Restriction map for
pF1KB8679
(Circular) (Six-base) MAPSORT of: pF1KB8679.seq Check: 5232 from: 1 to: 4655
pF1KB8679 4655 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1341 1341
Size: 4655
AccI GT'mk_AC
Cuts at: 1693 1693
Size: 4655
Acc65I G'GTAC_C
Cuts at: 1671 1671
Size: 4655
AclI AA'CG_TT
Cuts at: 4495 4495
Size: 4655
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 364 1415 2480 2912 3659 3927 227
Size: 137 1051 1065 432 747 268 955
Fragments arranged by size:
1065 1051 955 747 432 268 137
AflIII A'CryG_T
Cuts at: 251 2007 3111 4088 251
Size: 1756 1104 977 818
Fragments arranged by size:
1756 1104 977 818
AgeI A'CCGG_T
Cuts at: 3043 3043
Size: 4655
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1436 1436
Size: 4655
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 840 872 840
Size: 32 4623
AlwNI CAG_nnn'CTG
Cuts at: 921 1960 3527 921
Size: 1039 1567 2049
Fragments arranged by size:
2049 1567 1039
ApaI G_GGCC'C
Cuts at: 624 624
Size: 4655
ApaLI G'TGCA_C
Cuts at: 799 3425 799
Size: 2626 2029
ApoI r'AATT_y
Cuts at: 211 279 1659 4008 4264 211
Size: 68 1380 2349 256 602
Fragments arranged by size:
2349 1380 602 256 68
AseI AT'TA_AT
Cuts at: 20 383 1153 20
Size: 363 770 3522
Fragments arranged by size:
3522 770 363
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4655
AvaI C'yCGr_G
Cuts at: 1675 1675
Size: 4655
BamHI G'GATC_C
Cuts at: 480 1680 480
Size: 1200 3455
BanI G'GyrC_C
Cuts at: 640 775 1671 2085 2333 2368 640
Size: 135 896 414 248 35 2927
Fragments arranged by size:
2927 896 414 248 135 35
BanII G_rGCy'C
Cuts at: 624 1669 2699 624
Size: 1045 1030 2580
Fragments arranged by size:
2580 1045 1030
BbeI G_GCGC'C
Cuts at: 2089 2337 2089
Size: 248 4407
BbsI GAAGACnn'nnnn_
Cuts at: 463 463
Size: 4655
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 826 860 826
Size: 34 4621
BciVI GTATCCnnnnn_n'
Cuts at: 434 2553 3314 4655 434
Size: 2119 761 1341 434
Fragments arranged by size:
2119 1341 761 434
BclI T'GATC_A
Cuts at: 1214 1283 1502 2175 1214
Size: 69 219 673 3694
Fragments arranged by size:
3694 673 219 69
BfrBI ATG'CAT
Cuts at: 897 897
Size: 4655
BglII A'GATC_T
Cuts at: 2170 4207 2170
Size: 2037 2618
BlpI GC'TnA_GC
Cuts at: 1767 1767
Size: 4655
Bme1580I G_kGCm'C
Cuts at: 467 624 803 2280 2373 3429 467
Size: 157 179 1477 93 1056 1693
Fragments arranged by size:
1693 1477 1056 179 157 93
BmgBI CAC'GTC
Cuts at: 254 254
Size: 4655
BmrI ACTGGGnnnn_n'
Cuts at: 1867 1948 2283 4427 1867
Size: 81 335 2144 2095
Fragments arranged by size:
2144 2095 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3022 3022
Size: 4655
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 881 1469 1576 1595 881
Size: 588 107 19 3941
Fragments arranged by size:
3941 588 107 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1831 2710 3202 3500 1831
Size: 879 492 298 2986
Fragments arranged by size:
2986 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4359 4359
Size: 4655
BsaAI yAC'GTr
Cuts at: 2008 2008
Size: 4655
BsaBI GATnn'nnATC
Cuts at: 6 2169 4206 6
Size: 2163 2037 455
Fragments arranged by size:
2163 2037 455
BsaHI Gr'CG_yC
Cuts at: 2086 2334 3036 4532 2086
Size: 248 702 1496 2209
Fragments arranged by size:
2209 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1844 2365 3043 3317 3464 3875 1844
Size: 521 678 274 147 411 2624
Fragments arranged by size:
2624 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 151 181 840 870 151
Size: 30 659 30 3936
Fragments arranged by size:
3936 659 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1472 1484 1472
Size: 12 4643
BseYI C'CCAG_C
Cuts at: 608 2080 2838 3415 3911 608
Size: 1472 758 577 496 1352
Fragments arranged by size:
1472 1352 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 572 1040 568
Size: 4 468 4183
Fragments arranged by size:
4183 468 4
BsiEI CG_ry'CG
Cuts at: 104 2197 2243 3451 104
Size: 2093 46 1208 1308
Fragments arranged by size:
2093 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 803 1669 2447 2637 3429 803
Size: 866 778 190 792 2029
Fragments arranged by size:
2029 866 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 260 260
Size: 4655
Bsp1286I G_dGCh'C
Cuts at: 467 624 803 1669 2280 2373 2447 2637 2699
Size: 157 179 866 611 93 74 190 62
Cuts at: 2699 3429 467
Size: 730 1693
Fragments arranged by size:
1693 866 730 611 190 179 157 93
74 62
BspEI T'CCGG_A
Cuts at: 3875 3875
Size: 4655
BspHI T'CATG_A
Cuts at: 866 3831 866
Size: 2965 1690
BspMI ACCTGCnnnn'nnnn_
Cuts at: 959 1186 1341 1404 1705 1919 2221 2602 959
Size: 227 155 63 301 214 302 381 3012
Fragments arranged by size:
3012 381 302 301 227 214 155 63
BsrBI CCG'CTC
Cuts at: 50 162 1562 2947 3001 4485 4650 50
Size: 112 1400 1385 54 1484 165 55
Fragments arranged by size:
1484 1400 1385 165 112 55 54
BsrDI GCAATG_nn'
Cuts at: 169 2567 169
Size: 2398 2257
BsrFI r'CCGG_y
Cuts at: 2653 2834 3043 2653
Size: 181 209 4265
Fragments arranged by size:
4265 209 181
BssSI C'ACGA_G
Cuts at: 2926 3284 2926
Size: 358 4297
BstAPI GCAn_nnn'nTGC
Cuts at: 903 1027 1438 2032 903
Size: 124 411 594 3526
Fragments arranged by size:
3526 594 411 124
BstBI TT'CG_AA
Cuts at: 3016 3016
Size: 4655
BstXI CCAn_nnnn'nTGG
Cuts at: 531 531
Size: 4655
BstYI r'GATC_y
Cuts at: 1 480 1680 2147 2170 2505 2751 3763 3849
Size: 479 1200 467 23 335 246 1012 86
Cuts at: 3849 3861 4207 1
Size: 12 346 449
Fragments arranged by size:
1200 1012 479 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 1185 107
Size: 1078 3577
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2605 2786 2949 2605
Size: 181 163 4311
Fragments arranged by size:
4311 181 163
BtsI GCAGTG_nn'
Cuts at: 1898 1898
Size: 4655
DraI TTT'AAA
Cuts at: 1654 1654
Size: 4655
DraIII CAC_nnn'GTG
Cuts at: 888 888
Size: 4655
DrdI GACnn_nn'nnGTC
Cuts at: 2361 3219 2361
Size: 858 3797
EaeI y'GGCC_r
Cuts at: 2240 2414 2805 2832 4177 2240
Size: 174 391 27 1345 2718
Fragments arranged by size:
2718 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2240 2240
Size: 4655
EarI CTCTTCn'nnn_
Cuts at: 340 414 1516 2678 2888 340
Size: 74 1102 1162 210 2107
Fragments arranged by size:
2107 1162 1102 210 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 2863 3173 3319 4465 127
Size: 2736 310 146 1146 317
Fragments arranged by size:
2736 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 1667 1667
Size: 4655
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 364 881 1415 1469 1576 1595 2480 2912
Size: 137 517 534 54 107 19 885 432
Cuts at: 2912 3659 3927 227
Size: 747 268 955
Fragments arranged by size:
955 885 747 534 517 432 268 137
107 54 19
EcoO109I rG'GnC_Cy
Cuts at: 605 620 621 1293 1794 605
Size: 15 1 672 501 3466
Fragments arranged by size:
3466 672 501 15 1
EcoRI G'AATT_C
Cuts at: 1659 1659
Size: 4655
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 232 2674 232
Size: 2442 2213
FspI TGC'GCA
Cuts at: 2436 2436
Size: 4655
HaeII r_GCGC'y
Cuts at: 2089 2337 3359 4338 2089
Size: 248 1022 979 2406
Fragments arranged by size:
2406 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 151 183 382 414 734 766 1118 1150 2497
Size: 32 199 32 320 32 352 32 1347
Cuts at: 2497 2527 2529 2559 151
Size: 30 2 30 2247
Fragments arranged by size:
2247 1347 352 320 199 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 986 1359 1694 986
Size: 373 335 3947
Fragments arranged by size:
3947 373 335
KasI G'GCGC_C
Cuts at: 2085 2333 2085
Size: 248 4407
KpnI G_GTAC'C
Cuts at: 1675 1675
Size: 4655
MluI A'CGCG_T
Cuts at: 4088 4088
Size: 4655
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 227 3326 3510 227
Size: 3099 184 1372
Fragments arranged by size:
3099 1372 184
MscI TGG'CCA
Cuts at: 2416 2416
Size: 4655
MslI CAynn'nnrTG
Cuts at: 938 2644 2771 938
Size: 1706 127 2822
Fragments arranged by size:
2822 1706 127
MspA1I CmG'CkG
Cuts at: 1165 1766 2080 2440 3453 3698 1165
Size: 601 314 360 1013 245 2122
Fragments arranged by size:
2122 1013 601 360 314 245
NaeI GCC'GGC
Cuts at: 2836 2836
Size: 4655
NarI GG'CG_CC
Cuts at: 2086 2334 2086
Size: 248 4407
NcoI C'CATG_G
Cuts at: 107 1185 107
Size: 1078 3577
NgoMIV G'CCGG_C
Cuts at: 2834 2834
Size: 4655
NsiI A_TGCA'T
Cuts at: 899 899
Size: 4655
NspI r_CATG'y
Cuts at: 329 1708 2208 3115 329
Size: 1379 500 907 1869
Fragments arranged by size:
1869 1379 907 500
PciI A'CATG_T
Cuts at: 3111 3111
Size: 4655
PflMI CCAn_nnn'nTGG
Cuts at: 420 420
Size: 4655
PmeI GTTT'AAAC
Cuts at: 1654 1654
Size: 4655
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 840 872 3794 3826 840
Size: 32 2922 32 1669
Fragments arranged by size:
2922 1669 32 32
PpuMI rG'GwC_Cy
Cuts at: 605 605
Size: 4655
PspOMI G'GGCC_C
Cuts at: 620 620
Size: 4655
PstI C_TGCA'G
Cuts at: 675 1702 2387 675
Size: 1027 685 2943
Fragments arranged by size:
2943 1027 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4655
PvuII CAG'CTG
Cuts at: 2080 2440 2080
Size: 360 4295
RsrII CG'GwC_CG
Cuts at: 2850 2850
Size: 4655
SacI G_AGCT'C
Cuts at: 1669 1669
Size: 4655
SalI G'TCGA_C
Cuts at: 1692 1692
Size: 4655
SanDI GG'GwC_CC
Cuts at: 605 605
Size: 4655
SapI GCTCTTCn'nnn_
Cuts at: 2678 2888 2678
Size: 210 4445
SbfI CC_TGCA'GG
Cuts at: 1702 1702
Size: 4655
ScaI AGT'ACT
Cuts at: 4172 4172
Size: 4655
SfcI C'TryA_G
Cuts at: 33 671 1362 1698 2383 3376 3567 33
Size: 638 691 336 685 993 191 1121
Fragments arranged by size:
1121 993 691 685 638 336 191
SfoI GGC'GCC
Cuts at: 2087 2335 2087
Size: 248 4407
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4655
SmaI CCC'GGG
Cuts at: 1677 1677
Size: 4655
SmlI C'TyrA_G
Cuts at: 1810 2725 3217 3479 1810
Size: 915 492 262 2986
Fragments arranged by size:
2986 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4655
SphI G_CATG'C
Cuts at: 1708 2208 1708
Size: 500 4155
StyI C'CwwG_G
Cuts at: 107 485 804 1185 1789 2143 107
Size: 378 319 381 604 354 2619
Fragments arranged by size:
2619 604 381 378 354 319
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 747 2238 2371 2830 3039 747
Size: 1491 133 459 209 2363
Fragments arranged by size:
2363 1491 459 209 133
TatI w'GTAC_w
Cuts at: 4170 4170
Size: 4655
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 780 812 4172 4204 780
Size: 32 3360 32 1231
Fragments arranged by size:
3360 1231 32 32
Tth111I GACn'n_nGTC
Cuts at: 2452 2452
Size: 4655
XbaI T'CTAG_A
Cuts at: 1686 1686
Size: 4655
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1383 1551 2150 1383
Size: 168 599 3888
Fragments arranged by size:
3888 599 168
XmaI C'CCGG_G
Cuts at: 1675 1675
Size: 4655
XmnI GAAnn'nnTTC
Cuts at: 212 212
Size: 4655
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PflMI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AscI AvrII BaeI
BbvCI BglI BmtI Bpu10I BsiWI BsmI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PasI PfoI PmlI PshAI PsiI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI