Fragments arranged by size:
2037 1259 831
BlpI GC'TnA_GC
Cuts at: 1239 1239
Size: 4127
Bme1580I G_kGCm'C
Cuts at: 1752 1845 2901 1752
Size: 93 1056 2978
Fragments arranged by size:
2978 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1339 1420 1755 3899 1339
Size: 81 335 2144 1567
Fragments arranged by size:
2144 1567 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 150 2494 150
Size: 2344 1783
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 780 780
Size: 4127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1105 1303 2182 2674 2972 1105
Size: 198 879 492 298 2260
Fragments arranged by size:
2260 879 492 298 198
BsaI GGTCTCn'nnnn_
Cuts at: 569 3831 569
Size: 3262 865
BsaAI yAC'GTr
Cuts at: 1480 1480
Size: 4127
BsaBI GATnn'nnATC
Cuts at: 6 1641 3678 6
Size: 1635 2037 455
Fragments arranged by size:
2037 1635 455
BsaHI Gr'CG_yC
Cuts at: 1558 1806 2508 4004 1558
Size: 248 702 1496 1681
Fragments arranged by size:
1681 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1316 1837 2515 2789 2936 3347 1316
Size: 521 678 274 147 411 2096
Fragments arranged by size:
2096 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 579 1552 2310 2887 3383 579
Size: 973 758 577 496 1323
Fragments arranged by size:
1323 973 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1669 1715 2923 104
Size: 1565 46 1208 1308
Fragments arranged by size:
1565 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1141 1919 2109 2901 1141
Size: 778 190 792 2367
Fragments arranged by size:
2367 792 778 190
BsmI GAATG_Cn'
Cuts at: 295 615 644 295
Size: 320 29 3778
Fragments arranged by size:
3778 320 29
Bsp1286I G_dGCh'C
Cuts at: 1141 1752 1845 1919 2109 2171 2901 1141
Size: 611 93 74 190 62 730 2367
Fragments arranged by size:
2367 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3347 3347
Size: 4127
BspHI T'CATG_A
Cuts at: 3303 3303
Size: 4127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1177 1391 1693 2074 1177
Size: 214 302 381 3230
Fragments arranged by size:
3230 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2419 2473 3957 4122 50
Size: 2369 54 1484 165 55
Fragments arranged by size:
2369 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 968 2039 968
Size: 1071 3056
BsrFI r'CCGG_y
Cuts at: 2125 2306 2515 2125
Size: 181 209 3737
Fragments arranged by size:
3737 209 181
BssSI C'ACGA_G
Cuts at: 2398 2756 2398
Size: 358 3769
BstAPI GCAn_nnn'nTGC
Cuts at: 610 1504 610
Size: 894 3233
BstBI TT'CG_AA
Cuts at: 2488 2488
Size: 4127
BstEII G'GTnAC_C
Cuts at: 863 863
Size: 4127
BstYI r'GATC_y
Cuts at: 1 811 1032 1152 1619 1642 1977 2223 3235
Size: 810 221 120 467 23 335 246 1012
Cuts at: 3235 3321 3333 3679 1
Size: 86 12 346 449
Fragments arranged by size:
1012 810 467 449 346 335 246 221
120 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2077 2258 2421 2077
Size: 181 163 3783
Fragments arranged by size:
3783 181 163
BtsI GCAGTG_nn'
Cuts at: 1370 1370
Size: 4127
DraI TTT'AAA
Cuts at: 1010 1126 1010
Size: 116 4011
DrdI GACnn_nn'nnGTC
Cuts at: 1833 2691 1833
Size: 858 3269
EaeI y'GGCC_r
Cuts at: 1712 1886 2277 2304 3649 1712
Size: 174 391 27 1345 2190
Fragments arranged by size:
2190 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1712 1712
Size: 4127
EarI CTCTTCn'nnn_
Cuts at: 126 2150 2360 126
Size: 2024 210 1893
Fragments arranged by size:
2024 1893 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2335 2645 2791 3937 2335
Size: 310 146 1146 2525
Fragments arranged by size:
2525 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1139 1139
Size: 4127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 107 619 780 865 1952 2384 3131 3399 107
Size: 512 161 85 1087 432 747 268 835
Fragments arranged by size:
1087 835 747 512 432 268 161 85
EcoO109I rG'GnC_Cy
Cuts at: 1266 1266
Size: 4127
EcoRI G'AATT_C
Cuts at: 1131 1131
Size: 4127
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1074 2146 1074
Size: 1072 3055
FspI TGC'GCA
Cuts at: 1908 1908
Size: 4127
HaeII r_GCGC'y
Cuts at: 1561 1809 2831 3810 1561
Size: 248 1022 979 1878
Fragments arranged by size:
1878 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1969 1999 2001 2031 1969
Size: 30 2 30 4065
Fragments arranged by size:
4065 30 30 2
HincII GTy'rAC
Cuts at: 180 1044 1166 180
Size: 864 122 3141
Fragments arranged by size:
3141 864 122
HindIII A'AGCT_T
Cuts at: 493 1000 493
Size: 507 3620
HpaI GTT'AAC
Cuts at: 180 180
Size: 4127
KasI G'GCGC_C
Cuts at: 1557 1805 1557
Size: 248 3879
KpnI G_GTAC'C
Cuts at: 1147 1147
Size: 4127
MluI A'CGCG_T
Cuts at: 3560 3560
Size: 4127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2798 2982 2798
Size: 184 3943
MscI TGG'CCA
Cuts at: 1888 1888
Size: 4127
MslI CAynn'nnrTG
Cuts at: 504 676 2116 2243 504
Size: 172 1440 127 2388
Fragments arranged by size:
2388 1440 172 127
MspA1I CmG'CkG
Cuts at: 1238 1552 1912 2925 3170 1238
Size: 314 360 1013 245 2195
Fragments arranged by size:
2195 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2308 2308
Size: 4127
NarI GG'CG_CC
Cuts at: 1558 1806 1558
Size: 248 3879
NgoMIV G'CCGG_C
Cuts at: 2306 2306
Size: 4127
NspI r_CATG'y
Cuts at: 1180 1680 2587 1180
Size: 500 907 2720
Fragments arranged by size:
2720 907 500
PciI A'CATG_T
Cuts at: 2583 2583
Size: 4127
PmeI GTTT'AAAC
Cuts at: 1126 1126
Size: 4127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3266 3298 3266
Size: 32 4095
PstI C_TGCA'G
Cuts at: 1174 1859 1174
Size: 685 3442
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4127
PvuII CAG'CTG
Cuts at: 1552 1912 1552
Size: 360 3767
RsrII CG'GwC_CG
Cuts at: 2322 2322
Size: 4127
SacI G_AGCT'C
Cuts at: 1141 1141
Size: 4127
SalI G'TCGA_C
Cuts at: 1164 1164
Size: 4127
SapI GCTCTTCn'nnn_
Cuts at: 2150 2360 2150
Size: 210 3917
SbfI CC_TGCA'GG
Cuts at: 1174 1174
Size: 4127
ScaI AGT'ACT
Cuts at: 3644 3644
Size: 4127
SexAI A'CCwGG_T
Cuts at: 859 859
Size: 4127
SfcI C'TryA_G
Cuts at: 33 974 1170 1855 2848 3039 33
Size: 941 196 685 993 191 1121
Fragments arranged by size:
1121 993 941 685 196 191
SfoI GGC'GCC
Cuts at: 1559 1807 1559
Size: 248 3879
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4127
SmaI CCC'GGG
Cuts at: 1149 1149
Size: 4127
SmlI C'TyrA_G
Cuts at: 1084 1282 2197 2689 2951 1084
Size: 198 915 492 262 2260
Fragments arranged by size:
2260 915 492 262 198
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4127
SphI G_CATG'C
Cuts at: 1180 1680 1180
Size: 500 3627
SspI AAT'ATT
Cuts at: 215 277 824 936 215
Size: 62 547 112 3406
Fragments arranged by size:
3406 547 112 62
StyI C'CwwG_G
Cuts at: 1261 1615 1261
Size: 354 3773
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1710 1843 2302 2511 1710
Size: 133 459 209 3326
Fragments arranged by size:
3326 459 209 133
TatI w'GTAC_w
Cuts at: 354 873 3642 354
Size: 519 2769 839
Fragments arranged by size:
2769 839 519
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3644 3676 3644
Size: 32 4095
Tth111I GACn'n_nGTC
Cuts at: 1924 1924
Size: 4127
XbaI T'CTAG_A
Cuts at: 1158 1158
Size: 4127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1043 1622 1043
Size: 579 3548
XmaI C'CCGG_G
Cuts at: 1147 1147
Size: 4127
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI
BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI