Restriction map for
pF1KB8624
(Circular) (Six-base) MAPSORT of: pF1KB8624.seq Check: 6197 from: 1 to: 4163
pF1KB8624 4163 bp
With 182 enzymes: SgfI *
July 2, 2008 13:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 372 547 685 747 372
Size: 175 138 62 3788
Fragments arranged by size:
3788 175 138 62
AatII G_ACGT'C
Cuts at: 628 628
Size: 4163
AccI GT'mk_AC
Cuts at: 293 1201 293
Size: 908 3255
Acc65I G'GTAC_C
Cuts at: 1179 1179
Size: 4163
AclI AA'CG_TT
Cuts at: 4003 4003
Size: 4163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 344 417 1988 2420 2773 3435 344
Size: 73 1571 432 353 662 1072
Fragments arranged by size:
1571 1072 662 432 353 73
AflIII A'CryG_T
Cuts at: 1155 1515 3315 3596 1155
Size: 360 1800 281 1722
Fragments arranged by size:
1800 1722 360 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 325 357 325
Size: 32 4131
AlwNI CAG_nnn'CTG
Cuts at: 276 319 930 1468 2906 276
Size: 43 611 538 1438 1533
Fragments arranged by size:
1533 1438 611 538 43
ApaI G_GGCC'C
Cuts at: 919 1036 919
Size: 117 4046
ApaLI G'TGCA_C
Cuts at: 3001 3001
Size: 4163
ApoI r'AATT_y
Cuts at: 1167 3516 3772 1167
Size: 2349 256 1558
Fragments arranged by size:
2349 1558 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4163
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
AvaI C'yCGr_G
Cuts at: 1183 1183
Size: 4163
BamHI G'GATC_C
Cuts at: 1013 1188 1013
Size: 175 3988
BanI G'GyrC_C
Cuts at: 123 136 649 673 943 1179 1593 1841 1876
Size: 13 513 24 270 236 414 248 35
Cuts at: 1876 123
Size: 2410
Fragments arranged by size:
2410 513 414 270 248 236 35 24
13
BanII G_rGCy'C
Cuts at: 919 1036 1177 2207 919
Size: 117 141 1030 2875
Fragments arranged by size:
2875 1030 141 117
BbeI G_GCGC'C
Cuts at: 127 140 1597 1845 127
Size: 13 1457 248 2445
Fragments arranged by size:
2445 1457 248 13
BbsI GAAGACnn'nnnn_
Cuts at: 579 628 579
Size: 49 4114
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 663 697 663
Size: 34 4129
BciVI GTATCCnnnnn_n'
Cuts at: 2061 3117 4163 2061
Size: 1056 1046 2061
Fragments arranged by size:
2061 1056 1046
BclI T'GATC_A
Cuts at: 1683 1683
Size: 4163
BglI GCCn_nnn'nGGC
Cuts at: 427 427
Size: 4163
BglII A'GATC_T
Cuts at: 1678 3715 1678
Size: 2037 2126
BlpI GC'TnA_GC
Cuts at: 198 373 1275 198
Size: 175 902 3086
Fragments arranged by size:
3086 902 175
Bme1580I G_kGCm'C
Cuts at: 652 664 919 1036 1788 1881 3005 652
Size: 12 255 117 752 93 1124 1810
Fragments arranged by size:
1810 1124 752 255 117 93 12
BmrI ACTGGGnnnn_n'
Cuts at: 1375 1456 1791 3935 1375
Size: 81 335 2144 1603
Fragments arranged by size:
2144 1603 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2530 2530
Size: 4163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 408 529 1048 408
Size: 121 519 3523
Fragments arranged by size:
3523 519 121
Bpu10I CC'TnA_GC
Cuts at: 488 488
Size: 4163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1339 2218 2932 3230 1339
Size: 879 714 298 2272
Fragments arranged by size:
2272 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3867 3867
Size: 4163
BsaAI yAC'GTr
Cuts at: 1516 1516
Size: 4163
BsaBI GATnn'nnATC
Cuts at: 6 523 1677 3714 6
Size: 517 1154 2037 455
Fragments arranged by size:
2037 1154 517 455
BsaHI Gr'CG_yC
Cuts at: 124 137 625 1594 1842 2544 4040 124
Size: 13 488 969 248 702 1496 247
Fragments arranged by size:
1496 969 702 488 248 247 13
BsaWI w'CCGG_w
Cuts at: 1352 1873 2551 2962 3109 3383 1352
Size: 521 678 411 147 274 2132
Fragments arranged by size:
2132 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 343 925 343
Size: 582 3581
BseYI C'CCAG_C
Cuts at: 406 693 771 1588 2346 3011 3419 406
Size: 287 78 817 758 665 408 1150
Fragments arranged by size:
1150 817 758 665 408 287 78
BsiEI CG_ry'CG
Cuts at: 104 1705 1751 2981 104
Size: 1601 46 1230 1286
Fragments arranged by size:
1601 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1177 1955 2145 3005 1177
Size: 778 190 860 2335
Fragments arranged by size:
2335 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 652 664 919 1036 1177 1788 1881 1955 2145
Size: 12 255 117 141 611 93 74 190
Cuts at: 2145 2207 3005 652
Size: 62 798 1810
Fragments arranged by size:
1810 798 611 255 190 141 117 93
74 62 12
BspHI T'CATG_A
Cuts at: 2595 2595
Size: 4163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 372 547 685 747 1213 1427 1729 2110 372
Size: 175 138 62 466 214 302 381 2425
Fragments arranged by size:
2425 466 381 302 214 175 138 62
BsrBI CCG'CTC
Cuts at: 50 2455 2509 3993 4158 50
Size: 2405 54 1484 165 55
Fragments arranged by size:
2405 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 792 2075 792
Size: 1283 2880
BsrFI r'CCGG_y
Cuts at: 126 377 2161 2342 126
Size: 251 1784 181 1947
Fragments arranged by size:
1947 1784 251 181
BssSI C'ACGA_G
Cuts at: 2434 3142 2434
Size: 708 3455
BstAPI GCAn_nnn'nTGC
Cuts at: 310 319 1540 310
Size: 9 1221 2933
Fragments arranged by size:
2933 1221 9
BstBI TT'CG_AA
Cuts at: 173 2524 173
Size: 2351 1812
BstEII G'GTnAC_C
Cuts at: 1140 1140
Size: 4163
BstYI r'GATC_y
Cuts at: 1 1013 1188 1655 1678 2013 2259 2565 2577
Size: 1012 175 467 23 335 246 306 12
Cuts at: 2577 2663 3715 1
Size: 86 1052 449
Fragments arranged by size:
1052 1012 467 449 335 306 246 175
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 234 234
Size: 4163
BtgI C'CryG_G
Cuts at: 107 612 911 107
Size: 505 299 3359
Fragments arranged by size:
3359 505 299
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2113 2294 2457 2113
Size: 181 163 3819
Fragments arranged by size:
3819 181 163
BtsI GCAGTG_nn'
Cuts at: 766 877 899 1406 766
Size: 111 22 507 3523
Fragments arranged by size:
3523 507 111 22
DraI TTT'AAA
Cuts at: 1162 1162
Size: 4163
DraIII CAC_nnn'GTG
Cuts at: 1002 1002
Size: 4163
DrdI GACnn_nn'nnGTC
Cuts at: 1149 1869 3213 1149
Size: 720 1344 2099
Fragments arranged by size:
2099 1344 720
EaeI y'GGCC_r
Cuts at: 1748 1922 2313 2340 3685 1748
Size: 174 391 27 1345 2226
Fragments arranged by size:
2226 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1748 1748
Size: 4163
EarI CTCTTCn'nnn_
Cuts at: 320 844 853 973 2186 2396 320
Size: 524 9 120 1213 210 2087
Fragments arranged by size:
2087 1213 524 210 120 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 206 2371 3113 3259 3973 206
Size: 2165 742 146 714 396
Fragments arranged by size:
2165 742 714 396 146
EcoICRI GAG'CTC
Cuts at: 1175 1175
Size: 4163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 344 408 417 529 1048 1988 2420 2773 3435
Size: 64 9 112 519 940 432 353 662
Cuts at: 3435 344
Size: 1072
Fragments arranged by size:
1072 940 662 519 432 353 112 64
9
EcoNI CCTnn'n_nnAGG
Cuts at: 280 280
Size: 4163
EcoO109I rG'GnC_Cy
Cuts at: 428 916 1032 1033 1059 1302 428
Size: 488 116 1 26 243 3289
Fragments arranged by size:
3289 488 243 116 26 1
EcoRI G'AATT_C
Cuts at: 1167 1167
Size: 4163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2182 2182
Size: 4163
FspI TGC'GCA
Cuts at: 883 1944 883
Size: 1061 3102
HaeII r_GCGC'y
Cuts at: 127 140 276 1597 1845 3075 3846 127
Size: 13 136 1321 248 1230 771 444
Fragments arranged by size:
1321 1230 771 444 248 136 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 217 249 2005 2035 2037 2067 217
Size: 32 1756 30 2 30 2313
Fragments arranged by size:
2313 1756 32 30 30 2
HincII GTy'rAC
Cuts at: 537 1202 537
Size: 665 3498
KasI G'GCGC_C
Cuts at: 123 136 1593 1841 123
Size: 13 1457 248 2445
Fragments arranged by size:
2445 1457 248 13
KpnI G_GTAC'C
Cuts at: 1183 1183
Size: 4163
MluI A'CGCG_T
Cuts at: 3596 3596
Size: 4163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2922 3106 2922
Size: 184 3979
MscI TGG'CCA
Cuts at: 1924 1924
Size: 4163
MslI CAynn'nnrTG
Cuts at: 655 2152 2279 655
Size: 1497 127 2539
Fragments arranged by size:
2539 1497 127
MspA1I CmG'CkG
Cuts at: 121 202 307 316 372 927 1274 1588 1948
Size: 81 105 9 56 555 347 314 360
Cuts at: 1948 2732 2977 121
Size: 784 245 1307
Fragments arranged by size:
1307 784 555 360 347 314 245 105
81 56 9
NaeI GCC'GGC
Cuts at: 128 379 2344 128
Size: 251 1965 1947
Fragments arranged by size:
1965 1947 251
NarI GG'CG_CC
Cuts at: 124 137 1594 1842 124
Size: 13 1457 248 2445
Fragments arranged by size:
2445 1457 248 13
NcoI C'CATG_G
Cuts at: 107 612 107
Size: 505 3658
NgoMIV G'CCGG_C
Cuts at: 126 377 2342 126
Size: 251 1965 1947
Fragments arranged by size:
1965 1947 251
NspI r_CATG'y
Cuts at: 966 1216 1716 3319 966
Size: 250 500 1603 1810
Fragments arranged by size:
1810 1603 500 250
PciI A'CATG_T
Cuts at: 3315 3315
Size: 4163
PflMI CCAn_nnn'nTGG
Cuts at: 1002 1002
Size: 4163
PfoI T'CCnGG_A
Cuts at: 506 641 1016 506
Size: 135 375 3653
Fragments arranged by size:
3653 375 135
PmeI GTTT'AAAC
Cuts at: 1162 1162
Size: 4163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2609 2641 2609
Size: 32 4131
PpuMI rG'GwC_Cy
Cuts at: 1059 1059
Size: 4163
PspOMI G'GGCC_C
Cuts at: 915 1032 915
Size: 117 4046
PstI C_TGCA'G
Cuts at: 758 1151 1210 1895 758
Size: 393 59 685 3026
Fragments arranged by size:
3026 685 393 59
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4163
PvuII CAG'CTG
Cuts at: 202 307 316 372 1588 1948 202
Size: 105 9 56 1216 360 2417
Fragments arranged by size:
2417 1216 360 105 56 9
RsrII CG'GwC_CG
Cuts at: 908 2358 908
Size: 1450 2713
SacI G_AGCT'C
Cuts at: 1177 1177
Size: 4163
SalI G'TCGA_C
Cuts at: 1200 1200
Size: 4163
SanDI GG'GwC_CC
Cuts at: 1059 1059
Size: 4163
SapI GCTCTTCn'nnn_
Cuts at: 973 2186 2396 973
Size: 1213 210 2740
Fragments arranged by size:
2740 1213 210
SbfI CC_TGCA'GG
Cuts at: 1210 1210
Size: 4163
ScaI AGT'ACT
Cuts at: 3680 3680
Size: 4163
SfcI C'TryA_G
Cuts at: 33 116 754 1147 1206 1891 2859 3050 33
Size: 83 638 393 59 685 968 191 1146
Fragments arranged by size:
1146 968 685 638 393 191 83 59
SfoI GGC'GCC
Cuts at: 125 138 1595 1843 125
Size: 13 1457 248 2445
Fragments arranged by size:
2445 1457 248 13
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
SgrAI Cr'CCGG_yG
Cuts at: 126 126
Size: 4163
SmaI CCC'GGG
Cuts at: 1185 1185
Size: 4163
SmlI C'TyrA_G
Cuts at: 1318 2233 2947 3209 1318
Size: 915 714 262 2272
Fragments arranged by size:
2272 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4163
SphI G_CATG'C
Cuts at: 1216 1716 1216
Size: 500 3663
StyI C'CwwG_G
Cuts at: 107 612 1297 1651 107
Size: 505 685 354 2619
Fragments arranged by size:
2619 685 505 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 247 853 1746 1879 2338 2547 247
Size: 606 893 133 459 209 1863
Fragments arranged by size:
1863 893 606 459 209 133
TatI w'GTAC_w
Cuts at: 3678 3678
Size: 4163
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3680 3712 3680
Size: 32 4131
Tth111I GACn'n_nGTC
Cuts at: 1960 1960
Size: 4163
XbaI T'CTAG_A
Cuts at: 1194 1194
Size: 4163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 882 1658 882
Size: 776 3387
XmaI C'CCGG_G
Cuts at: 1183 1183
Size: 4163
ZraI GAC'GTC
Cuts at: 626 626
Size: 4163
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AleI AlfI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI BsaXI BsgI BsiWI
BsmI BsmBI BspEI BsrGI BssHII BstXI BstZ17I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PsiI PspXI PsrI SacII SexAI SfiI SnaBI SrfI
SspI StuI SwaI XhoI XmnI