Restriction map for
pF1KB8604
(Circular) (Six-base) MAPSORT of: pF1KB8604.seq Check: 8033 from: 1 to: 4352
pF1KB8604 4352 bp
With 182 enzymes: SgfI *
February 20, 2009 16:36 ..
AatII G_ACGT'C
Cuts at: 496 563 496
Size: 67 4285
AccI GT'mk_AC
Cuts at: 1390 1390
Size: 4352
Acc65I G'GTAC_C
Cuts at: 1368 1368
Size: 4352
AclI AA'CG_TT
Cuts at: 1346 4192 1346
Size: 2846 1506
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 979 1129 2177 2609 2962 3624 730
Size: 249 150 1048 432 353 662 1458
Fragments arranged by size:
1458 1048 662 432 353 249 150
AflIII A'CryG_T
Cuts at: 1704 3504 3785 1704
Size: 1800 281 2271
Fragments arranged by size:
2271 1800 281
AleI CACnn'nnGTG
Cuts at: 514 514
Size: 4352
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 532 1018 532
Size: 486 3866
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 523 555 874 906 523
Size: 32 319 32 3969
Fragments arranged by size:
3969 319 32 32
AlwNI CAG_nnn'CTG
Cuts at: 573 912 1657 3095 573
Size: 339 745 1438 1830
Fragments arranged by size:
1830 1438 745 339
ApaI G_GGCC'C
Cuts at: 321 321
Size: 4352
ApaLI G'TGCA_C
Cuts at: 3190 3190
Size: 4352
ApoI r'AATT_y
Cuts at: 1356 3705 3961 1356
Size: 2349 256 1747
Fragments arranged by size:
2349 1747 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4352
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4352
AvaI C'yCGr_G
Cuts at: 169 326 352 1372 169
Size: 157 26 1020 3149
Fragments arranged by size:
3149 1020 157 26
BamHI G'GATC_C
Cuts at: 1377 1377
Size: 4352
BanI G'GyrC_C
Cuts at: 1255 1368 1782 2030 2065 1255
Size: 113 414 248 35 3542
Fragments arranged by size:
3542 414 248 113 35
BanII G_rGCy'C
Cuts at: 137 321 353 359 667 1148 1366 2396 137
Size: 184 32 6 308 481 218 1030 2093
Fragments arranged by size:
2093 1030 481 308 218 184 32 6
BbeI G_GCGC'C
Cuts at: 1786 2034 1786
Size: 248 4104
BbsI GAAGACnn'nnnn_
Cuts at: 427 718 427
Size: 291 4061
BciVI GTATCCnnnnn_n'
Cuts at: 2250 3306 4352 2250
Size: 1056 1046 2250
Fragments arranged by size:
2250 1056 1046
BclI T'GATC_A
Cuts at: 1872 1872
Size: 4352
BglI GCCn_nnn'nGGC
Cuts at: 653 856 653
Size: 203 4149
BglII A'GATC_T
Cuts at: 1867 3904 1867
Size: 2037 2315
BlpI GC'TnA_GC
Cuts at: 305 1464 305
Size: 1159 3193
Bme1580I G_kGCm'C
Cuts at: 321 1246 1977 2070 3194 321
Size: 925 731 93 1124 1479
Fragments arranged by size:
1479 1124 925 731 93
BmgBI CAC'GTC
Cuts at: 600 600
Size: 4352
BmrI ACTGGGnnnn_n'
Cuts at: 1564 1645 1980 4124 1564
Size: 81 335 2144 1792
Fragments arranged by size:
2144 1792 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2719 2719
Size: 4352
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 520 718 757 852 1027 292
Size: 228 198 39 95 175 3617
Fragments arranged by size:
3617 228 198 175 95 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1528 2407 3121 3419 1528
Size: 879 714 298 2461
Fragments arranged by size:
2461 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1183 4056 1183
Size: 2873 1479
BsaAI yAC'GTr
Cuts at: 1705 1705
Size: 4352
BsaBI GATnn'nnATC
Cuts at: 6 1866 3903 6
Size: 1860 2037 455
Fragments arranged by size:
2037 1860 455
BsaHI Gr'CG_yC
Cuts at: 493 560 1783 2031 2733 4229 493
Size: 67 1223 248 702 1496 616
Fragments arranged by size:
1496 1223 702 616 248 67
BsaWI w'CCGG_w
Cuts at: 776 1202 1541 2062 2740 3151 3298 3572 776
Size: 426 339 521 678 411 147 274 1556
Fragments arranged by size:
1556 678 521 426 411 339 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 523 553 523
Size: 30 4322
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 229 238 241 250 313 538 910 913 229
Size: 9 3 9 63 225 372 3 3668
Fragments arranged by size:
3668 372 225 63 9 9 3 3
BseYI C'CCAG_C
Cuts at: 363 1061 1212 1222 1777 2535 3200 3608 363
Size: 698 151 10 555 758 665 408 1107
Fragments arranged by size:
1107 758 698 665 555 408 151 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 538
Size: 4352
BsiEI CG_ry'CG
Cuts at: 104 1894 1940 3170 104
Size: 1790 46 1230 1286
Fragments arranged by size:
1790 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1013 1148 1186 1366 2144 2334 3194 1013
Size: 135 38 180 778 190 860 2171
Fragments arranged by size:
2171 860 778 190 180 135 38
BsmBI CGTCTCn'nnnn_
Cuts at: 601 601
Size: 4352
Bsp1286I G_dGCh'C
Cuts at: 137 321 353 359 667 1013 1148 1186 1246
Size: 184 32 6 308 346 135 38 60
Cuts at: 1246 1366 1977 2070 2144 2334 2396 3194 137
Size: 120 611 93 74 190 62 798 1295
Fragments arranged by size:
1295 798 611 346 308 190 184 135
120 93 74 62 60 38 32 6
BspHI T'CATG_A
Cuts at: 2784 2784
Size: 4352
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1402 1616 1918 2299 1402
Size: 214 302 381 3455
Fragments arranged by size:
3455 381 302 214
BsrBI CCG'CTC
Cuts at: 50 168 2644 2698 4182 4347 50
Size: 118 2476 54 1484 165 55
Fragments arranged by size:
2476 1484 165 118 55 54
BsrDI GCAATG_nn'
Cuts at: 2264 2264
Size: 4352
BsrFI r'CCGG_y
Cuts at: 1226 2350 2531 1226
Size: 1124 181 3047
Fragments arranged by size:
3047 1124 181
BssSI C'ACGA_G
Cuts at: 2623 3331 2623
Size: 708 3644
BstAPI GCAn_nnn'nTGC
Cuts at: 118 1729 118
Size: 1611 2741
BstBI TT'CG_AA
Cuts at: 2713 2713
Size: 4352
BstEII G'GTnAC_C
Cuts at: 474 702 474
Size: 228 4124
BstXI CCAn_nnnn'nTGG
Cuts at: 383 1277 383
Size: 894 3458
BstYI r'GATC_y
Cuts at: 1 576 1377 1844 1867 2202 2448 2754 2766
Size: 575 801 467 23 335 246 306 12
Cuts at: 2766 2852 3904 1
Size: 86 1052 449
Fragments arranged by size:
1052 801 575 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1096 1096
Size: 4352
BtgI C'CryG_G
Cuts at: 107 344 614 1270 107
Size: 237 270 656 3189
Fragments arranged by size:
3189 656 270 237
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1317 2302 2483 2646 1317
Size: 985 181 163 3023
Fragments arranged by size:
3023 985 181 163
BtsI GCAGTG_nn'
Cuts at: 145 620 1595 145
Size: 475 975 2902
Fragments arranged by size:
2902 975 475
DraI TTT'AAA
Cuts at: 1351 1351
Size: 4352
DrdI GACnn_nn'nnGTC
Cuts at: 559 2058 3402 559
Size: 1499 1344 1509
Fragments arranged by size:
1509 1499 1344
EaeI y'GGCC_r
Cuts at: 611 770 827 1937 2111 2502 2529 3874 611
Size: 159 57 1110 174 391 27 1345 1089
Fragments arranged by size:
1345 1110 1089 391 174 159 57 27
EagI C'GGCC_G
Cuts at: 1937 1937
Size: 4352
EarI CTCTTCn'nnn_
Cuts at: 215 227 236 374 1042 2375 2585 215
Size: 12 9 138 668 1333 210 1982
Fragments arranged by size:
1982 1333 668 210 138 12 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 310 829 2560 3302 3448 4162 310
Size: 519 1731 742 146 714 500
Fragments arranged by size:
1731 742 714 519 500 146
EcoICRI GAG'CTC
Cuts at: 1146 1364 1146
Size: 218 4134
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 520 718 730 757 852 979 1027 1129
Size: 228 198 12 27 95 127 48 102
Cuts at: 1129 2177 2609 2962 3624 292
Size: 1048 432 353 662 1020
Fragments arranged by size:
1048 1020 662 432 353 228 198 127
102 95 48 27 12
EcoO109I rG'GnC_Cy
Cuts at: 159 317 318 570 1491 159
Size: 158 1 252 921 3020
Fragments arranged by size:
3020 921 252 158 1
EcoRI G'AATT_C
Cuts at: 1356 1356
Size: 4352
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1053 2371 1053
Size: 1318 3034
FspI TGC'GCA
Cuts at: 2133 2133
Size: 4352
HaeII r_GCGC'y
Cuts at: 875 1786 2034 3264 4035 875
Size: 911 248 1230 771 1192
Fragments arranged by size:
1230 1192 911 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2194 2224 2226 2256 2194
Size: 30 2 30 4290
Fragments arranged by size:
4290 30 30 2
HincII GTy'rAC
Cuts at: 1391 1391
Size: 4352
KasI G'GCGC_C
Cuts at: 1782 2030 1782
Size: 248 4104
KpnI G_GTAC'C
Cuts at: 1372 1372
Size: 4352
MluI A'CGCG_T
Cuts at: 3785 3785
Size: 4352
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1148 3111 3295 1148
Size: 1963 184 2205
Fragments arranged by size:
2205 1963 184
MscI TGG'CCA
Cuts at: 772 2113 772
Size: 1341 3011
MslI CAynn'nnrTG
Cuts at: 514 598 1275 2341 2468 514
Size: 84 677 1066 127 2398
Fragments arranged by size:
2398 1066 677 127 84
MspA1I CmG'CkG
Cuts at: 270 810 957 1179 1463 1777 2137 2921 3166
Size: 540 147 222 284 314 360 784 245
Cuts at: 3166 270
Size: 1456
Fragments arranged by size:
1456 784 540 360 314 284 245 222
147
NaeI GCC'GGC
Cuts at: 1228 2533 1228
Size: 1305 3047
NarI GG'CG_CC
Cuts at: 1783 2031 1783
Size: 248 4104
NcoI C'CATG_G
Cuts at: 107 344 614 1270 107
Size: 237 270 656 3189
Fragments arranged by size:
3189 656 270 237
NgoMIV G'CCGG_C
Cuts at: 1226 2531 1226
Size: 1305 3047
NspI r_CATG'y
Cuts at: 1171 1405 1905 3508 1171
Size: 234 500 1603 2015
Fragments arranged by size:
2015 1603 500 234
PasI CC'CwG_GG
Cuts at: 433 433
Size: 4352
PciI A'CATG_T
Cuts at: 3504 3504
Size: 4352
PflMI CCAn_nnn'nTGG
Cuts at: 573 1271 573
Size: 698 3654
PmeI GTTT'AAAC
Cuts at: 1351 1351
Size: 4352
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 523 555 2798 2830 523
Size: 32 2243 32 2045
Fragments arranged by size:
2243 2045 32 32
PpuMI rG'GwC_Cy
Cuts at: 159 159
Size: 4352
PshAI GACnn'nnGTC
Cuts at: 427 427
Size: 4352
PspOMI G'GGCC_C
Cuts at: 317 317
Size: 4352
PspXI vC'TCGA_Gb
Cuts at: 169 169
Size: 4352
PstI C_TGCA'G
Cuts at: 491 917 997 1399 2084 491
Size: 426 80 402 685 2759
Fragments arranged by size:
2759 685 426 402 80
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4352
PvuII CAG'CTG
Cuts at: 957 1777 2137 957
Size: 820 360 3172
Fragments arranged by size:
3172 820 360
RsrII CG'GwC_CG
Cuts at: 2547 2547
Size: 4352
SacI G_AGCT'C
Cuts at: 1148 1366 1148
Size: 218 4134
SalI G'TCGA_C
Cuts at: 1389 1389
Size: 4352
SanDI GG'GwC_CC
Cuts at: 159 159
Size: 4352
SapI GCTCTTCn'nnn_
Cuts at: 2375 2585 2375
Size: 210 4142
SbfI CC_TGCA'GG
Cuts at: 1399 1399
Size: 4352
ScaI AGT'ACT
Cuts at: 637 3869 637
Size: 3232 1120
SfcI C'TryA_G
Cuts at: 33 487 913 993 1395 2080 3048 3239 33
Size: 454 426 80 402 685 968 191 1146
Fragments arranged by size:
1146 968 685 454 426 402 191 80
SfoI GGC'GCC
Cuts at: 1784 2032 1784
Size: 248 4104
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4352
SmaI CCC'GGG
Cuts at: 1374 1374
Size: 4352
SmlI C'TyrA_G
Cuts at: 169 1507 2422 3136 3398 169
Size: 1338 915 714 262 1123
Fragments arranged by size:
1338 1123 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4352
SphI G_CATG'C
Cuts at: 1171 1405 1905 1171
Size: 234 500 3618
Fragments arranged by size:
3618 500 234
StyI C'CwwG_G
Cuts at: 107 344 614 651 1270 1304 1486 1840 107
Size: 237 270 37 619 34 182 354 2619
Fragments arranged by size:
2619 619 354 270 237 182 37 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 538 1935 2068 2527 2736 538
Size: 1397 133 459 209 2154
Fragments arranged by size:
2154 1397 459 209 133
TatI w'GTAC_w
Cuts at: 635 1073 3867 635
Size: 438 2794 1120
Fragments arranged by size:
2794 1120 438
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3869 3901 3869
Size: 32 4320
Tth111I GACn'n_nGTC
Cuts at: 820 2149 820
Size: 1329 3023
XbaI T'CTAG_A
Cuts at: 1383 1383
Size: 4352
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 876 1847 876
Size: 971 3381
XhoI C'TCGA_G
Cuts at: 169 169
Size: 4352
XmaI C'CCGG_G
Cuts at: 1372 1372
Size: 4352
ZraI GAC'GTC
Cuts at: 494 561 494
Size: 67 4285
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmI BspEI
BsrGI BssHII BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PfoI PmlI PsiI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XmnI