Restriction map for
pF1KB8708
(Circular) (Six-base) MAPSORT of: pF1KB8708.seq Check: 1803 from: 1 to: 5036
pF1KB8708 5036 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 586 586
Size: 5036
AccI GT'mk_AC
Cuts at: 2074 2074
Size: 5036
Acc65I G'GTAC_C
Cuts at: 1048 2052 1048
Size: 1004 4032
AclI AA'CG_TT
Cuts at: 4876 4876
Size: 5036
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1147 1715 2861 3293 4040 4308 1147
Size: 568 1146 432 747 268 1875
Fragments arranged by size:
1875 1146 747 568 432 268
AfeI AGC'GCT
Cuts at: 1073 1073
Size: 5036
AflIII A'CryG_T
Cuts at: 693 2388 3492 4469 693
Size: 1695 1104 977 1260
Fragments arranged by size:
1695 1260 1104 977
AgeI A'CCGG_T
Cuts at: 3424 3424
Size: 5036
AleI CACnn'nnGTG
Cuts at: 797 797
Size: 5036
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 680 712 680
Size: 32 5004
AlwNI CAG_nnn'CTG
Cuts at: 1158 1284 2341 3908 1158
Size: 126 1057 1567 2286
Fragments arranged by size:
2286 1567 1057 126
ApaLI G'TGCA_C
Cuts at: 3806 3806
Size: 5036
ApoI r'AATT_y
Cuts at: 343 2040 4389 4645 343
Size: 1697 2349 256 734
Fragments arranged by size:
2349 1697 734 256
AseI AT'TA_AT
Cuts at: 20 398 20
Size: 378 4658
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5036
AvaI C'yCGr_G
Cuts at: 1420 2056 1420
Size: 636 4400
BamHI G'GATC_C
Cuts at: 2061 2061
Size: 5036
BanI G'GyrC_C
Cuts at: 148 1048 2052 2466 2714 2749 148
Size: 900 1004 414 248 35 2435
Fragments arranged by size:
2435 1004 900 414 248 35
BanII G_rGCy'C
Cuts at: 641 1014 1132 1844 2050 3080 641
Size: 373 118 712 206 1030 2597
Fragments arranged by size:
2597 1030 712 373 206 118
BbeI G_GCGC'C
Cuts at: 2470 2718 2470
Size: 248 4788
BbvCI CC'TCA_GC
Cuts at: 506 506
Size: 5036
BciVI GTATCCnnnnn_n'
Cuts at: 2934 3695 5036 2934
Size: 761 1341 2934
Fragments arranged by size:
2934 1341 761
BclI T'GATC_A
Cuts at: 1890 2556 1890
Size: 666 4370
BglI GCCn_nnn'nGGC
Cuts at: 1995 1995
Size: 5036
BglII A'GATC_T
Cuts at: 2551 4588 2551
Size: 2037 2999
BlpI GC'TnA_GC
Cuts at: 2148 2148
Size: 5036
Bme1580I G_kGCm'C
Cuts at: 151 2661 2754 3810 151
Size: 2510 93 1056 1377
Fragments arranged by size:
2510 1377 1056 93
BmgBI CAC'GTC
Cuts at: 1183 1299 1183
Size: 116 4920
BmrI ACTGGGnnnn_n'
Cuts at: 1970 2248 2329 2664 4808 1970
Size: 278 81 335 2144 2198
Fragments arranged by size:
2198 2144 335 278 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 486 3403 486
Size: 2917 2119
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1075 1342 1493 1876 1075
Size: 267 151 383 4235
Fragments arranged by size:
4235 383 267 151
Bpu10I CC'TnA_GC
Cuts at: 506 1642 506
Size: 1136 3900
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2212 3091 3583 3881 2212
Size: 879 492 298 3367
Fragments arranged by size:
3367 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4740 4740
Size: 5036
BsaAI yAC'GTr
Cuts at: 237 2389 237
Size: 2152 2884
BsaBI GATnn'nnATC
Cuts at: 6 1618 2550 4587 6
Size: 1612 932 2037 455
Fragments arranged by size:
2037 1612 932 455
BsaHI Gr'CG_yC
Cuts at: 2467 2715 3417 4913 2467
Size: 248 702 1496 2590
Fragments arranged by size:
2590 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2225 2746 3424 3698 3845 4256 2225
Size: 521 678 274 147 411 3005
Fragments arranged by size:
3005 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1903 1933 1903
Size: 30 5006
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 856 1666 856
Size: 810 4226
BseYI C'CCAG_C
Cuts at: 229 781 1330 1479 2461 3219 3796 4292 229
Size: 552 549 149 982 758 577 496 973
Fragments arranged by size:
982 973 758 577 552 549 496 149
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1052 1171 1052
Size: 119 4917
BsiEI CG_ry'CG
Cuts at: 104 2578 2624 3832 104
Size: 2474 46 1208 1308
Fragments arranged by size:
2474 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1014 1597 1844 2050 2828 3018 3810 1014
Size: 583 247 206 778 190 792 2240
Fragments arranged by size:
2240 792 778 583 247 206 190
BsmI GAATG_Cn'
Cuts at: 1737 1737
Size: 5036
BsmBI CGTCTCn'nnnn_
Cuts at: 1318 1318
Size: 5036
Bsp1286I G_dGCh'C
Cuts at: 151 641 1014 1132 1597 1844 2050 2661 2754
Size: 490 373 118 465 247 206 611 93
Cuts at: 2754 2828 3018 3080 3810 151
Size: 74 190 62 730 1377
Fragments arranged by size:
1377 730 611 490 465 373 247 206
190 118 93 74 62
BspEI T'CCGG_A
Cuts at: 4256 4256
Size: 5036
BspHI T'CATG_A
Cuts at: 4212 4212
Size: 5036
BspMI ACCTGCnnnn'nnnn_
Cuts at: 586 1292 1484 2086 2300 2602 2983 586
Size: 706 192 602 214 302 381 2639
Fragments arranged by size:
2639 706 602 381 302 214 192
BsrBI CCG'CTC
Cuts at: 50 258 3328 3382 4866 5031 50
Size: 208 3070 54 1484 165 55
Fragments arranged by size:
3070 1484 208 165 55 54
BsrDI GCAATG_nn'
Cuts at: 299 653 2948 299
Size: 354 2295 2387
Fragments arranged by size:
2387 2295 354
BsrFI r'CCGG_y
Cuts at: 1597 3034 3215 3424 1597
Size: 1437 181 209 3209
Fragments arranged by size:
3209 1437 209 181
BsrGI T'GTAC_A
Cuts at: 360 360
Size: 5036
BssSI C'ACGA_G
Cuts at: 3307 3665 3307
Size: 358 4678
BstAPI GCAn_nnn'nTGC
Cuts at: 911 1158 1732 2413 911
Size: 247 574 681 3534
Fragments arranged by size:
3534 681 574 247
BstBI TT'CG_AA
Cuts at: 3397 3397
Size: 5036
BstXI CCAn_nnnn'nTGG
Cuts at: 237 1254 237
Size: 1017 4019
BstYI r'GATC_y
Cuts at: 1 2061 2528 2551 2886 3132 4144 4230 4242
Size: 2060 467 23 335 246 1012 86 12
Cuts at: 4242 4588 1
Size: 346 449
Fragments arranged by size:
2060 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 461 461
Size: 5036
BtgI C'CryG_G
Cuts at: 107 641 1583 107
Size: 534 942 3560
Fragments arranged by size:
3560 942 534
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 183 1605 2986 3167 3330 183
Size: 1422 1381 181 163 1889
Fragments arranged by size:
1889 1422 1381 181 163
BtsI GCAGTG_nn'
Cuts at: 545 776 2279 545
Size: 231 1503 3302
Fragments arranged by size:
3302 1503 231
DraI TTT'AAA
Cuts at: 2035 2035
Size: 5036
DraIII CAC_nnn'GTG
Cuts at: 837 837
Size: 5036
DrdI GACnn_nn'nnGTC
Cuts at: 936 2742 3600 936
Size: 1806 858 2372
Fragments arranged by size:
2372 1806 858
EaeI y'GGCC_r
Cuts at: 275 2621 2795 3186 3213 4558 275
Size: 2346 174 391 27 1345 753
Fragments arranged by size:
2346 1345 753 391 174 27
EagI C'GGCC_G
Cuts at: 2621 2621
Size: 5036
EarI CTCTTCn'nnn_
Cuts at: 477 1016 1756 3059 3269 477
Size: 539 740 1303 210 2244
Fragments arranged by size:
2244 1303 740 539 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3244 3554 3700 4846 3244
Size: 310 146 1146 3434
Fragments arranged by size:
3434 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1012 1842 2048 1012
Size: 830 206 4000
Fragments arranged by size:
4000 830 206
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1075 1147 1342 1493 1715 1876 2861 3293 4040
Size: 72 195 151 222 161 985 432 747
Cuts at: 4040 4308 1075
Size: 268 1803
Fragments arranged by size:
1803 985 747 432 268 222 195 161
151 72
EcoNI CCTnn'n_nnAGG
Cuts at: 915 1645 2003 915
Size: 730 358 3948
Fragments arranged by size:
3948 730 358
EcoO109I rG'GnC_Cy
Cuts at: 929 1281 2008 2175 929
Size: 352 727 167 3790
Fragments arranged by size:
3790 727 352 167
EcoRI G'AATT_C
Cuts at: 2040 2040
Size: 5036
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1752 3055 1752
Size: 1303 3733
FspI TGC'GCA
Cuts at: 904 2817 904
Size: 1913 3123
HaeII r_GCGC'y
Cuts at: 1075 2470 2718 3740 4719 1075
Size: 1395 248 1022 979 1392
Fragments arranged by size:
1395 1392 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 693 725 1172 1204 2878 2908 2910 2940 693
Size: 32 447 32 1674 30 2 30 2789
Fragments arranged by size:
2789 1674 447 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2075 2075
Size: 5036
HindIII A'AGCT_T
Cuts at: 449 449
Size: 5036
KasI G'GCGC_C
Cuts at: 2466 2714 2466
Size: 248 4788
KpnI G_GTAC'C
Cuts at: 1052 2056 1052
Size: 1004 4032
MfeI C'AATT_G
Cuts at: 2015 2015
Size: 5036
MluI A'CGCG_T
Cuts at: 4469 4469
Size: 5036
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3707 3891 3707
Size: 184 4852
MscI TGG'CCA
Cuts at: 2797 2797
Size: 5036
MslI CAynn'nnrTG
Cuts at: 797 1252 1582 3025 3152 797
Size: 455 330 1443 127 2681
Fragments arranged by size:
2681 1443 455 330 127
MspA1I CmG'CkG
Cuts at: 1155 2147 2461 2821 3834 4079 1155
Size: 992 314 360 1013 245 2112
Fragments arranged by size:
2112 1013 992 360 314 245
NaeI GCC'GGC
Cuts at: 3217 3217
Size: 5036
NarI GG'CG_CC
Cuts at: 2467 2715 2467
Size: 248 4788
NcoI C'CATG_G
Cuts at: 107 1583 107
Size: 1476 3560
NdeI CA'TA_TG
Cuts at: 1224 1224
Size: 5036
NgoMIV G'CCGG_C
Cuts at: 3215 3215
Size: 5036
NspI r_CATG'y
Cuts at: 697 2089 2589 3496 697
Size: 1392 500 907 2237
Fragments arranged by size:
2237 1392 907 500
PasI CC'CwG_GG
Cuts at: 1314 1517 1314
Size: 203 4833
PciI A'CATG_T
Cuts at: 693 3492 693
Size: 2799 2237
PflMI CCAn_nnn'nTGG
Cuts at: 837 837
Size: 5036
PmeI GTTT'AAAC
Cuts at: 2035 2035
Size: 5036
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4175 4207 4175
Size: 32 5004
PpuMI rG'GwC_Cy
Cuts at: 929 1281 929
Size: 352 4684
PstI C_TGCA'G
Cuts at: 2083 2768 2083
Size: 685 4351
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5036
PvuII CAG'CTG
Cuts at: 2461 2821 2461
Size: 360 4676
RsrII CG'GwC_CG
Cuts at: 3231 3231
Size: 5036
SacI G_AGCT'C
Cuts at: 1014 1844 2050 1014
Size: 830 206 4000
Fragments arranged by size:
4000 830 206
SalI G'TCGA_C
Cuts at: 2073 2073
Size: 5036
SapI GCTCTTCn'nnn_
Cuts at: 3059 3269 3059
Size: 210 4826
SbfI CC_TGCA'GG
Cuts at: 2083 2083
Size: 5036
ScaI AGT'ACT
Cuts at: 4553 4553
Size: 5036
SexAI A'CCwGG_T
Cuts at: 284 284
Size: 5036
SfcI C'TryA_G
Cuts at: 33 1061 2079 2764 3757 3948 33
Size: 1028 1018 685 993 191 1121
Fragments arranged by size:
1121 1028 1018 993 685 191
SfoI GGC'GCC
Cuts at: 2468 2716 2468
Size: 248 4788
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5036
SmaI CCC'GGG
Cuts at: 1422 2058 1422
Size: 636 4400
SmlI C'TyrA_G
Cuts at: 2191 3106 3598 3860 2191
Size: 915 492 262 3367
Fragments arranged by size:
3367 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5036
SphI G_CATG'C
Cuts at: 2089 2589 2089
Size: 500 4536
SspI AAT'ATT
Cuts at: 1823 1823
Size: 5036
StuI AGG'CCT
Cuts at: 511 1998 511
Size: 1487 3549
StyI C'CwwG_G
Cuts at: 107 119 1145 1583 1947 2170 2524 107
Size: 12 1026 438 364 223 354 2619
Fragments arranged by size:
2619 1026 438 364 354 223 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 703 787 961 1530 2619 2752 3211 3420 703
Size: 84 174 569 1089 133 459 209 2319
Fragments arranged by size:
2319 1089 569 459 209 174 133 84
TatI w'GTAC_w
Cuts at: 360 4551 360
Size: 4191 845
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 602 634 1903 1935 4553 4585 602
Size: 32 1269 32 2618 32 1053
Fragments arranged by size:
2618 1269 1053 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 933 2833 933
Size: 1900 3136
XbaI T'CTAG_A
Cuts at: 2067 2067
Size: 5036
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2531 2531
Size: 5036
XmaI C'CCGG_G
Cuts at: 1420 2056 1420
Size: 636 4400
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PflMI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI ApaI AscI AvrII BaeI
BbsI BcgI BfrBI BmtI BsiWI BssHII BstEII BstZ17I
ClaI CspCI EcoRV FseI FspAI HpaI NheI NotI
NruI NsiI PacI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SwaI XhoI XmnI ZraI