Restriction map for
pF1KB8669
(Circular) (Six-base) MAPSORT of: pF1KB8669.seq Check: 2585 from: 1 to: 4883
pF1KB8669 4883 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 867 867
Size: 4883
AccI GT'mk_AC
Cuts at: 1921 1921
Size: 4883
Acc65I G'GTAC_C
Cuts at: 782 1031 1899 782
Size: 249 868 3766
Fragments arranged by size:
3766 868 249
AclI AA'CG_TT
Cuts at: 4723 4723
Size: 4883
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1242 1573 2708 3140 3493 4155 1242
Size: 331 1135 432 353 662 1970
Fragments arranged by size:
1970 1135 662 432 353 331
AflIII A'CryG_T
Cuts at: 2235 4035 4316 2235
Size: 1800 281 2802
Fragments arranged by size:
2802 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 616 694 819 979 1077 2188 3626 616
Size: 78 125 160 98 1111 1438 1873
Fragments arranged by size:
1873 1438 1111 160 125 98 78
ApaI G_GGCC'C
Cuts at: 973 1723 973
Size: 750 4133
ApaLI G'TGCA_C
Cuts at: 1171 3721 1171
Size: 2550 2333
ApoI r'AATT_y
Cuts at: 1887 4236 4492 1887
Size: 2349 256 2278
Fragments arranged by size:
2349 2278 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4883
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4883
AvaI C'yCGr_G
Cuts at: 239 1630 1903 239
Size: 1391 273 3219
Fragments arranged by size:
3219 1391 273
AvrII C'CTAG_G
Cuts at: 405 405
Size: 4883
BamHI G'GATC_C
Cuts at: 1064 1908 1064
Size: 844 4039
BanI G'GyrC_C
Cuts at: 203 348 449 768 782 999 1031 1221 1445
Size: 145 101 319 14 217 32 190 224
Cuts at: 1445 1899 2313 2561 2596 203
Size: 454 414 248 35 2490
Fragments arranged by size:
2490 454 414 319 248 224 217 190
145 101 35 32 14
BanII G_rGCy'C
Cuts at: 584 973 1412 1655 1723 1897 2927 584
Size: 389 439 243 68 174 1030 2540
Fragments arranged by size:
2540 1030 439 389 243 174 68
BbeI G_GCGC'C
Cuts at: 1449 2317 2565 1449
Size: 868 248 3767
Fragments arranged by size:
3767 868 248
BbsI GAAGACnn'nnnn_
Cuts at: 1186 1188 1186
Size: 2 4881
BciVI GTATCCnnnnn_n'
Cuts at: 617 2781 3837 4883 617
Size: 2164 1056 1046 617
Fragments arranged by size:
2164 1056 1046 617
BclI T'GATC_A
Cuts at: 1532 2403 1532
Size: 871 4012
BglI GCCn_nnn'nGGC
Cuts at: 437 473 950 1237 1718 437
Size: 36 477 287 481 3602
Fragments arranged by size:
3602 481 477 287 36
BglII A'GATC_T
Cuts at: 1127 1398 2398 4435 1127
Size: 271 1000 2037 1575
Fragments arranged by size:
2037 1575 1000 271
BlpI GC'TnA_GC
Cuts at: 536 1654 1995 536
Size: 1118 341 3424
Fragments arranged by size:
3424 1118 341
Bme1580I G_kGCm'C
Cuts at: 147 353 452 741 973 1002 1175 1226 1691
Size: 206 99 289 232 29 173 51 465
Cuts at: 1691 1723 2508 2601 3725 147
Size: 32 785 93 1124 1305
Fragments arranged by size:
1305 1124 785 465 289 232 206 173
99 93 51 32 29
BmgBI CAC'GTC
Cuts at: 1623 1623
Size: 4883
BmrI ACTGGGnnnn_n'
Cuts at: 710 728 735 1764 2095 2176 2511 4655 710
Size: 18 7 1029 331 81 335 2144 938
Fragments arranged by size:
2144 1029 938 335 331 81 18 7
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 690 1401 3250 690
Size: 711 1849 2323
Fragments arranged by size:
2323 1849 711
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 902 1001 1348 1383 565
Size: 337 99 347 35 4065
Fragments arranged by size:
4065 347 337 99 35
Bpu10I CC'TnA_GC
Cuts at: 578 665 578
Size: 87 4796
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2059 2938 3652 3950 2059
Size: 879 714 298 2992
Fragments arranged by size:
2992 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 162 4587 162
Size: 4425 458
BsaAI yAC'GTr
Cuts at: 1518 2236 1518
Size: 718 4165
BsaBI GATnn'nnATC
Cuts at: 6 2397 4434 6
Size: 2391 2037 455
Fragments arranged by size:
2391 2037 455
BsaHI Gr'CG_yC
Cuts at: 1446 1548 2314 2562 3264 4760 1446
Size: 102 766 248 702 1496 1569
Fragments arranged by size:
1569 1496 766 702 248 102
BsaWI w'CCGG_w
Cuts at: 2072 2593 3271 3682 3829 4103 2072
Size: 521 678 411 147 274 2852
Fragments arranged by size:
2852 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 862 862
Size: 4883
BseYI C'CCAG_C
Cuts at: 344 486 637 786 809 827 838 2308 3066
Size: 142 151 149 23 18 11 1470 758
Cuts at: 3066 3731 4139 344
Size: 665 408 1088
Fragments arranged by size:
1470 1088 758 665 408 151 149 142
23 18 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1237 1237
Size: 4883
BsiEI CG_ry'CG
Cuts at: 104 1442 1746 2425 2471 3701 104
Size: 1338 304 679 46 1230 1286
Fragments arranged by size:
1338 1286 1230 679 304 46
BsiHKAI G_wGCw'C
Cuts at: 1175 1412 1754 1796 1897 2675 2865 3725 1175
Size: 237 342 42 101 778 190 860 2333
Fragments arranged by size:
2333 860 778 342 237 190 101 42
BsmBI CGTCTCn'nnnn_
Cuts at: 1198 1198
Size: 4883
Bsp1286I G_dGCh'C
Cuts at: 147 353 452 584 741 973 1002 1175 1226
Size: 206 99 132 157 232 29 173 51
Cuts at: 1226 1412 1655 1691 1723 1754 1796 1897 2508
Size: 186 243 36 32 31 42 101 611
Cuts at: 2508 2601 2675 2865 2927 3725 147
Size: 93 74 190 62 798 1305
Fragments arranged by size:
1305 798 611 243 232 206 190 186
173 157 132 101 99 93 74 62
51 42 36 32 31 29
BspHI T'CATG_A
Cuts at: 3315 3315
Size: 4883
BspMI ACCTGCnnnn'nnnn_
Cuts at: 744 867 1142 1933 2147 2449 2830 744
Size: 123 275 791 214 302 381 2797
Fragments arranged by size:
2797 791 381 302 275 214 123
BsrBI CCG'CTC
Cuts at: 50 962 1359 3175 3229 4713 4878 50
Size: 912 397 1816 54 1484 165 55
Fragments arranged by size:
1816 1484 912 397 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2795 2795
Size: 4883
BsrFI r'CCGG_y
Cuts at: 137 953 2881 3062 137
Size: 816 1928 181 1958
Fragments arranged by size:
1958 1928 816 181
BssSI C'ACGA_G
Cuts at: 3154 3862 3154
Size: 708 4175
BstAPI GCAn_nnn'nTGC
Cuts at: 2260 2260
Size: 4883
BstBI TT'CG_AA
Cuts at: 3244 3244
Size: 4883
BstXI CCAn_nnnn'nTGG
Cuts at: 940 940
Size: 4883
BstYI r'GATC_y
Cuts at: 1 1064 1127 1398 1908 2375 2398 2733 2979
Size: 1063 63 271 510 467 23 335 246
Cuts at: 2979 3285 3297 3383 4435 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1063 1052 510 467 449 335 306 271
246 86 63 23 12
BtgI C'CryG_G
Cuts at: 1436 1664 1787 1436
Size: 228 123 4532
Fragments arranged by size:
4532 228 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1501 1644 2833 3014 3177 1501
Size: 143 1189 181 163 3207
Fragments arranged by size:
3207 1189 181 163 143
BtsI GCAGTG_nn'
Cuts at: 383 635 849 2126 383
Size: 252 214 1277 3140
Fragments arranged by size:
3140 1277 252 214
DraI TTT'AAA
Cuts at: 1882 1882
Size: 4883
DraIII CAC_nnn'GTG
Cuts at: 1006 1006
Size: 4883
DrdI GACnn_nn'nnGTC
Cuts at: 1120 2589 3933 1120
Size: 1469 1344 2070
Fragments arranged by size:
2070 1469 1344
EaeI y'GGCC_r
Cuts at: 474 632 951 1008 1229 1302 1334 1439 1743
Size: 158 319 57 221 73 32 105 304
Cuts at: 1743 1769 2468 2642 3033 3060 4405 474
Size: 26 699 174 391 27 1345 952
Fragments arranged by size:
1345 952 699 391 319 304 221 174
158 105 73 57 32 27 26
EagI C'GGCC_G
Cuts at: 1439 1743 2468 1439
Size: 304 725 3854
Fragments arranged by size:
3854 725 304
EarI CTCTTCn'nnn_
Cuts at: 328 2906 3116 328
Size: 2578 210 2095
Fragments arranged by size:
2578 2095 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 455 972 3091 3833 3979 4693 455
Size: 517 2119 742 146 714 645
Fragments arranged by size:
2119 742 714 645 517 146
EcoICRI GAG'CTC
Cuts at: 1410 1895 1410
Size: 485 4398
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 902 1001 1242 1348 1383 1573 2708 3140
Size: 337 99 241 106 35 190 1135 432
Cuts at: 3140 3493 4155 565
Size: 353 662 1293
Fragments arranged by size:
1293 1135 662 432 353 337 241 190
106 99 35
EcoO109I rG'GnC_Cy
Cuts at: 409 969 1074 1720 2022 409
Size: 560 105 646 302 3270
Fragments arranged by size:
3270 646 560 302 105
EcoRI G'AATT_C
Cuts at: 1887 1887
Size: 4883
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1417 2902 1417
Size: 1485 3398
FspI TGC'GCA
Cuts at: 397 2664 397
Size: 2267 2616
HaeII r_GCGC'y
Cuts at: 855 1449 1612 2317 2565 3795 4566 855
Size: 594 163 705 248 1230 771 1172
Fragments arranged by size:
1230 1172 771 705 594 248 163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 586 618 1267 1299 2725 2755 2757 2787 586
Size: 32 649 32 1426 30 2 30 2682
Fragments arranged by size:
2682 1426 649 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1922 1922
Size: 4883
KasI G'GCGC_C
Cuts at: 1445 2313 2561 1445
Size: 868 248 3767
Fragments arranged by size:
3767 868 248
KpnI G_GTAC'C
Cuts at: 786 1035 1903 786
Size: 249 868 3766
Fragments arranged by size:
3766 868 249
MluI A'CGCG_T
Cuts at: 4316 4316
Size: 4883
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1124 3642 3826 1124
Size: 2518 184 2181
Fragments arranged by size:
2518 2181 184
MscI TGG'CCA
Cuts at: 476 1771 2644 476
Size: 1295 873 2715
Fragments arranged by size:
2715 1295 873
MslI CAynn'nnrTG
Cuts at: 262 503 509 1663 2872 2999 262
Size: 241 6 1154 1209 127 2146
Fragments arranged by size:
2146 1209 1154 241 127 6
MspA1I CmG'CkG
Cuts at: 637 1053 1994 2308 2668 3452 3697 637
Size: 416 941 314 360 784 245 1823
Fragments arranged by size:
1823 941 784 416 360 314 245
NaeI GCC'GGC
Cuts at: 139 955 3064 139
Size: 816 2109 1958
Fragments arranged by size:
2109 1958 816
NarI GG'CG_CC
Cuts at: 1446 2314 2562 1446
Size: 868 248 3767
Fragments arranged by size:
3767 868 248
NgoMIV G'CCGG_C
Cuts at: 137 953 3062 137
Size: 816 2109 1958
Fragments arranged by size:
2109 1958 816
NspI r_CATG'y
Cuts at: 1936 2436 4039 1936
Size: 500 1603 2780
Fragments arranged by size:
2780 1603 500
PciI A'CATG_T
Cuts at: 4035 4035
Size: 4883
PflMI CCAn_nnn'nTGG
Cuts at: 979 1056 979
Size: 77 4806
PfoI T'CCnGG_A
Cuts at: 331 1325 331
Size: 994 3889
PmeI GTTT'AAAC
Cuts at: 1882 1882
Size: 4883
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3329 3361 3329
Size: 32 4851
PspOMI G'GGCC_C
Cuts at: 969 1719 969
Size: 750 4133
PstI C_TGCA'G
Cuts at: 621 1073 1930 2615 621
Size: 452 857 685 2889
Fragments arranged by size:
2889 857 685 452
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4883
PvuII CAG'CTG
Cuts at: 1053 2308 2668 1053
Size: 1255 360 3268
Fragments arranged by size:
3268 1255 360
RsrII CG'GwC_CG
Cuts at: 3078 3078
Size: 4883
SacI G_AGCT'C
Cuts at: 1412 1897 1412
Size: 485 4398
SalI G'TCGA_C
Cuts at: 1920 1920
Size: 4883
SapI GCTCTTCn'nnn_
Cuts at: 2906 3116 2906
Size: 210 4673
SbfI CC_TGCA'GG
Cuts at: 1073 1930 1073
Size: 857 4026
ScaI AGT'ACT
Cuts at: 4400 4400
Size: 4883
SexAI A'CCwGG_T
Cuts at: 778 778
Size: 4883
SfcI C'TryA_G
Cuts at: 33 617 1069 1926 2611 3579 3770 33
Size: 584 452 857 685 968 191 1146
Fragments arranged by size:
1146 968 857 685 584 452 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 473 950 1237 473
Size: 477 287 4119
Fragments arranged by size:
4119 477 287
SfoI GGC'GCC
Cuts at: 1447 2315 2563 1447
Size: 868 248 3767
Fragments arranged by size:
3767 868 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4883
SmaI CCC'GGG
Cuts at: 241 1905 241
Size: 1664 3219
SmlI C'TyrA_G
Cuts at: 2038 2953 3667 3929 2038
Size: 915 714 262 2992
Fragments arranged by size:
2992 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4883
SphI G_CATG'C
Cuts at: 1936 2436 1936
Size: 500 4383
StuI AGG'CCT
Cuts at: 431 1525 431
Size: 1094 3789
StyI C'CwwG_G
Cuts at: 405 1091 1166 2017 2371 405
Size: 686 75 851 354 2917
Fragments arranged by size:
2917 851 686 354 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 236 267 301 336 460 786 823 2466 2599
Size: 31 34 35 124 326 37 1643 133
Cuts at: 2599 3058 3267 236
Size: 459 209 1852
Fragments arranged by size:
1852 1643 459 326 209 133 124 37
35 34 31
TatI w'GTAC_w
Cuts at: 4398 4398
Size: 4883
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4400 4432 4400
Size: 32 4851
Tth111I GACn'n_nGTC
Cuts at: 2680 2680
Size: 4883
XbaI T'CTAG_A
Cuts at: 1914 1914
Size: 4883
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 460 2378 460
Size: 1918 2965
XmaI C'CCGG_G
Cuts at: 239 1903 239
Size: 1664 3219
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI AloI
AscI BaeI BbvCI BcgI BfrBI BmtI BsaXI BsiWI
BsmI BspEI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI