(Circular) (Six-base) MAPSORT of: pF1KB8675.seq Check: 6251 from: 1 to: 4979 pF1KB8675 4979 bp With 182 enzymes: SgfI * June 20, 2008 10:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 390 899 390 Size: 509 4470 AatII G_ACGT'C Cuts at: 584 802 584 Size: 218 4761 AccI GT'mk_AC Cuts at: 1436 2017 1436 Size: 581 4398 Acc65I G'GTAC_C Cuts at: 1995 1995 Size: 4979 AclI AA'CG_TT Cuts at: 4819 4819 Size: 4979 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1258 2804 3236 3589 4251 1258 Size: 1546 432 353 662 1986 Fragments arranged by size: 1986 1546 662 432 353 AfeI AGC'GCT Cuts at: 277 277 Size: 4979 AflIII A'CryG_T Cuts at: 2331 4131 4412 2331 Size: 1800 281 2898 Fragments arranged by size: 2898 1800 281 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 304 1798 304 Size: 1494 3485 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 592 624 592 Size: 32 4947 AlwNI CAG_nnn'CTG Cuts at: 189 390 432 465 2284 3722 189 Size: 201 42 33 1819 1438 1446 Fragments arranged by size: 1819 1446 1438 201 42 33 ApaI G_GGCC'C Cuts at: 1187 1479 1187 Size: 292 4687 ApaLI G'TGCA_C Cuts at: 3817 3817 Size: 4979 ApoI r'AATT_y Cuts at: 1983 4332 4588 1983 Size: 2349 256 2374 Fragments arranged by size: 2374 2349 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4979 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4979 AvaI C'yCGr_G Cuts at: 496 1180 1675 1999 496 Size: 684 495 324 3476 Fragments arranged by size: 3476 684 495 324 BamHI G'GATC_C Cuts at: 2004 2004 Size: 4979 BanI G'GyrC_C Cuts at: 166 1487 1648 1807 1995 2409 2657 2692 166 Size: 1321 161 159 188 414 248 35 2453 Fragments arranged by size: 2453 1321 414 248 188 161 159 35 BanII G_rGCy'C Cuts at: 634 1187 1479 1823 1993 3023 634 Size: 553 292 344 170 1030 2590 Fragments arranged by size: 2590 1030 553 344 292 170 BbeI G_GCGC'C Cuts at: 1491 2413 2661 1491 Size: 922 248 3809 Fragments arranged by size: 3809 922 248 BbsI GAAGACnn'nnnn_ Cuts at: 557 791 802 557 Size: 234 11 4734 Fragments arranged by size: 4734 234 11 BbvCI CC'TCA_GC Cuts at: 1127 1127 Size: 4979 BciVI GTATCCnnnnn_n' Cuts at: 2877 3933 4979 2877 Size: 1056 1046 2877 Fragments arranged by size: 2877 1056 1046 BclI T'GATC_A Cuts at: 2499 2499 Size: 4979 BglI GCCn_nnn'nGGC Cuts at: 223 223 Size: 4979 BglII A'GATC_T Cuts at: 1163 2494 4531 1163 Size: 1331 2037 1611 Fragments arranged by size: 2037 1611 1331 BlpI GC'TnA_GC Cuts at: 142 1010 1087 2091 142 Size: 868 77 1004 3030 Fragments arranged by size: 3030 1004 868 77 Bme1580I G_kGCm'C Cuts at: 169 219 1032 1158 1187 1479 1810 2604 2697 Size: 50 813 126 29 292 331 794 93 Cuts at: 2697 3821 169 Size: 1124 1327 Fragments arranged by size: 1327 1124 813 794 331 292 126 93 50 29 BmgBI CAC'GTC Cuts at: 1683 1683 Size: 4979 BmrI ACTGGGnnnn_n' Cuts at: 937 2191 2272 2607 4751 937 Size: 1254 81 335 2144 1165 Fragments arranged by size: 2144 1254 1165 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 282 1329 3346 282 Size: 1047 2017 1915 Fragments arranged by size: 2017 1915 1047 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 211 811 1054 1180 1916 1917 211 Size: 600 243 126 736 1 3273 Fragments arranged by size: 3273 736 600 243 126 1 Bpu10I CC'TnA_GC Cuts at: 1127 1127 Size: 4979 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1746 2155 3034 3748 4046 1746 Size: 409 879 714 298 2679 Fragments arranged by size: 2679 879 714 409 298 BsaI GGTCTCn'nnnn_ Cuts at: 1258 1610 1912 4683 1258 Size: 352 302 2771 1554 Fragments arranged by size: 2771 1554 352 302 BsaAI yAC'GTr Cuts at: 2332 2332 Size: 4979 BsaBI GATnn'nnATC Cuts at: 6 2493 4530 6 Size: 2487 2037 455 Fragments arranged by size: 2487 2037 455 BsaHI Gr'CG_yC Cuts at: 408 554 581 799 1488 2410 2658 3360 4856 Size: 146 27 218 689 922 248 702 1496 Cuts at: 4856 408 Size: 531 Fragments arranged by size: 1496 922 702 689 531 248 218 146 27 BsaWI w'CCGG_w Cuts at: 2168 2689 3367 3778 3925 4199 2168 Size: 521 678 411 147 274 2948 Fragments arranged by size: 2948 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 412 442 644 674 784 814 1255 1285 412 Size: 30 202 30 110 30 441 30 4106 Fragments arranged by size: 4106 441 202 110 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 148 218 454 514 517 583 665 1144 1369 Size: 70 236 60 3 66 82 479 225 Cuts at: 1369 148 Size: 3758 Fragments arranged by size: 3758 479 236 225 82 70 66 60 3 BseYI C'CCAG_C Cuts at: 162 321 491 633 942 1642 1889 2404 3162 Size: 159 170 142 309 700 247 515 758 Cuts at: 3162 3827 4235 162 Size: 665 408 906 Fragments arranged by size: 906 758 700 665 515 408 309 247 170 159 142 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 436 694 436 Size: 258 4721 BsiEI CG_ry'CG Cuts at: 104 2521 2567 3797 104 Size: 2417 46 1230 1286 Fragments arranged by size: 2417 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 1993 2771 2961 3821 1993 Size: 778 190 860 3151 Fragments arranged by size: 3151 860 778 190 Bsp1286I G_dGCh'C Cuts at: 169 219 634 1032 1158 1187 1479 1810 1823 Size: 50 415 398 126 29 292 331 13 Cuts at: 1823 1993 2604 2697 2771 2961 3023 3821 169 Size: 170 611 93 74 190 62 798 1327 Fragments arranged by size: 1327 798 611 415 398 331 292 190 170 126 93 74 62 50 29 13 BspHI T'CATG_A Cuts at: 524 1296 3411 524 Size: 772 2115 2092 Fragments arranged by size: 2115 2092 772 BspMI ACCTGCnnnn'nnnn_ Cuts at: 390 899 2029 2243 2545 2926 390 Size: 509 1130 214 302 381 2443 Fragments arranged by size: 2443 1130 509 381 302 214 BsrBI CCG'CTC Cuts at: 50 1359 3271 3325 4809 4974 50 Size: 1309 1912 54 1484 165 55 Fragments arranged by size: 1912 1484 1309 165 55 54 BsrDI GCAATG_nn' Cuts at: 2891 2891 Size: 4979 BsrFI r'CCGG_y Cuts at: 247 1484 2977 3158 247 Size: 1237 1493 181 2068 Fragments arranged by size: 2068 1493 1237 181 BsrGI T'GTAC_A Cuts at: 1271 1271 Size: 4979 BssSI C'ACGA_G Cuts at: 3250 3958 3250 Size: 708 4271 BstAPI GCAn_nnn'nTGC Cuts at: 390 465 516 2356 390 Size: 75 51 1840 3013 Fragments arranged by size: 3013 1840 75 51 BstBI TT'CG_AA Cuts at: 3340 3340 Size: 4979 BstXI CCAn_nnnn'nTGG Cuts at: 1941 1941 Size: 4979 BstYI r'GATC_y Cuts at: 1 533 686 1037 1163 1280 2004 2471 2494 Size: 532 153 351 126 117 724 467 23 Cuts at: 2494 2829 3075 3381 3393 3479 4531 1 Size: 335 246 306 12 86 1052 449 Fragments arranged by size: 1052 724 532 467 449 351 335 306 246 153 126 117 86 23 12 Bsu36I CC'TnA_GG Cuts at: 242 1765 242 Size: 1523 3456 BtgI C'CryG_G Cuts at: 107 127 485 947 1940 107 Size: 20 358 462 993 3146 Fragments arranged by size: 3146 993 462 358 20 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2929 3110 3273 2929 Size: 181 163 4635 Fragments arranged by size: 4635 181 163 BtsI GCAGTG_nn' Cuts at: 480 1608 1722 1782 2222 480 Size: 1128 114 60 440 3237 Fragments arranged by size: 3237 1128 440 114 60 DraI TTT'AAA Cuts at: 1978 1978 Size: 4979 DrdI GACnn_nn'nnGTC Cuts at: 2685 4029 2685 Size: 1344 3635 EaeI y'GGCC_r Cuts at: 110 908 1076 1202 2564 2738 3129 3156 4501 Size: 798 168 126 1362 174 391 27 1345 Cuts at: 4501 110 Size: 588 Fragments arranged by size: 1362 1345 798 588 391 174 168 126 27 EagI C'GGCC_G Cuts at: 2564 2564 Size: 4979 EarI CTCTTCn'nnn_ Cuts at: 1874 3002 3212 1874 Size: 1128 210 3641 Fragments arranged by size: 3641 1128 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3187 3929 4075 4789 3187 Size: 742 146 714 3377 Fragments arranged by size: 3377 742 714 146 EcoICRI GAG'CTC Cuts at: 1991 1991 Size: 4979 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 211 811 1054 1180 1258 1916 1917 2804 3236 Size: 600 243 126 78 658 1 887 432 Cuts at: 3236 3589 4251 211 Size: 353 662 939 Fragments arranged by size: 939 887 662 658 600 432 353 243 126 78 1 EcoNI CCTnn'n_nnAGG Cuts at: 644 1240 644 Size: 596 4383 EcoO109I rG'GnC_Cy Cuts at: 1050 1475 1476 1687 2118 1050 Size: 425 1 211 431 3911 Fragments arranged by size: 3911 431 425 211 1 EcoRI G'AATT_C Cuts at: 1983 1983 Size: 4979 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 531 2998 531 Size: 2467 2512 FspI TGC'GCA Cuts at: 1465 2760 1465 Size: 1295 3684 HaeII r_GCGC'y Cuts at: 279 1383 1394 1491 2413 2661 3891 4662 279 Size: 1104 11 97 922 248 1230 771 596 Fragments arranged by size: 1230 1104 922 771 596 248 97 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 784 816 2821 2851 2853 2883 784 Size: 32 2005 30 2 30 2880 Fragments arranged by size: 2880 2005 32 30 30 2 HincII GTy'rAC Cuts at: 1452 2018 1452 Size: 566 4413 KasI G'GCGC_C Cuts at: 1487 2409 2657 1487 Size: 922 248 3809 Fragments arranged by size: 3809 922 248 KpnI G_GTAC'C Cuts at: 1999 1999 Size: 4979 MluI A'CGCG_T Cuts at: 4412 4412 Size: 4979 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 524 3738 3922 524 Size: 3214 184 1581 Fragments arranged by size: 3214 1581 184 MscI TGG'CCA Cuts at: 112 910 1078 1204 2740 112 Size: 798 168 126 1536 2351 Fragments arranged by size: 2351 1536 798 168 126 MslI CAynn'nnrTG Cuts at: 112 118 1342 2968 3095 112 Size: 6 1224 1626 127 1996 Fragments arranged by size: 1996 1626 1224 127 6 MspA1I CmG'CkG Cuts at: 129 1415 1696 1736 2090 2404 2764 3548 3793 Size: 1286 281 40 354 314 360 784 245 Cuts at: 3793 129 Size: 1315 Fragments arranged by size: 1315 1286 784 360 354 314 281 245 40 NaeI GCC'GGC Cuts at: 249 3160 249 Size: 2911 2068 NarI GG'CG_CC Cuts at: 1488 2410 2658 1488 Size: 922 248 3809 Fragments arranged by size: 3809 922 248 NcoI C'CATG_G Cuts at: 107 1940 107 Size: 1833 3146 NgoMIV G'CCGG_C Cuts at: 247 3158 247 Size: 2911 2068 NspI r_CATG'y Cuts at: 2032 2532 4135 2032 Size: 500 1603 2876 Fragments arranged by size: 2876 1603 500 PciI A'CATG_T Cuts at: 4131 4131 Size: 4979 PflMI CCAn_nnn'nTGG Cuts at: 189 189 Size: 4979 PfoI T'CCnGG_A Cuts at: 914 1040 1754 914 Size: 126 714 4139 Fragments arranged by size: 4139 714 126 PmeI GTTT'AAAC Cuts at: 1978 1978 Size: 4979 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3425 3457 3425 Size: 32 4947 PpuMI rG'GwC_Cy Cuts at: 1050 1687 1050 Size: 637 4342 PspOMI G'GGCC_C Cuts at: 1183 1475 1183 Size: 292 4687 PspXI vC'TCGA_Gb Cuts at: 496 1675 496 Size: 1179 3800 PstI C_TGCA'G Cuts at: 470 1802 2026 2711 470 Size: 1332 224 685 2738 Fragments arranged by size: 2738 1332 685 224 PvuI CG_AT'CG Cuts at: 104 104 Size: 4979 PvuII CAG'CTG Cuts at: 1415 1696 2404 2764 1415 Size: 281 708 360 3630 Fragments arranged by size: 3630 708 360 281 RsrII CG'GwC_CG Cuts at: 3174 3174 Size: 4979 SacI G_AGCT'C Cuts at: 1993 1993 Size: 4979 SacII CC_GC'GG Cuts at: 130 130 Size: 4979 SalI G'TCGA_C Cuts at: 2016 2016 Size: 4979 SapI GCTCTTCn'nnn_ Cuts at: 1874 3002 3212 1874 Size: 1128 210 3641 Fragments arranged by size: 3641 1128 210 SbfI CC_TGCA'GG Cuts at: 2026 2026 Size: 4979 ScaI AGT'ACT Cuts at: 4496 4496 Size: 4979 SexAI A'CCwGG_T Cuts at: 716 716 Size: 4979 SfcI C'TryA_G Cuts at: 33 368 466 1691 1798 2022 2707 3675 3866 Size: 335 98 1225 107 224 685 968 191 Cuts at: 3866 33 Size: 1146 Fragments arranged by size: 1225 1146 968 685 335 224 191 107 98 SfoI GGC'GCC Cuts at: 1489 2411 2659 1489 Size: 922 248 3809 Fragments arranged by size: 3809 922 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4979 SmaI CCC'GGG Cuts at: 2001 2001 Size: 4979 SmlI C'TyrA_G Cuts at: 496 1675 1725 2134 3049 3763 4025 496 Size: 1179 50 409 915 714 262 1450 Fragments arranged by size: 1450 1179 915 714 409 262 50 SpeI A'CTAG_T Cuts at: 67 67 Size: 4979 SphI G_CATG'C Cuts at: 2032 2532 2032 Size: 500 4479 SspI AAT'ATT Cuts at: 1141 1141 Size: 4979 StuI AGG'CCT Cuts at: 1426 1426 Size: 4979 StyI C'CwwG_G Cuts at: 107 170 1574 1940 2113 2467 107 Size: 63 1404 366 173 354 2619 Fragments arranged by size: 2619 1404 366 354 173 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 956 1649 2562 2695 3154 3363 956 Size: 693 913 133 459 209 2572 Fragments arranged by size: 2572 913 693 459 209 133 TatI w'GTAC_w Cuts at: 872 998 1271 4494 872 Size: 126 273 3223 1357 Fragments arranged by size: 3223 1357 273 126 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 412 444 644 676 1022 1054 4496 4528 412 Size: 32 200 32 346 32 3442 32 863 Fragments arranged by size: 3442 863 346 200 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 2776 2776 Size: 4979 XbaI T'CTAG_A Cuts at: 2010 2010 Size: 4979 XcmI CCAnnnn_n'nnnnTGG Cuts at: 114 594 1218 2474 114 Size: 480 624 1256 2619 Fragments arranged by size: 2619 1256 624 480 XhoI C'TCGA_G Cuts at: 496 1675 496 Size: 1179 3800 XmaI C'CCGG_G Cuts at: 1999 1999 Size: 4979 ZraI GAC'GTC Cuts at: 582 800 582 Size: 218 4761 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflIII AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI Enzymes that do not cut: AflII AgeI AhdI AleI AscI AvrII BaeI BcgI BfrBI BmtI BsiWI BsmI BsmBI BspEI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PmlI PshAI PsiI PsrI SanDI SfiI SgrAI SnaBI SrfI SwaI XmnI