Restriction map for
pF1KB8646
(Circular) (Six-base) MAPSORT of: pF1KB8646.seq Check: 7795 from: 1 to: 3830
pF1KB8646 3830 bp
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AatII G_ACGT'C
Cuts at: 127 127
Size: 3830
AccI GT'mk_AC
Cuts at: 868 868
Size: 3830
Acc65I G'GTAC_C
Cuts at: 846 846
Size: 3830
AclI AA'CG_TT
Cuts at: 3670 3670
Size: 3830
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1655 2087 2834 3102 1655
Size: 432 747 268 2383
Fragments arranged by size:
2383 747 432 268
AflIII A'CryG_T
Cuts at: 1182 2286 3263 1182
Size: 1104 977 1749
Fragments arranged by size:
1749 1104 977
AgeI A'CCGG_T
Cuts at: 2218 2218
Size: 3830
AlwNI CAG_nnn'CTG
Cuts at: 1135 2702 1135
Size: 1567 2263
ApaLI G'TGCA_C
Cuts at: 2600 2600
Size: 3830
ApoI r'AATT_y
Cuts at: 834 3183 3439 834
Size: 2349 256 1225
Fragments arranged by size:
2349 1225 256
AscI GG'CGCG_CC
Cuts at: 663 663
Size: 3830
AseI AT'TA_AT
Cuts at: 20 20
Size: 3830
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3830
AvaI C'yCGr_G
Cuts at: 327 412 498 809 850 327
Size: 85 86 311 41 3307
Fragments arranged by size:
3307 311 86 85 41
BamHI G'GATC_C
Cuts at: 323 855 323
Size: 532 3298
BanI G'GyrC_C
Cuts at: 846 1260 1508 1543 846
Size: 414 248 35 3133
Fragments arranged by size:
3133 414 248 35
BanII G_rGCy'C
Cuts at: 336 419 844 1874 336
Size: 83 425 1030 2292
Fragments arranged by size:
2292 1030 425 83
BbeI G_GCGC'C
Cuts at: 1264 1512 1264
Size: 248 3582
BbsI GAAGACnn'nnnn_
Cuts at: 685 685
Size: 3830
BbvCI CC'TCA_GC
Cuts at: 223 223
Size: 3830
BciVI GTATCCnnnnn_n'
Cuts at: 1728 2489 3830 1728
Size: 761 1341 1728
Fragments arranged by size:
1728 1341 761
BclI T'GATC_A
Cuts at: 1350 1350
Size: 3830
BglII A'GATC_T
Cuts at: 1345 3382 1345
Size: 2037 1793
BlpI GC'TnA_GC
Cuts at: 942 942
Size: 3830
Bme1580I G_kGCm'C
Cuts at: 748 1455 1548 2604 748
Size: 707 93 1056 1974
Fragments arranged by size:
1974 1056 707 93
BmrI ACTGGGnnnn_n'
Cuts at: 551 754 1042 1123 1458 3602 551
Size: 203 288 81 335 2144 779
Fragments arranged by size:
2144 779 335 288 203 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 3830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 298
Size: 3830
Bpu10I CC'TnA_GC
Cuts at: 223 223
Size: 3830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1006 1885 2377 2675 1006
Size: 879 492 298 2161
Fragments arranged by size:
2161 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3534 3534
Size: 3830
BsaAI yAC'GTr
Cuts at: 1183 1183
Size: 3830
BsaBI GATnn'nnATC
Cuts at: 6 1344 3381 6
Size: 1338 2037 455
Fragments arranged by size:
2037 1338 455
BsaHI Gr'CG_yC
Cuts at: 124 1261 1509 2211 3707 124
Size: 1137 248 702 1496 247
Fragments arranged by size:
1496 1137 702 248 247
BsaWI w'CCGG_w
Cuts at: 1019 1540 2218 2492 2639 3050 1019
Size: 521 678 274 147 411 1799
Fragments arranged by size:
1799 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 381 411 381
Size: 30 3800
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 348 348
Size: 3830
BseYI C'CCAG_C
Cuts at: 141 1255 2013 2590 3086 141
Size: 1114 758 577 496 885
Fragments arranged by size:
1114 885 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 133
Size: 3830
BsiEI CG_ry'CG
Cuts at: 104 1372 1418 2626 104
Size: 1268 46 1208 1308
Fragments arranged by size:
1308 1268 1208 46
BsiHKAI G_wGCw'C
Cuts at: 816 844 1622 1812 2604 816
Size: 28 778 190 792 2042
Fragments arranged by size:
2042 792 778 190 28
BsmI GAATG_Cn'
Cuts at: 168 168
Size: 3830
BsmBI CGTCTCn'nnnn_
Cuts at: 161 161
Size: 3830
Bsp1286I G_dGCh'C
Cuts at: 336 419 748 816 844 1455 1548 1622 1812
Size: 83 329 68 28 611 93 74 190
Cuts at: 1812 1874 2604 336
Size: 62 730 1562
Fragments arranged by size:
1562 730 611 329 190 93 83 74
68 62 28
BspEI T'CCGG_A
Cuts at: 3050 3050
Size: 3830
BspHI T'CATG_A
Cuts at: 473 3006 473
Size: 2533 1297
BspMI ACCTGCnnnn'nnnn_
Cuts at: 880 1094 1396 1777 880
Size: 214 302 381 2933
Fragments arranged by size:
2933 381 302 214
BsrBI CCG'CTC
Cuts at: 50 648 2122 2176 3660 3825 50
Size: 598 1474 54 1484 165 55
Fragments arranged by size:
1484 1474 598 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1742 1742
Size: 3830
BsrFI r'CCGG_y
Cuts at: 437 1828 2009 2218 437
Size: 1391 181 209 2049
Fragments arranged by size:
2049 1391 209 181
BssHII G'CGCG_C
Cuts at: 663 663
Size: 3830
BssSI C'ACGA_G
Cuts at: 2101 2459 2101
Size: 358 3472
BstAPI GCAn_nnn'nTGC
Cuts at: 1207 1207
Size: 3830
BstBI TT'CG_AA
Cuts at: 2191 2191
Size: 3830
BstXI CCAn_nnnn'nTGG
Cuts at: 276 276
Size: 3830
BstYI r'GATC_y
Cuts at: 1 323 855 1322 1345 1680 1926 2938 3024
Size: 322 532 467 23 335 246 1012 86
Cuts at: 3024 3036 3382 1
Size: 12 346 449
Fragments arranged by size:
1012 532 467 449 346 335 322 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3830
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1780 1961 2124 1780
Size: 181 163 3486
Fragments arranged by size:
3486 181 163
BtsI GCAGTG_nn'
Cuts at: 1073 1073
Size: 3830
DraI TTT'AAA
Cuts at: 829 829
Size: 3830
DrdI GACnn_nn'nnGTC
Cuts at: 1536 2394 1536
Size: 858 2972
EaeI y'GGCC_r
Cuts at: 1415 1589 1980 2007 3352 1415
Size: 174 391 27 1345 1893
Fragments arranged by size:
1893 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1415 1415
Size: 3830
EarI CTCTTCn'nnn_
Cuts at: 1853 2063 1853
Size: 210 3620
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 265 565 2038 2348 2494 3640 265
Size: 300 1473 310 146 1146 455
Fragments arranged by size:
1473 1146 455 310 300 146
EcoICRI GAG'CTC
Cuts at: 842 842
Size: 3830
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 1655 2087 2834 3102 298
Size: 1357 432 747 268 1026
Fragments arranged by size:
1357 1026 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 693 693
Size: 3830
EcoO109I rG'GnC_Cy
Cuts at: 429 751 969 429
Size: 322 218 3290
Fragments arranged by size:
3290 322 218
EcoRI G'AATT_C
Cuts at: 834 834
Size: 3830
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1849 1849
Size: 3830
FspI TGC'GCA
Cuts at: 595 1611 595
Size: 1016 2814
HaeII r_GCGC'y
Cuts at: 223 796 1264 1512 2534 3513 223
Size: 573 468 248 1022 979 540
Fragments arranged by size:
1022 979 573 540 468 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 381 413 461 493 1672 1702 1704 1734 381
Size: 32 48 32 1179 30 2 30 2477
Fragments arranged by size:
2477 1179 48 32 32 30 30 2
HincII GTy'rAC
Cuts at: 869 869
Size: 3830
KasI G'GCGC_C
Cuts at: 1260 1508 1260
Size: 248 3582
KpnI G_GTAC'C
Cuts at: 850 850
Size: 3830
MluI A'CGCG_T
Cuts at: 3263 3263
Size: 3830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 424 800 2501 2685 424
Size: 376 1701 184 1569
Fragments arranged by size:
1701 1569 376 184
MscI TGG'CCA
Cuts at: 1591 1591
Size: 3830
MslI CAynn'nnrTG
Cuts at: 271 1819 1946 271
Size: 1548 127 2155
Fragments arranged by size:
2155 1548 127
MspA1I CmG'CkG
Cuts at: 732 941 1255 1615 2628 2873 732
Size: 209 314 360 1013 245 1689
Fragments arranged by size:
1689 1013 360 314 245 209
NaeI GCC'GGC
Cuts at: 2011 2011
Size: 3830
NarI GG'CG_CC
Cuts at: 1261 1509 1261
Size: 248 3582
NcoI C'CATG_G
Cuts at: 107 107
Size: 3830
NgoMIV G'CCGG_C
Cuts at: 2009 2009
Size: 3830
NspI r_CATG'y
Cuts at: 276 576 658 883 1383 2290 276
Size: 300 82 225 500 907 1816
Fragments arranged by size:
1816 907 500 300 225 82
PciI A'CATG_T
Cuts at: 2286 2286
Size: 3830
PmeI GTTT'AAAC
Cuts at: 829 829
Size: 3830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2969 3001 2969
Size: 32 3798
PpuMI rG'GwC_Cy
Cuts at: 429 429
Size: 3830
PstI C_TGCA'G
Cuts at: 341 877 1562 341
Size: 536 685 2609
Fragments arranged by size:
2609 685 536
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3830
PvuII CAG'CTG
Cuts at: 732 1255 1615 732
Size: 523 360 2947
Fragments arranged by size:
2947 523 360
RsrII CG'GwC_CG
Cuts at: 2025 2025
Size: 3830
SacI G_AGCT'C
Cuts at: 844 844
Size: 3830
SalI G'TCGA_C
Cuts at: 867 867
Size: 3830
SanDI GG'GwC_CC
Cuts at: 429 429
Size: 3830
SapI GCTCTTCn'nnn_
Cuts at: 1853 2063 1853
Size: 210 3620
SbfI CC_TGCA'GG
Cuts at: 877 877
Size: 3830
ScaI AGT'ACT
Cuts at: 3347 3347
Size: 3830
SfcI C'TryA_G
Cuts at: 33 337 873 1558 2551 2742 33
Size: 304 536 685 993 191 1121
Fragments arranged by size:
1121 993 685 536 304 191
SfoI GGC'GCC
Cuts at: 1262 1510 1262
Size: 248 3582
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3830
SmaI CCC'GGG
Cuts at: 329 500 852 329
Size: 171 352 3307
Fragments arranged by size:
3307 352 171
SmlI C'TyrA_G
Cuts at: 985 1900 2392 2654 985
Size: 915 492 262 2161
Fragments arranged by size:
2161 915 492 262
SpeI A'CTAG_T
Cuts at: 67 784 67
Size: 717 3113
SphI G_CATG'C
Cuts at: 658 883 1383 658
Size: 225 500 3105
Fragments arranged by size:
3105 500 225
StuI AGG'CCT
Cuts at: 487 487
Size: 3830
StyI C'CwwG_G
Cuts at: 107 146 964 1318 107
Size: 39 818 354 2619
Fragments arranged by size:
2619 818 354 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1413 1546 2005 2214 1413
Size: 133 459 209 3029
Fragments arranged by size:
3029 459 209 133
TatI w'GTAC_w
Cuts at: 3345 3345
Size: 3830
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3347 3379 3347
Size: 32 3798
Tth111I GACn'n_nGTC
Cuts at: 1627 1627
Size: 3830
XbaI T'CTAG_A
Cuts at: 861 861
Size: 3830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1325 1325
Size: 3830
XmaI C'CCGG_G
Cuts at: 327 498 850 327
Size: 171 352 3307
Fragments arranged by size:
3307 352 171
ZraI GAC'GTC
Cuts at: 125 125
Size: 3830
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsiWI
BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI