Restriction map for pF1KB8675
 (Circular) (Six-base) MAPSORT of: pF1KB8675.seq  Check: 6251  from: 1  to: 4979



pF1KB8675  4979 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    390     899     390 
   Size:        509    4470
 
AatII G_ACGT'C

Cuts at:    584     802     584 
   Size:        218    4761
 
AccI GT'mk_AC

Cuts at:   1436    2017    1436 
   Size:        581    4398
 
Acc65I G'GTAC_C

Cuts at:   1995    1995 
   Size:       4979
 
AclI AA'CG_TT

Cuts at:   4819    4819 
   Size:       4979
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1258    2804    3236    3589    4251    1258 
   Size:       1546     432     353     662    1986

  Fragments arranged by size: 

                1986    1546     662     432     353
 
AfeI AGC'GCT

Cuts at:    277     277 
   Size:       4979
 
AflIII A'CryG_T

Cuts at:   2331    4131    4412    2331 
   Size:       1800     281    2898

  Fragments arranged by size: 

                2898    1800     281
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    304    1798     304 
   Size:       1494    3485
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    592     624     592 
   Size:         32    4947
 
AlwNI CAG_nnn'CTG

Cuts at:    189     390     432     465    2284    3722     189 
   Size:        201      42      33    1819    1438    1446

  Fragments arranged by size: 

                1819    1446    1438     201      42      33
 
ApaI G_GGCC'C

Cuts at:   1187    1479    1187 
   Size:        292    4687
 
ApaLI G'TGCA_C

Cuts at:   3817    3817 
   Size:       4979
 
ApoI r'AATT_y

Cuts at:   1983    4332    4588    1983 
   Size:       2349     256    2374

  Fragments arranged by size: 

                2374    2349     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4979
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4979
 
AvaI C'yCGr_G

Cuts at:    496    1180    1675    1999     496 
   Size:        684     495     324    3476

  Fragments arranged by size: 

                3476     684     495     324
 
BamHI G'GATC_C

Cuts at:   2004    2004 
   Size:       4979
 
BanI G'GyrC_C

Cuts at:    166    1487    1648    1807    1995    2409    2657    2692     166 
   Size:       1321     161     159     188     414     248      35    2453

  Fragments arranged by size: 

                2453    1321     414     248     188     161     159      35
            
 
BanII G_rGCy'C

Cuts at:    634    1187    1479    1823    1993    3023     634 
   Size:        553     292     344     170    1030    2590

  Fragments arranged by size: 

                2590    1030     553     344     292     170
 
BbeI G_GCGC'C

Cuts at:   1491    2413    2661    1491 
   Size:        922     248    3809

  Fragments arranged by size: 

                3809     922     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    557     791     802     557 
   Size:        234      11    4734

  Fragments arranged by size: 

                4734     234      11
 
BbvCI CC'TCA_GC

Cuts at:   1127    1127 
   Size:       4979
 
BciVI GTATCCnnnnn_n'

Cuts at:   2877    3933    4979    2877 
   Size:       1056    1046    2877

  Fragments arranged by size: 

                2877    1056    1046
 
BclI T'GATC_A

Cuts at:   2499    2499 
   Size:       4979
 
BglI GCCn_nnn'nGGC

Cuts at:    223     223 
   Size:       4979
 
BglII A'GATC_T

Cuts at:   1163    2494    4531    1163 
   Size:       1331    2037    1611

  Fragments arranged by size: 

                2037    1611    1331
 
BlpI GC'TnA_GC

Cuts at:    142    1010    1087    2091     142 
   Size:        868      77    1004    3030

  Fragments arranged by size: 

                3030    1004     868      77
 
Bme1580I G_kGCm'C

Cuts at:    169     219    1032    1158    1187    1479    1810    2604    2697 
   Size:         50     813     126      29     292     331     794      93

Cuts at:   2697    3821     169 
   Size:       1124    1327

  Fragments arranged by size: 

                1327    1124     813     794     331     292     126      93
                  50      29
 
BmgBI CAC'GTC

Cuts at:   1683    1683 
   Size:       4979
 
BmrI ACTGGGnnnn_n'

Cuts at:    937    2191    2272    2607    4751     937 
   Size:       1254      81     335    2144    1165

  Fragments arranged by size: 

                2144    1254    1165     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    282    1329    3346     282 
   Size:       1047    2017    1915

  Fragments arranged by size: 

                2017    1915    1047
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    211     811    1054    1180    1916    1917     211 
   Size:        600     243     126     736       1    3273

  Fragments arranged by size: 

                3273     736     600     243     126       1
 
Bpu10I CC'TnA_GC

Cuts at:   1127    1127 
   Size:       4979
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1746    2155    3034    3748    4046    1746 
   Size:        409     879     714     298    2679

  Fragments arranged by size: 

                2679     879     714     409     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1258    1610    1912    4683    1258 
   Size:        352     302    2771    1554

  Fragments arranged by size: 

                2771    1554     352     302
 
BsaAI yAC'GTr

Cuts at:   2332    2332 
   Size:       4979
 
BsaBI GATnn'nnATC

Cuts at:      6    2493    4530       6 
   Size:       2487    2037     455

  Fragments arranged by size: 

                2487    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    408     554     581     799    1488    2410    2658    3360    4856 
   Size:        146      27     218     689     922     248     702    1496

Cuts at:   4856     408 
   Size:        531

  Fragments arranged by size: 

                1496     922     702     689     531     248     218     146
                  27
 
BsaWI w'CCGG_w

Cuts at:   2168    2689    3367    3778    3925    4199    2168 
   Size:        521     678     411     147     274    2948

  Fragments arranged by size: 

                2948     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    412     442     644     674     784     814    1255    1285     412 
   Size:         30     202      30     110      30     441      30    4106

  Fragments arranged by size: 

                4106     441     202     110      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    148     218     454     514     517     583     665    1144    1369 
   Size:         70     236      60       3      66      82     479     225

Cuts at:   1369     148 
   Size:       3758

  Fragments arranged by size: 

                3758     479     236     225      82      70      66      60
                   3
 
BseYI C'CCAG_C

Cuts at:    162     321     491     633     942    1642    1889    2404    3162 
   Size:        159     170     142     309     700     247     515     758

Cuts at:   3162    3827    4235     162 
   Size:        665     408     906

  Fragments arranged by size: 

                 906     758     700     665     515     408     309     247
                 170     159     142
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    436     694     436 
   Size:        258    4721
 
BsiEI CG_ry'CG

Cuts at:    104    2521    2567    3797     104 
   Size:       2417      46    1230    1286

  Fragments arranged by size: 

                2417    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:   1993    2771    2961    3821    1993 
   Size:        778     190     860    3151

  Fragments arranged by size: 

                3151     860     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    169     219     634    1032    1158    1187    1479    1810    1823 
   Size:         50     415     398     126      29     292     331      13

Cuts at:   1823    1993    2604    2697    2771    2961    3023    3821     169 
   Size:        170     611      93      74     190      62     798    1327

  Fragments arranged by size: 

                1327     798     611     415     398     331     292     190
                 170     126      93      74      62      50      29      13
            
 
BspHI T'CATG_A

Cuts at:    524    1296    3411     524 
   Size:        772    2115    2092

  Fragments arranged by size: 

                2115    2092     772
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    390     899    2029    2243    2545    2926     390 
   Size:        509    1130     214     302     381    2443

  Fragments arranged by size: 

                2443    1130     509     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1359    3271    3325    4809    4974      50 
   Size:       1309    1912      54    1484     165      55

  Fragments arranged by size: 

                1912    1484    1309     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2891    2891 
   Size:       4979
 
BsrFI r'CCGG_y

Cuts at:    247    1484    2977    3158     247 
   Size:       1237    1493     181    2068

  Fragments arranged by size: 

                2068    1493    1237     181
 
BsrGI T'GTAC_A

Cuts at:   1271    1271 
   Size:       4979
 
BssSI C'ACGA_G

Cuts at:   3250    3958    3250 
   Size:        708    4271
 
BstAPI GCAn_nnn'nTGC

Cuts at:    390     465     516    2356     390 
   Size:         75      51    1840    3013

  Fragments arranged by size: 

                3013    1840      75      51
 
BstBI TT'CG_AA

Cuts at:   3340    3340 
   Size:       4979
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1941    1941 
   Size:       4979
 
BstYI r'GATC_y

Cuts at:      1     533     686    1037    1163    1280    2004    2471    2494 
   Size:        532     153     351     126     117     724     467      23

Cuts at:   2494    2829    3075    3381    3393    3479    4531       1 
   Size:        335     246     306      12      86    1052     449

  Fragments arranged by size: 

                1052     724     532     467     449     351     335     306
                 246     153     126     117      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    242    1765     242 
   Size:       1523    3456
 
BtgI C'CryG_G

Cuts at:    107     127     485     947    1940     107 
   Size:         20     358     462     993    3146

  Fragments arranged by size: 

                3146     993     462     358      20
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2929    3110    3273    2929 
   Size:        181     163    4635

  Fragments arranged by size: 

                4635     181     163
 
BtsI GCAGTG_nn'

Cuts at:    480    1608    1722    1782    2222     480 
   Size:       1128     114      60     440    3237

  Fragments arranged by size: 

                3237    1128     440     114      60
 
DraI TTT'AAA

Cuts at:   1978    1978 
   Size:       4979
 
DrdI GACnn_nn'nnGTC

Cuts at:   2685    4029    2685 
   Size:       1344    3635
 
EaeI y'GGCC_r

Cuts at:    110     908    1076    1202    2564    2738    3129    3156    4501 
   Size:        798     168     126    1362     174     391      27    1345

Cuts at:   4501     110 
   Size:        588

  Fragments arranged by size: 

                1362    1345     798     588     391     174     168     126
                  27
 
EagI C'GGCC_G

Cuts at:   2564    2564 
   Size:       4979
 
EarI CTCTTCn'nnn_

Cuts at:   1874    3002    3212    1874 
   Size:       1128     210    3641

  Fragments arranged by size: 

                3641    1128     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3187    3929    4075    4789    3187 
   Size:        742     146     714    3377

  Fragments arranged by size: 

                3377     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   1991    1991 
   Size:       4979
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    211     811    1054    1180    1258    1916    1917    2804    3236 
   Size:        600     243     126      78     658       1     887     432

Cuts at:   3236    3589    4251     211 
   Size:        353     662     939

  Fragments arranged by size: 

                 939     887     662     658     600     432     353     243
                 126      78       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:    644    1240     644 
   Size:        596    4383
 
EcoO109I rG'GnC_Cy

Cuts at:   1050    1475    1476    1687    2118    1050 
   Size:        425       1     211     431    3911

  Fragments arranged by size: 

                3911     431     425     211       1
 
EcoRI G'AATT_C

Cuts at:   1983    1983 
   Size:       4979
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    531    2998     531 
   Size:       2467    2512
 
FspI TGC'GCA

Cuts at:   1465    2760    1465 
   Size:       1295    3684
 
HaeII r_GCGC'y

Cuts at:    279    1383    1394    1491    2413    2661    3891    4662     279 
   Size:       1104      11      97     922     248    1230     771     596

  Fragments arranged by size: 

                1230    1104     922     771     596     248      97      11
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    784     816    2821    2851    2853    2883     784 
   Size:         32    2005      30       2      30    2880

  Fragments arranged by size: 

                2880    2005      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1452    2018    1452 
   Size:        566    4413
 
KasI G'GCGC_C

Cuts at:   1487    2409    2657    1487 
   Size:        922     248    3809

  Fragments arranged by size: 

                3809     922     248
 
KpnI G_GTAC'C

Cuts at:   1999    1999 
   Size:       4979
 
MluI A'CGCG_T

Cuts at:   4412    4412 
   Size:       4979
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    524    3738    3922     524 
   Size:       3214     184    1581

  Fragments arranged by size: 

                3214    1581     184
 
MscI TGG'CCA

Cuts at:    112     910    1078    1204    2740     112 
   Size:        798     168     126    1536    2351

  Fragments arranged by size: 

                2351    1536     798     168     126
 
MslI CAynn'nnrTG

Cuts at:    112     118    1342    2968    3095     112 
   Size:          6    1224    1626     127    1996

  Fragments arranged by size: 

                1996    1626    1224     127       6
 
MspA1I CmG'CkG

Cuts at:    129    1415    1696    1736    2090    2404    2764    3548    3793 
   Size:       1286     281      40     354     314     360     784     245

Cuts at:   3793     129 
   Size:       1315

  Fragments arranged by size: 

                1315    1286     784     360     354     314     281     245
                  40
 
NaeI GCC'GGC

Cuts at:    249    3160     249 
   Size:       2911    2068
 
NarI GG'CG_CC

Cuts at:   1488    2410    2658    1488 
   Size:        922     248    3809

  Fragments arranged by size: 

                3809     922     248
 
NcoI C'CATG_G

Cuts at:    107    1940     107 
   Size:       1833    3146
 
NgoMIV G'CCGG_C

Cuts at:    247    3158     247 
   Size:       2911    2068
 
NspI r_CATG'y

Cuts at:   2032    2532    4135    2032 
   Size:        500    1603    2876

  Fragments arranged by size: 

                2876    1603     500
 
PciI A'CATG_T

Cuts at:   4131    4131 
   Size:       4979
 
PflMI CCAn_nnn'nTGG

Cuts at:    189     189 
   Size:       4979
 
PfoI T'CCnGG_A

Cuts at:    914    1040    1754     914 
   Size:        126     714    4139

  Fragments arranged by size: 

                4139     714     126
 
PmeI GTTT'AAAC

Cuts at:   1978    1978 
   Size:       4979
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3425    3457    3425 
   Size:         32    4947
 
PpuMI rG'GwC_Cy

Cuts at:   1050    1687    1050 
   Size:        637    4342
 
PspOMI G'GGCC_C

Cuts at:   1183    1475    1183 
   Size:        292    4687
 
PspXI vC'TCGA_Gb

Cuts at:    496    1675     496 
   Size:       1179    3800
 
PstI C_TGCA'G

Cuts at:    470    1802    2026    2711     470 
   Size:       1332     224     685    2738

  Fragments arranged by size: 

                2738    1332     685     224
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4979
 
PvuII CAG'CTG

Cuts at:   1415    1696    2404    2764    1415 
   Size:        281     708     360    3630

  Fragments arranged by size: 

                3630     708     360     281
 
RsrII CG'GwC_CG

Cuts at:   3174    3174 
   Size:       4979
 
SacI G_AGCT'C

Cuts at:   1993    1993 
   Size:       4979
 
SacII CC_GC'GG

Cuts at:    130     130 
   Size:       4979
 
SalI G'TCGA_C

Cuts at:   2016    2016 
   Size:       4979
 
SapI GCTCTTCn'nnn_

Cuts at:   1874    3002    3212    1874 
   Size:       1128     210    3641

  Fragments arranged by size: 

                3641    1128     210
 
SbfI CC_TGCA'GG

Cuts at:   2026    2026 
   Size:       4979
 
ScaI AGT'ACT

Cuts at:   4496    4496 
   Size:       4979
 
SexAI A'CCwGG_T

Cuts at:    716     716 
   Size:       4979
 
SfcI C'TryA_G

Cuts at:     33     368     466    1691    1798    2022    2707    3675    3866 
   Size:        335      98    1225     107     224     685     968     191

Cuts at:   3866      33 
   Size:       1146

  Fragments arranged by size: 

                1225    1146     968     685     335     224     191     107
                  98
 
SfoI GGC'GCC

Cuts at:   1489    2411    2659    1489 
   Size:        922     248    3809

  Fragments arranged by size: 

                3809     922     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4979
 
SmaI CCC'GGG

Cuts at:   2001    2001 
   Size:       4979
 
SmlI C'TyrA_G

Cuts at:    496    1675    1725    2134    3049    3763    4025     496 
   Size:       1179      50     409     915     714     262    1450

  Fragments arranged by size: 

                1450    1179     915     714     409     262      50
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4979
 
SphI G_CATG'C

Cuts at:   2032    2532    2032 
   Size:        500    4479
 
SspI AAT'ATT

Cuts at:   1141    1141 
   Size:       4979
 
StuI AGG'CCT

Cuts at:   1426    1426 
   Size:       4979
 
StyI C'CwwG_G

Cuts at:    107     170    1574    1940    2113    2467     107 
   Size:         63    1404     366     173     354    2619

  Fragments arranged by size: 

                2619    1404     366     354     173      63
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    956    1649    2562    2695    3154    3363     956 
   Size:        693     913     133     459     209    2572

  Fragments arranged by size: 

                2572     913     693     459     209     133
 
TatI w'GTAC_w

Cuts at:    872     998    1271    4494     872 
   Size:        126     273    3223    1357

  Fragments arranged by size: 

                3223    1357     273     126
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    412     444     644     676    1022    1054    4496    4528     412 
   Size:         32     200      32     346      32    3442      32     863

  Fragments arranged by size: 

                3442     863     346     200      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   2776    2776 
   Size:       4979
 
XbaI T'CTAG_A

Cuts at:   2010    2010 
   Size:       4979
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    114     594    1218    2474     114 
   Size:        480     624    1256    2619

  Fragments arranged by size: 

                2619    1256     624     480
 
XhoI C'TCGA_G

Cuts at:    496    1675     496 
   Size:       1179    3800
 
XmaI C'CCGG_G

Cuts at:   1999    1999 
   Size:       4979
 
ZraI GAC'GTC

Cuts at:    582     800     582 
   Size:        218    4761
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI
   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI

 Enzymes that do not cut: 

    AflII     AgeI     AhdI     AleI     AscI    AvrII     BaeI     BcgI
    BfrBI     BmtI    BsiWI     BsmI    BsmBI    BspEI   BssHII   BstEII
  BstZ17I     ClaI    CspCI   DraIII    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PmlI    PshAI     PsiI     PsrI    SanDI     SfiI    SgrAI
    SnaBI     SrfI     SwaI     XmnI
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