Restriction map for
pF1KB8671
(Circular) (Six-base) MAPSORT of: pF1KB8671.seq Check: 3725 from: 1 to: 4301
pF1KB8671 4301 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 785 1339 785
Size: 554 3747
Acc65I G'GTAC_C
Cuts at: 1317 1317
Size: 4301
AclI AA'CG_TT
Cuts at: 4141 4141
Size: 4301
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 233 547 1163 2126 2558 2911 3573 233
Size: 314 616 963 432 353 662 961
Fragments arranged by size:
963 961 662 616 432 353 314
AflIII A'CryG_T
Cuts at: 478 1653 3453 3734 478
Size: 1175 1800 281 1045
Fragments arranged by size:
1800 1175 1045 281
AlwNI CAG_nnn'CTG
Cuts at: 423 1606 3044 423
Size: 1183 1438 1680
Fragments arranged by size:
1680 1438 1183
ApaI G_GGCC'C
Cuts at: 593 593
Size: 4301
ApaLI G'TGCA_C
Cuts at: 3139 3139
Size: 4301
ApoI r'AATT_y
Cuts at: 356 1264 1305 3654 3910 356
Size: 908 41 2349 256 747
Fragments arranged by size:
2349 908 747 256 41
AseI AT'TA_AT
Cuts at: 20 20
Size: 4301
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4301
AvaI C'yCGr_G
Cuts at: 1321 1321
Size: 4301
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 934 967 934
Size: 33 4268
BamHI G'GATC_C
Cuts at: 1326 1326
Size: 4301
BanI G'GyrC_C
Cuts at: 1317 1731 1979 2014 1317
Size: 414 248 35 3604
Fragments arranged by size:
3604 414 248 35
BanII G_rGCy'C
Cuts at: 408 593 1315 2345 408
Size: 185 722 1030 2364
Fragments arranged by size:
2364 1030 722 185
BbeI G_GCGC'C
Cuts at: 1735 1983 1735
Size: 248 4053
BbvCI CC'TCA_GC
Cuts at: 935 935
Size: 4301
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4267
BciVI GTATCCnnnnn_n'
Cuts at: 2199 3255 4301 2199
Size: 1056 1046 2199
Fragments arranged by size:
2199 1056 1046
BclI T'GATC_A
Cuts at: 1821 1821
Size: 4301
BglII A'GATC_T
Cuts at: 1816 3853 1816
Size: 2037 2264
BlpI GC'TnA_GC
Cuts at: 1413 1413
Size: 4301
Bme1580I G_kGCm'C
Cuts at: 593 1926 2019 3143 593
Size: 1333 93 1124 1751
Fragments arranged by size:
1751 1333 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1513 1594 1929 4073 1513
Size: 81 335 2144 1741
Fragments arranged by size:
2144 1741 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2668 2668
Size: 4301
Bpu10I CC'TnA_GC
Cuts at: 935 935
Size: 4301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 898 1477 2356 3070 3368 898
Size: 579 879 714 298 1831
Fragments arranged by size:
1831 879 714 579 298
BsaI GGTCTCn'nnnn_
Cuts at: 4005 4005
Size: 4301
BsaAI yAC'GTr
Cuts at: 481 1654 481
Size: 1173 3128
BsaBI GATnn'nnATC
Cuts at: 6 1815 3852 6
Size: 1809 2037 455
Fragments arranged by size:
2037 1809 455
BsaHI Gr'CG_yC
Cuts at: 1732 1980 2682 4178 1732
Size: 248 702 1496 1855
Fragments arranged by size:
1855 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1490 2011 2689 3100 3247 3521 1490
Size: 521 678 411 147 274 2270
Fragments arranged by size:
2270 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1071 1101 1071
Size: 30 4271
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 641 644 952 1079 641
Size: 3 308 127 3863
Fragments arranged by size:
3863 308 127 3
BseYI C'CCAG_C
Cuts at: 1000 1726 2484 3149 3557 1000
Size: 726 758 665 408 1744
Fragments arranged by size:
1744 758 726 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 255 255
Size: 4301
BsiEI CG_ry'CG
Cuts at: 104 1843 1889 3119 104
Size: 1739 46 1230 1286
Fragments arranged by size:
1739 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 408 1315 2093 2283 3143 408
Size: 907 778 190 860 1566
Fragments arranged by size:
1566 907 860 778 190
BsmI GAATG_Cn'
Cuts at: 435 435
Size: 4301
Bsp1286I G_dGCh'C
Cuts at: 408 593 1315 1926 2019 2093 2283 2345 3143
Size: 185 722 611 93 74 190 62 798
Cuts at: 3143 408
Size: 1566
Fragments arranged by size:
1566 798 722 611 190 185 93 74
62
BspHI T'CATG_A
Cuts at: 717 2733 717
Size: 2016 2285
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1351 1565 1867 2248 1351
Size: 214 302 381 3404
Fragments arranged by size:
3404 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2593 2647 4131 4296 50
Size: 2543 54 1484 165 55
Fragments arranged by size:
2543 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2213 2213
Size: 4301
BsrFI r'CCGG_y
Cuts at: 2299 2480 2299
Size: 181 4120
BssSI C'ACGA_G
Cuts at: 2572 3280 2572
Size: 708 3593
BstAPI GCAn_nnn'nTGC
Cuts at: 1678 1678
Size: 4301
BstBI TT'CG_AA
Cuts at: 2662 2662
Size: 4301
BstYI r'GATC_y
Cuts at: 1 525 1326 1793 1816 2151 2397 2703 2715
Size: 524 801 467 23 335 246 306 12
Cuts at: 2715 2801 3853 1
Size: 86 1052 449
Fragments arranged by size:
1052 801 524 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1091 1091
Size: 4301
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2251 2432 2595 2251
Size: 181 163 3957
Fragments arranged by size:
3957 181 163
BtsI GCAGTG_nn'
Cuts at: 1034 1544 1034
Size: 510 3791
DraI TTT'AAA
Cuts at: 1167 1300 1167
Size: 133 4168
DraIII CAC_nnn'GTG
Cuts at: 976 976
Size: 4301
DrdI GACnn_nn'nnGTC
Cuts at: 2007 3351 2007
Size: 1344 2957
EaeI y'GGCC_r
Cuts at: 1886 2060 2451 2478 3823 1886
Size: 174 391 27 1345 2364
Fragments arranged by size:
2364 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1886 1886
Size: 4301
EarI CTCTTCn'nnn_
Cuts at: 551 2324 2534 551
Size: 1773 210 2318
Fragments arranged by size:
2318 1773 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2509 3251 3397 4111 2509
Size: 742 146 714 2699
Fragments arranged by size:
2699 742 714 146
EcoICRI GAG'CTC
Cuts at: 406 1313 406
Size: 907 3394
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 233 547 1163 2126 2558 2911 3573 233
Size: 314 616 963 432 353 662 961
Fragments arranged by size:
963 961 662 616 432 353 314
EcoO109I rG'GnC_Cy
Cuts at: 420 902 1440 420
Size: 482 538 3281
Fragments arranged by size:
3281 538 482
EcoRI G'AATT_C
Cuts at: 1305 1305
Size: 4301
EcoRV GAT'ATC
Cuts at: 501 501
Size: 4301
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2320 2320
Size: 4301
FspI TGC'GCA
Cuts at: 2082 2082
Size: 4301
HaeII r_GCGC'y
Cuts at: 1735 1983 3213 3984 1735
Size: 248 1230 771 2052
Fragments arranged by size:
2052 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1071 1103 1238 1270 2143 2173 2175 2205 1071
Size: 32 135 32 873 30 2 30 3167
Fragments arranged by size:
3167 873 135 32 32 30 30 2
HincII GTy'rAC
Cuts at: 233 1340 233
Size: 1107 3194
HpaI GTT'AAC
Cuts at: 233 233
Size: 4301
KasI G'GCGC_C
Cuts at: 1731 1979 1731
Size: 248 4053
KpnI G_GTAC'C
Cuts at: 1321 1321
Size: 4301
MluI A'CGCG_T
Cuts at: 3734 3734
Size: 4301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 171 1041 3060 3244 171
Size: 870 2019 184 1228
Fragments arranged by size:
2019 1228 870 184
MscI TGG'CCA
Cuts at: 2062 2062
Size: 4301
MslI CAynn'nnrTG
Cuts at: 449 2290 2417 449
Size: 1841 127 2333
Fragments arranged by size:
2333 1841 127
MspA1I CmG'CkG
Cuts at: 1412 1726 2086 2870 3115 1412
Size: 314 360 784 245 2598
Fragments arranged by size:
2598 784 360 314 245
NaeI GCC'GGC
Cuts at: 2482 2482
Size: 4301
NarI GG'CG_CC
Cuts at: 1732 1980 1732
Size: 248 4053
NgoMIV G'CCGG_C
Cuts at: 2480 2480
Size: 4301
NspI r_CATG'y
Cuts at: 1354 1854 3457 1354
Size: 500 1603 2198
Fragments arranged by size:
2198 1603 500
PciI A'CATG_T
Cuts at: 3453 3453
Size: 4301
PflMI CCAn_nnn'nTGG
Cuts at: 154 205 154
Size: 51 4250
PmeI GTTT'AAAC
Cuts at: 1300 1300
Size: 4301
PmlI CAC'GTG
Cuts at: 481 481
Size: 4301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2747 2779 2747
Size: 32 4269
PspOMI G'GGCC_C
Cuts at: 589 589
Size: 4301
PstI C_TGCA'G
Cuts at: 1041 1101 1348 2033 1041
Size: 60 247 685 3309
Fragments arranged by size:
3309 685 247 60
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4301
PvuII CAG'CTG
Cuts at: 1726 2086 1726
Size: 360 3941
RsrII CG'GwC_CG
Cuts at: 2496 2496
Size: 4301
SacI G_AGCT'C
Cuts at: 408 1315 408
Size: 907 3394
SalI G'TCGA_C
Cuts at: 1338 1338
Size: 4301
SapI GCTCTTCn'nnn_
Cuts at: 551 2324 2534 551
Size: 1773 210 2318
Fragments arranged by size:
2318 1773 210
SbfI CC_TGCA'GG
Cuts at: 1348 1348
Size: 4301
ScaI AGT'ACT
Cuts at: 3818 3818
Size: 4301
SfcI C'TryA_G
Cuts at: 33 1037 1097 1344 2029 2997 3188 33
Size: 1004 60 247 685 968 191 1146
Fragments arranged by size:
1146 1004 968 685 247 191 60
SfoI GGC'GCC
Cuts at: 1733 1981 1733
Size: 248 4053
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4301
SmaI CCC'GGG
Cuts at: 1323 1323
Size: 4301
SmlI C'TyrA_G
Cuts at: 913 1456 2371 3085 3347 913
Size: 543 915 714 262 1867
Fragments arranged by size:
1867 915 714 543 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4301
SphI G_CATG'C
Cuts at: 1354 1854 1354
Size: 500 3801
StyI C'CwwG_G
Cuts at: 905 1435 1789 905
Size: 530 354 3417
Fragments arranged by size:
3417 530 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 846 1884 2017 2476 2685 846
Size: 1038 133 459 209 2462
Fragments arranged by size:
2462 1038 459 209 133
TatI w'GTAC_w
Cuts at: 1208 3816 1208
Size: 2608 1693
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 264 296 3818 3850 264
Size: 32 3522 32 715
Fragments arranged by size:
3522 715 32 32
Tth111I GACn'n_nGTC
Cuts at: 2098 2098
Size: 4301
XbaI T'CTAG_A
Cuts at: 1332 1332
Size: 4301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1796 1796
Size: 4301
XmaI C'CCGG_G
Cuts at: 1321 1321
Size: 4301
XmnI GAAnn'nnTTC
Cuts at: 560 560
Size: 4301
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PmeI PmlI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI AscI AvrII BbsI BfrBI BglI BmgBI BmtI
BpmI BsiWI BsmBI BspEI BsrGI BssHII BstEII BstXI
BstZ17I BtgI ClaI CspCI EcoNI FseI FspAI HindIII
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI