Restriction map for
pF1KB8652
(Circular) (Six-base) MAPSORT of: pF1KB8652.seq Check: 924 from: 1 to: 3716
pF1KB8652 3716 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AatII G_ACGT'C
Cuts at: 332 332
Size: 3716
AccI GT'mk_AC
Cuts at: 754 754
Size: 3716
Acc65I G'GTAC_C
Cuts at: 732 732
Size: 3716
AclI AA'CG_TT
Cuts at: 3556 3556
Size: 3716
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 415 457 1541 1973 2720 2988 361
Size: 54 42 1084 432 747 268 1089
Fragments arranged by size:
1089 1084 747 432 268 54 42
AflIII A'CryG_T
Cuts at: 1068 2172 3149 1068
Size: 1104 977 1635
Fragments arranged by size:
1635 1104 977
AgeI A'CCGG_T
Cuts at: 2104 2104
Size: 3716
AlwNI CAG_nnn'CTG
Cuts at: 1021 2588 1021
Size: 1567 2149
ApaLI G'TGCA_C
Cuts at: 2486 2486
Size: 3716
ApoI r'AATT_y
Cuts at: 556 720 3069 3325 556
Size: 164 2349 256 947
Fragments arranged by size:
2349 947 256 164
AseI AT'TA_AT
Cuts at: 20 20
Size: 3716
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3716
AvaI C'yCGr_G
Cuts at: 736 736
Size: 3716
BamHI G'GATC_C
Cuts at: 741 741
Size: 3716
BanI G'GyrC_C
Cuts at: 732 1146 1394 1429 732
Size: 414 248 35 3019
Fragments arranged by size:
3019 414 248 35
BanII G_rGCy'C
Cuts at: 730 1760 730
Size: 1030 2686
BbeI G_GCGC'C
Cuts at: 1150 1398 1150
Size: 248 3468
BbvCI CC'TCA_GC
Cuts at: 698 698
Size: 3716
BciVI GTATCCnnnnn_n'
Cuts at: 1614 2375 3716 1614
Size: 761 1341 1614
Fragments arranged by size:
1614 1341 761
BclI T'GATC_A
Cuts at: 630 1236 630
Size: 606 3110
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3716
BglII A'GATC_T
Cuts at: 398 1231 3268 398
Size: 833 2037 846
Fragments arranged by size:
2037 846 833
BlpI GC'TnA_GC
Cuts at: 828 828
Size: 3716
Bme1580I G_kGCm'C
Cuts at: 505 1341 1434 2490 505
Size: 836 93 1056 1731
Fragments arranged by size:
1731 1056 836 93
BmrI ACTGGGnnnn_n'
Cuts at: 928 1009 1344 3488 928
Size: 81 335 2144 1156
Fragments arranged by size:
2144 1156 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 3716
Bpu10I CC'TnA_GC
Cuts at: 209 698 209
Size: 489 3227
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 892 1771 2263 2561 892
Size: 879 492 298 2047
Fragments arranged by size:
2047 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 676 3420 676
Size: 2744 972
BsaAI yAC'GTr
Cuts at: 1069 1069
Size: 3716
BsaBI GATnn'nnATC
Cuts at: 6 1230 3267 6
Size: 1224 2037 455
Fragments arranged by size:
2037 1224 455
BsaHI Gr'CG_yC
Cuts at: 329 1147 1395 2097 3593 329
Size: 818 248 702 1496 452
Fragments arranged by size:
1496 818 702 452 248
BsaWI w'CCGG_w
Cuts at: 905 1426 2104 2378 2525 2936 905
Size: 521 678 274 147 411 1685
Fragments arranged by size:
1685 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 361 694 715 361
Size: 333 21 3362
Fragments arranged by size:
3362 333 21
BseYI C'CCAG_C
Cuts at: 1141 1899 2476 2972 1141
Size: 758 577 496 1885
Fragments arranged by size:
1885 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 116 459 1258 1304 2512 104
Size: 12 343 799 46 1208 1308
Fragments arranged by size:
1308 1208 799 343 46 12
BsiHKAI G_wGCw'C
Cuts at: 730 1508 1698 2490 730
Size: 778 190 792 1956
Fragments arranged by size:
1956 792 778 190
BsmI GAATG_Cn'
Cuts at: 543 552 583 543
Size: 9 31 3676
Fragments arranged by size:
3676 31 9
Bsp1286I G_dGCh'C
Cuts at: 505 730 1341 1434 1508 1698 1760 2490 505
Size: 225 611 93 74 190 62 730 1731
Fragments arranged by size:
1731 730 611 225 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2936 2936
Size: 3716
BspHI T'CATG_A
Cuts at: 129 2892 129
Size: 2763 953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 439 766 980 1282 1663 439
Size: 327 214 302 381 2492
Fragments arranged by size:
2492 381 327 302 214
BsrBI CCG'CTC
Cuts at: 50 2008 2062 3546 3711 50
Size: 1958 54 1484 165 55
Fragments arranged by size:
1958 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1628 1628
Size: 3716
BsrFI r'CCGG_y
Cuts at: 1714 1895 2104 1714
Size: 181 209 3326
Fragments arranged by size:
3326 209 181
BssSI C'ACGA_G
Cuts at: 1987 2345 1987
Size: 358 3358
BstAPI GCAn_nnn'nTGC
Cuts at: 1093 1093
Size: 3716
BstBI TT'CG_AA
Cuts at: 2077 2077
Size: 3716
BstYI r'GATC_y
Cuts at: 1 398 741 1208 1231 1566 1812 2824 2910
Size: 397 343 467 23 335 246 1012 86
Cuts at: 2910 2922 3268 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 397 346 343 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3716
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1666 1847 2010 1666
Size: 181 163 3372
Fragments arranged by size:
3372 181 163
BtsI GCAGTG_nn'
Cuts at: 959 959
Size: 3716
DraI TTT'AAA
Cuts at: 255 715 255
Size: 460 3256
DrdI GACnn_nn'nnGTC
Cuts at: 1422 2280 1422
Size: 858 2858
EaeI y'GGCC_r
Cuts at: 113 521 1301 1475 1866 1893 3238 113
Size: 408 780 174 391 27 1345 591
Fragments arranged by size:
1345 780 591 408 391 174 27
EagI C'GGCC_G
Cuts at: 113 1301 113
Size: 1188 2528
EarI CTCTTCn'nnn_
Cuts at: 209 236 590 1739 1949 209
Size: 27 354 1149 210 1976
Fragments arranged by size:
1976 1149 354 210 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 355 664 1924 2234 2380 3526 355
Size: 309 1260 310 146 1146 545
Fragments arranged by size:
1260 1146 545 310 309 146
EcoICRI GAG'CTC
Cuts at: 728 728
Size: 3716
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 415 457 1541 1973 2720 2988 361
Size: 54 42 1084 432 747 268 1089
Fragments arranged by size:
1089 1084 747 432 268 54 42
EcoO109I rG'GnC_Cy
Cuts at: 855 855
Size: 3716
EcoRI G'AATT_C
Cuts at: 720 720
Size: 3716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 265 1735 265
Size: 1470 2246
FspI TGC'GCA
Cuts at: 1497 1497
Size: 3716
HaeII r_GCGC'y
Cuts at: 1150 1398 2420 3399 1150
Size: 248 1022 979 1467
Fragments arranged by size:
1467 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1558 1588 1590 1620 1558
Size: 30 2 30 3654
Fragments arranged by size:
3654 30 30 2
HincII GTy'rAC
Cuts at: 755 755
Size: 3716
KasI G'GCGC_C
Cuts at: 1146 1394 1146
Size: 248 3468
KpnI G_GTAC'C
Cuts at: 736 736
Size: 3716
MluI A'CGCG_T
Cuts at: 3149 3149
Size: 3716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 194 2387 2571 194
Size: 2193 184 1339
Fragments arranged by size:
2193 1339 184
MscI TGG'CCA
Cuts at: 523 1477 523
Size: 954 2762
MslI CAynn'nnrTG
Cuts at: 424 1705 1832 424
Size: 1281 127 2308
Fragments arranged by size:
2308 1281 127
MspA1I CmG'CkG
Cuts at: 827 1141 1501 2514 2759 827
Size: 314 360 1013 245 1784
Fragments arranged by size:
1784 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1897 1897
Size: 3716
NarI GG'CG_CC
Cuts at: 1147 1395 1147
Size: 248 3468
NcoI C'CATG_G
Cuts at: 107 107
Size: 3716
NgoMIV G'CCGG_C
Cuts at: 1895 1895
Size: 3716
NotI GC'GGCC_GC
Cuts at: 113 113
Size: 3716
NspI r_CATG'y
Cuts at: 769 1269 2176 769
Size: 500 907 2309
Fragments arranged by size:
2309 907 500
PciI A'CATG_T
Cuts at: 2172 2172
Size: 3716
PmeI GTTT'AAAC
Cuts at: 715 715
Size: 3716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2855 2887 2855
Size: 32 3684
PstI C_TGCA'G
Cuts at: 763 1448 763
Size: 685 3031
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3716
PvuII CAG'CTG
Cuts at: 1141 1501 1141
Size: 360 3356
RsrII CG'GwC_CG
Cuts at: 1911 1911
Size: 3716
SacI G_AGCT'C
Cuts at: 730 730
Size: 3716
SalI G'TCGA_C
Cuts at: 753 753
Size: 3716
SapI GCTCTTCn'nnn_
Cuts at: 1739 1949 1739
Size: 210 3506
SbfI CC_TGCA'GG
Cuts at: 763 763
Size: 3716
ScaI AGT'ACT
Cuts at: 3233 3233
Size: 3716
SfcI C'TryA_G
Cuts at: 33 759 1444 2437 2628 33
Size: 726 685 993 191 1121
Fragments arranged by size:
1121 993 726 685 191
SfoI GGC'GCC
Cuts at: 1148 1396 1148
Size: 248 3468
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3716
SmaI CCC'GGG
Cuts at: 738 738
Size: 3716
SmlI C'TyrA_G
Cuts at: 871 1786 2278 2540 871
Size: 915 492 262 2047
Fragments arranged by size:
2047 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3716
SphI G_CATG'C
Cuts at: 769 1269 769
Size: 500 3216
StyI C'CwwG_G
Cuts at: 107 119 686 850 1204 107
Size: 12 567 164 354 2619
Fragments arranged by size:
2619 567 354 164 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 493 1299 1432 1891 2100 493
Size: 806 133 459 209 2109
Fragments arranged by size:
2109 806 459 209 133
TatI w'GTAC_w
Cuts at: 3231 3231
Size: 3716
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3233 3265 3233
Size: 32 3684
Tth111I GACn'n_nGTC
Cuts at: 1513 1513
Size: 3716
XbaI T'CTAG_A
Cuts at: 747 747
Size: 3716
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1211 1211
Size: 3716
XmaI C'CCGG_G
Cuts at: 736 736
Size: 3716
ZraI GAC'GTC
Cuts at: 330 330
Size: 3716
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI
BpmI BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI